共查询到20条相似文献，搜索用时 15 毫秒
1.
Fruit ripening is a complex process and is regulated by many factors.Ethylene and polygalacturonase (PG),lipoxygenase (LOX),expansin (EXP) are all critical regulating factors in fruit ripening and softening process.With antisense,ACS tomato,Nr mutant tomato and cultivated tomato as materials,Northern blot hybridization showed that PG,LeEXP1 and LOX expressed differently in different parts of cultivated tomato fruit during ripening,which was related to fruit ripening.The ripening process of columella and radial pericarp was faster than pericarp.In both Nr mutant and antisense ACS transgenic tomato fruit,expression levels of PG,LeEXP1 and LOX were generally lower than those in cultivated fruit but still related to fruit ripening.The expression levels of PG,LeEXP1 and LOX increased in the mature green tomato fruits after 0.5 h treatment with ethylene ( 100 μL/L).These results indicate that gene expression of PG,LeEXP1 and LOXwere positively regulated by ethylene.The time and cumulative effect of the concentration exists in the expression of PG regulated by ethylene.The regulation of LOX expression mainly depended on the fruit development after great amount of ethylene was produced.PG played a major role in ripening and softening of tomato fruit,and cooperated with the regulation of EXP and LOX. 相似文献
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Characteristics of fruit ripening in tomato mutant epi 总被引：1，自引：0，他引：1
INTRODUCTION As a gaseous phytohormone, ethylene plays an important role in plant growth and development. Eth-ylene can alter plant physiology and morphology due to its effect of regulating gene expression (Moc-tezuma et al., 2003). Such regulation apparently de-pends on the effect of the normal ability of the plant tissues on ethylene perception and signal transduction.Impairment of the perception and signal transduction pathway(s) would thus ultimately lead to alterations in the plant… 相似文献
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Wei-hong Sun Yong Wang Hua-gang He Xue Li Wan Song Bin Du Qing-wei Meng 《Journal of Zhejiang University. Science. B》2013,14(7):578-585
Ascorbate peroxidases are directly involved in reactive oxygen species (ROS) scavenging by reducing hydrogen peroxide to water. The tomato thylakoid-bound ascorbate peroxidase gene (StAPX) was introduced into tobacco. RNA gel blot analysis confirmed that StAPX in tomato leaves was induced by methylviologen-mediated oxidative stress. The sense transgenic seedlings exhibited higher tAPX activity than that of the wild type (WT) plants under oxidative stress conditions, while the antisense seedlings exhibited lower tAPX activity. Lower APX activities of antisense transgenic seedlings caused higher malondialdehyde contents and relative electrical conductivity. The sense transgenic seedlings with higher tAPX activity maintained higher chlorophyll content and showed the importance of tAPX in maintaining the optimal chloroplast development under methylviologen stress conditions, whereas the antisense lines maintained lower chlorophyll content than WT seedlings. Results indicated that the over-expression of StAPX enhanced tolerance to methylviologen-mediated oxidative stress in sense transgenic tobacco early seedlings, whereas the suppression of StAPX in antisense transgenic seedlings showed high sensitivity to oxidative stress. 相似文献
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首次利用激光拉曼技术对微量乙烯气体进行测试和分析,得到500 cm-1~3500 cm-1内乙烯气体的拉曼光谱。结果表明,乙烯气体的拉曼光谱有明显特征峰,这一性质为进一步研究果蔬散发乙烯气体的检测提供了依据。 相似文献
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本文比较分析了滇池两种水葫芦的形态特征和生物量分配。结果表明,紫根水葫芦和普通水葫芦在根长、叶柄长、叶片长和叶片宽以及根冠比等的差异均达到极显著(p0.01)。紫根水葫芦的各个部分的干重都比普通水葫芦低,其中全株、叶柄、叶片干重的差异达到极显著水平(p0.01),根的干重差异达到了显著水平(p0.05)。紫根水葫芦和普通水葫芦在生物量分配上存在显著差异。两种水葫芦叶柄/全株和叶/全株差异达到显著水平(p0.05),根/全株、叶柄/根、叶/根、叶柄/叶表现出极显著差异(p0.01)。研究结果表明诱变育种措施显著地改变了水葫芦的生物量分配,较大的根系生物量分配应该是紫根水葫芦净化富营养化水体的生物学基础。 相似文献
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目的：观察开心散含药血清对皮质酮（corticosterone）损伤大鼠大脑皮质星形胶质细胞CTXTNA2的影响。方法：制备开心散含药血清（500mg·kg-1）、阳性药氟西汀含药血清（10mg·kg-1）、普通大鼠血清。细胞实验中采用皮质酮损伤CTXTNA2细胞建立体外抑郁模型,MTT法检测开心散对皮质酮所致细胞损伤的影响以及磷脂酰肌醇3-激酶（phosphatidylinositol 3-kinase,P13K）信号通路抑制剂的干预作用;采用Western Blotting法检验相关信号通路蛋白表达变化情况。结果：皮质酮浓度为200μm01·L-1时,细胞活力抑制率可稳定在60％,浓度依赖性较好;开心散含药血清均能够显著提高皮质酮损伤细胞的存活率,而给予P13K相关通路的抑制剂后,开心散保护作用被逆转,Western Blotting结果显示给予开心散含药血清能够逆转皮质酮损伤后P13K及蛋白激酶B（protein kinase B,AKT）1／2蛋白表达下降的趋势。结论：开心散含药血清对皮质酮所致的CTXTNA2细胞损伤具有明显保护作用,其保护机制可能与P13K信号通路有关。 相似文献
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通过在厦门地区对3个马来西亚番木瓜品种进行引种试验,结果表明,引种的马来10号和马来2号为中果型,植株均具有很好的结果性能,产量高,品质好,耐贮运,植株耐环斑花叶病.马来10号茎干、叶柄呈紫红色,节间距较短,较具矮生.2个品种均具有很好的推广应用前景.引种的蜜红3号为小果型,植株亦具有良好的结果性能,品质优,但产量较低,且存在果形较不稳定、畸形花果较多等问题,该品种不适宜推广栽培. 相似文献
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乙烯式卤代烃难发生亲核取代反应,反应历程研究受到限制.在双键碳中引入超离去基,反应顺利进行,且立体构型发生反转,证明该反应为SN2历程. 相似文献
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将萤火虫萤光素酶基因重组到质粒pBR325中,通过“三亲交配”和同源重组,将萤光素酶基因整合到Ti质粒pGV3850:1103。通过改良的叶盘转化法,用萤光素酶基因转化单子叶植物花叶芋,获得转基因植株。对再生植株的胭脂碱测定、新霉素磷酸转移酶分析、DNA点杂交、萤光素酶基因活性测定,证明萤光素酶基因已转化到花叶芋中并进行了表达。 相似文献
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Li FZ Jin SH Hu GC Fu YP Si HM Jiang DA Sun ZX 《Journal of Zhejiang University. Science. B》2005,6(8):803-811
A rice pse(t) (premature senescence, tentatively) mutant line, was isolated from 4500 independent T-DNA inserted transgenic lines. The symptoms of premature senescence appeared more severely than those of the control plants (Zhonghua 11,japonica) at the last development stage. To characterize the mutant and provide basic information on the candidate genes by mapping to a physical region of 220-kb, experiments were carried out in two phytotrons under controlled temperature of 24 ℃ and28 ℃, respectively. The content of chlorophyll, soluble protein and MDA (malondialdehyde), net photosynthesis, the antioxidant enzyme activities of SOD (superoxide dismuase) (EC 1.15.1.1) and POD (peroxidase) (EC 1.11.1.7) and the peptidase activities of leaves were measured from top to bottom according to the leaf positions at the flowering stage. Compared with the control plant,the mutant showed the following characteristics: (1) Higher net photosynthesis rate (Pn) appeared in the 1 st and 2nd leaves, contents of chlorophyll and soluble protein were also higher in the 1 st leaf; (2) The activities of SOD, POD and peptidase were higher according to the leaf position from top to bottom; (3) The symptom of premature senescence was accelerated in the mutant at 28℃treatment. The MDA content and the SOD and POD activities between the 24 ℃ and 28 ℃ treatment mutants were not significantly different. Content of chlorophyll and soluble protein of leaves mutant decreased rapidly at 28 ℃ treatment. The results show that pse(t) is sensitive to high temperature. The probable function of PSE(T) is discussed. 相似文献
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Hui Li Mei-hua Sun Ming-fang Qi Jiao Xing Tao Xu Han-ting Liu Tian-lai Li 《Journal of Zhejiang University. Science. B》2018,19(6):445-457
Tomato is an ideal model species for fleshy fruit development research. SlYABBY2b regulates the ovary locule number, which is increased by gibberellins, in tomato. However, the relationship between SlYABBY2b and endogenous gibberellin is poorly understood. In this study, SlYABBY2b-overexpressing and RNA interference (RNAi) transgenic tomato plants were used to elucidate the mechanism by which SlYABBY2b regulates the ovary locule number and endogenous gibberellin content in tomato. SlYABBY2b-overexpressing plants showed fewer locules and lower gibberellin content than the control plants. Contrasting results were found in the RNAi lines. Therefore, the SlYABBY2b gene negatively regulates tomato ovary locule number and endogenous gibberellin content. Furthermore, the expression of SlYABBY2b gene was remarkably higher than that of the wild type in the apical shoots of gibberellindeficient mutants. This showed that the gibberellins can inhibit the expression of SlYABBY2b gene negative regulation. Further study revealed that SlYABBY2b suppressed the expression of SlGA20ox1 and SlGA3ox2, but increased that of SlGA2ox1 and SlGA2ox5 in the apical shoots of SlYABBY2b-overexpressing plants, thereby reducing gibberellin content. Contrasting results were found in the RNAi lines. Our results showed that the SlYABBY2b gene was located on gibberellin signal transduction pathways, fed back regulation of the synthesis of gibberellin, and felt exogenous gibberellin signal to further regulate the formation of tomato locule. 相似文献
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Lang QL Zhou XC Zhang XL Drabek R Zuo ZX Ren YL Li TB Chen JS Gao XL 《Journal of Zhejiang University. Science. B》2011,12(2):116-125
A large number of plant microRNAs (miRNAs) are now documented in the miRBase, among which only 30 are for Solanum lycopersicum (tomato). Clearly, there is a far-reaching need to identify and profile the expression of miRNAs in this important crop under
various physiological and pathological conditions. In this study, we used an in situ synthesized custom microarray of plant
miRNAs to examine the expression and temporal presence of miRNAs in the leaves of tomato plants infected with Cucumber mosaic virus (CMV). Following computational sequence homology search and hairpin structure prediction, we identified three novel tomato
miRNA precursor genes. Our results also show that, in accordance with the phenotype of the developing leaves, the tomato miRNAs
are differentially expressed at different stages of plant development and that CMV infection can induce or suppress the expression
of miRNAs as well as up-regulate some star miRNAs (miRNA*s) which are normally present at much lower levels. The results indicate
that developmental anomalies elicited by virus infection may be caused by more complex biological processes. 相似文献
15.
The effect of crying on long-term memory in infancy 总被引：1，自引：0，他引：1
The influence of crying on infants' long-term memory for a learned response was investigated in 3 experiments. In each, infants were trained to move a crib mobile containing 10 identical objects by means of kicking and were then exposed to a reinforcer containing only 2 of these components. This shift in component numerosity produced crying in 53% of the infants. Infants who cried in response to the reward shift evidenced no retention of the contingency 1 week later (Experiment 1) but did have excellent retention at 1 day (Experiment 2). In Experiment 3, a brief reactivation treatment alleviated forgetting at 3 weeks regardless of the presence of crying in response to the change in mobiles. An unexpected recency effect characterized the efficacy of the reactivation treatment. The results indicate that crying in response to the violation of a reward-expectation habit functions as an amnesic agent to produce accelerated forgetting. 相似文献
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研究了NaCl胁迫对5个不同番茄品种的幼苗生长、果实可溶性糖含量及产量的影响.结果表明,盐胁迫抑制了番茄幼苗植株的生长,降低了幼苗的单株鲜重,减少了果实产量,但可以提高果实可溶性糖含量.同一盐胁迫条件下,不同番茄品种性状存在显著差异. 相似文献
17.
Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and
inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5’NC region and C region,
were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt. CD-SR (, containing either HCRZNC or HCRZC were constructued
and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hydridization were used to determine
the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%,
respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion:
Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better
effect when used together. 相似文献
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A hydroponic experiment carried out to study the effect of five Cd levels on growth and photosynthesis of two tomato cultivars showed that the addition of 0.1 μmol/L Cd induced a slight increase in plant height of Hezuo 903 and the SPAD (the Soil-Plant Analyses Development) value of the 2 cultivars. However, at higher Cd levels, i.e., 1 and 10 μmol/L, root length and volume, plant height, and SPAD value were all significantly reduced. On an average of the 2 cultivars, exposure to 1 and 10μmol/L Cd for 33 d reduced plant height by 18.9% and 46.4% and SPAD value by 11.2% and 31.6%, compared with control,respectively. Similarly, root length was reduced by 41.1% and 25.8% and root volume by 45.2% and 63.7%, respectively. The addition of Cd in the growth medium also had significant deleterious effect on net photosynthetic rate (Pn) and intracellular CO2concentration (Ci), with Pn being reduced by 27.2% and 62.1% at 1 μmol/L and 10 μmol/L Cd treatments compared to the control,respectively, while Ci increased correspondingly by 28.4% and 39.3%. 相似文献
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