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1.
Krishna Mohan Surapneni V. S. Chandrasada Gopan 《Indian journal of clinical biochemistry : IJCB》2008,23(1):41-44
The changes in the erythrocyte lipid peroxidation products (MDA), levels of glutathione (GSH), ascorbic acid and plasma vitamin
E (non enzymatic antioxidant parameters) and activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase
(GPX), catalase in erythrocytes and plasma glutathione - S - transferase (GST) activity were estimated in patients with rheumatoid
arthritis. This work was undertaken to assess oxidative stress and anti oxidant status in patients with rheumatoid arthritis.
It was observed that there was a significant increase in erythrocyte MDA levels, activities of SOD, GPX, plasma GST and a significant decrease in erythrocyte GSH, ascorbic acid, plasma vitamin E levels and catalse activity in
patients with rheumatoid arthritis when compared to controls. The results of our study suggests higher oxygen free radical
production, evidenced by increased MDA and decreased GSH, ascorbic acid, vitamin E and Catalase activity, support to the oxidative
stress in rheumatoid arthritis. The increased activities of antioxidant enzymes may be a compensatory regulation in response
to increased oxidative stress. 相似文献
2.
Sadanand B. Patil M. V. Kodliwadmath Sheela M. Kodliwadmath 《Indian journal of clinical biochemistry : IJCB》2008,23(1):45-48
The present study was designed to evaluate the lipid peroxidation and non-enzymatic antioxidants in pregnancyinduced hypertension
(PIH) compared with healthy normal pregnant and non-pregnant as controls. 75 cases were studied of which 25 were normal healthy
controls (Group A), 25 healthy pregnant women (Group B) of third trimester and 25 were PIH (Group C) of the same trimester.
In PIH, malondialdehyde a lipid peroxidation product was significantly increased as compared to normal pregnant and non-pregnant
controls. The nonenzymatic antioxidants like reduced glutathione, Vitamin-E, Vitamin-C and Vitamin-A were significantly decreased
in normal pregnants as compared to controls. A further significant decrease was noticed in PIH compared to normal pregnants.
A significant negative correlation was detected between lipid peroxidation and non-enzymatic antioxidant levels. Our study
clearly indicates a relationship between elevated lipid peroxidation, decreased non-enzymatic antioxidant in PIH. The measurement
of non-enzymatic antioxidants in plasma may be useful predictor of the likely development of PIH. 相似文献
3.
Tiwari AK Mahdi AA Zahra F Chandyan S Srivastava VK Negi MP 《Indian journal of clinical biochemistry : IJCB》2010,25(4):411-418
The present study was conducted to investigate the oxidant–antioxidant status in iron deficient pregnant anemic women. One
hundred thirty pregnant women with iron deficiency anemia (IDA) were divided into three groups, namely mild (50), moderate
(50) and severe (30) anemic along with pregnant healthy women as controls (50). The complete blood count, plasma lipid peroxidation
products, enzymatic and non-enzymatic antioxidants were measured according to respective protocols. The levels of complete
blood count, iron, ferritin along with antioxidant enzymes namely catalase, superoxide dismutase, glutathione peroxidase,
glutathione reductase and reduced glutathione were significantly reduced in all IDA groups. However, the level of oxidized
glutathione, lipid peroxides, protein carbonyls, conjugated dienes were found significantly increased in all anemic patients.
Antioxidant vitamins, namely C, E and A were also found significantly decreased in IDA patients. On the basis of our results,
it may be concluded that IDA tends to increase the pro-oxidant components, which may result in various complications including
peroxidation of vital body molecules resulting in increased risk for pregnant women as well as fetus. 相似文献
4.
K. Kaur G. Bedi M. Kaur Anil Vij Inderpreet Kaur 《Indian journal of clinical biochemistry : IJCB》2008,23(1):33-37
Coronary Artery Disease is the major cause of mortality and morbidity worldwide. Traditional risk factors account for only
half of the morbidity and mortality from coronary artery disease. There is substantial evidence that oxidative stress plays
the major role in the atherosclerotic process. The present study was undertaken to evaluate the level of lipid peroxidation
(by measuring malondialdehyde) and antioxidant enzymes (ceruloplasmin, glutathione, superoxide dismutase) in coronary artery
disease. Serum malondialdehyde levels and serum ceruloplasmin levels were significantly raised in all the subgroups of study
group as compared to control group (p<0.001). Whole blood glutathione levels and hemolysate superoxide dismutase activity
was significantly decreased in all the subgroups of study group as compared to control group (p<0.001). Above results suggests
that the patients of coronary artery disease show increased oxidative stress and decreased levels of antioxidant enzymes.
So it is recommended that the management protocol for coronary artery disease patients should include antioxidant supplementation
along with simultaneous lowering of lipid peroxidation. 相似文献
5.
K Sudha Ashalatha V Rao S. N. Rao Anjali Rao 《Indian journal of clinical biochemistry : IJCB》2002,17(1):34-41
Oxidative stress is implicated in the etiopathogenesis of a variety of human diseases. Therefore, in the present study, erythrocyte
lipid peroxidation, percentage hemolysis, antioxidant enzymes viz., glutathione reductase, glutathione peroxidase, superoxide
dismutase and plasma antioxidants viz., ceruloplasmin, vitamins A,E and C have been determined in 19 patients with tubercular
meningitis (TBM) and 50 normals. Six patients who were treated with antibiotics were considered for the follow up. The statistical
analysis was carried out by Mann Whitney U test and Wilcoxon rank sum test. Lipid peroxidation (P<0.02), percentage hemolysis
(P<0.001) and plasma ceruloplasmin (P<0.0001) of TBM patients were significantly higher, whereas erythrocyte glutathione reductase
(P<0.05) and plasma antioxidant vitamins A, E and C (P<0.01, P<0.05 respectively) were significantly lower than those of the
controls. In the follow up patients the glutathione reductase and catalase levels were significantly high (P<0.05) compared
to their pre-treated condition. Vitamin C and E levels have attained normal range. This study indicated that the blood antioxidant
status of TBM patients which was low compared to controls improved after treatment, suggesting the role of free radicals in
TBM. 相似文献
6.
Susceptibility of Schizophrenic patients to lipid peroxidation relative to healthy control subjects was investigated by measuring
the malondialdehyde (MDA) levels in plasma. The main finding was that Schizophrenic patients were more susceptible than control
subjects to oxidative damage as evident from increased MDA levels in plasma. Antioxidant levels are also depleted in Schizophrenic
patients when compared to normal subjects as evident from decreased levels of vitamins E and C in the plasma. Impaired antioxidant
defense and increased lipid peroxidation suggests that treatment with antioxidants (Vitamin E, Vitamin C, beta carotene) at
the initial stages of illness may prevent further oxidative injury and deterioration of associated neurological deficits in
Schizophrenia. 相似文献
7.
Damodara Reddy V Padmavathi P Gopi S Paramahamsa M Varadacharyulu NCh 《Indian journal of clinical biochemistry : IJCB》2010,25(4):419-424
The effect of Emblica officinalis fruit extract (EFE) against alcohol-induced hepatic damage in rats was investigated in the present study. In vitro studies showed that EFE possesses antioxidant as well nitric oxide (NO) scavenging activity. In vivo administration of alcohol (5 g/kg b.wt/day) for 60 days resulted increased liver lipid peroxidation, protein carbonyls, nitrite plus nitrate levels. Alcohol administration also significantly lowers the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase and reduced glutathione as compared with control rats. Administration of EFE (250 mg/kg body weight) to alcoholic rats significantly brought the plasma enzymes towards near normal level and also significantly reduced the levels of lipid peroxidation, protein carbonyls and restored the enzymic and non-enzymatic antioxidants level. This observation was supplemented by histopathological examination in liver. Our data indicate that the tannoid, flavonoid and NO scavenging compounds present in EFE may offer protection against free radical mediated oxidative stress in rat hepatocytes of animals with alcohol-induced liver injury. 相似文献
8.
S. D. Walwadkar A. N. Suryakar R. V. Katkam K. M. Kumbar R. D. Ankush 《Indian journal of clinical biochemistry : IJCB》2006,21(2):134-137
Generation of reactive oxygen species is an important factor in the development and maintenance of rheumatoid arthritis (RA)
in humans. This study was undertaken to investigate interplay among oxidants, antioxidants and pathogenesis of Rheumatoid
arthritis. Serum levels of lipid peroxides, nitric oxide, vitamin E and ratio of calcium/phosphorus in RA patients were determined
and compared with normal healthy controls. Significant increases in lipid peroxides (p<0.001) and nitric oxide (p<0.001) levels
were found in patients presenting with RA as compared to controls. Whereas significant decrease in vitamin E (P<0.001) and
calcium/phosphorus ratio (p<0.001) were found in Rheumatoid arthritis patients as compared to controls. Positive correlation
was found between lipid peroxides and nitric oxide as well as between vitamin E and calcium. While lipid peroxides and nitric
oxide were correlated negatively with vitamin E. whereas negative correlation was observed between MDA and Calcium/Phosphorus
ratio in patients with rheumatoid arthritis. Our findings suggest that there is a close association between bone loss and
oxidative threat in patients presenting with Rheumatoid arthritis. 相似文献
9.
Benedicta D’Souza Vivian D’Souza S. Sowmya G. Seema C. N. Hemalatha M. Anu G. Sajana 《Indian journal of clinical biochemistry : IJCB》2008,23(3):218-222
The study was conducted to find out the extent of lipid peroxidation and antioxidant status in ischemic stroke patients (ISPs)
with and without diabetes. Malondialdehyde (MDA) was studied as a marker of lipid peroxidation. Glutathione (GSH), uric acid
and ceruloplasmin were estimated to study the antioxidant potential of ISPs. Significantly higher levels of MDA were found
in both the groups of ISPs and the increase in MDA was more in ISPs without diabetes. GSH levels were decreased significantly
in both the groups of ISPs and maximum decline was found in ISPs with diabetes. Uric acid levels were significantly increased
in both the groups of ISPs. Ceruloplasmin levels were increased significantly in ISPs without diabetes, whereas its levels
were slightly decreased in ISPs with diabetes. A negative correlation was found between MDA and the antioxidants GSH, uric
acid and ceruloplasmin in ISPs with diabetes. This study suggests that there is an association between ischemic stroke and
increased oxidative stress and the antioxidant potential is impaired in both the groups of ISPs with and without diabetes. 相似文献
10.
G. M. Rao P Sumita M Roshni M. N. Ashtagimatt 《Indian journal of clinical biochemistry : IJCB》2005,20(1):198-200
It has been suggested that antioxidant systems are impaired in pregnancy induced hypertension and hence patients are exposed
to oxidative stress. In order to investigate the relationship between lipid peroxidation and certain antioxidant parameters
in blood of pregnancy induced hypertension (PIH) cases, 25 normotensive and 23 PIH samples were studied. In the present study,
thiobarbituric acid reactive substances showed a tendency to increase, however the increase remained statistically insignificant.
Plasma ascorbic acid level remained unaltered and Vitamin E showed a tendency to increase in the study group. The findings
implicate oxidative stress in the disease and cite the biochemical rationale for clinical trials of antioxidants to prevent
and treat pregnancy induced hypertension. 相似文献
11.
Neela Patil Vishwas Chavan N. D. Karnik 《Indian journal of clinical biochemistry : IJCB》2007,22(1):45-51
Free radicals play an important role in the pathogenesis of tissue damage in many clinical disorders, including atherosclerosis.
Antioxidants protect the body from damage caused by free radicals. In this study we investigated oxidative stress, antioxidants
and inflammatory molecules in patients with acute myocardial infarction. This study has been carried out on 106 patients with
acute myocardial infarction, (89 men and 17 females). The control group consisted of 50 healthy, age-matched subjects (40
men and 10 females). Levels of Glucose, lipid profile, glutathione reduced, glutathione peroxidase, Superoxide dismutase,
Glycosylated hemoglobin, fibrinogen, vitamin C, vitamin E, malondialdehyde, ceruloplasmin, adenosine deaminase, lysozyme and
sialic acid were measured. Malondialdehyde and ceruloplasmin levels were significantly high and antioxidants such as vitamin
C, vitamin E, glutathione reduced, glutathione peroxidase and superoxide dismutase were significantly decreased in diabetic
and non-diabetic AMI patients as compared with control (p<0.001). Inflammatory markers showed significant rise in diabetic
patients as compared with controls. Our results clearly show increased inflammation and oxidative stress in patients with
acute myocardial infarction. Depression of antioxidant system in these patients confirms this conclusion. 相似文献
12.
P. Faizal B. Satheeshan Milindkumar A.K. Adarsh R. Shilpa P. Roshni T. Remya K.T. Augusti 《Indian journal of clinical biochemistry : IJCB》2013,28(4):426-428
Extensive research has demonstrated the protective properties of antioxidants, which scavenge reactive oxygen species and their precursors, as well as up-regulate enzymes involved in the repair of cellular damage. Several case–control studies have showed higher blood levels of antioxidants and decreased oxidative stress in younger individuals when compared with older ones. Cell damage caused by free radicals appears to be a major contributor in aging and degenerative diseases of aging such as cancer, cardiovascular disease, cataracts, compromised immune system, rheumatoid arthritis and brain dysfunction. The objective of this study was to determine the variation of Circulating levels of selected antioxidants (enzymic and non enzymic) and oxidative stress marker in younger and older humans. The results showed that a majority of the younger age group participants showed a significant increase in enzymic and nonenzymic antioxidant status and a decrease in oxidative stress when compared with the older age group. 相似文献
13.
D. Vijayakumar K. Suresh S. Manoharan 《Indian journal of clinical biochemistry : IJCB》2005,20(1):52-55
The present study has investigated the levels of lipids, lipoprotein cholesterol (HDL and LDL cholesterol), thiobarbituric
acid reactive substances (TBARS) and vitamin E in plasma and erythrocyte membranes of twenty two clinically diagnosed adult
rheumatoid arthritis patients and an equal number of age matched healthy subjects. The levels of lipids and lipoprotein cholesterol
were markedly reduced in patients with rheumatoid arthritis as compared to healthy subjects. The altered lipid pattern may
be related to decreased lipoprotein cholesterol, fatty acids and impairment in antioxidant defence mechanism. 相似文献
14.
P. Rani K. Meena Unni J. Karthikeyan 《Indian journal of clinical biochemistry : IJCB》2004,19(2):103-110
The present paper focuses on assessing the levels of various enzymatic and non-enzymatic antioxidants in selected berries
of Indian sub-continent viz., gooseberry, grapes, orange and tomato. This study has revealed that orange, tomato and grapes
possess predominant quantities of enzymatic antioxidants namely SOD, catalase and glutathione peroxidase respectively. The
levels of antioxidants analyzed namely reduced glutathione, vitamin C and vitamin A were maximal in grapes, orange and tomato
respectively. All the extracts of berries included in the present study inhibited iron inducedin vitro lipid peroxidation in erythrocytes, the extend of inhibition being highest for gooseberry and orange compared to tomato and
grapes which could be attributed to their in-built antioxidant system. 相似文献
15.
M Maneesh H Jayalekshmi Sanjiba Dutta Amit Chakrabarti D M Vasudevan 《Indian journal of clinical biochemistry : IJCB》2005,20(2):62-67
The study was undertaken to evaluate the possible involvement of oxidative stress in the pathogenesis of ethanol induced testicular
atrophy in rats. Adult male rats were orally administered ethanol at a dose of 1.6 g/kg body weight/day for four weeks. Twenty-four
hours after the last treatment the rats were sacrificed using anesthetic ether. Testes were removed and weighed. Apoptosis
was studied by using the Feulgen reaction on 5 μ thin paraffin sections of testis. Testicular homogenate was prepared and
centrifuged. The supernatant was used for the estimation of extent of lipid peroxidation and antioxidant defense status. There
was significant reduction in body weight: and in testicular weight and diameter in ethanol treated rats. Extent of germ cell
apoptosis was significantly high in ethanol treated rats. Ethanol treated rats showed significantly high tissue TBARS level
and glutathione S-transferase activity; and low tissue ascorbic acid, reduced glutathione, superoxide dismutase, catalase,
glutathione peroxidase and glutathione reductase activities. Chronic ethanol administration resulted in high oxidative stress
in the testes either due to increased extent of lipid peroxidation or due to decreased antioxidant defenses, and thereby induces
germ cell apoptosis leading to testicular atrophy. 相似文献
16.
Rizwan Ahmad Anil K. Tripathi Payal Tripathi Ranjana Singh Sushma Singh Raj K. Singh 《Indian journal of clinical biochemistry : IJCB》2008,23(4):328-333
Chronic myeloid leukemia is a myeloproliferative disorder with a unique rearrangement, the Philadelphia chromosome. Oxidative
stress, a pervasive condition of an increased number of reactive oxygen species, is now recognized to be prominent feature
of various diseases and their progression. Thus antioxidants, which control the oxidative stress state, represent a major
line of defense regulating overall true state of health. The relationship between antioxidants status and levels of well-known
markers of oxidative stress that are measured as lipid peroxides and oxidized proteins reflect better health indices and postures.
The aim of this study was to evaluate the role of oxidative stress in pathophysiology of Chronic myeloid leukemia by measuring
the circulating plasma lipid peroxide levels in terms of malonyldialdehyde, total lipid hydroperoxide and oxidized proteins
as protein carbonyl whereas antioxidant status were estimated in terms of reduced glutathione and total thiol in plasma of
Chronic myeloid leukemia patients. The present study included 47 Chronic myeloid leukemia patients and 20 age-and sex-matched
healthy subjects. Out of 47 Chronic myeloid leukemia patients, 31 were in chronic phase (CML-CP) and 16 in accelerated phase
(CML-AP). The median age of Chronic myeloid leukemia patients was 33 years and that of controls was 32 years. Oxidative stress
and antioxidant status in plasma were evaluated by spectrophotometric procedures. There was a significant increase (p<0.05)
in plasma malonyldialdehyde, total lipid hydroperoxide and protein carbonyl levels in Chronic myeloid leukemia patients as
compared to healthy subjects. Our results also showed that plasma malonyldialdehyde and protein carbonyl levels were markedly
elevated (p<0.05) in both chronic phase (CML-CP) and accelerated phase (CML-AP) as compared to healthy volunteers. Antioxidant
status was found to be significantly decreased (p<0.05) in Chronic myeloid leukemia patients and its phases as compared to
healthy participants. It could be concluded that oxidative stress may be associated with the pathophysiology of Chronic myeloid
leukemia. 相似文献
17.
Arun Kumar Ramiah Sivakanesan Susil Gunasekera 《Indian journal of clinical biochemistry : IJCB》2008,23(3):296-298
The objective of the study was to observe the correlation between oxidative stress and antioxidants in myocardial infarct
patients with normal lipid profile. In the present study investigation of lipid peroxidation and antioxidants were done in
patients. This study was carried out on 165 AMI patients, (123 males and 42 females). The control group consisted on 165 normal
healthy age-sex matched subjects (123 males and 42 females). Levels of Superoxide dismutase, Glutathione Peroxidase, Catalase,
Malondialdehyde and Conjugated dienes were measured. The study found Superoxide dismutase, Glutathione Peroxidase, Catalase
were significantly lower in activities (p<0.001) in AMI patients compared to controls. The markers of lipid peroxidation namely
Malondialdehyde and Conjugated dienes were higher in AMI patients as compared to controls (p<0.001). The present study is
clearly suggestive of increased oxidative stress in AMI patients. Oxidative stress appears as an etiological factor for myocardial
infraction as a consequence the free radical scavengers levels are lowered in AMI patient. 相似文献
18.
A total of 41 healthy male pesticide sprayers exposed to different clases of pesticides for 3-5 years were compared with 21
controls matched for age and economic status with respect to free radical generation, lipid peroxidation, antioxidant status,
cholesterol, lipoprotein status and haematological profile. Plasma lipid peroxidation was estimated in the form of thiobarbituric
acid reactive substances (TBARS) produced. Significant increase in TBARS was observed in sprayers population when compared
with control subjects and the level of TBARS increased with increase in the duration of exposure. The levels of antioxidants
such as glutathione (GSH) were significantly depleted, whereas those of superoxide dismutase (SOD) were remarkably increased
than control population. Significant reduction in total cholesetrol, alteration in lipoprotein fractions and nonsignificant
changes in hematological parameters were observed. These results suggested that exposure to pesticidal residual drift augments
the free radical generation, and lipid peroxidation. Decline in non-enzymatic antioxidant and elevation of enzymatic antioxidant
were observed. Supplementation of α-tocopherol for 45 days resulted in the partial restoration of these biochemical changes
produced by pesticides. 相似文献
19.
D. P. Neupane S. Majhi L. Chandra S. Rijal Nirmal Baral 《Indian journal of clinical biochemistry : IJCB》2008,23(1):95-97
A glutathione redox cycle is a major antioxidant defense system for the detoxification of reactive oxygen species within erythrocytes.
Reactive oxygen species such as superoxide anions, hydrogen peroxide and hydroxyl radicals are generated as a host defense
mechanism for killing of engulfed Leishmania donovani, a causative agent of visceral leishmaniasis, are capable of damaging
lipids and other biomolecules when produced in excess. Erythrocytes are most vulnerable to Reactive oxygen species. In present
study we aimed to evaluate erythrocyte reduced glutathione (GSH) levels as an antioxidant and erythrocyte malondialdehyde
(MDA) as a marker of lipid peroxidation. The study included twenty-five Visceral leishmaniasis patients and they were followed
up after their complete chemotherapy with antileishmanial drugs (sodium stibogluconate) for 30 days. Forty six age and sex
matched healthy individuals were taken as controls. GSH levels in erythrocytes of visceral leishmaniasis patients were increased
in spite of significant increased erythrocyte MDA as compared to controls. Whereas erythrocyte GSH and MDA levels of follow
up patients were decreased as compared to patients before treatment groups. We concluded that visceral leishmaniasis patients
are in oxidative stress which most likely induces the endogenous antioxidant such as GSH or its poor utilization by cells. 相似文献
20.
M Maneesh H Jayalekshmi T Suma S Chatterjee A Chakrabarti T A Singh 《Indian journal of clinical biochemistry : IJCB》2005,20(1):129-130
Evidence of increased oxidative stress in patients of osteoarthritis in comparison with healthy control subjects was investigated
by measuring the thiobarbituric acid reactive substances (TBARS), vitamin C, reduced glutathione (GSH) and the activities
of superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) in erythrocytes. It was observed that osteoarthritis
patients were more susceptible to oxidative damage than controls as evident from increased TBARS and decreased ascorbic acid,
GSH, catalase and GPx in erythrocytes. Significant increase in SOD activity found in patients might be an adaptive response.
With the understanding of the role of antioxidants in arthritis, it is becoming increasingly clear that these agents seem
to be beneficial in osteoarthritis. 相似文献