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目的:碱性成纤维细胞生长因子(bFGF)有强烈诱导血管生成和修复血管损伤的作用,故其在心肌中的表达程度可以作为判断运动对心脏侧枝血管生成影响的指标.以雄性SD大鼠为实验对象,通过施加中等强度的游泳训练,观察运动训练对SD大鼠心肌组织学结构和bFGF表达的影响,为运动医学与心脏康复的理论和实践提供依据;方法:将32只健康雄性SD大鼠按体重随机分为2组:对照组(C组);运动组(E组),每组均为16只.C组安静饲养,不进行运动训练,但需放入过膝浅水中以模拟训练组环境;E组大鼠每周训练6天,每天训练1次.从第1周开始适应性游泳训练,第一次游泳5min,此后逐日累加5min,至第3周末时每天游满2h.此后,从第4周起维持这一运动量直至第8周.第8周进行游泳训练后,即刻处死各组大鼠,取出心脏称湿重以做心脏系数统计,并切取左心室前壁组织块,做HE染色和bRGF免疫组织化学染色.用光学显微镜观察各组心肌及心肌间质的变化,并用图像采集分析系统观测各组HE染色和bFGF免疫组织化学染色变化,做统计学处理;实验结果:1)一般情况观察:心脏重量和心系数,E组较C组显著增加(P＜0.01);2)心肌组织学观察,HE染色显示C组心肌组织形态正常;而E组呈现生理性肥大变化,心脏毛细血管功能结构产生了良好的重构,心肌组织之间毛细血管的密度增加;3)bFGF免疫组织化学染色结果观察,C组呈弱阳性表达;E组呈较强的阳性表达;心肌细胞中bFGF表达呈特异性棕黄染色颗粒,以计算机显微图像采集分析系统计算bFGF染色面积,发现E组大于C组(P＜0.01);bFGF染色平均密度E组大于C组(P＜0.01);bFGF染色积分光密度,E组均大于C组(P＜0.01);结论:本实验观察到,适宜的耐力运动训练可以使心肌细胞、心肌血管内皮细胞等部位bFGF的表达增强,改善心肌细胞的分子生物学特性、心脏毛细血管功能结构产生了良好的重构,心肌组织之间毛细血管的密度增加,毛细血管腔表面积密度增高,毛细血管与心肌纤维的比例增大,使心脏产生适应性生理肥大,表明中等强度的运动可以使心脏发生生理性肥大,其机理可能与bFGF的表达增强有关. 相似文献
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探讨模拟高住低训对小鼠肺VEGF、bFGF的影响。结论：小鼠肺组织的VEGFmRNA、bFGFmRNA表达水平都呈上升的表现。bFGFmRNA的增加可能促使VEGFmRNA表达的升高,肺组织毛细血管增生,改善肺组织的低氧状态,提高呼吸系统功能。 相似文献
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李骁君 《天津体育学院学报》2011,26(1):34-36
利用纳米磁性颗粒将bFGF定位于大鼠骨骼肌钝挫伤部位,考察该方法对大鼠急性骨骼肌钝挫伤恢复过程中收缩应力及MHC-Ⅱb表达影响。研究结果表明,bFGF对大鼠腓肠肌钝挫伤后第17、24天的收缩力有明显提高。损伤后第2～10天,bFGF包裹组与其余各组之间的MHC-ⅡbmRNA表达差异显著(P<0.05)。因此,磁纳米化的bFGF能明显改善大鼠急性骨骼肌钝挫伤后的收缩应力衰减,显著促进损伤肌肉MHC-ⅡbmRNA的表达,改善损伤肌肉再生和结构修复。 相似文献
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ＩＮＴＲＯＤＵＣＴＩＯＮＦｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ (ＦＧＦ)ｉｓａｎａｎｇｉｏ ｇｅｎｉｃａｎｄｍｉｔｏｇｅｎｉｃｐｏｌｙｐｅｐｔｉｄｅｔｈａｔｃａｎｐｒｏｍｏｔｅｃｅｌｌｐｒｏｌｉｆｅｒａｔｉｏｎａｎｄｄｉｆｆｅｒｅｎｔｉａｔｉｏｎｉｎａｗｉｄｅｖａ ｒｉｅｔｙｏｆｃｅｌｌｔｙｐｅｓ (Ｇｏｌｄｆａｒｂ ,1 990 ;Ｂａｓｉｌｉｃｏｅｔａｌ.,1 992 ) .ＴｈｅＦＧＦｆａｍｉｌｙｈａｓｍａｎｙｍｅｍｂｅｒｓｗｉｔｈｓｉｍｉｌａｒａｍｉｎｏａｃｉｄｓｅｑｕｅｎｃｅａｎｄｂｉｏｌｏｇｉｃａｌａ… 相似文献
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Effect of basic fibroblast growth factor （bFGF） on the treatment of exposure of the orbital implants 总被引：5，自引：0，他引：5
Objective： To evaluate the efficacy and the indication of basic fibroblast growth factor （bFGF） in the treatment of exposure of orbital implants. Design： Retrospective and observational case series. Methods： We reviewed 41 patients （41 eyes） suffering exposure of orbital implants from Jan. 2000 to June 2006. The study group patients with mild exposure received combined treatment with bFGF and antibiotic drops, and while the control group patients with mild exposure were treated with antibiotic drops only. The study group patients with moderate and severe exposure received combined treatment with bFGF and antibiotic drops, and after 2 months they were subjected to amniotic membrane transplantation, while the control group patients with moderate and severe exposure underwent amniotic membrane transplantation after using antibiotic drops. Observation of the growth of conjunctival epithelium and comparison of the healing rate of the two groups. Results： The healing rates of the mild, moderate and severe exposure study group were 100% and 92.3%. The healing rates of the mild, moderate and severe exposure control group were 55.6% and 66.7% respectively. The difference of the healing rates of the mild exposure study group and the control group was significant （P=-0.033）. And the difference of the healing rates of the moderate and severe exposure study group and the control group was not significant （P=-0.167）. Conclusion： bFGF may promote obviously the healing of orbital implant exposure, particularly it can be the first choice for the treatment of mild degree exposure. For the moderate and severe cases, it can be administered before surgical repair to enhance neovascularization and will tend to increase the success rate of surgical repair. 相似文献
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目的:观察适应性训练、补充海洋肽以及大豆蛋白后骨骼肌bFGF及desmin含量的变化,研究运动训练、补充海洋肽以及大豆蛋白对运动性肌肉损伤和修复的影响。方法:以雄性SD大鼠72只,随机分为:安静组、运动组、运动+海洋肽组、运动+大豆蛋白组。进行6周适应性离心运动训练即-5°下坡间歇跑,跑速从20.0 m/min递增至35.0 m/min,每次10~20 min,每周训练5 d,之后给予定量离心负荷,分别取运动后即刻、24 h和48 h不同时间处死、取股中间肌、测试。结果:6周适应性训练可以增强desmin的适应能力。补充海洋肽组股中间肌desmin蛋白丢失较少。补充大豆蛋白组运动后即刻bFGF蛋白表达量显著性低于安静组,运动后48 h组bFGF蛋白表达量显著性高于运动后即刻组。结论:补充海洋肽能够更好的减少离心运动后骨架蛋白的丢失,促进运动后骨骼肌损伤的修复。 相似文献
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Human acidic and basic fibroblast growth factors (aFGF and bFGF) are classic and well characterized members of the heparin-binding
growth factor family. Heparin is generally thought to play an extremely important role in regulating aFGF and bFGF bioactivities
through its strong binding with them. In order to unravel the mechanism of the interactions between heparin and FGFs, and
evaluate the importance of heparin sulfate groups' binding with FGFs, surface plasmon resonance analyses were performed using
IAsys Cuvettes System. Heparin and its regioselectively desulfated derivatives were immobilized on the cuvettes. aFGF and
bFGF solutions with different concentrations were pipetted into the cuvettes and the progress of the interaction was monitored
in real-time by Windows-based software, yielding kinetic and equilibrium constants for these interactions. In addition, in
order to reduce the delicate difference among the cuvettes, inhibition analyses of mixtures of FGFs and immobilized native
heparin by modified heparins were also done. The data from these two methods were similar, indicating that all sulfate groups
at 2-O, 6-O and N- in heparin were required for the binding to aFGF; and that their contribution to the binding was in the
order 2-O, N- and 6-O-sulfate group. In contrast, definite contribution of the 6-O-sulfate group to the binding with bFGF
was most apparent, while the other two sulfate groups appeared to be necessary in the order 2-O and N-sulfate group. These
methods established here can be used for analysing the effect of sulfate groups in heparin on the binding with other human
FGF members or other heparin-binding proteins.
The project supported by the Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry
of China and Zhejiang Provincial Natural Science Foundation of China (301306) 相似文献
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黄芩苷片对缺血性大鼠脑组织中神经营养因子的影响 总被引：2，自引：0，他引：2
目的：观察黄芩苷片对缺血性大鼠不同时段脑源性神经营养因子（brain-derived neurotrophic factor,BDNF）、碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）、脑组织神经生长因子（nervegrowth factor,NGF）含量的影响,探讨黄芩苷片抗脑缺血损伤的机制。方法：参照Longa法复制脑缺血模型,用黄芩苷片干预,ELISA法测定脑组织BDNF、bFGF、NGF含量。结果：脑缺血12h和24h,黄芩苷片组脑组织BDNF含量均降低、bFGF含量增加;脑缺血12h和24h,模型组脑组织神经营养因子NGF含量反应性增加。结论：黄芩苷片在对脑缺血损伤神经元的保护作用中,能促进星形胶质细胞等产生bFGF。 相似文献
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