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1.
A newly isolated strain EL31410, producing elastase (E.C3.4.4.7) with high elastolytic activity was identified as Bacillus sp. In the medium optimization, it was found that wheat bran and soybean flour hydrosate were the best crude carbon and nitrogen source for enzyme production, respectively. Addition of corn steep flour can affect the bacterium growth and elastase production. A fractional factorial design was applied to study the main factors that affect the enzyme production, and central composite experimental design and response surface methodology were adopted to derive a statistical model for the effect of wheat bran and soybean flour hydrosate on elastase production. The experimental results showed that wheat bran had positive effect but soybean flour hydrosate had negative effect, on enzyme production. An initial concentration of 3.4%(w/v) wheat bran and 9.4%(v/v) soybean flour hydrosate were found to be optimal for enzyme production in batch culture. The time course of elastase production in the optimized medium composition was also described. 相似文献
2.
A newly isolated strain EL31410, producing elastase (E. C3. 4. 4. 7) with high elastolytic activity was identified asBacillus sp. In the medium optimization, it was found that wheat bran and soybean flour hydrosate were the best crude carbon and nitrogen
source for enzyme production, respectively. Addition of corn steep flour can affect the bacterium growth and elastase production.
A fractional factorial design was applied to study the main factors that affect the enzyme production, and central composite
experimental design and response surface methodology were adopted to derive a statistical model for the effect of wheat bran
and soybean flour hydrosate on elastase production. The experimental results showed that wheat bran had positive effect but
soybean flour hydrosate had negative effect, on enzyme production. An initial concentration of 3.4% (w/v) wheat bran and 9.4%
(v/v) soybean flour hydrosate were found to be optimal for enzyme production in batch culture. The time course of elastase
production in the optimized medium composition was also described.
Project (No. 300024) supported by the Zhejiang Provincial Natural Science Foundation of China 相似文献
3.
Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis in shaking flask cul- tures. The optimal cultivation conditions stimulating the maximal elastase production consist of 220 r/min shaking speed, 25 h fermentation time, 5% (v/v) inoculums volume, 25 ml medium volume in 250 ml Erlenmeyer flask and 18 h seed age. Under the optimized conditions, the predicted maximal elastase activity was 495 U/ml. The application of response surface methodology resulted in a significant enhancement in elastase production. The effects of other factors such as elastin and the growth factor (corn steep flour) on elastase production and cell growth were also investigated in the current study. The elastin had no significant effect on enzyme-improved production. It is still not clear whether the elastin plays a role as a nitrogen source or not. Corn steep flour was verified to be the best and required factor for elastase production and cell growth by Bacillus licheniformis ZJUEL31410. 相似文献
4.
双孢蘑菇液体菌种培养基的优化 总被引：1，自引：0，他引：1
采用正交试验优化双孢蘑菇的液体培养基配方,发现黄豆粉、葡萄糖、硫酸镁、以及玉米粉和葡萄糖的交互作用对双孢蘑菇的液体培养有显著影响,磷酸二氢钾、麦麸汁对实验结果无显著影响。双孢蘑菇最佳液体培养基配方为:黄豆粉2%,玉米粉2%,葡萄糖2%,磷酸二氢钾0.05%,硫酸镁0.20%,麦麸0.20%。把双孢蘑菇液体菌种接入小麦栽培种中,菌丝表现出生长快的优势,生长速度较为一致,满瓶时间短,证明利用液体菌种制备栽培种比固体菌种具有更大的优势。 相似文献
5.
An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize the medium constituents. A central composite design was used to explain the combined effect of three medium constituents, viz, glucose, K2HPO4, MgSO4@7H2O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium. The fitted model of the second model, as per RSM,showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour 0.616 g/100 ml, K2HPO4 0.206 g/100 ml and MgSO4@7H2O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase production and cell growth. 相似文献
6.
INTRODUCTION Elastase is an enzyme that attacks and solubi-lizes elastin. As elastase can degrade elastin (Mori-hara, 1967) that other proteases cannot; it has broad applications in medical therapy, food processing and daily use chemicals industry. Considerable eff- orts were made to screen the elastase-producing strains, to study its pathogen effect and its charac-terizations (Tsuzuki and Oka, 1965; Tsai et al., 1988; Sharon et al., 1997; Ozaki and Shiio, 1975). Shiio, 1975). Reporte… 相似文献
7.
Xu YX Li YL Xu SC Liu Y Wang X Tang JW 《Journal of Zhejiang University. Science. B》2008,9(7):558-566
Objective: To study the optimal medium composition for xylanase production by Aspergillus niger XY-1 in solid-state fermentation (SSF). Methods: Statistical methodology including the Plackett-Burman design (PBD) and the central composite design (CCD) was employed to investigate the individual crucial component of the medium that significantly affected the enzyme yield. Results: Firstly, NaNO3, yeast extract, urea, Na2CO3, MgSO4, peptone and (NH4)2SO4 were screened as the significant factors positively affecting the xylanase production by PBD. Secondly, by valuating the nitrogen sources effect, urea was proved to be the most effective and economic nitrogen source for xylanase production and used for further optimization.Finally, the CCD and response surface methodology (RSM) were applied to determine the optimal concentration of each sig-nificant variable, which included urea, Na2CO3 and MgSO4. Subsequently a second-order polynomial was determined by mul-tiple regression analysis. The optimum values of the critical components for maximum xylanase production were obtained as follows: x1 (urea)=0.163 (41.63 g/L), x2 (Na2CO3)=-1.68 (2.64 g/L), x3 (MGSO4)=1.338 (10.68 g/L) and the predicted xylanase value was 14374.6 U/g dry substrate. Using the optimized condition, xylanase production by Aspergillus niger XY-1 after 48 h fermentation reached 14637 U/g dry substrate with wheat bran in the shake flask. Conclusion: By using PBD and CCD, we obtained the optimal composition for xylanase production by Aspergillus niger XY-1 in SSF, and the results of no additional expensive medium and shortened fermentation time for higher xylanase production show the potential for industrial utilization. 相似文献
8.
李豪 《商丘师范学院学报》2014,(6):51-54
以辅酶Q10产量为指标,对米曲霉2077固体发酵法生产辅酶Q10进行了初步系统性研究.利用配方均匀设计和正交设计方法分别对培养基和培养条件进行优化,建立了相应的数学模型,得到了较优固体培养基为大豆8.20%、麦麸63.53%和谷糠28.27%,确定100 g培养基固体培养条件为：料水比为1、青霉素钠加入量为3mL、发酵温度40℃、发酵时间96 h为较优工艺条件.在此优化培养基和培养条件下得到米曲霉2077产辅酶Q10的最大值为0.947/mg·g-1干培养基. 相似文献
9.
An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies
by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize
the medium constituents. A central composite design was used to explain the combined effect of three medium constituents,
viz, glucose, K2HPO4, MgSO4·7H2O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium.
The fitted model of the second model, as per RSM, showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour
0.616 g/100 ml, K2HPO4 0.206 g/100 ml and MgSO4·7H2O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found
that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase
production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which
can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase
production and cell growth.
Project (No. 300024) supported by the Natural Science Foundation of Zhejiang Province, China 相似文献
10.
Leticia I. RAMíREZ-CAVAZOS Charles JUNGHANNS Rakesh NAIR Diana L. CáRDENAS-CHáVEZ Carlos HERNáNDEZ-LUNA Spiros N. AGATHOS Roberto PARRA 《Journal of Zhejiang University. Science. B》2014,15(4):343-352
研究目的：优化获得血红密孔菌（P.sanguineus）的最佳培养基组成,提高耐热漆酶的产量。创新要点：获得了目前文献报道的最高水平的漆酶活力。研究方法：通过单因素试验研究了不同培养基（番茄汁、麦麸、麦芽提取物和葡萄糖细菌蛋白胨培养基）和不同组合诱导剂（大豆油、阿魏酸、没食子酸、二甲基苯胺、酸性蓝62和活性蓝19分别与硫酸铜组合诱导剂）对P.sanguineus产耐热漆酶的影响。在此基础上采用中心组合试验设计,进一步研究了番茄汁培养基结合硫酸铜和大豆油组合诱导剂对P.sanguineus产耐热漆酶的影响。利用SAS10.0和响应面分析方法对试验结果进行了统计分析和建立回归模型。重要结论：通过中心组合设计优化得出P.sanguineus产耐热漆酶的最优培养基条件：以36.8%番茄汁为培养基,以3 mmol/L硫酸铜和1%大豆油作为组合诱导剂。该条件下在10 L搅拌槽生物反应器中漆酶活力达到了143 000 IU/L（2,2＇-联氮双（3-乙基苯并噻唑啉-6-磺酸）为底物,pH值为3.0）。 相似文献
11.
黑皮鸡枞菌液体培养基优化 总被引：1，自引：0，他引：1
试验比较不同pH条件及培养基配方对黑皮鸡枞菌丝体（球）生长的影响以探讨最佳液体培养黑皮鸡枞最佳的pH及配方条件．通过预实验筛选出玉米粉、麸皮、大豆粉、K2HPO4、MgSO4为碳源、氮源及微量营养因子;用四因素四水平正交表进行试验,以菌丝体的干物质量为基准比较．结果表明：液体培养条件下,获得最高黑皮鸡枞菌丝体生物量的最佳配方为：麸皮14g／L·玉米粉11g／L·大豆粉2g／L·KH：PO4 1.2g／L·MgSO40．9g／L;最适pH为6．0． 相似文献
12.
通过正交交互设计试验优化灰树花的液体培养基配方,确定玉米粉和葡萄糖有交互作用,其比例为3∶2时为最优搭配。获得灰树花液体培养的最优配方为黄豆粉2%,玉米粉3%,葡萄糖2%,磷酸二氢钾0.05%,硫酸镁0.2%,豆油0.05%。将灰树花在50L发酵罐中进行深层培养,转速140r/min和通气量为300L/min,菌丝体产量为14.69g/L。 相似文献
13.
Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue （MIL-C） by Streptoverticillium rimofaciens ZJU 5119 总被引：1，自引：0，他引：1
Cytosine-substituted mildiomycin analogue (MIL-C) was produced effectively by supplementing cytosine into the culture of Streptoverticillium rimofaciens. In order to improve the yield of MIL-C, statistically-based experimental designs were applied to optimize the fermentation medium for S. rimofaciens ZJU 5119. Fifteen culture conditions were examined for their significances on MIL-C production using Plackett-Burrnan design. The Plackett-Burman design and one-variable-at-a-time design indicated that glucose and rice meal as the complex carbon sources, and peanut cake meal and NH4NO3 as the complex nitrogen sources were beneficial for MIL-C production in S. rimofaciens ZJU 5119. The results of further central composition design (CCD)showed that the optimal concentration of glucose, rice meal and peanut cake meal were 18.7 g/L, 64.8 g/L and 65.1 g/L,respectively. By using this optimal fermentation medium, the MIL-C concentration was increased up to 1336.5 mg/L, an approximate 3.8-fold improvement over the previous concentration (350.0 mg/L) with un-optimized medium. This work will be very helpful to the large-scale production of MIL-C in the future. 相似文献
14.
15.
Pleurotus sajor-caju (Fr.) Singer was cultivated on selected agro wastes viz. cotton stalks, groundnut haulms, soybean straw, pigeon pea stalks and leaves and wheat straw, alone or in combinations. Cotton stalks, pigeon pea stalks and wheat straw alone or in combination were found to be more suitable than groundnut haulms and soybean straw for the cultivation. Organic supplements such as groundnut oilseed cake, gram powder and rice bran not only affected growth parameters but also increased yields. Thus bioconversion of lignocellulosic biomass by P. sajor-caju offers a promising way to convert low quality biomass into an improved human food. 相似文献
16.
以饲料为原料筛选出一株既产乳酸又产芽孢的益生菌,经过分子生物学鉴定为凝结芽孢杆菌(Bacillus coagulans),命名为T-8。从培养基碳源、氮源、有机物浸出液、培养基pH、培养温度、培养时间等方面对菌株T-8展开发酵特性研究,结果表明,最优培养基配方为:玉米粉6 g/L、豆粕粉15 g/L、小麦麸皮浸出液60%、酵母粉5 g/L、蛋白胨10 g/L、氯化钠10 g/L,pH 5.0。以5%的接种量将种子液转接至最优培养基,在47℃的摇床中以200 r/min的转速振荡培养28 h,菌株T-8的细胞密度达到4.8×109 CFU/mL,芽孢率达到95.2%。本试验可为益生菌凝结芽孢杆菌应用于饲用微生物菌剂的工业化生产提供参考。 相似文献
17.
本研究用大豆和马玲薯替代部分面粉来生产面包,通过正交实验得出最佳的配料比是:大豆5%、马铃薯15%、糖12%.添加大豆和马铃薯的面包蛋白质、粗纤维和矿物质含量都有所提高. 相似文献
18.
Zinc (Zn) deficiency in animals became of interest until the 1950s. In this paper, progresses in researches on physi-ology of Zn deficiency in animals, phytate effect on bioavailability of Zn, and role of phytase in healing Zn deficiency of animals were reviewed. Several studies demonstrated that Zn is recycled via the pancreas; the problem of Zn deficiency was controlled by Zn homeostasis. The endogenous secretion of Zn is considered as an important factor influencing Zn deficiency, and the critical molar ratio is 10. Phytate (inositol hexaphosphate) constituted up to 90% of the organically bound phosphorus in seeds. Great improvement has been made in recent years on isolating and measuring phytate, and its structure is clear. Phytate is considered to reduce Zn bioavailability in animal. Phytase is the enzyme that hydrolyzes phytate and is present in yeast, rye bran, wheat bran, barley, triticale, and many bacteria and fungi. Zinc nutrition and bioavailability can be enhanced by addition of phytase to animal feeds. Therefore, using phytase as supplements, the most prevalent Zn deficiency in animals may be effectively corrected without the mining and smelting of several tons of zinc daily needed to correct this deficiency by fortification worldwide. 相似文献
19.
南韩灵芝母种培养基的筛选研究 总被引：1，自引：0，他引：1
通过对南韩灵芝菌株在不同碳源、氮源及不同配方的母种培养基的生长实验,筛选出适合其生长的碳源、氮源和最佳培养基配方．试验结果表明：南韩灵芝菌丝体在麸皮、酵母膏,蛋白胨3种氮源中,以麸皮为最适氮源;在葡萄糖、蔗糖和玉米粉3种碳源中,以玉米粉为最适碳源．在5种不同的培养基试验中,加富PDA培养基是最适合南韩灵芝生长的培养基配方． 相似文献
20.
WEI Pei-lian XU Zhi-nan CEN Pei-lin 《浙江大学学报(A卷英文版)》2007,8(9):1521-1526
Lovastatin production by Aspergillus terreus ATCC 20542 in solid-state fermentation (SSF) was studied. Various substrates were used to evaluate the ability of A. terreus to produce lovastatin. The results showed that either rice or wheat bran was suitable substrate for lovastatin production in SSF. The maximum yield of lovastatin (2.9 mg/g dry substrate) using rice as substrate was achieved after incubating for 11 d at the following optimized process parameters: 50%～60% initial moisture content,pH 5.5,incubation temperature 28 °C. 相似文献