共查询到18条相似文献，搜索用时 156 毫秒
1.
关于苯丙酮尿症的分析和治疗 总被引：1，自引：0，他引：1
苯丙酮尿症是一种常见的氨基酸代谢病,是由于苯丙氨酸代谢途径中的酶缺陷,使得苯丙氨酸不能转变成为酪氨酸,导致苯丙氨酸及其酮酸蓄积并从尿中大量排出。本病属常染色体隐形遗传,是先天代谢性疾病,也是为数不多的可治性遗传性代谢病。本文仅对本病的分析和治疗进行探讨。 相似文献
2.
利用甲酸脱氢酶(FDH)催化甲酸脱氢反应是酶促有机合成中重要的还原型烟酰胺辅酶再生系统。实验对E.coli BL21(DE3)/pET32/Bsp-FDH诱导表达NADP+依赖甲酸脱氢酶的表达条件进行了优化,在培养第4h加入0.4 mmol/l的IPTG、诱导时间8h、诱导温度30℃的诱导表达条件下,每升发酵液产甲酸脱氢酶170U。利用亲和层析法一步分离纯化重酶,纯化倍数为9.65、酶活回收达60.43%。实验为重组甲酸脱氢酶应用于有机合成中的NADPH再生奠定基础。 相似文献
3.
本文提出了一种通过D-苯丙氨酸的还原及环化合成2,2-二甲基-4-苯基恶唑酮的方法,经红外和核磁确定,目标产物正确。 相似文献
4.
猕猴桃猕猴桃是一种营养价值极高的水果,被誉为＂水果之王＂。它含有亮氨酸、苯丙氨酸、异亮氨酸、酪氨酸、丙氨酸等十多种氨基酸,以及丰富的矿物质,包括丰富的钙、磷、铁,还含有胡萝卜素和多种维生素。 相似文献
5.
达赉湖野生东北雅罗鱼LDH、MDH和SOD同工酶酶谱特征分析 总被引：1，自引：0，他引：1
本文采用聚丙烯酰胺凝胶电泳方法,分析了雅罗鱼的脑、肝、心、肌、眼等五种组织器官的乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)和超氧化岐化酶(SOD)同工酶的酶谱特征。结果表明:在不同组织中同工酶带各不相同,存在着较明显的组织特异性。 相似文献
6.
猕猴桃猕猴桃是一种营养价值极高的水果,被誉为"水果之王"。它含有亮氨酸、苯丙氨酸、异亮氨酸、酪氨酸、丙氨酸等十多种氨基酸,以及丰富的矿物质,包括丰富的钙、磷、铁,还含有胡萝卜素和多种维生素。猕猴桃对保持人体健康,防病 相似文献
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9.
分别以金华磐安、宁波章水和江苏南通的5个浙贝母品种为材料,研究了不同产地和品种对内在生理生化特性(如苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性及叶绿素含量等)的影响。结果表明5个品种中狭叶贝母的各项酶活性和叶绿素含量最高;其他品种酶活性和叶绿素含量各有差异,且不同部位活性也各不相同。从不同产地来看,磐安、章水、南通的贝母在不同酶活性和叶绿素含量上各有差异,其中在PAL、CAT、POD的活性上,章水贝母均要高于金华和南通贝母,而从PPO的活性和叶绿素的含量来看,则是金华贝母要高于章水和南通贝母。 相似文献
10.
《西藏科技》2020,(3)
目的探讨平原移居高原大鼠子代出生后第2个月内血清心肌酶受高原环境影响的变化。方法将8只(6只雌鼠,2只雄鼠) 8周龄wistar大鼠平均分为高原组和西洋参组,每组4只,按3:1雌雄比例合笼受孕,所有孕鼠自然分娩。比较高原组和西洋参组子代大鼠出生后的第2个月内血清心肌酶的差异。结果?高原组共有40只子鼠出生,西洋参组共有45只子鼠出生。高原组子代大鼠出生第7周乳酸脱氢酶、α-羟丁酸脱氢酶两项指标高于西洋参组(p 0. 05)。高原组子代大鼠出生第8周除乳酸脱氢酶、α-羟丁酸脱氢酶持续增高并高于西洋参组外(p 0. 05),肌酸激酶同工酶开始高于西洋参组(p 0. 05)。结论平原移居高原孕鼠子代血清心肌酶受高原环境影响而有变化。 相似文献
11.
采用二阶导数紫外光谱,研究了牛血清蛋白(BSA)中色氨酸(Trp)、酪氨酸(Tyr)和苯丙氨酸(Phe)3种芳香族氨基酸残基的精细吸收谱带,并结合其肽键的吸收谱带综合分析了BSA pH值在2.3~12区间的构象变化.研究结果表明,BSA在等电点(pH值 4.7)附近时构象变化较大;而在pH 值5.7~10区间,构象变化较小;在强酸(pH值2.3)和强碱(pH值12)环境中,BSA构象变化非常明显.同时还发现,BSA质量浓度也在一定程度上影响其构象. 相似文献
12.
D. S. Dhiman N. K. Sarin R. K. Sharma J. R. Kanwar R. M. Pathak R. L. Gupta 《Indian journal of clinical biochemistry : IJCB》1989,4(1):23-25
The present study has been conducted in 80 cases, out of which 28 were of strangulated and 52 of non-strangulated intestinal
obstruction. It has been observed that serum lactate dehydrogenase of more than 300 I. U./litre in first 24 hours to 72 hours
is highly suggestive of strangulated intestinal obstruction. 相似文献
13.
Role of plasma amino acids and gaba in alcoholic and non-alcoholic fatty liver disease-a pilot study
S. Mukherjee K. Vaidyanathan D. M. Vasudevan Subir Kumar Das 《Indian journal of clinical biochemistry : IJCB》2010,25(1):37-42
Alcohol appears to affect brain function, primarily by interfering with the action of gamma-aminobutyric acid (GABA) and other
neurotransmitters. As alcohol is mainly metabolized in the liver, therefore we undertook this pilot study to monitor the patterns
of changes in plasma amino-acid concentrations due to alcoholic and nonalcohol fatty liver disease and their relation with
plasma GABA level. Plasma amino-acid concentrations were measured in 25 alcoholic liver disease (ALD) patients, 18 non-alcoholic
fatty liver disease (NAFLD) patients, and 24 age and sex matched control subjects by HPLC. GABA concentration was elevated,
while isoleucine and leucine levels reduced significantly in ALD patients compared to the control subjects. Methionine and
phenylalanine levels elevated and valine content reduced significantly in ALD patients compared to other two groups, and GABA
level was significantly correlated with methionine and phenylalanine. Plasma concentration of lysine was significantly reduced
in both groups of liver disease patients compared to the control group, but was not correlated with GABA level. Glycine and
tyrosine levels reduced significantly in NAFLD patients compared to other two groups and were significantly correlated with
GABA. Interestingly, though amino acids such as alanine, histidine, proline and serine were not affected by liver diseases,
but were significantly correlated with GABA level. This pilot study indicated that alcoholic liver disease presented a more
deranged plasma amino acid pattern than nonalcoholic, and the amino acid imbalances. More studies are necessary to identify
the role of any particular amino acid on brain function and on neurotransmitter(s). 相似文献
14.
Singh TD Barbhuiya MA Gupta S Shrivastav BR Jalaj V Agarwal N Tiwari PK 《Indian journal of clinical biochemistry : IJCB》2011,26(2):146-153
We have studied the expression of lactate dehydrogenase and its isoforms in gall bladder cancer, cholelithiasis and chronic
cholecystitis. Quantitative and qualitative assays of lactate dehydrogenase and its various isoforms were carried out in the
blood sera of patients and healthy controls along with parallel estimation of various liver function test enzymes. Statistical
analysis was done using the software Graph Pad Prism. Significantly high expression of lactate dehydrogenase along with alkaline
phosphatase and total bilirubin (P ≤ 0.05) was observed in all the three clinical conditions as compared to controls. LDH showed an increasing trend from stage
I to stage IV of GBC indicating a significant positive association with the disease progression. The levels of LDH 3 and 4
isoforms appeared significantly more elevated in GBC than cholelithiasis or chronic cholecystitis. We suggest that a careful
estimation of total LDH and its isoforms 3 and 4 alone or along with alkaline phosphatase and total bilirubin during different
clinical stages, like chronic cholecystitis, cholelithiasis and GBC, may prove to be a potentially useful biomarker in the
prognostic management of gall bladder diseases, specifically GBC. 相似文献
15.
Biochemical activity of selenium and glutathione on country made liquor (CML) induced hepatic damage in rats 总被引：1，自引：0，他引：1
G. Kumar G. Sharmila Banu M. Rajasekara Pandian 《Indian journal of clinical biochemistry : IJCB》2007,22(1):105-108
The serum and hepatic enzymes of rats were studied after exposed to country made liquor (CML) along with two chelating agents
(glutathione and Selenium). There was a significant increase in several serum enzyme levels (viz., aspartate transaminase,
alanine transaminase, alkaline phosphatase, sorbitol dehydrogenase, glutamate dehydrogenase, bilirubin) and decrease in various
hepatic enzymes (Succinic dehydrogenase, Glucose 6-phosphatase, 5'Nucleotiease, Acid phosphatase, Acid ribonuclease, Cytochrome
P-450) due to repeated administration of CML (2ml/100g of body weight). Results of this study revealed that the GSH and Se
could give a significant protective action in serum and hepatic enzymes of CML exposed rats. 相似文献
16.
Shedid SM Saada HN Eltahawy NA Hammad AS 《Indian journal of clinical biochemistry : IJCB》2018,33(3):314-321
Radiation induced brain damage is associated with impairment of mitochondrial functions, variations in the level of neurotransmitters, and oxidative stress. Mitochondrial function is closely linked to the level of neurotransmitters since the precursors are supplied by the Kreb’s cycle intermediates. The objective of this study was to evaluate the influence of pantothenic acid, an essential component in the synthesis of Coenzyme A (CoA), on the activity of the Krebs cycle enzymes, isocitrate dehydrogenase (IDH), α-ketoglutarate dehydrogenase (α-KGDH), and succinate dehydrogenase (SDH); the level of aspartic, glutamic and GABA; the activity of transaminases, and oxidative stress, in the cerebrum of γ-irradiated rats. Pantothenic acid (26 mg/Kg) was orally administered to the rats, 2 h after irradiation and during the following 5 days. Animals were sacrificed the 7th day post-irradiation. The exposure of male albino rats to γ-rays (5 Gy) has triggered oxidative stress notified by a significant elevation in the level of malondialdehyde (MDA), an end product of lipid peroxidation, associated to a significant decrease in the content of phospholipids, and the antioxidant compound glutathione (GSH). The activity of IDH, α-KGDH, and SDH, has significantly decreased, while the level of aspartic, glutamic and GABA has significantly increased. In parallel to these changes, the activity of alanine and aspartate transaminase has significantly increased, compared to their values in the control rats. Pantothenic acid treatment, has significantly attenuated oxidative stress; enhanced the activity of IDH, α-KGDH, and SDH; minimized the increase in the level of amino acids and the activity of transaminases, compared to their values in the cerebrum of irradiated rats. In conclusion, pantothenic acid could improve the level of neurotransmitters amino acids, which depends on the enzymatic activities of Krebs cycle and linked to oxidative stress. 相似文献
17.
S. K. Bansal H. N. Pandey A. Bansal L. D. Joshi 《Indian journal of clinical biochemistry : IJCB》1992,7(2):179-184
Circadian rhythm of serum glucose, pyruvate, lactate and lactate dehydrogenase (LDH) was studied in normal healthy young volunteers
at the onset (I Group, 7 cases) and peak (II Group, 8 cases) of winters in India with highly varied temperature. There was
a significant difference, in the circadian rhythm of glucose, pyruvate and lactate dehydrogenase (LDH) of the two groups.
A clockwise shift was observed in acrophase except in serum lactate, which is related to the energy demand in association
with time qualified changes in diurnal activity of the individuals and change in photoperiod. 相似文献
18.
Uday Kumar Anand Sharan Shaheena Kamal 《Indian journal of clinical biochemistry : IJCB》2003,18(2):6-7
Usually CPK, CK-MB and lactate dehydrogenase (LDH) enzymes are measured in blood during the period of myocardial infarction.
The changes in LDH assay indicates duration of the infarction.
The level of lactate dehydrogenase is 500 times greater in tissue than those found in serum. A small mass of damaged tissue
causes leakage of enzyme and increases its level in serum.
Any cause of tissue break down/hemolysis which is sufficiently severe can produce LDH pattern similar to that in myocardial
infarction.
We report this case of small bowel volvulus showing significant increase in LDH.
It may be due to tissue necrosis which increases the level of LDH in serum. 相似文献