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《黑龙江科技信息》2016,(32)
目的:评价桑芪首乌片对正常大鼠的影响,为其临床应用提供试验依据。方法:SD雄性大鼠,50只,平均体重305.8g,随机分为5组,空白对照组、桑芪首乌片高剂量组(1.692g/ml)、中剂量组(0.846g/ml)、低剂量组(0.423g/ml)、卡托普利组(0.832g/ml),按照1ml/100g大鼠灌胃标准,依次给药,空白对照组给与等体积的溶媒(0.5%羧甲基纤维素钠溶液),连续灌胃15d后,1次/d,应用BL-420生物机能实验系统检测大鼠左侧颈总动脉血压。结果:桑芪首乌片0.423、0.846、1.6 92g/ml三个剂量组大鼠血压分别为83.89±8.26、93.42±4.1、102.44±10.17mm Hg,与空白对照组和卡托普利组相比,差异无统计学意义,P0.05。结论:桑芪首乌片对正常大鼠血压无影响。 相似文献
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目的:探讨中药复方海珠益肝化纤方对肝纤维化大鼠α-SMA和TGFβ1表达的影响。方法:将60只Wistar健康大鼠随机分为正常对照组、模型对照组、中药治疗组和阳性对照组,各15只。除正常对照组外,余下三组均采用四氯化碳(CCL4)造模8周制备肝纤维化模型。造模成功后,正常对照组和模型对照组给予0.9%生理盐水溶液,阳性对照组予秋水仙碱混悬液(0.25mg/kg/d),中药治疗组予海珠益肝化纤方混悬液(5m L/100g/d),连续用药治疗6周。观察各组大鼠肝功能(AST、ALT)、肝纤维化指标(HA、PCⅢ、IV-C、LN)、α-SMA和TGFβ1的变化。结果:与模型对照组相相比,阳性对照组、中药治疗组大鼠ALT、AST、HA、PCⅢ、IV-C、LN、α-SMA和TGFβ1水平下降(P0.05);与阳性对照组相比较,中药对照组大鼠ALT、AST、HA、PCⅢ、IV-C、LN、α-SMA和TGFβ1水平显著性降低,差异具有统计学意义(P0.05);海珠益肝化纤方组大鼠肝纤维化程度低于秋水仙碱组大鼠。结论:海珠益肝化纤方可降低CCL4诱导的肝纤维化大鼠α-SMA和TGFβ1的表达,抑制肝纤维化的进展。 相似文献
3.
观察中国林蛙卵油软胶囊的降血脂作用。将高脂造模成功的SD大鼠随机设3个剂量组和2个对照组,低、中、高剂量分别为0.17、0.50、0.83g/kg.BW/d林蛙卵油,相当于人体推荐摄入量的5倍、15倍和25倍。四周后取尾血,测定血脂各项指标。结果：与高脂对照组相比,中国林蛙卵油软胶囊实验组能明显降低高脂血症大鼠的TG含量,有显著性差异（P＜0.05）,高剂量组并能明显增高血清HDC-C含量,有显著性差异（P＜0.05）。结论：中国林蛙卵油软胶囊具有降血脂作用。 相似文献
4.
目的:研究四方藤60%乙醇提取物(EECH)对CII型胶原诱导类风湿关节炎模型(CIA)大鼠的抗风湿作用及其机制。方法:取40只Wistar大鼠随机分为正常对照组,模型组,阳性对照组,四方藤提取物高、低剂量组,每组8只。除正常对照组外,其余各组均采用CII胶原加氟氏完全佐剂法诱发类风湿性关节炎大鼠模型。EECH灌胃给予不同剂量提取物(2、0.5g.kg-1.d-1),模型组(雷公藤多苷片,1.5mg.kg-1.d-1),其余两组给予等量生理盐水,连续28天。测定大鼠血清炎症因子中TNF-α、IL-1β的表达,观察各组大鼠关节组织病理变化情况。结果:与模型组比较,EECH高、低剂量组可以显著降低CIA大鼠血清中炎症因子TNF-α、IL-1β的表达(P0.01)。病理检测表明,EECH可以显著改善CIA大鼠的关节病变。结论:EECH可对类风湿关节炎模型大鼠的关节具有明显的治疗作用,其机制可能与其下调血清中炎症因子中TNF-α、IL-1β的表达有关。 相似文献
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目的:观察大明胶囊对2型糖尿病大鼠心功能的作用,并探讨其作用机制。方法:用高脂饮食辅以链脲佐菌素(STZ)建立2型糖尿病大鼠模型,将空腹血糖值大于16.7mmol/L的大鼠随机分6组:糖尿病模型组、大明胶囊大剂量(200mg/kg/d)组、中剂量(100mg/kg/d)组、小剂量(50mg/kg/d)组、对照药罗格列酮(0.8mg/kg/d)组。各给药组连续灌胃给药30天后,HE染色和电镜观察大鼠心肌病理形态学改变;RT-PCR方法测定L型Ca2+通道α1cmRNA和K+通道KV4.2mRNA的表达。结果:大明胶囊可改善2型糖尿病所引起的LVSP、+dp/dtmax下降(P0.05),降低LVEDP、-dp/dt-max水平(P0.05),以中剂量作用最明显;形态学显示大明胶囊中剂量减轻了糖尿病大鼠心肌损伤;中剂量还可缩短注射SNP和PE后血压恢复时间以及增加NTS区c-fos免疫反应阳性细胞个数。结论:a.大明胶囊通过影响KV4.2和α1cmRNA的表达改善Q-T间期和P-R间期的延长;b.大明胶囊可通过改善糖尿病大鼠心肌结构的重塑提高2型糖尿病大鼠心脏收缩和舒张功能。 相似文献
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目的:观察祛银方联合雷公藤多甙片治疗寻常型银屑病临床疗效及安全性。方法:74例寻常型银屑病血热证患者随机分为祛银方联合雷公藤多甙片治疗组和雷公藤多甙片对照组。治疗4周后,评价治疗前后患者临床疗效、生活质量指数(DLQI)评分、中医临床症候症状等方面的改变,同时观察不良反应。结果:治疗后治疗组、对照组临床总有效率分别为87%、73%;两组生活质量指数(DLQI)均低于治疗前(P0.01),治疗后两组的DLQI评分有显著性差异(P0.01);治疗组中医症候均有显著性差异(P0.01);对照组除瘙痒症候治疗有显著性差异外(P0.01),余均无显著性差异(P0.05)。结论:祛银方联合雷公藤多甙片治疗寻常型银屑病具有良好的临床效果,尤其在改善中医症状如瘙痒、口渴等方面效果显著,能有效减轻雷公藤的毒副作用,起到减毒增效的作用。 相似文献
8.
目的:探讨甲氨蝶呤治疗类风湿性关节炎的应用及最佳剂量。方法:选择2017年4月至2018年2月90例类风湿性关节炎患者,随机分成2组。对照组给予雷公藤多苷片治疗,观察组则给予雷公藤多苷片联合小剂量的甲氨蝶呤治疗。比较两组疗效水平;关节肿胀、关节压痛、晨僵症状消失的时间、实验室指标正常时间;治疗前后患者关节肿胀指数、压痛指数、晨僵持续时间、休息痛评分、握力水平、C反应蛋白、血沉以及RF滴度;治疗不良反应。结果:观察组疗效水平高于对照组,P0.05;观察组关节肿胀、关节压痛、晨僵症状消失的时间、实验室指标正常时间优于对照组,P0.05;治疗前两组关节肿胀指数、压痛指数、晨僵持续时间、休息痛评分、握力水平、C反应蛋白、血沉以及RF滴度并无明显差异,P0.05;治疗后观察组关节肿胀指数、压痛指数、晨僵持续时间、休息痛评分、握力水平、C反应蛋白、血沉以及RF滴度优于对照组,P0.05。观察组治疗不良反应和对照组无明显差异,P0.05。结论:雷公藤多苷片联合小剂量的甲氨蝶呤治疗类风湿性关节炎的疗效确切,可有效改善值得推广应用。 相似文献
9.
目的:探究天然牛磺酸联合肝宁方治疗肝炎后肝硬化腹水的临床疗效。方法选取200例肝炎后肝硬化腹水患者,随机分为观察组和对照组,各100例。观察组采用天然牛磺酸联合肝宁方的方法治疗,对照组则采用单纯西医治疗。观察比较2组患者的临床治疗效果。结果:经治疗后:(1)观察组患者治疗效果的总有效率高达94.0%,显著高于对照组的79.0%(P0.05);(2)治疗后观察组ALB(44.72±2.09)g/L、ALT(57.25±21.09)U/L、TBLL(41.72±7.09)μmol/L,均优于对照组(40.18±1.06)g/L、(82.58±42.35)U/L、(47.18±15.06)μmol/L(P0.05)。结论:天然牛磺酸联合肝宁方治疗肝炎后肝硬化腹水临床疗效显著,临床上值得广泛推广使用。 相似文献
10.
本文以干燥的酸浆宿萼为实验材料,经乙醇回流提取,并通过溶剂萃取和大孔树脂进一步分离得到酸浆宿萼皂苷(TSP)。以不同剂量TPS连续20天对正常小鼠进行灌胃,探讨了TPS对小鼠免疫活性的作用。结果表明:高剂量(40mg/m L)的TPS溶液能极显著的增加小鼠的免疫器官指数(P0.05)和巨噬细胞吞噬指数;免疫球蛋白(Ig G)含量,P0.01;改善血常规指数。而低剂量(5mg/m L)的TPS溶液对小鼠上述指标有显著的正面影响,P0.05。总体上,在实验浓度范围内,TPS具有增强小鼠免疫活性的作用且呈剂量依赖性。 相似文献
11.
T. Malati G. Rajani Kumari B. Yadagiri 《Indian journal of clinical biochemistry : IJCB》2001,16(2):224-233
Ovarian cancer is the fifth leading cause of death in women. The incidence of this malignancy increases in women over the
age of 40. The overall five years survival is less than 30%, as most women present with advanced stage disease. Until recently,
detection of early stage ovarian cancer has been difficult since it is usually nonpalpable and asymptomatic. The definitive
diagnosis of an ovarian mass is a common problem in gynecologic patients with adnexal mass. The routine standard evaluation
for adnexal masses includes patient's history, physical examination, ultrasound and histopathological examination. These parameters
individually or in combination have little predictive value. The accuracy of diagnostic tools are of immense value and great
concern to practicsing Gynecologists and Oncologists. The clinical application of serum concentration of CA 125, AFP and hCG
is of great help not only as diagnostic aid but also in monitoring efficacy of any treatment modality like chemotherapy, radiotherapy
or surgical resection. Additionally, evaluation of tumor marker concentration helps in predicting early biochemical recurrence
and in prognostication in different types of ovarian malignancies. The ability to differentiate a malignant mass from a benign
pelvic mass pretherapeutically could be enhanced optimally by additional use of tumor markers such as cancer antigen CA-125,
alphafetoprotein and human chorionic gonadotrophin in pre-and postmenopausal women. 相似文献
12.
V. Thakur A. K. Anand U. Mukherjee D. Ghosh 《Indian journal of clinical biochemistry : IJCB》2003,18(2):27-33
Appropriate therapeutic measures can improve the life expectancy of patients with ovarian malignancy. There has been a pressing
need for serodiagnostic assays to enable, the close patient monitoring. Cancer Antigen 125 (CA125) has been described as a
useful marker in patient monitoring for ovarian malignancy. Keeping this in view, the present study was planned. 40 consecutive
female patients of ovarian carcinoma (mean age 52.4±10.7 years) were selected for serum CA125 analysis during the period of
year 1995–2001. The tumour marker concentration was compared with histologic types of ovarian tumour and the FIGO staging
of the disease. 25 healthy females (mean age 35.2–10.4 years) served as control. Mean serum CA125 concentrations in patients
with papillary serous adenocarcinoma(Mean±%CV 1571±121.5 U/ml) was much higher than patients with mucinous adenocarcinoma(775±78U/ml).
Mean serum CA125 concentration in endometrioid carcinoma was very high(2853±136 U/ml). The patient with clear cell carcinoma
however had shown moderate increase(60 U/ml). No correlation was found between serum CA125 concentration and the FIGO staging
of disease.
Quantitation of CA125 was most helpful in monitoring the response of treatment and followup of the patients after completion
of their treatment. Posttherapeutically its concentration showed more than 50% reduction in almost all (91.4%) patients (P<0.001).
Importantly these patients had also shown significant regression of the disease clinically and radiologically. 8.6% of patients
had shown static or increase in serum CA125 concentration which was associated with either clinically static or progressive
disease. Recurrence of the disease was noted in patients who had shown increase in serum CA125 concentration (biochemical
recurrence) in the followup
However, in our test population biochemical recurrence(increase in serum marker concentration) preceded the clinical or radiological
recurrence by an average of 6.5 months.
Kaplan meier survival analysis for evaluation of overall survival in our test subjects showed an overall survival of 32% at
one year and median survival of 9 months with confidence interval of 6.34 to 11.66. We conclude that serum CA125 is a useful
marker for monitoring the treatment and predicting an early recurrence of the disease in ovarian carcinoma patients. A study
in larger number of patients is needed to define its exact role in the management of the carcinoma ovary. 相似文献
13.
中西医治疗早发性卵巢功能不全研究进展 总被引：1，自引：0，他引：1
POI患者的卵巢功能及生育力低下是其就诊的主要原因,如何恢复POI患者的卵巢功能及提高生育力是研究重点。通过搜索知网、维普、万方等数据库近5年的文献资料,中西医均为早发性卵巢功能不全提供了新的研究方向。现就早发性卵巢功能不全的治疗进展进行综述。 相似文献
14.
In this study tumor markers CA 125, CEA and CA 19-9 were evaluated to assess the best tumor marker for epithelial ovarian carcinomas in diagnosis, prognosis and management. The results indicated that CA 125 is the best and the most superior marker today for the epithelial ovarian malignancies irrespective of the histologic type. CA 125 has the highest positivity rate in mucinous tumors compared to CEA and CA 19-9. Combined assay of either CEA or CA 19-9 or both along with CA 125 did not increase diagnostic sensitivity compared to sensitivity achieved by CA 125 alone for epithelial tumors of the ovary. Serum CA 125 concentration correlated well with pretherapy, stable course and recurrence of ovarian carcinoma. CA 125 could be used effectively for diagnosis, prognosis and monitoring of the ovarian malignancies as evident from long term follow up of adenocarcinoma ovary. 相似文献
15.
Sharmila Upadhya Subramanya Upadhya D. M. Vasudevan 《Indian journal of clinical biochemistry : IJCB》2003,18(1):46-51
The study was designed to evaluate the significance of tissue polypeptide specific antigen (TPS) in patients with histologically
proven ovarian and colorectal cancer following treatment along with CA125 (in ovarian cancer) and CEA (in colorectal cancer).
Patients were grouped as follows:
In patients with ovarian and colorectal cancer, the mean TPS levels were significantly higher in patients of group II compared
to group I. The percentage of patients above cut-off levels for TPS were 17.4% in group I and 95.5% in group II. Similar results
were observed with the mean levels of CA125. In colorectal cancer patients, the percentage of patients above cut-off levels
for CEA and TPS were 70% and 30% in group I and 100% in group II for both the markers. Our observations indicate that TPS
may be used as a common marker to indicate metastases in patients with ovarian and colorectal cancer. 相似文献
| Group I | : Patients with stable disease |
| Group II | : Patients with metastasis and relapse |
16.
P. Pragathi P. V. Bharath Kumar P. Amar Kumar M. Ramakanth Reddy V. Sravani J. Neeraja Eapen Reeba Mary K. Gopalakrishna 《Indian journal of clinical biochemistry : IJCB》2005,20(2):195-197
Adenosine deaminase (ADA) and 5′-nucleotidase (5′-NT) activities were measured in sera of patients with ovarian cancer and
patients with benign ovarian tumour. The results were compared with that of a control group consisting of healthy women. ADA
levels were significantly increased (P<0.001) in the ovarian cancer group (n=50) but not in the benign group (n=28) when compared
to the controls (n=20). The results indicate that ADA and 5′-NT levels may help to differentiate malignant conditions from
benign tumours of the ovary in addition to the existing tests such as serum CA-125 levels and histopathological study. 相似文献
17.
Salmanzadeh A Kittur H Sano MB C Roberts P Schmelz EM Davalos RV 《Biomicrofluidics》2012,6(2):24104-2410413
Ovarian cancer is the leading cause of death from gynecological malignancies in women. The primary challenge is the detection of the cancer at an early stage, since this drastically increases the survival rate. In this study we investigated the dielectrophoretic responses of progressive stages of mouse ovarian surface epithelial (MOSE) cells, as well as mouse fibroblast and macrophage cell lines, utilizing contactless dielectrophoresis (cDEP). cDEP is a relatively new cell manipulation technique that has addressed some of the challenges of conventional dielectrophoretic methods. To evaluate our microfluidic device performance, we computationally studied the effects of altering various geometrical parameters, such as the size and arrangement of insulating structures, on dielectrophoretic and drag forces. We found that the trapping voltage of MOSE cells increases as the cells progress from a non-tumorigenic, benign cell to a tumorigenic, malignant phenotype. Additionally, all MOSE cells display unique behavior compared to fibroblasts and macrophages, representing normal and inflammatory cells found in the peritoneal fluid. Based on these findings, we predict that cDEP can be utilized for isolation of ovarian cancer cells from peritoneal fluid as an early cancer detection tool. 相似文献
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P. K. Nigam A. Jain P. Goyal R. Chitra 《Indian journal of clinical biochemistry : IJCB》2005,20(2):43-47
Heat stable fraction (HSF) of alkaline phosphatase (ALP) was evaluated as an adjunct to CA 125 as a tumour marker for epithelial
ovarian cancer in a follow-up study. In our study group 63.4% of patients had elevated HSF levels (≥10U/L) and 93.3% had elevated
CA 125 levels (>35U/mL). The sensitivity of CA 125 and HSF was 93.3% and 63.3% respectively. The decline in the activity of
HSF, over the pre-op levels was highly significant after the first (p=0.001) chemotherapy cycle and significant after the
second and third cycles (p<0.029). Thereafter, HSF activity was almost undetectable. The decrease in CA 125 levels over the
pre-op levels was significant after the first, second, third (p<0.001) and fourth (p<0.034) chemotherapy cycle. HSF can be
used alone or as an adjunct to CA 125 in screening and monitoring patients of ovarian carcinoma especially in remote areas
where sophisticated facilities are not available and in patients in which CA 125 levels are not raised preoperatively. 相似文献
20.
目的分析西藏地区藏族2型糖尿病患者伴发血脂代谢异常情况,探讨血脂异常对糖尿病患者引起血管并发症方面的临床意义。对象2006年9月至2008年9月在本院住院的藏族2型糖尿病患者112例。方法所有患者根据有无合并血管病变分为两组,两组的血脂检测情况进行对比,同时与154例健康体检者进行比较。结果T2DM患者较对照组血脂显著异常,伴有血管合并症的T2DM患者较无血管合并症的T2DM患者血脂异常情况更明显。 相似文献