Short-term reliability of inflammatory mediators and response to exercise in the heat     

Joshua H. Guy  Andrew M. Edwards  Catherine M. Miller  Glen B. Deakin  David B. Pyne 《Journal of sports sciences》2017,35(16):1622-1628

Prospective application of serum cytokines, lipopolysaccharide (LPS), and heat shock proteins (eHSPs) requires reliable measurement of these biomarkers that can signify exercise-induced heat stress in hot conditions. To accomplish this, both short-term (7 day) reliability (at rest, n = 12) and the acute responsiveness of each biomarker to exercise in the heat (pre and post 60-min cycling, 34.5°C and 70% RH, n = 20) were evaluated. Serum was analysed for the concentration of C-reactive protein (CRP), interleukin-6 (IL-6), heat shock protein 72 (eHSP72), immunoglobulin M (IgM) and LPS. Test–retest reliability was determined as the coefficient of variation (CV). Biomarkers with the least short-term within-participant variation were IL-6 (19%, ±20%; CV, ±95% confidence limits (CL)) and LPS (23%, ±13%). Greater variability was observed for IgM, eHSP72 and CRP (CV range 28–38%). IL-6 exhibited the largest increase in response to acute exercise (95%, ±11%, P = < 0.001) and although CRP had a modest CV (12%, ±7%), it increased substantially post-exercise (P = 0.02, ES; 0.78). In contrast, eHSP72 and LPS exhibited trivial changes post-exercise. It appears variation of common inflammatory markers after exercise in the heat is not always discernible from short-term (weekly) variation.  相似文献   

快速定量分析牛乳中α-乳白蛋白的竞争酶联免疫吸附法     

宋宏新  程妮  薛海燕  杜枘宣 《中国科学院大学学报》2016,33(3):360-364

建立一种灵敏度高和特异性好的直接竞争ELISA法来定性和定量检测牛乳中α-乳白蛋白(α-LA).通过使用牛乳α-LA多次免疫新西兰大白兔制备多克隆抗体,并采用过碘酸钠氧化法制备辣根过氧化物酶(HRP)标记的α-LA,建立检测α-LA的直接竞争ELISA法.所制备的多克隆抗体特异性较高,无明显交叉反应,效价为1:80 920.酶标抗原的标记率达66.67%.直接竞争ELISA法标准曲线相关系数为0.998 57,回收率在86.00%~117.50%之间,重复性高,变异系数<5%.与国标电泳方法进行样品检测比较,结果显示该方法可有效地用于食品中α-LA的快速定性和定量检测.  相似文献   

嗜水气单胞菌外分泌物的致病性   总被引：3，自引：0，他引：3  

邱德全  何建国  钟英长  江静波 《中山大学学报论丛》1996,(Z1)

该菌分泌物有溶血性、细胞毒性和肠毒素毒性，毒性实验证实嗜水气单胞菌外分泌物对小白鼠和中华鳖有较高的致死毒性．层析纯化得到的1种蛋白质有上述3种毒性和致死性．  相似文献   

A biochemical study on the effect of Riboflavin on cataractous rat lenses. II Proteins and Glutathione     

Rita R. Vani  U. M. Rawal 《Indian journal of clinical biochemistry : IJCB》1994,9(2):96-97

Proteins and glutathione (GSH) levels were estimated in the experimental groups. Levels of both proteins and glutathione in the control and the treated groups were found to be significantly decreased compared to the normal group. The results show that supplementation of riboflavin has beneficial effect by alleviating the oxidant injuries to GSH and proteins, which are involved in the maintenance of transparency and protection of thiol groups of the lens. Thus, riboflavin has an active role in the lens metabolism and has a potential to improve the metabolic activity of the cataractous lenses.  相似文献   

骨骼肌热休克蛋白变化研究进展     

高前进  王二利  王瑞元 《北京体育大学学报》2007,30(7):941-943

在应激状态,细胞快速合成一组新的蛋白质,称为热休克蛋白.研究表明,骨骼肌含有多种热休克蛋白.热休克蛋白随肌纤维类型不同而有不同的表达,表明热休克蛋白表达具有肌纤维类型特异性.骨骼肌疾病和运动都能诱导热休克蛋白表达增强,然而热休克蛋白在骨骼肌内的诱导、调节的分子机制和它在维持骨骼肌功能方面的作用还没有被完全理解.对骨骼肌热休克蛋白在生理和病理情况下的改变进行综述.  相似文献   

基于序列及结构特征的固有无序蛋白—核酸相互作用位点预测分析     

王洪波郭珍珍  于家峰等 《德州学院学报》2014,(2):6-10

固有无序蛋白是一类具有柔性结构的蛋白质,许多情况下通过与其它物质相互作用形成稳定结构来发挥重要生物功能.本文分别利用基于序列特征和结构特征的蛋白质结合位点预测程序对固有无序蛋白有序区和无序区与核酸分子的结合位点进行了预测分析.结果发现,基于结构特征的结合位点预测方法整体上要优于基于序列特征的预测方法,表明无序区尽管缺少稳定空间结构,结构特征依然在固有无序蛋白结合位点中发挥了重要作用.因此,可以为今后固有无序蛋白序列特征及结构特征的提取提供可靠依据.  相似文献   

Enhanced expression of HMG-Y proteins in proliferating tissues     

Dinesh Singh  M. R. Rajeswari 《Indian journal of clinical biochemistry : IJCB》2001,16(1):72-76

The high mobility group (HMG) proteins I and Y are well characterised non-histone chromosomal proteins which bind to A-T rich regions of DNA and regulate gene expression and/or DNA replication. A correlation has been demonstrated between the increased expression of HMG-Y proteins and malignancy. However, it is not known whether the expression of HMGs particularly, the Y group, is a function of proliferation rate. In the present study, we have used normal tissues of calf testes, thymus and liver. The results show distinctly high expression of HMG-Y proteins in testes than in thymus and the expression was practically undetectable in liver. The results suggest that even in normal tissues there is a direct correlation between the proliferation rate and the expression of the HMG-Y proteins, which can partly explain its increased expression in cancer.  相似文献   

热休克蛋白研究进展   总被引：1，自引：0，他引：1  

郑光宇 《喀什师范学院学报》2003,24(6):56-59

热休克蛋白是细胞内高度保守的，而且最丰富的一类蛋白。作为分子伴侣，热休克蛋白参与蛋白的折叠与去折叠、装配、运输和降解．近年来研究发现，热休克蛋白中的一些成员，例如HSP70、HSP90和gp96，可以将与之结合的抗原肽递呈给MHCⅠ类分子，并激活特异性CTL。  相似文献   

对吉林省男子速滑运动员赛前营养补充方案和监测指标变化的研究     

张迪  王露 《吉林体育学院学报》2010,26(4):90-91

通过监测吉林省速滑运动员赛前训练的生理生化指标（血红蛋白、血尿素、肌酸激酶、尿蛋白等）变化,来调整和确定训练的营养补充方案,进一步探讨营养生化监控和科学训练的关系,并发现赛前全队平均血红蛋白浓度、血尿素浓度和肌酸激酶指标都处于较理想范围;全队尿蛋白浓度和尿蛋白阳性检出率都明显下降。说明赛前训练改善了运动员的体能状况和运动员对训练负荷的适应情况良好。  相似文献   

Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate     

Li-rong CHEN  Hong-wei ZHOU  Jia-chang CAI  Rong ZHANG  Gong-xiang CHEN 《Journal of Zhejiang University. Science. B》2009,10(5)

Objective: To investigate the mechanism of carbapenem resistance and the occurrence of plasmid-mediated quinolone resistance determinants qnr and aac(6')-Ib-cr in a clinical isolate of Enterobacter cloacae. Methods: An ertapenem-resistant E. cloacae ZY106, which was isolated from liquor puris of a female gastric cancer patient in a Chinese hospital, was investigated. Antibiotic susceptibilities were determined by agar dilution method. Conjugation experiments, isoelectric focusing, polymerase chain reaction (PCR), and DNA sequence analyses of plasmid-mediated carbapenemases and quinolone resistance determinants were preformed to confirm the genotype. Outer membrane proteins (OMPs) were examined by urea-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Urea-SDS-PAGE). Results: Minimum inhibitory concentrations (MCs) of imipenem, mer-openem, and ertapenem for ZY106 were 2,4, and 16 ug/ml, respectively. Conjugation studies with Escherichia coli resulted in the transfer of significantly reduced carbapenem susceptibility. ZY106 produced IMP-1 metallo-p-lactamase and CTX-M-3 extended-spectrum P-lactamase, and E. coli transconjugant produced IMP-1. Plasmid-mediated quinolone resistance determinant qnrSI was detected in ZY106. Transfer of the qnrSI-encoding-plasmid into E. coli by conjugation resulted in intermediate resistance to ciprofloxacin in E. coli transconjugant. Urea-SDS-PAGE analysis of OMPs showed that ZY106 lacked an OMP of approximately 38 KDa. Conclusion: It is the first IMP-1-producing Enterobacteriaceae in China and the first report of a clinical isolate that harbors both blaIMP and qnrS genes as well. The blaIMP-1, blaCTX-M-3, and qnrSl are encoded at three different plasmids. IMP-1 combined with the loss of an OMP possibly resulted in ertapenem resistance and reduced imipenem and mero-penem susceptibility in E. cloacae.  相似文献