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1.
目的:研究匙羹藤总皂苷对大鼠肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、和脂质过氧化物(LPO)作用的影响。方法:通过硫代巴比妥酸(TBA)分光光度法测定大鼠肝脏脂质过氧化(LPO)的二级分解产物丙二醛(MDA)的含量,以考察匙羹藤总皂苷对实验大鼠肝脏LPO的作用影响,采用黄嘌呤氧化酶法测定SOD活力,钼酸铵显色法测定CAT的活力,考察匙羹藤总皂苷对实验大鼠肝脏SOD和CAT的作用大小。结果:与对照组比较匙羹藤总皂苷在1.0mg/ml~6.0mg/ml浓度范围可抑制大鼠肝脏LPO作用;在1.0mg/ml~6.5mg/ml浓度范围内可提高大鼠肝脏SOD和CAT的活性。结论:匙羹藤总皂苷可以提高大鼠肝脏SOD和CAT的活性,降低MDA的产生。  相似文献   

2.
目的:通过复制D-半乳糖所致的衰老小鼠模型,探讨广西产五指毛桃不同提取物的抗衰老作用。方法:将70只实验小鼠随机分为7组:正常组,模型组,维生素E(Vit E)组(100mg/g),五指毛桃水提物高(20g/kg)、低(10g/kg)剂量组,五指毛桃醇提物高(20g/kg)、低(10g/kg)剂量组,在给药30d后,计算小鼠胸腺及脾脏指数,检测小鼠血清MDA含量及SOD活力、肝组织GSH-Px活力、脑组织CAT活力。结果:五指毛桃可显著增大衰老小鼠胸腺、脾脏指数,并不同程度提高衰老小鼠血清SOD活力、肝组织GSH-Px活力、脑组织CAT活力,降低小鼠血清MDA水平。结论:广西产五指毛桃具有一定抗衰老作用,其机制可能与提高机体免疫和抗氧化能力有关。  相似文献   

3.
研究了不同药剂处理对低温逆境下不同耐寒力烟草品种种子发芽和幼苗生长的影响。采用不同浓度的外源脯氨酸、水杨酸、氯化钙、甘油、二甲基亚砜、乙二醇、聚乙二醇、丙二醇、烯效唑、脱落酸对不同耐寒力烟草品种种子浸种处理后,测定了低温逆境下不同烟草品种发芽及幼苗生长和生理生化指标变化。结果表明,脯氨酸、水杨酸、氯化钙、二甲基亚砜和聚乙二醇5种药剂浸种能显著促进烟草种子发芽,提高了低温逆境下幼苗根长、苗高、幼苗干鲜重以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,其中10 g/L脯氨酸、120 mg/L水杨酸、15 mg/L氯化钙、30 ml/L二甲基亚砜和100 ml/L聚乙二醇综合效果较好,可以作为提高烟草种子及幼苗抗寒性的处理方法。  相似文献   

4.
香根草提取物对二化螟生长发育及体内保护酶活力的影响   总被引：1，自引：0，他引：1  
将香根草地上部分不同极性溶剂(石油醚、乙酸乙酯、正丁醇和水)的提取物加入到人工饲料中喂食二化螟幼虫,进行了提取物对二化螟初孵幼虫的毒杀作用、三龄幼虫的生长发育抑制作用及其体内保护酶(POD,SOD和CAT)活性影响的测定分析。结果表明,不同溶剂的提取物(0.01 g/m L)对二化螟幼虫均有毒杀作用,其作用大小排序为石油醚乙酸乙酯正丁醇水,其中石油醚提取物处理3 d后二化螟初孵幼虫存活率为15.8%,5 d后存活率为0.8%。三龄幼虫取食添加了不同提取物(0.05 g/ml)的人工饲料7 d后,生长发育均得到明显抑制,以石油醚和乙酸乙酯提取物作用最强,生长发育抑制率分别达111.11%和109.50%;同时,三龄幼虫体内的SOD活力明显高于对照,CAT和POD活力明显低于对照,其中石油醚和乙酸乙酯提取物和对照差异均达显著水平。研究表明香根草中含对二化螟幼虫有毒杀和生长发育抑制作用的活性物质,以石油醚和乙酸乙酯提取物中最为丰富。香根草提取物扰乱了二化螟幼虫体内保护酶SOD、CAT和POD的动态平衡,使虫体内自由基的清除遇到障碍,从而对其产生毒害作用。  相似文献   

5.
以分光光度法对三种水生植物,普生轮藻、豆瓣菜和菹草在优化条件下吸附苯后叶绿素、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的变化进行了研究.结果表明,处理组与对照组相比,叶绿素含量和SOD活性无明显变化或略有升高,CAT活性有不同程度的升高.三种植物对苯具有良好的适应性和耐受性.  相似文献   

6.
目的：观察不同磁感应强度的恒定强磁场对家兔血清中矿物质与微量无素的代谢影响。方法：将20只家兔随机分为实验组与对照组。结果发现：1.成年家兔于50mT或100mT磁场暴露48h后,血清钠含量较对照组少,锌、铜、锰,铁未见有显著变化。2．幼年家兔于10mT磁场暴露8周;于20mT磁场暴露8周后,血清矿物质与微量元素含量,除锌、铜有变化外其它未见有明显变化。  相似文献   

7.
目的:观察不同运动方式对小鼠肝脏线粒体GSH-Px的影响,筛选出有效促进机体氧化抗氧化平衡的运动方式提供理论依据。方法:选用纯系雄性C57BL/6J小鼠32只作为研究对象,并随机分为四组,经过运动训练后,GSH-Px的活性;采用实时荧光定量PCR法检测肝脏线粒体中GSH-PxmRNA表达的变化。结果:耐力组GSH-Px mRNA的表达(P0.05);抗阻组(P0.01);耐力训练组与抗阻训练组比较(P0.05)。与对照组相比,耐力组同抗阻或混合组与抗阻组比较(P0.05)。结论:在三种运动方式中效果最为明显,因此肥胖人群可选择耐力训练作为减肥的运动方式。  相似文献   

8.
由冷冻树莓制得粗酶液,测树莓中超氧化物歧化酶(SOD)的活力48.78U/g,其含量228.544μg/g鲜重。通过分别以新鲜的树莓、解冻1h树莓和加入浓度为10-6mol/L的抗坏血酸浸泡1min的树莓果实来探究酶活力。结果表明:相对条件下,冷冻树莓有最大的超氧化物歧化酶(SOD)活力。  相似文献   

9.
钱晓薇  钟晓芝 《科技通报》2006,22(6):771-774
以黄鳝为材料,研究了不同浓度的乙酸铜对黄鳝外周血白细胞数目、红细胞微核和核异常、以及肝脏过氧化氢酶(CAT)活性的影响。结果发现:在低剂量范围内,随着乙酸铜剂量的增加红细胞的微核率及核异常率逐渐增加、CAT活性升高,白细胞的数目则下降;至3.00 mg/kg剂量时红细胞的微核率、核异常率及CAT活性均达最大值,至1.00 mg/kg剂量时白细胞的数目达最低值;随着剂量的进一步增加红细胞的微核率、核异常率以及CAT活性反而下降,白细胞的数目则反而上升。结果说明一定剂量乙酸铜对黄鳝外周血细胞具有明显的遗传学毒性效应,且乙酸铜的作用具有双向性。  相似文献   

10.
陈朝银  刘丽  贲昆龙 《科技通报》2004,20(2):116-120
尿嘧啶DNA糖苷酶(UNG)是防止PCR假阳性反应的工具酶,参照限制性DNA内切酶活力测定法的原理(以完全消化DNA带型的最高稀释度为一个活性单位)和应用凝胶成像分析技术,建立了UNG酶活性的非同位素检测新方法.反应体积为20μL,底物dU ung(dU DNA)为165μg,加UNG后在适当温度下反应10min,使底物去除尿嘧啶,其AP位点经碱降解.通过琼脂糖凝胶电泳和凝胶成像分析,测定底物dU ung的残留量以测定UNG酶活性.酶活力以37℃每分钟降解1ngdU ung的酶量为一个单位,检测灵敏度为1ng,检测线性范围为0～165ng.该法简便易行、客观、精密,重复性好(CV<3%),结果显示直观.  相似文献   

11.
建立大鼠大强度耐力训练模型,研究羊肚菌对大强度耐力训练大鼠自由基代谢和运动能力的影响。测定了大鼠骨骼肌组织中SOD、GSH-Px、CAT三种抗氧化酶活性和脂质过氧化产物MDA含量,也测定了大强度耐力训练大鼠的力竭时间。结果表明：同运动对照组相比较,运动加药组大鼠骨骼肌抗氧化酶SOD、GSH-Px、CAT活性有显著性或极显著性上升,MDA含量有显著性下降,同时运动加药组大鼠力竭时间与运动对照组相比显著延长,表明在大强度耐力训练大鼠骨骼肌自由基代谢当中,补充羊肚菌发挥着良好的清除自由基和抗氧化效果。  相似文献   

12.
Red-cell glutathione (GSH) contents and enzyme activities of glutathione peroxidase (GSH-Px) as well as glutathione reductase (GSSG-R) were assayed in 30 normal subjects, 60 cirrhotic patients and 60 cancer patients. The mean GSH level and GSSG-R activity studied showed increased values in all diseased groups compared to normal values with significant elevations only in liver metastases group. Reduced enzyme activity of GSH-Px has been noted in both the cirrhotic groups but the decrease was significant only in alcoholic cirrhotic group. Decreased red-cell GSH-Px activities below the normal range were found in all the cancer patients with remarkable reduction in liver metastases than in hepatocellular carcinoma.  相似文献   

13.
Free radical mediated pathological processes may have a role in schizophrenia. Free radicals (oxy radicals, such as superoxide, hydroxyl ions and nitric oxide) cause cell injury, when they are generated in excess or when the antioxidant defense is impaired. Both these processes seem to be affected in schizophrenia. In this study we investigated erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities as antioxidant enzymes, malondialdehyde (MDA) as a sign of lipid peroxidation in schizophrenic patients. Activities of superoxide dismutase, catalase and malondialdehyde were greater in patients compared with the control group which may reflect increased oxidative stress in the brain tissue of schizophrenics. In the patient group erythrocyte SOD and CAT activities were weakly negative correlated with MDA concentration. These data reveal that antioxidant defense mechanisms might be impaired in schizophrenic patients. These findings also provide a theoretical basis for the development of novel therapeutic strategies, such as antioxidant supplementation.  相似文献   

14.
In this article,we report a few advances in the studies based on the solar vector magnetic field observations which were carried out mainly with the Solar Magnetic Field Telescope at the Huairou Solar Observing Station in the 1990s.(1) We developed necessary methodology and concepts in vector magnetogram analysis(Wang et al.1996).For the first time,we proposed to use the photospheric free magnetic energy to quantify the major flare productivity of solar active regions(ARs),and it had been proved to be the best parameter in representing the major flare activity.(2) We revealed that there was always a dominant sense of magnetic shear in a given AR(Wang 1994),which was taken as the premise of the helicity calculation in ARs;we made the first quantitative estimation of magnetic helicity evolution in ARs(Wang 1996).(3) We identified the first group of evidence of magnetic reconnection in the lower solar atmosphere with vector magnetic field observations and proposed a two-step reconnection flare model to interpret the observed association of flux cancellation and flares(Wang and Shi 1993).Efforts to quantify the major flare productivity of super active regions with vector magnetic field observations have been also taken.  相似文献   

15.
Magnetic hyperthermia therapy (MHT) is able to ablate tumors using an alternating magnetic field (AMF) to heat up magnetocaloric agents (e.g. magnetic nanoparticles) administered into the tumors. For clinical applications, there is still a demand to find new magnetocaloric agents with strong AMF-induced heating performance and excellent biocompatibility. As a kind of biocompatible and biodegradable material, magnesium (Mg) and its alloys have been extensively used in the clinic as an implant metal. Herein, we discovered that the eddy thermal effect of the magnesium alloy (MgA) could be employed for MHT to effectively ablate tumors. Under low-field-intensity AMFs, MgA rods could be rapidly heated, resulting in a temperature increase in nearby tissues. Such AMF-induced eddy thermal heating of MgA could not only be used to kill tumor cells in vitro, but also be employed for effective and accurate ablation of tumors in vivo. In addition to killing tumors in mice, we further demonstrated that VX2 tumors of much larger sizes growing in rabbits after implantation of MgA rods could also be eliminated after exposure to an AMF, illustrating the ability of MgA-based MHT to kill large-sized tumors. Moreover, the implanted MgA rods showed excellent biocompatibility and ∼20% of their mass was degraded within three months. Our work thus discovered for the first time that non-magnetic biodegradable MgA, an extensively used implant metal in clinic, could be used for effective magnetic thermal ablation of tumors under a low-field-intensity AMF. Such a strategy could be readily translated into clinical use.  相似文献   

16.
Curcumin has a potent antioxidant and anti-inflammatory properties that may suppress inflammatory component of atherosclerosis. It has been demonstrated that curcumin derivatives can reduce the formation of arterial fatty streaks in cholesterol-fed rabbits. Therefore in this study we evaluated the protective effects of Curcumin on the progression of atherosclerosis. 20 mature rabbits were included for this study; they were randomly divided into four groups each of 5. Group 1: (normal control) were fed corn pellets diet and tab water, group 2: (high cholesterol diet control) were kept on cholesterol rich diet (2% cholesterol) and tab water. Group 3: (cholesterol and rosuvastatin treated group) were kept on cholesterol rich diet (2% cholesterol) and 2.5 mg/kg/day Rosuvastatin dispersed in DW and given orally, group 4: (cholesterol and curcumin treated group) were kept on cholesterol rich diet (2% cholesterol) and 0.2% curcumin added with corn pellets. The study continued for 12 weeks then assessment of serum level of high sensitive C-reactive protein, ICAM1, VCAM1 and PCSK9 was carried out at the end of the study. Total antioxidant activity of curcumin was also determined. Histopathological examination of aortic tissues for atherosclerotic changes was also carried out. Atherogenic (cholesterol rich diet) induced an increment in serum level of TC, LDL, VLDL and TG with concomitant decrement in serum level of HDL and increased atherogenic index. Treatment with curcumin produced substantial reduction in serum TC, LDL, TG with no effect on HDL level thus decreased atherogenic index. Rabbits treated with curcumin showed a significant reduction in the serum level of high sensitive C-reactive protein, ICAM1, VCAM, PCSK9 serum expression and aortic total antioxidant capacity. Curcumin has a potent anti-inflammatory and anti- oxidant effects against atherosclerosis so exerts a protective role by decreasing lipid oxidation and inflammatory markers.  相似文献   

17.
The aim of this study is to evaluate the oxidative stress in predialysis, hemodialysis (HD) and peritoneal dialysis patients and to test the effects of antihypertensive drugs and volume control on oxidative stress parameters. The study was composed of five groups as follows: control group (n = 30), predialysis group (n = 30), peritoneal dialysis group (n = 30), hemodialysis group, (normotensive with strict volume control, n = 30), hemodialysis group (normotensive with medication, n = 30). Plasma malondialdehyde (MDA), erythrocyte superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx) and routine biochemical parameters were studied in all patients. Hemodialysis patients with strict volume control (HDvc) had lower levels of MDA than other patient groups (p < 0.001), and CAT, SOD values had highest level other patient groups (p < 0.001). The treatment of hypertension with strict volume control in chronic renal failure patients causes less damage to the antioxidant capacity.  相似文献   

18.
Thiamine or vitamin B1 is a well known coenzyme and nutrient necessary for the assembly and right functioning of several enzymes involved in the energy metabolism. The present study evaluates oxidative stress and prevalence of neurodegenerative conditions in the brain following TD. The study was carried out on mice (Musmusculus) in three groups, namely control and thiamine-deficient group for 8 (TD 8) and 10 (TD 10) days. Lipid peroxidation was determined in terms of reduced glutathione (GSH) and thiobarbituric acid reactive substance (TBARS). The level of antioxidant enzymes such as catalase (CAT), glutathione reductase, glutathione peroxidase (GPx), superoxide dismutase (SOD) and glutathione transferase (GST) were measured along with histopathological studies in all the groups. There was significant increase in the TBARS levels in group II (TD 8) and group III (TD 10) animals in comparison to controls (Group I). The GSH levels were found to be lower in both the treated groups. The level of antioxidant enzymes CAT (p < 0.001), glutathione reductase (p < 0.001), GPx (p < 0.001), SOD (p < 0.0001) were found to be significantly reduced in group III (TD 10) in comparison to controls. Histopathological studies showed moderated to extensive neuronal loss in group II and group III in comparison to control group. The increase in LPO and reduction in enzymes CAT, glutathione reductase, GPx, SOD, and GST following TD suggests mitochondrial dysfunction, neuronal loss acute oxidative stress that may impair the functioning of the brain along with the rise of neurodegenerative conditions in the affected animals.  相似文献   

19.
Manipulation of magnetic beads plays an increasingly important role in molecular diagnostics. Magnetophoresis is a promising technique for selective transportation of magnetic beads in lab-on-a-chip systems. We investigate periodic arrays of exchange-biased permalloy microstripes fabricated using a single lithography step. Magnetic beads can be continuously moved across such arrays by combining the spatially periodic magnetic field from microstripes with a rotating external magnetic field. By measuring and modeling the magnetophoresis properties of thirteen different stripe designs, we study the effect of the stripe geometry on the magnetophoretic transport properties of the magnetic microbeads between the stripes. We show that a symmetric geometry with equal width of and spacing between the microstripes facilitates faster transportation and that the optimal period of the periodic stripe array is approximately three times the height of the bead center over the microstripes.  相似文献   

20.
This study aimed to elucidate the mechanisms of melatonin to manage neurological damage in Alzheimer’s disease (AD) induced in ovariectomized rats. Forty adult female rats were enrolled in our study and were classified as; gonad intact control, ovariectomized control group, ovariectomized rats received melatonin, ovariectomized rats injected with AlCl3 to induce AD and AD-induced rats treated with melatonin. Hydrogen peroxide (H2O2), malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), B cell lymphoma 2 (Bcl-2), brain derived neurotrophic factor (BDNF), acetylcholinesterase (AchE) and acetylcholine (Ach) were estimated in the brain tissues of the different groups. Treatment of AD-induced rats with melatonin produced marked improvement in the most studied biomarkers which was confirmed by histological investigation of the brain. In Conclusion, melatonin significantly ameliorates the neurodegeneration characteristic of AD in experimental animal model due to its antioxidant, antiapoptotic, neurotrophic and anti-amyloidogenic activities.  相似文献   

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