共查询到18条相似文献，搜索用时 439 毫秒
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固有无序蛋白是一类具有柔性结构的蛋白质,许多情况下通过与其它物质相互作用形成稳定结构来发挥重要生物功能.本文分别利用基于序列特征和结构特征的蛋白质结合位点预测程序对固有无序蛋白有序区和无序区与核酸分子的结合位点进行了预测分析.结果发现,基于结构特征的结合位点预测方法整体上要优于基于序列特征的预测方法,表明无序区尽管缺少稳定空间结构,结构特征依然在固有无序蛋白结合位点中发挥了重要作用.因此,可以为今后固有无序蛋白序列特征及结构特征的提取提供可靠依据. 相似文献
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钉螺血蓝蛋白的提取与性质研究 总被引：2，自引：0，他引：2
利用超高速冷冻离心和Sepharose 4B凝胶过滤两步法从日本血吸虫中间寄主-湖北钉螺血淋巴中提取获得一种含铜呼吸蛋白-钉螺血蓝蛋白.该血蓝蛋白具有典型的血蓝蛋白基本特征,含铜量为0.23%,在340nm处观测到该蛋白的紫外特征吸收峰.SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）检测该呼吸蛋白由两种蛋白质亚基组成,分子量分别为400KDa和250KDa.透射电子显微镜测定结果证实其四级结构为中凹的圆柱体,且以单体、二聚体和多聚体形式共存于血淋巴中. 相似文献
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固有无序蛋白质是一种新的蛋白质类型,具有显著的序列结构特征,尤其在与DNA相互作用中发挥着重要功能.分别统计分析了固有无序蛋白质有序区和无序区与DNA形成的复合物中,位于结合位点处脱氧核苷酸的结合偏好性以及与氨基酸组成结合对的偏好性.结果发现,参与这种相互作用的脱氧核苷酸类型具有显著差异,其中鸟嘌呤脱氧核苷酸和胸腺嘧啶脱氧核苷酸较受欢迎,而腺嘌呤脱氧核苷酸和胞嘧啶脱氧核苷酸不受欢迎.我们还发现四种脱氧核苷酸都偏好与精氨酸和赖氨酸组成结合对,而很少与天冬氨酸和谷氨酸结合.因此,本文将为今后DNA与其它分子相互作用研究提供可靠的数据和方法支持. 相似文献
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通过分析基因进化过程,以＂融合＂蛋白产生新功能或新蛋白质为实例,总结出蛋白融合的相关规则,规则包括协同作用、联合作用、多功能组合、多聚体组合和结构域重组等,根据这些规则设计了一个融合蛋白辅助筛选系统,用于融合蛋白规律的探讨以及为蛋白改造和设计提供辅助方案. 相似文献
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以新牧1号苜蓿为研究材料,采用RT-PCR方法,设计特异引物,从新牧1号苜蓿中克隆获得MvDREB基因cDNA,测序结果该片段全长为651 bp,Genbank登录号为GU073286.生物信息学分析结果表明,该基因序列与紫花苜蓿核苷酸序列相似度达99%,编码216个氨基酸,MvDREB蛋白氨基酸序列分析比对显示该蛋白属于AP2/ERF家族的亲水性核蛋白,蛋白质分子量为24873.1,理论等电点pI为5.90,不稳定参数为45.07. 相似文献
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花生致敏蛋白Ara h 1的电子克隆及生物信息学分析 总被引：1，自引：0，他引：1
花生致敏已成为重要的食品安全问题,花生蛋白中含有的Ara h 1是重要的致敏蛋白.运用生物信息学的电子克隆方法得到Shanyou523、Luhua14、USDA-Tifton三个花生品种的Ara h 1基因,并进行序列特征分析、多序列比对和结构域分析,探讨蛋白质水平的氨基酸差异与其致敏性的关系. 相似文献
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用功率谱的方法分析了不同类蛋白质氨基酸疏水序列 ,我们发现不同类蛋白质氨基酸疏水序列有着与其肽链结构中α -螺旋 ,β -折叠片顺序强烈相关的周期性 相似文献
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利用氨基酸理化性质中的等电点和疏水性,我们提出了一种新的蛋白质序列二维图形表示方法来刻画蛋白质序列.这种新的蛋白质序列图形表示方法可以有效地避免了图形曲线中可能出现的简并现象和图形任意性,而且蛋白质序列和它所对应的图形曲线是一一对应的. 相似文献
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In Swedish compulsory school, individual development plans (IDPs) are mandatory for all students up to 6th grade. The purpose is to summarize and facilitate pupils’ learning and tune instruction to national standards. In this study, 233 IDPs drawn up for 5th grade pupils were analyzed with focus on qualities that have been found to impact students’ learning and learner identities. The results show that the IDPs rarely display the qualities that would make them effective as tools for enhancing students’ learning, and that there is a gender difference in the quality of the documents, as well as differences regarding the pupils’ academic background. 相似文献
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Auditing, accountability, and transparency are concepts that greatly impact the working conditions of today’s public sector professionals, including teachers. Documentation requirements have been on the increase for some time, which can be seen in the education sector’s Individual Development Plans (IDPs), for example. These IDPs are pedagogical tools, but can also be seen as governance tools, which is how we will treat them here. In this conceptual paper, we argue that to improve knowledge of education policy changes, there is a need to consider the changed documentation requirements from the perspective of sociology of professions. To this end, we use Evetts’ occupational and organisational logics and Abbott’s three aspects of professional work. 相似文献
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Jennifer A. Hobin Philip S. Clifford Ben M. Dunn Susan Rich Louis B. Justement 《CBE life sciences education》2014,13(1):49-53
Individual development plans (IDPs) have been promoted nationally as a tool to help research trainees explore career opportunities and set career goals. Despite the interest in IDPs from a policy perspective, there is little information about how they have been used. The authors examined IDP awareness and use, the benefits of creating an IDP, and ways to facilitate its use by administering a survey to current or former postdoctoral researchers via the National Postdoctoral Association (NPA) and University of Alabama at Birmingham email lists; individuals belonging to Federation of American Societies for Experimental Biology member societies who mentored postdocs; and postdoctoral administrators at member institutions of the Association of American Medical Colleges and the NPA. Although most postdoctoral administrators (>80%) were familiar with IDPs, less than 50% of postdocs and only 20% of mentors were aware of IDPs. For those postdocs and mentors who reported creating an IDP, the process helped postdocs to identify the skills and abilities necessary for career success and facilitated communication between postdocs and their mentors. Despite the fact that creating an IDP benefits postdocs and mentors, IDP use will likely remain low unless institutions and research mentors encourage trainees to engage in this process. 相似文献
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利用GenBank数据库中Nodal分子的核酸和蛋白相关信息,采用生物信息学方法分析Nodal的分子生物学特征及其剪接异构体.应用基因克隆方法克隆人Nodal基因.结果表明Nodal基因至少有四种剪接异构体,除研究较清楚的isoform 1相对完整外,其它剪接异构体信息并不完整、功能未知且表达可能有组织特异性.特别是虽然Nodal基因isoform 1的氨基酸编码序列已知,但是发现目前NCBI提供的Nodal基因mRNA参考序列并不完整.通过基因克隆发现一种新的Nodal基因剪接异构体,它可能具有TGF-β超家族的特征性结构,但功能未知.结果说明人Nodal基因可能至少存在5种剪接异构体,除isoform 1外,其它isoform具体功能还有待进一步研究. 相似文献
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利用混沌效应的特性,提出基于混沌的密钥加密算法.混沌加密算法以非线性动力学中的混沌为理论基础,利用其初值敏感性和迭代不会重复性以产生序列随机数,从而应用于密钥数据加密,再把加密的密钥数据进行RSA加密.通过程序仿真表明,该方案能有效加密密钥数据,加密速度快,效果好,安全性强. 相似文献
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Kishore Dutta 《Resonance》2014,19(10):936-957
The dynamics of group chase-and-escape have attracted many physicists. In this article, we highlight the biological behaviors and the underlying mathematical rules and physical laws involved during the process of hunting in groups. We discuss some recently proposed stochastic models for such a system, which reveal various universal statistical features and show how slight variation of the behavioral rules or the interaction parameters can lead to very different statistical behaviors. A number of possible challenging questions that might lead to the development of a more life-like model for this novel enthralling dynamical problem are addressed. 相似文献
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Tong-bao LIU Guo-qing CHEN Hang MIN Fuc-heng LIN 《Journal of Zhejiang University. Science. B》2009,10(6)
Fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLP1, was isolated from a subtractive suppressive cDNA library and functionally analyzed. Sequence analysis showed that the MoFLP1 gene contains an open reading frame (ORF) of 1050 nucleotides encoding 349 amino acids with a calculated molecular weight of 35.85 kDa and a pl of 7.76. The deduced MoFLP1 protein contains a 17-amino acid secretion signal sequence and an 18-amino acid sequence with the characteristics of a glycosylphosphotidylinositol (GPI) anchor additional signal at its N- and C-terminuses, respectively. Potential N-glycosylation sites and domains involving cell adhesion were also identified in MoFLP1. Sequence analysis and subcellular localization by the expression of MoFLP1-GFP fusion construct in M. oryzae indicated that the MoFLP1 protein is probably localized on the vacuole membrane. Two MoFLP1 null mutants generated by targeted gene disruption exhibited marked reduction ofconidiation, conidiai adhesion, appressorium turgot, and pathogenicity. Our results indicate that fasciclin proteins play important roles in fungal development and pathogenicity in M. oryzae. 相似文献