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PSO312是从土壤分离的1个青霉菌株,生长中能产生不同酸碱性条件下显黄色与紫红色等天然色素物质．通过菌株固体平板上恒温培养,168h测定菌落直径的方法研究了温度、pH及碳、氮源对菌株（Penicilliumsp．）生长的影响．研究表明31℃为菌株生长的最适温度．31℃培养以pH2—3的最快,pH8—9生长次之,24℃培养以pH8—9生长最快,pH3—4其次．菌株以蛋白胨4g／L、牛肉膏4g／L、酵母膏2g／L及尿素2g／L为氮源培养的菌落直径分别为55．75mm、51．5mm、55．5mm及25．3mm．在（NH4）2SO4 2g／L、NH4NO3 3g／L、NaNO3 4g／L、KN034g／L用量之上培养生长最快,菌落直径分别为35mm、34mm、35mm、及32mm．葡萄糖、蔗糖用量30g／L、果糖20g／L及乳糖25g／L的生长速率最快,菌落直径分别为45mm、38mm、38mm及39mm．丙三醇与淀粉25g／L培养的菌落直径均为39mm． 相似文献
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黑曲霉A3木聚糖酶固态变温发酵及曲盘试验研究 总被引：2，自引：0，他引：2
黑曲霉A3是一株产木聚糖酶活力较高的菌株。本文研究了其在变温条件下的固态发酵产木聚糖酶情况,得出该菌利用麦秸、玉米芯和麸皮为原料．从33℃培养24小时后变温到28℃,可使酶活达到7200IU／g干培养基,产酶期比28℃恒温培养提前14小时,并在此基础上进行了曲盘扩大培养,酶活达到5118IU／g干培养基. 相似文献
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研究了凉州熏醋传统酿造大曲中细菌区系,对大曲中的细菌总数、醋酸菌、乳酸菌及耐热菌进行计数、分离纯化和鉴定,通过显微镜镜检、革兰氏染色、接触酶反应、需氧型、产酸产气、发酵性等特性测定,对分离的菌株进行了具体菌属鉴定.结果表明,大曲表面与大曲中心细菌区系差异明显;大曲中共分离到5株醋酸菌、8株乳酸菌和4株耐热菌;醋酸菌分属为醋杆菌属和葡糖杆菌属;乳酸菌分属为糖球菌属、链球菌属、魏斯氏菌属、乳球菌属和索丝菌属;耐热菌分属为微球菌属和芽孢杆菌属. 相似文献
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原油降解菌的筛选及其降解性能研究 总被引：1，自引：0，他引：1
从茂名炼油厂附近长期被石油污染的土壤中分离出2株能够以原油为惟一碳源和能源的原油降解菌株,经鉴定分属假单胞菌属和芽孢杆菌属。进一步研究了2株菌原油降解特性及其影响因素,结果表明：2株菌5d原油平均降解率分别为42．8％和44．7％;在pH接近7．0,NaCI的质量浓度为10g/L,表面活性剂吐温-80的质量浓度为40mg／L时降解效果最佳。 相似文献
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通过初筛、复筛,获得一株耐高温且产酸能力较强的菌株Y5,对该菌株进行了形态学和生理生化特性鉴定,确定此菌株为乳酸茵．通过单因素试验和正交试验确定菌株Y5的最佳产酸条件：发酵温度为55℃,转速为60r／min,接种量为7％,装液量为100mL． 相似文献
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除油菌的筛选及其处理采油废水的研究 总被引：1，自引：0，他引：1
通过对活性污泥的驯化,筛选处理采油废水的高效菌株。筛选得到三个属共5株高效菌株,除油能力在25．06～52．09％之间,COD去除能力在32．39～87．44％之间。混合菌生长迅速,没有延滞期,石油类最终去除率为94．34％,COD最终去除率为92．34％。高效细菌对石油类的去除率并不随着石油类含量的增加而上升。混合菌在石油类浓度为26．84mg／L时具有最高去除率96．64％;在COD浓度为491mg／L时具有最高去除率97．50％。混合菌株对芳香烃的去除能力最强,达到88．91％。对菌株进行紫外线辐照处理,获得3株诱变菌株,诱变菌株的除油能力以及除COD能力均有提高。 相似文献
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通过对Ｌ脯氨酸生产菌株—嗜醋酸棒杆菌（出发菌株为谷氨酸生产菌ＡＴＣＣ１３８７０）产酸机制及生理生化特点分析,掌握了该菌株高产Ｌ脯氨酸的相关条件,成功地在２０ｍ３发酵罐中将单批发酵产酸率、糖酸转化率从４３％和１７０％分别提高到６６％和２５０％,发酵周期缩短２０％ 相似文献
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在高初糖谷氨酸发酵中，高玉米浆用量、高生物素用量可以明显降低高初糖对菌体细胞的抑制作用；且在接种量为10％，玉米浆用量为0．55％，生物素用量为10μg／L，初糖为190g／L的谷氨酸发酵中，流加500g／L的浓糖，30h的产酸率达到145.8g／L，糖酸转化率达到60．32％。 相似文献
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建立了高效液相色谱法测定酱油和食醋中4种对羟基苯甲酸酯（对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸异丙酯、对羟基苯甲酸丙酯）含量的方法。紫外检测器254nm处测定。结果表明,4种对羟基苯甲酸酯在0.0～50.0mg/L范围内呈现良好线性关系,各组分的检出限分别为0.1mg/kg、0.2mg/kg、0.25mg/kg、0.2mg/kg（S/N=3）,不同剂量的加标回收率为86.3%～101.6%,相对标准偏差（RSDs）为0.7%～3.1%。该方法简便、快速、灵敏,准确度和重现性较好,可作为酱油和食醋中测定多种对羟基苯甲酸酯类添加剂的有效方法。 相似文献
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通过实验对尖孢镰刀菌NC03摇床发酵产脂肪酶的培养基组成和培养条件进行了优化,得出最佳产酶培养基组成配方为：蛋白胨5 g/L,酵母膏6 g/L,NaH2PO43 g/L;橄榄油250 mL/L,吐温80 25 mL/L。最优发酵条件为250 mL的摇瓶装液量50 mL,培养温度30℃,发酵时间84 h。经过优化后发酵液脂肪酶酶活力最高可达到11.32 U/mL,较优化前提高了3.0倍。 相似文献
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Studies on optimization of nitrogen sources for astaxanthin production by <Emphasis Type="Italic">Phaffia rhodozyma</Emphasis> 总被引：2，自引：0，他引：2
Ni H Chen QH Ruan H Yang YF Li LJ Wu GB Hu Y He GQ 《Journal of Zhejiang University. Science. B》2007,8(5):365-370
Fermentation ofPhaffia rhodozyma is a major method for producing astaxanthin, an important pigment with industrial and pharmaceutical application. To improve astaxanthin productivity, single factor and mixture design experiments were used to investigate the effects of nitrogen source on Phaffia rhodozyma cultivation and astaxanthin production. Results of single factor experiments showed nitrogen source could significantly affect P. rhodozyma cultivation with respect to carbon source utilization, yeast growth and astaxanthin accumulation. Further studies of mixture design experiments using （NH4）2SO4, KNO3 and beef extract as nitrogen sources indicated that the proportion of three nitrogen sources was very important to astaxanthin production. Validation experiments showed that the optimal nitrogen source was composed of 0.28 g/L （NH4）2SO4, 0.49 g/L KNO3 and 1.19 g/L beef extract. The kinetic characteristics of batch cultivation were investigated in a 5-L pH-stat fermentor. The maximum amount of biomass and highest astaxanthin yield in terms of volume and in terms of biomass were 7.71 mg/L and 1.00 mg/g, respectively. 相似文献
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INTRODUCTION The difficulties associated with large-scaleproduction of biotherapeutics provide a constantchallenge to the biotechnology industry. FDA hadadded “therapeutic DNA plasmid vectors” to the listof well-characterized biotechnology product (DoHHs,1996), and gene therapy has moved rapidly fromlaboratory scale to clinical trials. It is urgent to de-velop new protocols to obtain high-quality plasmidswith high yields and minimal or no contamination ofRNA and chromosomal D… 相似文献
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INTRODUCTION Butyric acid has several potential applications in industry. Its applications in the foodstuffs and beverage industries are widespread. It may be used as the pure acid in the dairy industry, or in the form of esters as a food additive to increase fruit fragrance. Furthermore, butyric acid could also have some important physiological functions. For example, butyric acid esters are the character-impact flavors in tropic fruits and dairy products (Centeno et al., 2002; Watson e… 相似文献
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The production of butyric acid by Clostridium butyricum ZJUCB at various pH values was investigated. In order to study the effect of pH on cell growth, butyric acid biosynthesis and reducing sugar consumption, different cultivation pH values ranging from 6.0 to 7.5 were evaluated in 5-L bioreactor. In controlled pH batch fermentation, the optimum pH for cell growth and butyric acid production was 6.5 with a cell yield of 3.65 g/L and butyric acid yield of 12.25 g/L. Based on these results, this study then compared batch and fed-batch fermentation of butyric acid production at pH 6.5. Maximum value （16.74 g/L） of butyric acid concentration was obtained in fed-batch fermentation compared to 12.25 g/L in batch fermentation. It was concluded that cultivation under fed-batch fermentation mode could enhance butyric acid production significantly （P〈0.01） by C. butyricum ZJUCB. 相似文献
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本文主要研究了稀土甘氨酸络合物 (简称MRG)的对柑桔生产的影响 .结果表明 :当用 1.2 3× 10 -3 mol·L-1MRG分别于花期和幼果期进行叶面喷施后 ,能促进柑桔的生长 ,改善柑桔果实的品质 ,提高果实着色面积和着色浓度 ,产量亦有所提高 相似文献
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Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue （MIL-C） by Streptoverticillium rimofaciens ZJU 5119 总被引：1，自引：0，他引：1
Cytosine-substituted mildiomycin analogue (MIL-C) was produced effectively by supplementing cytosine into the culture of Streptoverticillium rimofaciens. In order to improve the yield of MIL-C, statistically-based experimental designs were applied to optimize the fermentation medium for S. rimofaciens ZJU 5119. Fifteen culture conditions were examined for their significances on MIL-C production using Plackett-Burrnan design. The Plackett-Burman design and one-variable-at-a-time design indicated that glucose and rice meal as the complex carbon sources, and peanut cake meal and NH4NO3 as the complex nitrogen sources were beneficial for MIL-C production in S. rimofaciens ZJU 5119. The results of further central composition design (CCD)showed that the optimal concentration of glucose, rice meal and peanut cake meal were 18.7 g/L, 64.8 g/L and 65.1 g/L,respectively. By using this optimal fermentation medium, the MIL-C concentration was increased up to 1336.5 mg/L, an approximate 3.8-fold improvement over the previous concentration (350.0 mg/L) with un-optimized medium. This work will be very helpful to the large-scale production of MIL-C in the future. 相似文献
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林丽华 《鹭江职业大学学报》2014,(5):107-112
利用陶瓷膜和纳滤膜的组合分离技术代替板框对霉酚酸发酵液的过滤,对霉酚酸发酵液进行澄清和浓缩实验.采用陶瓷膜（0．1μm）和S-372纳滤膜技术对霉酚酸发酵液进行研究,研究了膜系统的通量、加水倍数和浓缩倍数等因素对膜分离性能的影响.在陶瓷膜加水4倍时,霉酚酸的收率可达96．5%,平均通量可达160-180 L/（ m2·h）;纳滤对霉酚酸的截留率和收率均达到98%,霉酚酸效价可浓缩到21 g/L,通量为25 L/（ m2·h）左右,与板框滤液效价相比,提高了3．5倍.结果表明：陶瓷膜和纳滤膜的组合工艺在产品收率及产品质量方面都明显优于传统的板框过滤,具有良好的应用潜力. 相似文献