共查询到20条相似文献，搜索用时 31 毫秒
1.
Objective: In addition to pH regulation, Na+/H+ exchange (NHE) has been shown to facilitate cell growth and proliferation. However, the effects of long-term inhibition of
Na+/H+ exchange on cardiac structural and functional remodeling post myocardial infarction (MI) are still controversial. The present
study was therefore carried out to further investigate the effects of long-term treatment with cariporide, a specific inhibitor
of NHE-1, on cardiac remodeling after MI in rats; Methods: Male Wistar rats that underwent coronary ligation were randomly
selected for cariporide treatment starting 6 h after induction of MI or no treatment. Treatment was continued up to 6 weeks
post MI, after which, the arterial, venous and left ventricular catheters were chronically implanted. Twenty-four h later,
after hemodynamic signals were recorded in conscious rats, they were sacrificed and hearts were taken out for morphological
examinations; Results: Cariporide treatment decreased the heart weight and heart weight to body weight ratio (bothP<0.05), decreased left ventricular end-diastolic pressure (P<0.001), improved myocardial contractility (dP/dt
max) (P<0.05) and tended to increase the survival of treated rats compared to that of untreated infarct rats; Conclusion: The results
of the present study indicate that the long-term inhibition of NHE with cariporide can attenuate cardiac structural remodeling
and improve left ventricular dysfunction in infarcted rats, and suggest that Na+/H+ exchange inhibition could be an effective therapeutic strategy for myocardial infarction-induced heart failure. 相似文献
2.
Objective: Myocardial infarction (MI) is the main cause of heart failure, but the relationship between the extent of MI and cardiac function has not been clearly determined. The present study was undertaken to investigate early changes in the electrocardiogram associated with infarct size and cardiac function after MI. Methods: MI was induced by ligating the left anterior descending coronary artery in rats. Electrocardiograms, echocardiographs and hemodynamic parameters were assessed and myocardial infarct size was measured from mid-transverse sections stained with Masson's trichrome. Results: The sum of pathological Q wave amplitudes was strongly correlated with myocardial infarct size (r = 0.920, P < 0.0001), left ventricular ejection fraction (r = (0.868, P < 0.0001) and left ventricular end diastolic pressure (r = 0.835, P < 0.0004). Furthermore, there was close relationship between MI size and cardiac function as assessed by left ventricular ejection fraction (r = (0.913, P < 0.0001) and left ventricular end diastolic pressure (r = 0.893, P < 0.0001). Conclusion: The sum of pathological Q wave amplitudes after MI can be used to estimate the extent of MI as well as cardiac function. 相似文献
3.
Ze-wei Tao Long-gui Li Zhao-hua Geng Tao Dang Shan-jun Zhu 《Journal of Zhejiang University. Science. B》2010,11(4):238-248
Therapeutically delivered mesenchymal stem cells (MSCs) improve ventricular remodeling. However,the mechanism underlying MSC cardiac remodeling has not been clearly determined. Congestive heart failure (CHF) was induced in rats by cauterization of the left ventricular free wall. MSCs were cultured from autologous bone marrow and injected into the border zone and the remote myocardium 5 d after injury. Ten weeks later,when compared with sham operation,CHF significantly increased nucleus mitotic index,capilla... 相似文献
4.
Kun ZHAO Jing ZHANG Tianhua XU Chuanxi YANG Liqing WENG Tingting WU Xiaoguang WU Jiaming MIAO Xiasheng GUO Juan TU Dong ZHANG Bin ZHOU Wei SUN Xiangqing KONG 《Journal of Zhejiang University. Science. B》2021,22(10):818
ObjectiveCardiac hypertrophy and fibrosis are major pathological manifestations observed in left ventricular remodeling induced by angiotensin II (AngII). Low-intensity pulsed ultrasound (LIPUS) has been reported to ameliorate cardiac dysfunction and myocardial fibrosis in myocardial infarction (MI) through mechano-transduction and its downstream pathways. In this study, we aimed to investigate whether LIPUS could exert a protective effect by ameliorating AngII-induced cardiac hypertrophy and fibrosis and if so, to further elucidate the underlying molecular mechanisms.MethodsWe used AngII to mimic animal and cell culture models of cardiac hypertrophy and fibrosis. LIPUS irradiation was applied in vivo for 20 min every 2 d from one week before mini-pump implantation to four weeks after mini-pump implantation, and in vitro for 20 min on each of two occasions 6 h apart. Cardiac hypertrophy and fibrosis levels were then evaluated by echocardiographic, histopathological, and molecular biological methods.ResultsOur results showed that LIPUS could ameliorate left ventricular remodeling in vivo and cardiac fibrosis in vitro by reducing AngII-induced release of inflammatory cytokines, but the protective effects on cardiac hypertrophy were limited in vitro. Given that LIPUS increased the expression of caveolin-1 in response to mechanical stimulation, we inhibited caveolin-1 activity with pyrazolopyrimidine 2 (pp2) in vivo and in vitro. LIPUS-induced downregulation of inflammation was reversed and the anti-fibrotic effects of LIPUS were absent.ConclusionsThese results indicated that LIPUS could ameliorate AngII-induced cardiac fibrosis by alleviating inflammation via a caveolin-1-dependent pathway, providing new insights for the development of novel therapeutic apparatus in clinical practice. 相似文献
5.
Effect of matrine and carvedilol on collagen and MMPs activity of hypertrophy myocardium induced by pressure overload 总被引：3，自引：0，他引：3
Zhang YJ Xiang MX San J Cheng G Wang SS 《Journal of Zhejiang University. Science. B》2006,7(3):245-250
INTRODUCTION Matrine, a monomer of traditional Chinese medi-cine, comes from leguminosae plants such as Kusheng, is quinoilizidine with four-loop and molecular formula of C15H24N20. Matrine has been proved to have anti-arrhythmia (Xu et al., 2004), anti-hypoxia and decreasing heart rate effects (Zhang et al., 1990a; 1990b) in many animal experiments, and has the role of inducing calmness (Luo et al., 2001) and lowering body temperature (Tao and Wan, 1992). Traditional Chinese medicin… 相似文献
6.
Optimal time for mesenchymal stem cell transplantation in rats with myocardial infarction 总被引：1，自引：0，他引：1
Jiang CY Gui C He AN Hu XY Chen J Jiang Y Wang JA 《Journal of Zhejiang University. Science. B》2008,9(8):630-637
Background:Bone marrow mesenehymal stem cell(MSC)transplantation is a promising strategy in the treatment of myocardial infarction(MI).However,the time for transplanting cells remains controversial.The aim of this study was to find an optimal time point for cell transplantation.Methods:MSCs were isolated and cultured from Sprague-Dawley(SD) rats.MI model was set up in SD rats by permanent ligation of left anterior descending coronary artery.MSCs were directly injected into the infarct berder zone at 1 h,1 week and 2 weeks after MI,respectively.Sham-operated and MI centrel groups received equal volume of phosphate buffered saline(PBS).At 4 weeks after MI,cardiac function Was assessed by echocardiography;vessel density Was analyzed on hematoxylin-eosin stained slides by light microscopy;the apoptosis of cardiomyocytes Was evaluated by terminal deoxynucleotidy1 transferase-mediated dUTP nick end-labeling(TUNEL) assay;the expressions of proteins were analyzed by Western blot.Results:MSC transplantation improved cardiac function.reduced the apoptosis of cardiomyocytes and increased vessel density.These benefits were more obvious in l-week group than in 1-h and 2-week groups.There are more obvious increases in the ratio of bc1-2/bax and the expression of vascular endothelial growth factor(VEGF)and more obvious decreases in the expression of cleaved-caspase-3 in 1-week group than those in other two groups.Conclusion:MSC transplantation was beneficial for the recovery of cardiac function.MSC transplantation at l week post-MI exerted the best effects on increases of cardiac function,anti-apoptosis and angiogenesis. 相似文献
7.
ＩＮＴＲＯＤＵＣＴＩＯＮＤｏｘｏｒｕｂｉｃｉｎ(ＤＯＸ)ｉｓａｋｉｎｄｏｆａｎｔｈｒａｃｙ ｃｌｉｎｅｓａｎｔｉｂｉｏｔｉｃｓｗｈｏｓｅａｎｔｉｎｅｏｐｌａｓｔｉｃｓｐｅｃｔｒｕｍｉｓｂｒｏａｄａｎｄｅｆｆｅｃｔｉｓｓｔｒｏｎｇ.Ｉｔｈａｓｇａｉｎｅｄｗｉｄｅ ｓｐｒｅａｄｕｓｅｉｎｔｈｅｔｒｅａｔｍｅｎｔｏｆｃｈｉｌｄｈｏｏｄｌｅｕｋｅ ｍｉａａｎｄｓｏｌｉｄｔｕｍｏｒｓ.Ｈｏｗｅｖｅｒ,ｉｔｓｃｌｉｎｉｃａｌｕｓｅｉｓｌｉｍｉｔｅｄｂｙｉｔｓｃａｒｄｉｏｔｏｘｉｃｉｔｙ .Ｔｈｅｃａｌｃｉｕｍｏｖｅｒ… 相似文献
8.
ＩＮＴＲＯＤＵＣＴＩＯＮＢｅｓｉｄｅｓｉｎｔｒｉｎｓｉｃｇａｓｔｒｏｉｎｔｅｓｔｉｎａｌａｂｎｏｒｍａｌｉ ｔｉｅｓ,ｄｙｓｍｏｔｉｌｉｔｙｏｆｇａｓｔｒｏｉｎｔｅｓｔｉｎａｌｔｒａｃｔｓｃａｎａｌｓｏｂｅｃａｕｓｅｄｂｙｅｎｄｏｃｒｉｎｏｌｏｇｉｃｐｒｏｂｌｅｍｓｓｕｃｈａｓｄｉ ａｂｅｔｅｓｍｅｌｌｉｔｕｓ,ｇａｓｔｒｏｐａｒｅｓｉｓｆｏｒｅｘａｍｐｌｅ (Ｒａｔｈ ｍａｎｎｅｔａｌ.,1 991 ) .Ｔｈｅｒｅｌａｔｉｏｎｓｈｉｐｂｅｔｗｅｅｎｇａｓｔｒｏｉｎｔｅｓｔｉｎａｌｄｙｓｆｕｎｃｔｉｏｎａｎｄｐ… 相似文献
9.
Human bone marrow-derived mesenchymal stem cells transplanted into damaged rabbit heart to improve heart function 总被引：18，自引：0，他引：18
INTRODUCTION Congestive heart failure is the end stage of manycardiovascular diseases. Myocardial infarction (MI)is a life-threatening event that may cause suddencardiac death and heart failure. Despite considerableadvances in diagnosis and treatment of heart disease,cardiac dysfunction after MI is still the majorworldwide cardiovascular disorder. Damaged myo-cardium after acute MI is gradually replaced by fi-brotic noncontractile cells to form scar tissue. Thedeveloping ventricul… 相似文献
10.
Xiao-jing Zhao Zhuo Pen Ping Li Er-xiu Chen Jian Liu Yan-xia Gao Yun-xia Ren Li-jun Li 《Journal of Zhejiang University. Science. B》2013,14(6):505-510
Objective
We investigated the influence of β-receptor blocker metoprolol on return of spontaneous circulation (ROSC) during cardiopulmonary resuscitation (CPR) in rats with induced myocardial infarction (MI).Methods
Male Sprague-Dawley rats were randomly divided into three groups: the sham-operated group, the MI group without metoprolol, which was fed the vehicle, and the MI+metoprolol group receiving intragastric metoprolol. Each group was further divided randomly into three subgroups, depending on the dosage of epinephrine administered during subsequent CPR applied after the induction of asphyxial cardiac arrest.Results
The ROSC rate was significantly decreased in the low dose subgroup of MI group, unchanged in the medium dose subgroup of MI group, and significantly decreased in the high dose subgroup of MI group, compared with the same dose subgroup of sham-operated group. MI+metoprolol group had a lower ROSC rate than MI group in the medium dose subgroup, and a higher ROSC rate than MI group in the high dose subgroup. There was no difference in blood K+ values of successful rats between MI group and MI+metoprolol group. The rats with successful CPR had lower blood K+ values than rats with unsuccessful CPR in each of the three treatment groups.Conclusions
Metoprolol administered to MI rats over a long period significantly improved ROSC rates under an appropriate dose of epinephrine during CPR. An increasing high blood K+ value would attenuate the rate of a successful CPR. 相似文献11.
目的 :探讨高血压心肌肥厚过程中Na+ -K+ -ATPase活性的变化。方法 :用酶活性测定的方法 ,测定 12周、16周自发性高血压大鼠 (SHR)大鼠和Wistar大鼠的心肌Na+ -K+-ATPase酶的活性。结果 :(1)不同年龄SHR大鼠血压和左心室肥厚指数明显高于同龄Wis tar大鼠 ,但SHR大鼠间无显著差异。 (2 )SHR大鼠心肌Na+ -K+ -ATPase活性随年龄增加而明显降低 ,但两种大鼠 12周龄、16周龄间无显著差异。结论 :SHR大鼠血压和左心室肥厚指数明显增加 ,Na+ -K+ -ATPase活性发生下降 ,但是 12周与 16周间无明显差异。 相似文献
12.
Congestive heart failure (CHF) has emerged as a major worldwide epidemic and its main causes seem to be the aging of the population and the survival of patients with post-myocardial infarction. Cardiomyocyte dropout (necrosis and apoptosis) plays a critical role in the progress of CHF; thus treatment of CHF by exogenous cell implantation will be a promising medical approach. In the acute phase of cardiac damage cardiac stem cells (CSCs) within the heart divide symmetrically and/or asym-metrically in response to the change of heart homeostasis, and at the same time homing of bone marrow stem cells (BMCs) to injured area is thought to occur, which not only reconstitutes CSC population to normal levels but also repairs the heart by dif-ferentiation into cardiac tissue. So far, basic studies by using potential sources such as BMCs and CSCs to treat animal CHF have shown improved ventricular remodelling and heart function. Recently, however, a few of randomized, double-blind, pla-cebo-controlled clinical trials demonstrated mixed results in heart failure with BMC therapy during acute myocardial infarction. 相似文献
13.
目的 :观察中药复方心衰康煎液抗心衰的作用。方法 :取 4 0只成年Wistar大鼠 ,利用腹腔注射盐酸阿霉素的方法 ,连续 6周 ,复制心衰大鼠模型 ,再用心衰康煎液连续治疗 2 8d。利用北京微信达设计制造的Pclab实验记录分析系统观察和分析各组大鼠左心室内压、心电图 ,并观察心肌细胞的病理变化。结果 :心衰康煎液大、小剂量组和模型对照组比较左心室收缩内压显著性升高 (P <0 .0 1) ,病理学观察可见大、小剂量组心肌细胞的变性坏死均有明显减轻。结论 :心衰康煎液可减轻阿霉素对心肌的毒性作用 ,可明显改善心衰大鼠的心功能 ,表明该方有良好的抗心衰作用。 相似文献
14.
Cell therapy in congestive heart failure 总被引：5，自引：0，他引：5
Congestive heart failure （CHF） has emerged as a major worldwide epidemic and its main causes seem to be the aging of the population and the survival of patients with post-myocardial infarction. Cardiomyocyte dropout （necrosis and apoptosis） plays a critical role in the progress of CHF; thus treatment of CHF by exogenous cell implantation will be a promising medical approach. In the acute phase of cardiac damage cardiac stem cells （CSCs） within the heart divide symmetrically and/or asymmetrically in response to the change of heart homeostasis, and at the same time homing of bone marrow stem cells （BMCs） to injured area is thought to occur, which not only reconstitutes CSC population to normal levels but also repairs the heart by differentiation into cardiac tissue. So far, basic studies by using potential sources such as BMCs and CSCs to treat animat CHF have shown improved ventricular remodelling and heart function. Recently, however, a few of randomized, double-blind, placebo-controlled clinical trials demonstrated mixed results in heart failure with BMC therapy during acute myocardial infarction. 相似文献
15.
Nie SP Wang X Qiao SB Zeng QT Jiang JQ Liu XQ Zhu XM Cao GX Ma CS 《Journal of Zhejiang University. Science. B》2010,11(12):895-904
Objective: Angiogenic therapy is emerging as a potential strategy for the treatment of ischemic heart disease but is limited by a relatively short half-life of growth factors.Fibrin glue (FG) provides a reservoir for controlledrelease of growth factors.The aim of this study was to evaluate the effects of basic fibroblast growth factor (bFGF) incorporating FG on angiogenesis and cardiac performance in a canine infarct model.Methods: Acute myocardial infarction was induced by ligation of the left anterior descending coronary artery (LAD).Group I (n=6) underwent ligation of LAD alone.In Group II,transmural channels were created in the infarct area (n=6).In Group III,nontransmural channels were created to locate FG cylinders containing bFGF (n=6).Eight weeks after operation,myocardial perfusion was assessed by single photon emission computed tomography,cardiac function by echocardiography,and vascular development by immunohistochemical staining.Results: Total vascular density and the number of large vessels (internal diameter ≥50 μm) were dramatically higher in Group III than in Groups I and II at eight weeks.Only the controlled-release group exhibited an improvement in regional myocardial perfusion associated with lower defect score.Animals in Group III presented improved cardiac regional systolic and diastolic functions as well as global systolic function in comparison with the other two groups.Conclusions: Enhanced and sustained angiogenic response can be achieved by controlled-release bFGF incorporating FG within transmyocardial laser channels,thus enabling improvement in myocardial perfusion and cardiac function. 相似文献
16.
Jie Cui Zhi Li Ling-bo Qian Qin Gao Jue Wang Meng Xue Xiao-e Lou Iain C. Bruce Qiang Xia Hui-ping Wang 《Journal of Zhejiang University. Science. B》2013,14(6):487-495
Objective
To investigate the beneficial effect of bicyclol on rat hearts subjected to ischemia-reperfusion (IR) injuries and its possible mechanism.Methods
Male Sprague-Dawley rats were intragastrically administered with bicyclol (25, 50 or 100 mg/(kg·d)) for 3 d. Myocardial IR was produced by occlusion of the coronary artery for 1 h and reperfusion for 3 h. Left ventricular hemodynamics was continuously monitored. At the end of reperfusion, myocardial infarct was measured by 2,3,5-triphenyltetrazolium chloride (TTC) staining, and serum lactate dehydrogenase (LDH) level and myocardial superoxide dismutase (SOD) activity were determined by spectrophotometry. Isolated ventricular myocytes from adult rats were exposed to 60 min anoxia and 30 min reoxygenation to simulate IR injuries. After reperfusion, cell viability was determined with trypan blue; reactive oxygen species (ROS) and mitochondrial membrane potential of the cardiomyocytes were measured with the fluorescent probe. The mitochondrial permeability transition pore (mPTP) opening induced by Ca2+ (200 μmol/L) was measured with the absorbance at 520 nm in the isolated myocardial mitochondria.Results
Low dose of bicyclol (25 mg/(kg·d)) had no significant improving effect on all cardiac parameters, whereas pretreatment with high bicyclol markedly reduced the myocardial infarct and improved the left ventricular contractility in the myocardium exposed to IR (P<0.05). Medium dose of bicyclol (50 mg/(kg·d)) markedly improved the myocardial contractility, left ventricular myocyte viability, and SOD activity, as well decreased infarct size, serum LDH level, ROS production, and mitochondrial membrane potential in rat myocardium exposed to IR. The reduction of ventricular myocyte viability in IR group was inhibited by pretreatment with 50 and 100 mg/(kg·d) bicyclol (P<0.05 vs. IR), but not by 25 mg/(kg·d) bicyclol. The opening of mPTP evoked by Ca2+ was significantly inhibited by medium bicyclol.Conclusions
Bicyclol exerts cardioprotection against IR injury, at least, via reducing oxidative stress and its subsequent mPTP opening. 相似文献17.
淋巴液对正常血压大鼠的平均动脉压[MAP]、左室收缩压[LVSP]及左室舒张末压[LVEDP]与生理盐水对照组各项指标相比无显著差异[P>>0.05];而对失血性休克大鼠的MAP、LVSP具有显著回升作用[P<0.01],而对休克时LVEP的恢复无明显作用[P>0.05]。提示:淋巴液只能提高休克心脏的收缩功能,在低血容量情况下对休克心脏的舒张功能无明显影响,对正常心脏的收缩和舒张功能均无明显影响。 相似文献
18.
应用实时三维超声心动图评价冠心病患者经皮冠状动脉介入术(PCI)后左室收缩功能的变化。研究对象分为两组,正常组14例为冠脉造影无异常发现者,冠心病组49例行冠脉内支架治疗。所有患者均于术前及术后14天行实时三维超声检查,测得左室收缩末容积(LVESV)、左室舒张末容积(LVEDV)及左室射血分数(LVEF)。结果,正常组术前与术后LVESV、LVEDV及LVEF差异无统计学意义;冠心病组术后LVESV、LVEDV显著小于术前(P<0.001),LVEF显著高于术前(P<0.001)。结果表明,实时三维超声心动图能准确评价左室收缩功能,冠心病患者PCI后左室收缩功能明显改善。 相似文献
19.
To investigate the effect of doxorubicin(DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR)Ca2+ transport proteins and the mechanism of taurine(Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX , 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function , concentration of calcium in cardiomyocytes (Myo[Ca2+]i), activity of SR Ca2+-ATPase(SERCA2a), level of SERCA2a mRNA and Ca2+ released channels(RYR2)mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR Ca2+-ATPase and level of SERCA2a mRNA decreased , while Myo[Ca2+]i increased in DOX-treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca2+]i and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX-induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA. 相似文献
20.
大鼠重度失血性休克30min后,左心室内压的最大上升速率( dp/dt max)和左心室收缩压(LVSP)都显著下降(P<0.01),而左心室舒张末压(LVEDP)却有所增高(P<0.05)。说明心肌的收缩功能和舒张功能均出现障碍,给少量淋巴液后(用量为0.5ml/100g体重)使休克时下降的左心室内压的最大上升速率和左心室收缩压显著回升(P<0.05),而增高的左心室舒张末压回落不明显,提示淋巴液可改善休克心肌的收缩功能障碍,但对低血容量情况下的心肌舒张功能无明显改善。 相似文献