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1.
INTRODUCTIONHypertensionisalwaysaccompaniedbyin creasesinarterywallthickness,mainlycausedbyproliferation ,hypertrophy ,migrationandap optosisofvascularsmoothmusclecells(VSMC) ,andelevatedcontentofconnectivetissue .Thesestructuralchangesinbloodvesselsarekn…  相似文献   

2.
Objective:The present study was designed to use an in vivo rabbit ear scar model to investigate the efficacy of systemic administration of endostatin in inhibiting scar formation.Methods:Eight male New Zealand white rabbits were randomly assigned to two groups.Scar model was established by making six full skin defect wounds in each ear.For the intervention group,intraperitoneal injection of endostatin was performed each day after the wound healed(about 15 d post wounding).For the control group,equal volume of saline was injected.Thickness of scars in each group was measured by sliding caliper and the scar microcirculatory perfusion was assessed by laser Doppler flowmetry on Days 15,21,28,and 35 post wounding.Rabbits were euthanatized and their scars were harvested for histological and proteomic analyses on Day 35 post wounding.Results:Macroscopically,scars of the control group were thicker than those of the intervention group.Significant differences between the two groups were observed on Days 21 and 35(p<0.05).Scar thickness,measured by scar elevation index(SEI) at Day 35 post wounding,was significantly reduced in the intervention group(1.09±0.19) compared with the controls(1.36±0.28).Microvessel density(MVD) observed in the intervention group(1.73±0.94) was significantly lower than that of the control group(5.63±1.78) on Day 35.The distribution of collagen fibers in scars treated with endostatin was relatively regular,while collagen fibers in untreated controls were thicker and showed disordered alignment.Western blot analysis showed that the expressions of type I collagen and Bcl-2 were depressed by injection of endostatin.Conclusions:Our results from the rabbit ear hypertrophic scar model indicate that systemic application of endostatin could inhibit local hypertrophic scar formation,possibly through reducing scar vascularization and angiogenesis.Our results indicated that endostatin may promote the apoptosis of endothelial cells and block their release of platelet-derived growth factor(PDGF) and fibroblast growth factor(FGF),thereby controlling collagen production by fibroblasts.Blood vessel-targeted treatment may be a promising strategy for scar therapy.  相似文献   

3.
探讨RhoC和基质金属蛋白酶-9(MMP-9)蛋白在结直肠癌发生发展中的作用。选取新鲜结直肠癌标本作为实验组,正常新鲜结肠粘膜组织作为对照组,应用流式细胞术分别检测RhoC和MMP-9的表达,检测其在不同临床病理特征之间的关系。结果表明,RhoC和MMP-9在结直肠癌组织中高表达(P<0.05),且其表达均与Dukes分期、浸润深度及炎细胞浸润相关(P<0.05),MMP-9的表达与结直肠癌的淋巴结转移密切相关(P<0.05),RhoC和MMP-9在结直肠癌中的表达呈正相关(r=0.3430,P=0.0420)。流式细胞术联合检测RhoC和MMP-9蛋白的表达有望成为判断预后的重要指标。  相似文献   

4.
目的:探讨两种家兔急性肾功能衰竭(ARF)模型的方法学差异.方法:皮下注射1%HgCl2(1.5ml/kg.bw,H组)、肌肉注射50%甘油(5.6ml/kg.bw、8ml/kg.bw、10ml/kg.bw三种剂量,依次为G1组、G2组、G3组)复制家兔ARF模型,测定24h和48h血清尿素氮(BUN)和肌酐(Cre)水平,观察比较各组模型复制的成功率、存活率及稳定性.结果:G1、G2两组不能成功复制ARF模型;G3组BUN、Cre较H组降低(P＜0.05).但G3组的存活率及稳定性均显著高于H组(P＜0.05).结论:两种模型复制方法各有优缺点,应根据不同研究目的选择相应的模型.  相似文献   

5.
Objective:The aim of this study is to investigate if dual-source computed tomography(DSCT) could guide the percutaneous coronary intervention(PCI) of chronic total occlusion(CTO).Methods:We enrolled patients who were confirmed to have at least one native coronary artery CTO by DSCT before they underwent selective PCI in the period from December 2007 to October 2008.A CTO was defined as an obstruction of a native coronary artery with no luminal continuity.The CT-guided PCI procedure involved placing CT and fluoroscopic images side-by-side on the screen.DSCT images were analyzed for location,segment,plaque characteristics,calcification,and proximal lumen diameter of the CTO before PCI.The guidewire was advanced and manipulated under CT guidance.The PCI was carried out and the results were compared.Results:Seventy-four CTOs were assessed.PCI was successful in 57 cases of CTOs(77.0%).According to the results,CTOs were divided into two groups:successful-PCI and failed-PCI.All coronary artery paths of CTOs were clearly recognized by DSCT.In the successful-PCI group,soft plaques were detected much more often than those in the failed-PCI group,but fibrous and calcified plaques were seen more often in the failed-PCI group.Calcification severity in CTO segments showed a significant difference between the groups(P=0.014).Calcified plaques were detected in 20(35.1%) lesions in the successful-PCI group.More than 70% of the failures were calcified plaques,of which there were two arc-calcified and one circular-calcified lesions.Occlusions were longer in the failed-PCI group than those in the successful-PCI group [(38.8±25.0) vs.(18.0±15.3) mm,respectively,P0.01].Fewer guidewires were used in the successful-PCI group compared with the failed-PCI group(1.7±1.0 vs.2.5±0.9,respectively,P0.01).The logistic regression analysis indicated that predictors of recanalization of CTOs included occlusion length(P=0.0035,risk ratio(RR)=0.93) and calcification severity(P=0.05,RR=0.27).Multi-linear trends analysis showed that the factors affecting procedural time were CTO location(P=0.0141) and occlusion length(P=0.0035).Conclusions:DSCT could delineate the path of CTOs and characterize plaques.The outcomes of PCI were related to thrombolysis in myocardial infarction(TIMI) flow grade,CTO characteristics,severity of calcified plaques,and the length of occlusive segments.Occlusion length and calcification severity were independent predictors of CTOs.Occlusion length and CTO segments could also help to estimate the duration of interventional procedures.  相似文献   

6.
选择产蛋率和体重相近的42周龄罗曼褐壳蛋鸡180只,随机分成6组,每组设5个重复,每个重复6只鸡,试验期8周.以基础日粮作为空白对照组,试验组分别在基础日粮中添加l%的陈皮、山楂、姜黄、决明子、大黄,以研究5种中草药对鸡蛋胆固醇的影响.结果表明,5种中草药都能降低血浆TG、TC、LDL水平和提高HDL含量,以山楂、决明子、大黄效果明显(P＜0.05);5种中草药都能降低鸡蛋胆固醇,但以山楂、决明子、大黄降低鸡蛋胆固醇作用显著(P＜0.05),与空白对照组相比,蛋黄胆固醇浓度分别降低14.83%(P＜0.05)、16.06%(P＜0.05)、13.84%(P＜0.05),鸡蛋胆固醇含量分别降低14.50%(P＜0.05)、15.56%(P＜0.05)、12.72%(P＜0.05).  相似文献   

7.
目的:观察甘油致急性肾功能衰竭(ARF)家兔自由基的变化,探讨自由基损伤在ARF发生中的作用及其意义.方法:30只家兔随机分为两组:50%甘油(10ml/kg.bw)肌肉注射复制家兔ARF模型,等量生理盐水代替甘油作为对照组(n均=15).分别应用改良TBA微量法、黄嘌呤氧化酶法测定血清及肾脏组织匀浆的丙二醛(MDA)水平、超氧化物歧化酶(SOD)活性的变化.结果:ARF组血清及肾脏组织匀浆的MDA水平高于对照组(P＜0.01),SOD活性显著低于对照组(P＜0.01).结论:自由基损伤在甘油致家兔ARF过程中具有重要作用.  相似文献   

8.
INTRODUCTION Matrine, a monomer of traditional Chinese medi-cine, comes from leguminosae plants such as Kusheng, is quinoilizidine with four-loop and molecular formula of C15H24N20. Matrine has been proved to have anti-arrhythmia (Xu et al., 2004), anti-hypoxia and decreasing heart rate effects (Zhang et al., 1990a; 1990b) in many animal experiments, and has the role of inducing calmness (Luo et al., 2001) and lowering body temperature (Tao and Wan, 1992). Traditional Chinese medicin…  相似文献   

9.
1日龄AA肉鸡160只,随机分成4组。1~4组饮水中肌肽添加剂量分别为0(对照组)、20(低剂量组)、40(中剂量组)、60(高剂量组)mg/L,试验期6周。于2、4、6周末每组随机取6只肉鸡心脏采血,测定血清中的谷丙转氨酶(ALT)、谷草转氨酶(AST)活力和总胆固醇(TC)的含量。结果表明:肌肽各剂量组ALT活力在2、4、6周龄时均低于对照组,但组间差异均不显著(P>0.05)。4周龄时各剂量组AST的活力均高于对照组,2、6周龄均低于对照组(P<0.05或P<0.01)。生长中期肌肽组血清胆固醇含量有所上升,生长后期略微下降,肌肽对肝脏功能没有明显的影响。  相似文献   

10.
目的：观察硝普钠与拉贝洛尔对高血压急症患者血液流变学的影响。方法：高血压急症56例,随机分为硝普钠组与拉贝洛尔组。28例采用硝普钠治疗,其它28例给予拉贝洛尔。两组均静脉持续用药6小时。每例于治疗开始和结束时分别进行血液流变学检测。结果：降压总有效率硝普组为100％（28／28）,拉贝洛尔组92．9％（26／28）,两组差异无统计学意义（P〉0．05）,但硝普钠组起效快于拉贝洛尔组。患者经硝普钠治疗后,血液流变学显示低切变率下全血粘度、高切变率下全血粘度、血浆比粘度、红细胞取集指数及血小板聚集率均显著下降（P〈0．01或P〈0．05）,与拉贝洛尔组比较,差异有显著性（P〈0．05）。拉贝洛尔组血液流变学各指标值虽有下降,但未达到统计学显著性程度（P〉0．05）。与药物有关的不良反应,硝普钠组明显低于拉贝洛尔组（3．6％,vs 21．4％,P〈0．05％）。结论：硝普钠治疗高血压急症疗效确切、起效快、不良反应少,且该药有改善血液流变性作用,优于拉贝洛尔。  相似文献   

11.
目的:探讨多肽对小鼠酒精性胃粘膜损伤的保护作用.方法:将45例小鼠分为对照组、模型组、保护组,用Guth方法分别计算3组损伤指数.结果:损伤指数模型组明显高于对照组(P＜0.05),保护组明显低于模型组(P＜0.05).结论:高浓度酒精可造成胃粘膜损伤,而多肽可降低酒精性胃粘膜损伤程度.  相似文献   

12.
Objective: To explore the frequency and significance of ApoE gene polymorphisms in Chinese patients with atherosclerotic cerebral infarction (ACI). Methods: Polymerase chain reaction and gene sequencing, single nucleotide polymorphisms of ApoE gene were used to analyze 33 cases of patients with ACI and 35 controls. Results: The frequencies of ApoE gene single nucleotide polymorphisms 465C/G, 462C/G and 451delC in the ACI group were significantly higher than those in the control group (P<0.05). The prevalence of polymorphism 486G/T in the control group was significantly higher than that in the ACI group (P=0.011). Conclusions: 465C/G,462C/G and 451delC polymorphisms might be associated with ACI.486GT allele might have protective effect on the pathogenesis of ACI.  相似文献   

13.
INTRODUCTIONAtheroscleroticCerebralInfarction (ACI)isoneofthemostcommoncerebralvasculardis easse.Itspathogenesishasnotyetbeencom pletelyexpounded ,althoughmoreandmorestudiesshowingthatgeneticfactorsmayplayanimportantrole ,especiallygeneticmutations.Lo cat…  相似文献   

14.
腹腔镜阑尾切除术与传统阑尾切除术疗效对比   总被引：2，自引：0，他引：2  
目的:比较腹腔镜阑尾切除术(laparoscopic appendectomy,LA)与传统剖腹阑尾切除术(open appendectomy,OA)的临床疗效。方法:将156例阑尾炎病例随机分成两组,一组72例行LA,另一组84例行OA,比较两种术式的手术时间、平均住院费用、住院时间,切口长度、术后镇痛剂应用、术后6h开始下床活动、术后24h内肛门排气例数、放置引流管率、切口感染等指标。结果:LA组的术后24h内肛门排气时间、放置引流管率、切口感染、平均住院时间、切口长度、粘连性肠梗阻、镇痛剂应用例数、术后6h开始下床活动例数与OA组比较,差异均有统计学意义(P<0.05);而两组手术时间比较差异无统计学意义(P>0.05);LA组住院费用较OA组明显增高(P<0.05)。结论:LA与OA比较,有手术损伤小、疼痛轻、恢复快、住院时间短、并发症少等优点,可作为大多数阑尾炎的首选手术方式。  相似文献   

15.
Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity, che-mopreventative and insecticidal properties. In this study, the toxic effects of L. elliptica essential oil against Sprague-Dawley rat's red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125, 250, and 500 mg/(kg body weight), respectively, and the control group received distilled water. Full blood count, RBC osmotic fragility, RBC morphological changes, and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb), mean cell hemoglobin concentration (MCHC), mean cell volume (MCV), and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05), the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinoeyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However, the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.  相似文献   

16.
目的:观察镍铬合金烤瓷冠修复后头发和血液中镍铬元素含量的变化,探讨镍铬合金长期存在于口腔中的安全性。方法:选取60名经体检合格的健康人作为正常对照组;60名上、下颌前牙有任意三颗行镍铬合金烤瓷冠修复的患者作为实验组。实验组修复后半年及一年和对照组均采用火焰原子吸收光谱法测定头发镍铬元素含量,采用石墨炉原子吸收光谱法测得其血清中镍铬元素含量。结果:头发镍含量:实验半年组与对照组比较无显著性差异(P>0.05);实验一年组与对照组相比较有显著性差异(P<0.05);实验一年组与半年组比较无显著性差异(P>0.05)。头发铬含量:各组之间比较均无显著性差异(P>0.05)。血清镍含量:实验半年组与对照组比较无显著性差异(P>0.05);实验一年组与对照组相比较有显著性差异(P<0.05);实验一年组与半年组比较无显著性差异(P>0.05)。血清铬含量:各组之间比较均无显著性差异(P>0.05)。结论:镍铬合金烤瓷冠修复后会在口腔环境中释放一定的镍、铬,且释放的量与时间有关,但量很微小,头发和血液中镍铬元素含量呈正相关。  相似文献   

17.
This study was aimed to investigate the relationship between breakfast and serum glucose, insulin and glucagon concentrations in order to establish a model breakfast appropriate for Chinese. Twenty-four volunteers were randomly assigned to four study groups: high carbohydrate breakfast, high fat and protein breakfast, the typical breakfast and fasting. Each subject had serum and urine samples collected while fasting and at 1,2 and 3.5 hours following the meal. The concentration of serum glucose, insulin and glucagon was measured. The levels of serum glucose in group A,B and C differed significantly at 1 and 2 hour after meal compared to those at fasting (P<0.05). The serum glucose in group A increased insignificantly after meal. The serum insulin levels were in group A,B and C significant different compared with control group(P<0.05).Those peaked at 1 hour after meal ,with group C rising the furthest. Compared with the fasting group, the serum glucagons rose and maintained the increase after breakfast in group A,B and C (P<0.05).The data suggested that various diets with different calorigenic amounts increased hormone concentration to various extents. We found that a breakfast rich in carbohydrates could maintain proper blood glucose level.  相似文献   

18.
目的:观察川芎嗪治疗实验性免疫性肾损伤作用和机理,为中药治疗肾小球肾炎开辟新途径。方法:猪血清腹腔注射8周,复制大鼠实验性肾损伤模型,川芎嗪治疗4周后处死动物,检测尿蛋白、血清BUN和Scr含量,取肾组织制作光、电镜标本检查。结果:川芎嗪大、小剂量组尿蛋白、血清BUN和Scr含量较模型组明显降低(P<0.01),肾小球的病理形态学改变较模型组轻。结论:川芎嗪具有治疗大鼠实验性免疫性肾损伤的作用。  相似文献   

19.
观察血清基质金属蛋白酶-9（MMP-9）及血管内皮生长因子（VEGF）在实验性新西兰白兔蛛网膜下腔出血后的浓度变化,探讨它们与脑血管痉挛发生、发展及转归过程的关系,为临床诊断及治疗提供理论依据。实验结果表明血清MMP-9及VEGF的浓度变化可以作为预测蛛网膜下腔出血后脑血管痉挛的发作、判断其发展及预后的参考指标之一,为临床诊断与治疗提供依据。MMP-9及VEGF参与了蛛网膜下腔出血后的病理生理过程,与迟发性脑血管痉挛的发病机制有密切关系。  相似文献   

20.
目的：探讨藻酸双脂钠（PSS）治疗急性脑梗死患者血液成分变化及疗效。方法：急性脑梗死患者158例，随机分为两组。对照组：78例，常规治疗；治疗组：80例，在常规治疗基础上，应用藻酸双脂钠注射液100mg加入0．9％氯化钠静脉滴注，每日一次，连续观察14d。对比分析二组治疗前后脂蛋白（a）、血脂、纤维蛋白原等血液流变学指标．并动态观察治疗前后临床神经功能缺损评分。结果：藻酸双脂钠可降低全血粘度、血浆粘度、血小板聚集率、纤维蛋白原及血清TC、TG、LDL，与治疗前比较有显著性改变（P〈0．01或P〈0．05）；与对照组比较有显著性差异（P〈0．01或P〈0．05）．但对脂蛋白（a）无影响；近期预后较常规治疗组好（P〈0．05）．结论：藻酸双脂钠可改善血液高粘滞状态，起到降低纤维蛋白原、抗血小板聚集作用，可改善近期临床神经功能缺损程度。  相似文献   

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