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1.
Objective: To investigate the evolution of pulmonary hypertension, the pathological changes of pulmonaryarteries, and the expression of VEGFmRNA and eNOSmRNA of pulmonary arterial endothelial cells in immature rabbitstreated with intratracheal bleomycin (BLM). Methods: Immature rabbits were divided into control and BLM group. Twoand four weeks after intratracheal normal saline or BLM injection, the systolic, diastolic and mean pulmonary arterypressure (PASP, PADP, MPAP) were measured by micro-catheter; the pathological changes and the expression ofVEGFmRNA and eNOSmRNA of endothelial cells in pulmonary arteries were evaluated by HE and in situ hybridization.Results: Two and four weeks after intratracheal injection of BLM, the PASP, PADP and MPAP increased 53%, 49%, 52%in 2 weeks, and 43%, 89%, 56% in 4 weeks; the wall thickness increased and the cavity in middle and small pulmonaryarteries became narrow; the Thickness Index (TI) and Area Index (AI) increased 25%, 14% in 2 weeks, and 22%, 24% in 4weeks; the level of VEGFmRNA and eNOSmRNA expression decreased 46%, 43% in 2 weeks, and 43%, 51% in 4 weeks.There was no significant difference between 2 weeks and 4 weeks BLM groups. Conclusion: The pulmonary artery pressurewas elevated, the thickness of wall increased and the cavity became narrow in middle and small pulmonary arteries, and thelevel of VEGFmRNA and eNOSmRNA expression in pulmonary arterial endothelial cells decreased in immature rabbitsafter 2 weeks and 4 weeks of intratracheal 4 U/kg BLM injection.  相似文献   

2.
INTRODUCTION Transformation, proliferation and migration of vascular smooth muscle cells (VSMCs) into en- domembrane of arteries are the pathological mani- festations of atherosclerosis. Gap junction (GJ) and connexin (Cx) situated between endothelial cells play an important role in this process. Currently, connexin between VSMCs that have been recognized at protein molecular level includes Cx40, Cx43, and Cx37 (Li and Simardm, 1999). It showed that the occurrence of arteriosclerosi…  相似文献   

3.
Objective: To study the relationships among magnetic resonance imaging (MRI), histological findings, and insulin-like growth factor-I (IGF-I) in steroid-induced osteonecrosis of the femoral head in rabbits. Methods: Thirty rabbits were randomly divided into experimental Group A (n=15) and control Group B (n=15). The 7.5 mg/kg (2 ml) ofdexamethasone (DEX)and physiological saline (2 ml) were injected into the fight gluteus medius muscle twice at one-week intervals in animals of Groups A and B, respectively. At 4, 8 and 16 weeks after obtaining an MRI, the rabbits were sacrificed and the femoral head from one side was removed for histological study of lacunae empty of osteocytes, subchondral vessels, and size of fat cells under microscopy, and the femoral head from the other side was removed for enzyme-linked immunoadsorbent assay (ELISA) for IGF-I.Results: At 4, 8 and 16 weeks after treatment, no necrotic lesions were detected in Group B, while they were detected in Group A.Light microscopy revealed that the fat cells of the marrow cavity were enlarged, subchondral vessels were evidently decreased,and empty bone lacunae were clearly increased. The IGF-I levels in Group A were significantly higher than those in Group B. At 8 weeks after the DEX injection, the MRI of all 20 femora showed an inhomogeneous, low signal intensity area in the femoral head,and at 16 weeks, the findings of all 10 femora showed a specific "line-like sign". The MRI findings of all femora in Group B were normal. Conclusion: MRI is a highly sensitive means of diagnosing early experimental osteonecrosis of the femoral head. However, the abnormal marrow tissues appeared later than 4 weeks when the expression of IGF-I increased. This reparative factor has an early and important role in response to steroid-induced osteonecrosis of the femoral head, and provides a theoretical foundation for understanding the pathology and designing new therapies.  相似文献   

4.
目的:研究复方金银花和茵陈五苓两种注射液对内毒素所致家兔肾衰中肾脏组织结构的保护作用。方法:将重量2.5±0.25kg的健康雄性家兔32只,随机分为对照组、模型组、复方金银花组和茵陈五苓组,每组8只。模型组、复方金银花组、茵陈五苓组家兔一次性静脉注射大肠杆菌O111B4内毒素生理盐水,剂量为100ug/kg。两保护组家兔注射内毒素24h后静脉注射剂量为100ug/kg的中药注射液。模型组家兔静脉注射同容积的生理盐水。对照组家兔静脉注射与内毒素溶液和中药注射剂等体积的生理盐水。静脉注射内毒素及生理盐水后第7h处死。取肾组织标本,Bouns液固定,石蜡切片,HE染色,显微观察并摄影。结果:模型组肾脏肾小体血管球肿胀,有血栓形成,肾小囊壁层内皮细胞脱落,肾小囊腔变小,肾小管内皮细胞脱落。复方金银花组、茵陈五苓组肾脏的肾小体血管球略有肿胀,肾小囊的内皮细胞较完整,肾小囊腔较对照组稍变小。结论:复方金银花与茵陈五苓对家兔因内毒素所致肾衰中肾脏组织结构有确实的保护作用。  相似文献   

5.
目的营养支持对慢性阻塞性肺部疾病患者血气和肺功能的影响。方法选择43例患者使用抗感染、平喘、化痰等常规治疗,并与43例进行有效营养支持的患者比较,治疗后1月血气分析以及肺功能的变化情况。结果治疗后观察组PO2和PCO2均达到正常范围,且PO2高于对照组（P〈0.05）,PCO2低于对照组（P〈0.05）,治疗后观察组患者FVC显著较对照组提高（P〈0.05）,且FEV1/FVC随之升高（P〈0.05）,同时FEV1%显著高于对照组（P〈0.05）。结论营养不良对于慢性阻塞性肺部疾病患者是一种疾病恶化的因素,进行有效的营养支持能改善患者血气分析结果,提高患者肺功能,值得临床重视。  相似文献   

6.
The aim of this study is to investigate the effects and possible mechanisms of sodium ferulate (SF) on anti-apoptosis in steroid-induced femoral head osteonecrosis in rabbits. Japanese white rabbits were randomly divided into three groups (control group, treatment group, and model group), each with 24 rabbits. The model and treatment groups were first injected with an intravenous dose of horse serum, 10 ml/kg, three weeks later with an intravenous dose of 7.5 ml/kg, and two weeks later with an intramuscular dose of methylprednisolone, 45 mg/kg, three times in order to establish rabbit models of osteonecrosis. Concurrently, the treatment group was injected with intravenous doses of SF 20 mg/kg for two weeks, once per day. Three time points, Weeks 2, 4, and 8, were selected after modeling was completed. Osteonecrosis was verified by histopathology with haematoxylin-eosin (HE) staining. The apoptosis rate of osteonecrosis was observed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The apoptosis expressions of caspase-3 and Bcl-2 were analyzed by immunohistochemistry and Western blot. The rabbit models of osteonecrosis were successfully established and observed by HE staining. SF was effective in intervening in apoptosis and decreasing the apoptosis rate in femoral head necrosis by the immunohistochemistry and TUNEL assay (P<0.01). Western blot analysis indicated that there were statistical significances in the protein levels of caspase-3 and Bcl-2 (P<0.01). SF has a protective effect by reducing the incidence of early steroid-induced femoral head necrosis in rabbits, effectively intervening in apoptosis through decreasing caspase-3 expression and up-regulating Bcl-2 expression.  相似文献   

7.
目的：观察急性肾功能衰竭（ARF）家兔心肌匀浆自由基、一氧化氮的变化，探讨心肌损伤的体液机制。方法：60只家兔均分为四组（n=15）。ARF模型1组：皮下注射1％HgCl2（1．3ml／kg．bw）；ARF模型2组：肌肉注射50％甘油（10ml／kg．bw）；以等量生理盐水代替HgCl2和甘油作为对照1、2组。24h后，所有动物颈总动脉放血备检，并选择固定位置。制备10％心肌匀浆。经Aeroset型全自动生化分析仪测定血清反映肾功能的生化指标。检测心肌匀浆丙二醛（MDA）、超氧化物歧化酶（SOD）、一氧化氮（NO）及其合酶（NOS）的变化。结果：与相应对照组比较，ARF模型1、2组心匀浆SOD活性下降、MDA含量升高（P〈0．05），ARF模型1、2组心匀浆NO含量增强、NOS及iNOS活性增强（P〈0．05～0．01）。结论：ARF家兔心肌损伤的机制与自由基损伤及NO升高有关。  相似文献   

8.
采用热缩聚法以L-天冬氨酸为原料合成了聚天冬氨酸（PASP）,并采用失重法与极化曲线法研究了在中性水体系中PASP对A3钢的缓蚀性能。实验结果表明,PASP对A3钢有较好的缓蚀作用,在该体系中是以抑制阳极为主的吸附型缓蚀剂,与抗坏血酸复配有协同作用,缓蚀效果最高达90%以上。  相似文献   

9.
ＩＮＴＲＯＤＵＣＴＩＯＮＤｏｘｏｒｕｂｉｃｉｎ(ＤＯＸ)ｉｓａｋｉｎｄｏｆａｎｔｈｒａｃｙ ｃｌｉｎｅｓａｎｔｉｂｉｏｔｉｃｓｗｈｏｓｅａｎｔｉｎｅｏｐｌａｓｔｉｃｓｐｅｃｔｒｕｍｉｓｂｒｏａｄａｎｄｅｆｆｅｃｔｉｓｓｔｒｏｎｇ.Ｉｔｈａｓｇａｉｎｅｄｗｉｄｅ ｓｐｒｅａｄｕｓｅｉｎｔｈｅｔｒｅａｔｍｅｎｔｏｆｃｈｉｌｄｈｏｏｄｌｅｕｋｅ ｍｉａａｎｄｓｏｌｉｄｔｕｍｏｒｓ.Ｈｏｗｅｖｅｒ,ｉｔｓｃｌｉｎｉｃａｌｕｓｅｉｓｌｉｍｉｔｅｄｂｙｉｔｓｃａｒｄｉｏｔｏｘｉｃｉｔｙ .Ｔｈｅｃａｌｃｉｕｍｏｖｅｒ…  相似文献   

10.
Objective: To investigate the directed transplantation of allograftic bone marrow-derived mesenchymal stem cells (MSCs) in myocardial infarcted (MI) model rabbits. Materials and Methods: Rabbits were divided into 3 groups, heart infarcted model with MSCs transplanted treatment (MSCs group, n=12), heart infarcted model with PBS injection (control group, n=20), sham operation with PBS injection (sham group, n=l 7). MSCs labelled by BrdUrd were injected into the MI area of the MSCs group. The same volume of PBS was injected into the MI area of the control group and sham group. The mortality, LVIDd, LVIDs and LVEF Of the two groups were compared 4 weeks later. Tropomyosin inhibitory component (Tn I) and BrdUrd immunohistochemistry identified the engrafted cells 4 weeks after transplantation. Result: The mortality of the MSCs group was 16.7% (2/12), and remarkably lower than the control group's mortality [35% (7/20) (P＜0.05)].Among the animals that survived for 4 weeks, the LVIDd and LVIDs of the MSCs group after operation were 1.17±0.21 cm and 0.74±0.13 cm, and remarkably lower than those of the model group, which were 1.64±0.14 cm and 1.19±0.12 cm (P＜0.05); the LVEF of the MSCs group after operation was 63±6%, and remarkably higher than that of the model group,which was 53±6% (P＜0.05). Among the 10 cases of animals that survived for 4 weeks in the MSCs group, in 8 cases (80%),the transplanted cells survived in the non MI, MI region and its periphery, and even farther away; part of them differentiated into cardiomyocytes; in 7 cases (70%), the transplanted cells participated in the formation of blood vessel tissue in the MI region. Conclusion: Transplanted allograftic MSCs can survive and differentiate into cardiomyocytes, form the blood vessels in the MI region. MSCs transplantation could improve the heart function after MI.  相似文献   

11.
目的总结43例恶性胸腔积液置管引流注药化疗的护理观察。方法采用Seldinger技术胸腔置管引流后,注入化疗药物,加强导管护理,注意临床观察。结果本组置管43例次,其中导管阻塞9例次（20.9%）,导管滑脱3例次（6.9%）,轻度穿刺点炎症4例次（9.3%）,未见导管断裂,近期胸腔积液完全清除15例（34.8%）,部分清除28例（65.1%）。结论加强对胸腔置管的护理及注意注药的方法是本组治疗的关键,此方法不良反应并发症少,患者痛苦少,治疗疗效佳,胸腔置管引流注入化疗药物是治疗恶性胸腔积液的最有效方法之一。  相似文献   

12.
目的观察川芎嗪酯类衍生物对H2O2引起的体外培养人脐静脉内皮细胞氧化性损伤的保护作用,并初步探讨川芎嗪双酯衍生物在家兔动脉内皮氧化损伤中的保护作用。方法H2O2引起体外培养人脐静脉内皮细胞及家兔动脉内皮氧化性损伤,观察川芎嗪双酯衍生物对内皮细胞氧化损伤的保护作用。结果在各试验浓度,大多数川芎嗪双酯衍生物对H2O2引起体外培养人脐静脉内皮细胞氧化性损伤有明显的保护及促增殖作用,A6在较高浓度对家兔动脉内皮的氧化损伤有保护作用。结论川芎嗪酯类衍生物对H2O2引起的体外培养人脐静脉内皮细胞氧化性损伤有明显保护作用,其中以A15效价最高,在其作用机制进一步研究中值得注意。  相似文献   

13.
Plexiform lesions (PLs), which are often accompanied by perivascular infiltrates of mononuclear cells, represent the hallmark lesions of pulmonary arteries in humans suffering from severe pulmonary arterial hypertension (PAH). Endothelial progenitor cells (EPCs) have been recently implicated in the formation of PLs in human patients. PLs rarely develop in rodent animal models of PAH but can develop spontaneously in broiler chickens. The aim of the present study was to confirm the presence of EPCs in the PLs in broilers. The immune mechanisms involved in EPC dysfunction were also evaluated. Lungs were collected from commercial broilers at 1 to 4 weeks of age. The right/total ventricle ratios indicated normal pulmonary arterial pressures for all sampled birds. Immunohistochemistry was performed to determine the expressions of EPC markers (CD133 and VEGFR-2) and proangiogenic molecule hepatocyte growth factor (HGF) in the lung samples. An EPC/lymphocyte co-culture system was used to investigate the functional changes of EPCs under the challenge of immune cells. PLs with different cellular composition were detected in the lungs of broilers regardless of age, and they were commonly surrounded by moderate to dense perivascular mononuclear cell infiltrates. Immunohistochemical analyses revealed the presence of CD133+ and VEGFR-2+ cells in PLs. These structures also exhibited a strong expression of HGF. Lymphocyte co-culture enhanced EPC apoptosis and completely blocked HGF-stimulated EPC survival and in vitro tube formation. Taken together, this work provides evidence for the involvement of EPCs in the development of PLs in broilers. It is suggested that the local immune cell infiltrate might serve as a contributor to EPC dysfunction by inducing EPC death and limiting their response to angiogenic stimuli. Broiler chickens may be valuable for investigating reversibility of plexogenic arteriopathy using genemodified inflammation-resistant EPCs.  相似文献   

14.
Objective： Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods： Healthy rabbits were randomly divided into four groups. Mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was per- formed with normal saline （group B）, vitamin C （group C） and vitamin E （group D）. Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline （sample B）, vitamin C （sample C） and vitamin E （sample D）. Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression. Results： In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes. Conclusion： Vitamin C can protect vascular endothelial cells from mannitol-induced injury.  相似文献   

15.
建立液态甲醛诱导小鼠肝损伤及其纤维化模型,并初步观察小鼠肝组织的病变过程．将正常昆明小鼠随机分为实验组和对照组．实验组小鼠注射15％的甲醛溶液,注射量为1mL／kg,每周注射两次,并且每天用1％的甲醛溶液喂养．对照组小鼠以纯净水正常喂养,分别在实验开始后的第3、6、8周处死实验组小鼠各4只,肉眼及病理切片观察其肝脏形态的改变,发现小鼠的体重均呈增长趋势．与实验组小鼠比较,对照组小鼠体重增长显著（P〈0．05）．第8周小鼠肝脏出现明显的病变,表面粗糙,颜色暗黄,出现深色斑点．在石蜡切片中可观察到肝脏组织变得松散,窦周隙（Disse’s space）增大．肝星状细胞（hepatic stellate cell,HSC）较对照组明显增多．随着甲醛处理时间的延长,HSC不同程度地增多,肝损伤病变程度越来越严重．  相似文献   

16.
INTRODUCTION Bronchioloalveolar carcinoma (BAC) is a par-ticular subtype of pulmonary adenocarcinoma de-rived from clara cell and type II pneumocyte. BAC cells grow along and within alveolar spaces while the alveolar framework of the lung is preserved. The incidence of BAC appears to be rising recently. The etiology and pathogenesis of this unique neoplastic disease are still unclear; many studies of oncogene and tumor suppressor gene expression include BAC with all adenocarcinoma o…  相似文献   

17.
INTRODUCTION Congestive heart failure is the end stage of manycardiovascular diseases. Myocardial infarction (MI)is a life-threatening event that may cause suddencardiac death and heart failure. Despite considerableadvances in diagnosis and treatment of heart disease,cardiac dysfunction after MI is still the majorworldwide cardiovascular disorder. Damaged myo-cardium after acute MI is gradually replaced by fi-brotic noncontractile cells to form scar tissue. Thedeveloping ventricul…  相似文献   

18.
Objective: To assess the effect of angiotensin Ⅱ type 1 (AT1) receptor antagonist losartan on myocardium connexin43 (Cx43) gap junction (GJ) expression in spontaneously hypertensive rats (SHRs) and investigate possible mechanisms. Methods: Sixteen 9-week-old male SHRs and 8 age-matched male Wistar-Kyoto (WKY) rats were included in this study. SHRs were randomly divided into two groups to receive losartan at 30mg/(kg·d) by oral gavage once daily for 8 weeks (SHR-L) or vehicle (0.9% saline) to act as controls (SHR-V); WKY rats receiving vehicle for 8 weeks served as normotensive controls. At the end of the experiment, rats were sacrificed and the hearts were removed. Expressions of Cx43 and nuclear factor-kappaB p65 (NF-κB p65) proteins in all three groups were observed and further investigations on the effect of angiotensin Ⅱ type 1 receptor antagonist losartan (30mg/(kg·d), 8 weeks) on Cx43 expression were conducted with Western blot and immunohistochemistry. NF-κB p65 protein in nuclear extracts was determined by Western blot. Results: Left ventricular (LV) hypertrophy was prominent in SHRs, Cx43 and NF-κB p65 protein expressions were obviously upregulated and Cx43 distribution was dispersed over the cell surface. Treatment with losarton reduced the over-expressions of Cx43 and NF-κB p65 in LV myocardium. The distribution of Cx43 gap junction also became much regular and confined to intercalated disk after losartan treatment. Conclusion: Cx43 level was upregulated in LV myocardium of SHR during early stage of hypertrophy. Angiotensin Ⅱ type l receptor antagonist losartan prevented Cx43 gap junction remodeling in hypertrophied left ventricles, possibly through the NF-κB pathway.  相似文献   

19.
目的：探讨NF-кB在肝癌介入治疗后肿瘤组织和癌旁肝细胞中的表达及其变化特点。方法：建立VX2肝癌模型兔59只,分为A组（肝癌组）29只,B组（肝癌经导管肝动脉栓塞介入组）30只、C组（对照组）20只,免疫组化检测各组肝癌及癌旁肝组织中的NF-кB表达。结果：A组肝癌组织与癌旁肝组织NF-κB阳性表达率分别为72.4%（21/29）、37.9%（11/29）,肝癌介入治疗后肝癌组织和癌旁组织NF-κB阳性表达率82.4%（24/30）、60%（18/30）,C组NF-κB阳性表达率为10%（2/20）,各组与对照比较,除A组癌旁肝组织外差异均有统计学意义（P〈0.05）,其余两两比较差异无统计学意义（P〉0.05）。结论：治疗前癌旁组织,其内NF-кB的表达水平尚处于较低水平,介入治疗后的癌旁肝组织的脂肪变性及炎性反应等因素可能会导致NF-κB的表达增加。  相似文献   

20.
Background: An ideal aneurysm model of cerebral aneurysm is of great importance for studying the pathogenesis of the lesion and testing new techniques for diagnosis and treatment. Several models have been created in rabbits and are now widely used in experimental studies; however, every model has certain intrinsic limitations. Here we report the development of a novel saccular aneurysm model in rabbits using an arterial pouch that is subject to in vitro pre-digestion with combined elastase and collagenase. Methods: A segment of right common carotid artery (CCA) was dissected out and treated with elastase (60 U/ml, 20 min) followed by type I collagenase (1 mg/ml, 15 min) in vitro. The graft was anastomosed to an arterial arch built with the left CCA and the remaining right CCA, while the other end of the graft was ligated. The dimension and tissue structure of the pouch were analysed immediately, 2 or 8 weeks after operation. Findings: Ten terminal aneurysms were produced. The gross mor-phology of the aneurysm resembles the human cerebral terminal aneurysms. We have observed the following pathological changes: (1) growth of the aneurysm (mean diameter increased from (2.0±0.1) to (3.2±0.3) mm at 2 weeks, P<0.001, n=7~10); (2) thinning of the aneurysmal wall (the mean wall thickness decreased to 44% at 2 weeks), which was accompanied by significant losses of elastic fibres, collagen and the cellular component; and (3) spontaneous rupture (3 out of 9, one aneurysm ruptured 24 h after operation with the other two at 2 and 4 weeks respectively). Conclusion: This rabbit arterial pouch model mimics human cerebral aneurysms in relation to morphology and histology. In particular, this model exhibited an increased tendency of spontaneous rupture.  相似文献   

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