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本文采用A-PAGE方法,对外源DNA导入两次的四个后代变异系、一个后代变异株系的六个单株和导入后代间杂交的四个后代变异系进行了醇溶蛋白电泳分析。结果表明:1.外源DNA两次导入进一步强化了变异;2.A-PAGE方法不仅可用于验证外源DNA导入的变异后代,而且可检测后代变异系的遗传稳定性;3.使外源DNA导入后的变异后代间再进行杂交,能够丰富变异类型。而且导入技术与杂交方法的结合,可以互相补充,对作物育种具有重要意义。  相似文献   
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Recent events have renewed attention to how organizations rely on digital resources in response to exogenous shock. Though the literature on organizational resilience indicates that this is best understood as a process through which organizational actors respond to a specific shock, most IS research attends to resilience as an outcome. Against that backdrop, we present a case study of how a university shifted to virtual teaching in response to a government-imposed lockdown during the COVID-19 pandemic. Adopting a digital resourcing perspective allowed us to reveal the organizational resilience process and the way digital resources shaped it. We found that the resilience process unfolded in stages as educators, assisted by students, managers, and IT personnel pivoted, adapted, and normalized into teaching virtually. Across these stages, digital resources took on specific roles as the resilience process progressed from the organization’s pre-shock accumulation of digital resources into its continued digitalization efforts. Based on these findings, we contribute to existing literature by advancing and empirically substantiating a process view of the role of digital resources in organizational resilience.  相似文献   
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论现代远程教育伦理责任的两个维度   总被引:1,自引:0,他引:1  
在对远程教育伦理责任的研究中,人们通常从公正、公平等角度来研究,这是社会伦理基本要求,是一种外生式伦理责任,具有其相应的规定性。但远程教育在发展的同时,又表现出自身的特性,是一种内含式的伦理责任,它依附于外生式伦理责任,且不同于外生式伦理责任。  相似文献   
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The identification of emerging topics is of current interest to decision makers in both government and industry. Although many case studies present retrospective analyses of emerging topics, few studies actually nominate emerging topics for consideration by decision makers. We present a novel approach to identifying emerging topics in science and technology. Two large scale models of the scientific literature, one based on direct citation, and the other based on co-citation, are combined to nominate emerging topics using a difference function that rewards clusters that are new and growing rapidly. The top 25 emergent topics are identified for each year 2007 through 2010. These topics are classified and characterized in various ways in order to understand the motive forces behind their emergence, whether scientific discovery, technological innovation, or exogenous events. Topics are evaluated by searching for recent major awards associated with the topic or its key researchers. The evidence presented suggests that the methodology nominates a viable list of emerging topics suitable for inspection by decision makers.  相似文献   
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为研究航运服务集聚区的发展演化机理,在对服务集聚区研究分析的基础上,对航运服务业的类别,航运服务集聚区的内涵、发展演化机理、发展演化阶段、发展演化的分析框架和发展演化动力进行较为系统的定性分析.通过上海市北外滩航运服务集聚区案例对航运服务集聚区的发展演化机理进行实证分析,并在此基础上提出我国航运服务集聚区的未来发展思路...  相似文献   
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基于外部冲击、风险偏好视角,采用调研获取的农户数据,运用Heckman样本选择模型实证分析农户对低碳农业技术的决策选择与采用程度的影响因素,并引入交互项检验风险偏好对外部冲击的调节效应。研究结果表明:当前农户低碳农业技术采用率较低,风险厌恶程度较高者对各项低碳技术的采用率更低;外部冲击、风险偏好均负向显著影响农户采用低碳农业技术的决策选择及采用程度,风险偏好对外部冲击有增强型调节效应,即风险偏好强化外部冲击对低碳农业技术采用的负效应,通过增加农户对风险和不确定性的敏感程度从而间接负向影响农户对低碳农业技术的采用;个体特征、家庭特征对农户低碳农业技术决策选择和采用程度两阶段的影响同中存异,均受到是否党员或村干部的影响,而在性别、年龄、劳动力人数方面存在差异。  相似文献   
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在政府部门的强势引导下,英国高校建立起了内部绩效管理体系。依据高校绩效管理模式的不同来源,可以划分为内生性与外源性两大类。本文在对英国高校绩效管理改革背景进行梳理的基础上,对两类绩效管理模式的运作框架及其特点进行了分析,并结合个案对两类模式的整合进行了初步的探讨,以期为我国正在推进的高校绩效管理改革提供借鉴。  相似文献   
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梁顺祥 《青海科技》2000,7(3):11-13
彩和PAGE方法,对从导入珍珠粟DNA的两个春小麦受体(佛约28和青春4425)中所选的17个变异系进行了酯酶同工酶分析,结果表明,变异系与受体的酯酶同工酶图谱基本相同;同时,在变异系中出现了供体酶带和新酶带,而且在酶活性和酶带数等方面也表现出不同程度的变异。说明酯酶同工酶的变异与粟DNA导入有密切关系,变异系是外源DNA导入的结果,进上步说明花粉管通道法导入外源DNA技术是改良小麦品种、丰富小麦  相似文献   
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