| 辣根过氧化物酶催化合成壳聚糖-没食子酸甲酯的条件研究 |
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| 引用本文： | 蓝华秀,薛丽群,陈盛.辣根过氧化物酶催化合成壳聚糖-没食子酸甲酯的条件研究[J].福建师大福清分校学报,2011(2):33-39. |
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| 作者姓名： | 蓝华秀 薛丽群 陈盛 |
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| 作者单位： | 福建师范大学化学与材料学院;福建师范大学福清分校; |
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| 摘 要： | 以辣根过氧化物酶（HRP）为催化剂,催化合成壳聚糖-没食子酸甲酯。探讨了接枝条件（酶用量、壳聚糖与底物浓度比例、底物浓度、反应时间等）对接枝率的影响,通过FTIR对其产物进行结构表征。结果表明,酶法催化合成适宜条件：辣根过氧化物酶（酶活力为40U/mL）用量为0.7mL,壳聚糖与没食子酸甲酯的量浓度比为3∶1,没食子酸甲酯浓度为7mmoL/L,反应时间2h。用线性电位滴定法测其接枝率为11.0%～37.2%。
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| 关 键 词： | 壳聚糖 没食子酸甲酯 辣根过氧化物酶 催化合成 |
Study on Conditions for Horseradish Peroxidase Catalyzed Synthesis of Chitosan-(methyl Gallate) |
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| LAN Huaxiu,XUE Liqun,CHEN Sheng.Study on Conditions for Horseradish Peroxidase Catalyzed Synthesis of Chitosan-(methyl Gallate)[J].Journal of Fuqing Branch of Fujian Normal University,2011(2):33-39. |
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| Authors: | LAN Huaxiu XUE Liqun CHEN Sheng |
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| Institution: | LAN Huaxiu1,XUE Liqun2,CHEN Sheng2 (1.Fujian Normal University,Fuzhou,350007 2.Fuqing Branch of Fujian Normal University,Fuqing,Fujian,350300) |
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| Abstract: | The enzymatic catalyzed grafting of methyl gallate onto chitosan was carried out by using horseradish peroxidase（HRP） as catalyst.The structure of the grating reactant was characterized by FTIR.The effect of reaction conditions on grafting ratio was studied,and the optimal conditions for grafting reaction were determined as follows：the amount of horseradish peroxidase（40U/mL）,0.7mL;ratio of chitosan to methyl gallate,3：1;methyl gallate concentration,7mol·L-1;reaction time,2.5h.The graft rate was determined to be 11.0-37.2% with linear titrimetric method. |
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| Keywords: | chitosan methyl gallate horseradish peroxidase catalyzed systhesis |
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