共查询到20条相似文献，搜索用时 234 毫秒
1.
目的:研究mi R-718和早期生长反应蛋白3(EGR3)在肝癌细胞系Hep G2及SMMC-7721中对细胞恶性行为的作用。创新点:首次在肝癌细胞系Hep G2和SMMC-7721中发现miR-718作为抑癌基因及EGR3作为促癌基因起到调控肿瘤恶性行为的作用。方法:采用聚合酶链反应(PCR)方法构建mi R-718和EGR3的过表达及敲降质粒,并采用实时定量PCR(q RT-PCR)方法验证质粒有效性;采用MTT法和克隆形成实验检测肝癌细胞系HepG 2和SMMC-7721的生长增殖能力;采用Transwell迁移侵袭实验检测肝癌细胞系Hep G2和SMMC-7721的迁移和侵袭能力;采用增强型绿色荧光蛋白(EGFP)荧光报告载体实验验证miR-718的靶基因;采用qR T-PCR和蛋白质印迹(Western blot)检测相关基因表达水平的变化。结论:mi R-718在肝癌细胞系HepG 2和SMMC-7721中起到抑癌基因的作用;EGR3在肝癌细胞系Hep G2和SMMC-7721中起到促癌基因的作用;miR-718是通过靶定EGR3 m RNA 3'UTR下调EGR3的表达起到抑癌基因的作用。 相似文献
2.
《学周刊C版》2017,(16):25-26
目的:观察探讨C35在肝癌中的表达与靶向干预,为临床治疗提供依据。方法:设计两对C35编码区的si RNA并合成,构建到转录载体p TZU6+1上,形成重组质粒siRNA-C35,在脂质体介导下转染肝癌细胞株,RT-PCR分析RNA干扰后mRNA的变化,Western blot检测表达蛋白的变化。结果:扩增的目的基因条带亮度存在明显差异,与对照组相比,转染质粒pTZU6+1相对表达率为95.3%、siRNA1组相对表达率为40.0%,siRNA2组的相对表达率为28.4%。构建的重组质粒对C35mRNA的表达有抑制作用。只转染脂质体组和pTZU6+1的C35蛋白杂交带强于siRNA1和siRNA2,证实重组质粒可以抑制C35基因的表达,以对照组的蛋白带为标准,其图像分析可知,转染质粒pTZU6+1相对表达率为94.9%、siRNA1组相对表达率为29.6%,siRNA2组的相对表达率为32.2%。结论:通过设计C35基因敲除的si RNA可有效抑制C35基因的表达,肝癌细胞生长速度及侵袭性均发生改变,但C35基因在肝癌细胞株中及肝癌组织中低表达,不可作为肝癌的靶点干预基因。 相似文献
3.
原发性肝癌（PHC）的发生是多因素、多阶段与多基因相互作用的结果。一般认为,细胞癌变和癌基因及相关基因调控与突变有关,所以任何一种致癌病因都发生在基因水平上。1早期研究50年代,大肝癌的诊断与切除依赖于死后诊断和对肝脏解剖生理生化的研究;60年代PHC的移植术取决于免疫学的发展;70年代,小肝癌的早期诊断和切除归功于甲胎蛋白（AFP）的应用;80年代,电脑技术应用于影像学而解决了PHC的定位诊断。近年来,PHC转移模型的建立与相关的癌基因、抑癌基因、生长因子、抗转移因子等的研究,为PHC的诊治奠定了理论基础。2动问… 相似文献
4.
5.
恶性肿瘤是威胁人类健康的重大疾病,其治疗的前提和关键在于找到恰当的靶点．survivin是表达于多种恶性肿瘤而不表达于终末分化组织的一种蛋白质,其功能主要在于抑制调亡和维持有丝分裂,在肿瘤的发生和进展中有关键性的作用．该蛋白的表达与肿瘤的治疗耐受性、预后不良和患者总体存活率下降相联系,干预其表达对肿瘤有重要的治疗意义．RNA干扰技术是进化上高度保守的基因组保护机制——基因组的免疫系统,是siRNA以碱基配对的方式在转录后水平特异性抑制基因表达,在感染及肿瘤等疾病的防治上有重大的应用价值．本研究拟以广泛表达的肿瘤抗原survivin为靶,设计特异的siRNA,构建表达质粒,选择胰腺癌和肺癌为模式肿瘤,将质粒转入细胞及荷瘤小鼠,观察该质粒在体外培养的肿瘤细胞系和荷瘤小鼠中抑制survivin的表达,以及预防、治疗肿瘤的效应,寻找高效特异的策略,为恶性肿瘤的生物治疗建立新的思路． 相似文献
6.
随着科学技术的飞速发展,对肿瘤生物学行为的研究日渐深入,目前,人们已逐渐认识到肿瘤的本质是细胞基因组本身的改变,特别是癌基因的激活、抑癌基因失活及其相互关系的改变,因此人们认为癌症是一种基因疾病。本文,对近年来人胃癌组织中癌基因和抑癌基因表达的研究现状作一简单综述。 1 癌基因的研究 1981年weinberg等首先报道人癌基因分离成功,肿瘤研究开始进入了癌基因时代。癌基因(oncogenes)又称致癌基因,它是能将正常细胞转化为癌细胞的DNA片断, 相似文献
7.
目的：探讨抑癌基因第10染色体同源丢失性磷酸酶一张力蛋白基因（PTEN）蛋白表达与子宫内膜癌的发生、发展的关系。方法：采用免疫组织化学S-P法检测PTEN蛋白在56例子宫内膜癌及30例正常子宫内膜组织的表达。结果：56例子宫内膜癌中PTEN蛋白表达缺失率为57．1％，明显高于正常子宫内膜中的完全表达（P〈0．05）。PTEN蛋白在低分化组的表达缺失率明显高于中高分化组（P〈0．05）。结论：抑癌基因PTEN蛋白表达缺失在子宫内膜癌的发生、发展、浸润等过程中有一定作用。 相似文献
8.
9.
用体外转录的方法制备得地高辛标记的cRNA探针,并以此对大肠癌组织及同一病人的正常粘膜进行SNC66基因表达的cRNA/mRNA原位杂交配对研究。结果表明,SNC66基因的表达细胞主要是上皮细胞和淋巴细胞;其在大肠癌中存在明显的表达缺陷。SNC66基因可作为候选抑癌基因加以研究、利用。 相似文献
10.
基因活动异常是细胞癌变的根本原因。在人类的染色体上 ,与癌变有关的基因有 2类 :一类称癌基因(ｏｎｃｏｇｅｎｅ) ,其表达可导致癌症的发生 ;另一类称抑癌基因 (ａｎｔｉｏｎｃｏｇｅｎｅ) ,其表达可抑制癌症的发生和发展。本文从基因、基因产物及其作用 3个环节介绍癌基因和抑癌基因对细胞癌变过程的调控作用。1 癌基因、癌基因产物及其作用目前 ,世界上已发现癌基因 1 0 0多个 ,其中一些存在于病毒中 ,称病毒癌基因 (ｖｉｒｕｓｏｎｃｏｇｅｎｅ) ;另一些则存在于细胞中 ,称细胞癌基因 (ｃｅｌｌｕｌａｒｏｎｃｏｇｅｎｅ)。人体细胞内… 相似文献
11.
目的 :研究螺旋CT双期增强扫描在胰头癌早期诊断和手术评估中的作用 .方法 :对2 0例胰头癌患者行螺旋CT双期增强扫描 ,扫描延迟时间分别为 4 0s和 70s ,对比剂用量 10 0ml,注射速度 2ml/s .比较肿瘤及正常胰腺CT值在两期中的变化 .根据CT征象对肿瘤可切除性进行评价 ,并与手术结果比较 .结果 :胰头癌在胰腺期以低密度影清晰显示 ,肝脏转移、淋巴结转移及胰周血管侵犯在门脉期清晰显示 .2 0例肿瘤中 ,术前认为可切除者为 12例 ,术中切除11例 .结论 :螺旋CT双期增强扫描能更清晰显示胰头癌有利于胰头癌的早期诊断和可切除性的判断 . 相似文献
12.
Shailendra Kapoor 《Journal of Zhejiang University. Science. B》2009,10(3):242-242
The article "Cationic liposome-mediated trans-fection of CD40 ligand gene inhibits hepatic tumor growth of hepatocellular carcinoma in mice" [doi:10. 1631/jzus.B0820178] by Jiang et a1.(2009) in a recent issue of the Journal of Zhejiang University SCIENCE B was highly thought provoking. The authors have clearly demonstrated the efficacy of CD40 ligand gene therapy in inhibiting the growth of hepatocellu-lar carcinomas. The findings of Jiang et al.(2009) are highly important as they further support and cor-roborate the rapidly expanding role of CD40 ligand gene therapy in the management of systemic malig-nancies besides hepatocellular carcinomas. 相似文献
13.
Liu Xuan Zhou Xue-qing Shang Xu-wei Wang Li Li Yi Yuan Hong Hu Fu-qiang 《Journal of Zhejiang University. Science. B》2020,21(3):218-233
Metastasis is one of the main reasons causing death in cancer patients. It was reported that chemotherapy might induce metastasis. In order to uncover the mechanism of chemotherapy-induced metastasis and find solutions to inhibit treatment-induced metastasis, the relationship between epithelial-mesenchymal transition (EMT) and doxorubicin (DOX) treatment was investigated and a redox-sensitive small interfering RNA (siRNA) delivery system was designed. DOX-related reactive oxygen species (ROS) were found to be responsible for the invasiveness of tumor cells in vitro, causing enhanced EMT and cytoskeleton reconstruction regulated by Ras-related C3 botulinum toxin substrate 1 (RAC1). In order to decrease RAC1, a redox-sensitive glycolipid drug delivery system (chitosan-ss-stearylamine conjugate (CSO-ss-SA)) was designed to carry siRNA, forming a gene delivery system (CSO-ss-SA/siRNA) down-regulating RAC1. CSO-ss-SA/siRNA exhibited an enhanced redox sensitivity compared to nonresponsive complexes in 10 mmol/L glutathione (GSH) and showed a significant safety. CSO-ss-SA/siRNA could effectively transmit siRNA into tumor cells, reducing the expression of RAC1 protein by 38.2% and decreasing the number of tumor-induced invasion cells by 42.5%. When combined with DOX, CSO-ss-SA/siRNA remarkably inhibited the chemotherapy-induced EMT in vivo and enhanced therapeutic efficiency. The present study indicates that RAC1 protein is a key regulator of chemotherapy-induced EMT and CSO-ss-SA/siRNA silencing RAC1 could efficiently decrease the tumor metastasis risk after chemotherapy.
相似文献14.
Ning SONG Yan WANG Xiao-dong GU Zong-you CHEN Liu-bin SHI 《Journal of Zhejiang University. Science. B》2013,14(6):451-459
Eukaryotic initiation factor subunit c(eIF3c) has been identified as an oncogene that is over-expressed in tumor cells and,therefore,is a potential therapeutic target for gene-based cancer treatment.This study was focused on investigating the effect of small interfering RNA(siRNA)-mediated eIF3c gene knockdown on colon cancer cell survival.The eIF3c gene was observed to be highly expressed in colon cancer cell models.The expression levels of the gene in eIF3c siRNA infected and control siRNA infected cells were compared via real-time polymerase chain reaction(PCR) and western blotting analysis.Cell proliferation levels were analyzed employing 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium bromide(MTT) and colony formation assays.Furthermore,the effects of eIF3c gene knockdown on the cell cycle and apoptosis were analyzed using flow cytometry.The results showed that suppression of eIF3c expression significantly(P<0.001) reduced cell proliferation and colony formation of RKO colon cancer cells.The cell cycle was arrested by decreasing the number of cells entering S phase.Further,apoptosis was induced as a result of eIF3c knockdown.Collectively,eIF3c deletion effectively reduced the survival of colon cancer cells and could be used as a therapeutic tool for colon cancer therapy. 相似文献
15.
Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression 总被引：3，自引：0，他引：3
Chen Z Xu ZF Ye JJ Yao HP Zheng S Ding JY 《Journal of Zhejiang University. Science. B》2005,6(4):236-241
INTRODUCTION Chronic infection by hepatitis B virus (HBV) isassociated with a high risk of liver cirrhosis and pri-mary hepatocellular carcinoma (HCC). It remains animportant global health problem with over 350 mil-lion chronic HBV carriers worldwide. Among thesechronic carriers, about one million people die ofHBV-associated liver failure or HCC annually (Kaoand Chen, 2002). Although chronically infected pa-tients have been treated with interferons and nucleo-side analogs, th… 相似文献
16.
孙永康 《湖州师范学院学报》2009,31(1)
为提高钡剂灌肠在大肠癌性梗阻诊断中的作用及X线影像表现,通过对16例大肠癌性梗阻经钡剂灌肠诊断为升结肠癌8例.横结肠近肝曲癌5例,直肠、乙状结肠癌3例的分析.得到结论:钡剂灌肠在大肠癌性梗阻急性发作病例中能及时有效地显示其病变部位梗阻性质和范围,可以弥补纤维结肠镜定位欠准确的不足. 相似文献
17.
目的探讨P53基因表达与胃癌、肠癌、食管癌的相互关系.方法采用SP免疫组化染色方法.检测了消化道癌中(胃癌22例、肠癌22例、食管癌60例)P53蛋白的表达率.结果P53蛋白在胃癌中表达率最高达63.6%,肠癌中表达率为55.0%,食管癌中的表达率为61.7%.结论P53蛋白在消化道肿瘤中普遍表达并且当癌浸润较深和有淋巴结转移者阳性率较高. 相似文献
18.
RNA干扰作用(RNAi)是通过双链RNA介导的特异性转录后基因沉默，这一过程已在拟南芥、线虫和真菌等多种模式生物中得到揭示．RNAi主要通过双链RNA被核酸酶切割成的小干扰RNA(siRNA)，由siRNA介导识别并靶向切割同源靶mRNA分子而实现的．RNAi具有高效性和高特异性，已成为关闭基因的一项新技术，在基因功能研究和疾病的基因治疗中具有广阔的应用前景． 相似文献
19.
INTRODUCTION In recent years, thermo-ablation of liver cancerhas received increasing attention. Generally this canbe achieved by radiofrequency (RF) or Nd:YAG laser(Tanabe et al., 2004; Nikfarjam and Christophi, 2003;Erce and Parks, 2003). Of great importance to themethod’s success is the size of the induced coagula-tion necrosis; the lesion size largely depends on thetemperature distribution in the target volume, whichis determined partly by the blood perfusion in thetarget or… 相似文献