共查询到19条相似文献，搜索用时 171 毫秒
1.
目的:探讨尿β2-微球蛋白(β2-MG)、尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)和血清胱抑素C(Cys C)三项肾功能指标联合检测对高血压肾损害早期的临床诊断价值。方法:将106例高血压患者以微量蛋白尿呈阴性、阳性分为A、B两组,A组56例,B组50例,另选择56名健康体检者作为对照组,分别检测血清肌酐(Scr)、血清Cys C、尿β2-MG和尿NAG四项指标。结果:三组Scr检测值比较无显著性差异(P>0.05);A组和B组Cys C、β2-MG、NAG三项检测值均明显高于对照组(P<0.01),而B组又明显高于A组(P<0.01)。Cys C、β2-MG、NAG三项指标联检对高血压早期肾损害的阳性诊断率达87.7%,明显高于单项检测及二项联检阳性率(P<0.01)。结论:Cys C、β2-MG、NAG三项指标联检能较早地反映高血压肾损害程度,对高血压早期肾损害的诊断具较高的敏感性。 相似文献
2.
目的:观测尾加压素Ⅱ受体拮抗剂palosuran对阿霉素肾病大鼠尿中尾加压素Ⅱ(UrotensinⅡ;UⅡ)的影响,从而探究palosuran对微小病变肾病综合征(minimal change ne-phropathy syndrome;MCNS)的作用。方法:采用雄性Wistar大鼠80只,体重100±20g。随机选择其中10只做为健康组,其余70只大鼠尾静脉一次性注射阿霉素5mg/kg建立动物模型。注射后第7天检测大鼠24小时尿蛋白定量,若大鼠尿蛋白>30mg/24h者为MCNS模型成功,不合格者予以剔除。将合格的大鼠,随机分成2组,一组设为微小病变型肾病模型组,另一组在微小病变型肾病模型的基础上采用palosuran混入饲料中给药方法喂养,设为干预组。在实验第14、21、28天检测各组大鼠采用放射免疫法检测各组大鼠尿UⅡ的水平。结果:健康组大鼠尿UⅡ第14、21、28天检测值分别是1.46±0.18 ng/mL,1.52±0.12 ng/mL,1.54±0.43 ng/mL;模型组分别是2.49±0.11 ng/mL,4.48±0.19 ng/mL,6.56±0.27 ng/mL;干预组分别是1.95±0.1ng/mL,3.31±0.15 ng/mL,2.58±0.21 ng/mL。随着时间的延续,模型组大鼠尿UⅡ进行性升高(P<0.05),而palosuran对肾病大鼠尿UⅡ水平有明显影响,差异有显著统计意义(P<0.05)。结论:palosuran可能通过抑制UⅡ的作用而对微小病变肾病大鼠肾损害产生保护作用。 相似文献
3.
熊友谊 《安徽科技学院学报》2016,50(2):27-31
目的:研究三七总皂苷联合氯吡格雷对高血脂症血液流变学的作用。方法:大鼠随机分为5组:正常对照组、模型组、三七总皂苷组、氯吡格雷组、三七总皂苷组联合氯吡格雷组(PNSBGL)。除正常对照组外,其余各组喂饲高脂饲料,每日1次,连续给8周;自制模之日起,治疗组分别给予三七总皂苷、氯吡格雷、三七总皂苷+氯吡格雷治疗。8周后采血,测定各组小鼠全血粘度、血小板功能和血脂水平。结果:与模型组比较,三七总皂苷、PNSBGL治疗组血粘度、血小板功能、血脂含量、纤维蛋白原含量、红细胞压积呈现显著性降低(P0.05,P0.01或P0.001),氯吡格雷组血粘度、血小板功能、红细胞压积呈现显著性降低(P0.01或P0.001);与三七总皂苷组比较,PNSBGL治疗组血粘度、血小板功能、红细胞压积呈现显著性降低(P0.05或P0.01);与氯吡格雷组比较,PNSBGL治疗组血粘度、血小板功能、血脂含量、纤维蛋白原含量、红细胞压积呈现显著性降低(P0.05,P0.01或P0.001)。结论:三七总皂苷与氯吡格雷联合应用对高脂血症大鼠血液流变学改变具有协同改善作用。 相似文献
4.
自发性高血压大鼠血管平滑肌CaN与心肌ATP酶活性变化 总被引：2，自引：0，他引：2
目的:研究自发性高血压大鼠不同血管平滑肌钙调神经磷酸酶(CaN)的活性与心肌ATP酶活性变化.方法:应用酶活性测定12周自发性高血压大鼠(SHR)和Wistar大鼠的胸主动脉(A)、肠系膜动脉(MA)、尾动脉(CA)血管平滑肌CaN及其心肌Na -K -ATPase的活性.结果:SHR大鼠不同血管CaN的活性与对照组相应的变化无显著差异,而其在CA中的活性明显低于在A的活性,在阻力血管MA中活性却无明显降低;Na -K -ATPase的活性与其对照组相应的变化有显著差异(P<0.01) ;SHR大鼠血压和左心室肥厚指数增加(P<0.05).结论:SHR大鼠血压和左心室肥厚指数增加,SHR大鼠血压与其对照组相比不同血管平滑肌CaN的活性变化无显著差异,Na -K -ATPase的活性发生下降. 相似文献
5.
糖化血红蛋白和尿微量蛋白检测的临床意义 总被引：1，自引：0，他引：1
目的：探讨1型糖尿病患者糖化血红蛋白（HbA1c）以及随机尿中尿微量蛋白（β2-MG、mAlb）的检测对发现1型糖尿病早期肾脏损害的临床价值。方法：糖化血红蛋白（HbAlc）用胶乳凝集反应法，尿液微量蛋白（β2-MG、mAlb）用放免法进行测定。结果：HbAlc和尿β2-MG、mAlb在尿蛋白阴性组、尿蛋白阳性组和正常对照组之间差异有显著性（P〈0．01）。结论：尿β2-MG、mAlb和HbAlc的检测可作为诊断1型糖尿病早期肾损害敏感而又可靠的常规指标。 相似文献
6.
目的：探讨血清γ-痕迹蛋白、尿N-乙酰-B-D-氨基葡萄糖苷酶（NAG）与尿微量白蛋白检测对原发性高血压早期肾损害的临床意义。方法：将86例高血压病患者按有无肾脏损害分为两组,原发性高血压肾损害者48例,单纯性高血压患者38例,同时选择54例健康体检人群作为正常对照组。分别测定血清γ-痕迹蛋白、尿NAG、尿微量白蛋白并对结果进行比较分析。结果：单纯性高血压组以上检测项目结果与正常对照组比较差异无显著性（P〉0．05）;高血压肾损害组各检测结果与对照组比较差异有显著性（P〈0．01）。结论：血清γ-痕迹蛋白、尿NAG与尿微量白蛋白联合检测对原发性高血压早期肾损害的诊断有重要意义。 相似文献
7.
目的:观察人参皂苷对D-半乳糖(D-gal)致衰老大鼠学习记忆和脑单胺类神经递质的影响。方法:成年雄性SD大鼠随机分为正常组,衰老组,人参皂苷低(50 mg/kg)、高剂量组(100 mg/kg),每组10只,制备D-gal亚急性衰老大鼠模型,采用自主活动仪记录法、Morris水迷宫法、穿梭箱法检测各组大鼠的自主活动和空间学习记忆能力;采用高效液相色谱-电化学法检测各组大鼠海马、下丘脑单胺类神经递质去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)含量。结果:与正常组比较,模型组干预后大鼠自主活动次数显著减少(P<0.01),大鼠水迷宫探索路径、搜台潜伏期、平均潜伏期、进入错误区及电击时间延长(P<0.01),遭受电击次数显著增加(P<0.01),海马及下丘脑NE、DA、5-HT含量均显著降低(P<0.01)。与模型组比较,人参皂苷低、高剂量组大鼠自主活动次数明显增加(P<0.05,P<0.01),探索路径和搜台潜伏期、平均潜伏期、进入错误区及电击时间显著缩短(P<0.05,P<0.01),遭受电击次数明显减少(P<0.05,P<0.01),海马及下丘脑NE、DA、5-HT含量均明显升高。结论:人参皂苷具有改善衰老大鼠的空间记忆能力,提高大鼠脑组织中单胺类神经递质含量,进而延缓脑衰老进程的作用。 相似文献
8.
宋祖荣 《赤峰学院学报(自然科学版)》2015,(8)
目的：探析益气活血方对早期糖尿病肾病大鼠肾脏PKC、TGF-β1及血清Chol、TG等指标的影响。方法：选择糖尿病肾病造模成功的64只SD大鼠,随机分为空白对照组(11只)、依那普利组11只、益气活血方大剂量组(观察组1,14只)、益气活血方中剂量组(观察组2,14只)、益气活血方小剂量组(观察组3,14只),并选择11只健康SD大鼠作为正常组。比较与分析各组大鼠的血清TG、Chol水平以及大鼠肾组织PKC、TGF-β1指标表达情况。结果：DN空白对照组大鼠的血清TG、Chol水平、肾组织PKC、TGF-β1表达均明显高于正常组、依那普利组以及益气活血方组。其中,益气活血方组的三个剂量组均可显著降低血清TG、Chol水平、肾组织PKC、TGF-β1表达等,尤以大剂量组的效果最优。结论：益气活血方可以有效缓解肾小球纤维化病变,从而有效保护肾脏。 相似文献
9.
王念辉 《临沂师范学院学报》2009,31(3):113-116
为探讨辅酶Q10对大强度耐力训练大鼠运动能力及疲劳恢复的影响,选用SD雄性健康大鼠32只,按完全随机原则分配到安静组、安静力竭组、运动力竭组和运动加药力竭组,每组各8只;建立大鼠大强度耐力跑台训练模型,检测大鼠血清Hb,Glu,Bla,LDH,CK和BUN等指标．结果表明：CoQ10能有效抑制运动训练后大鼠Hb含量下降;显著降低大强度耐力训练后大鼠血清LDH,CK等血清酶活性的升高和运动训练后BUN和Bla含量的升高． 相似文献
10.
人参皂苷Rg1对糖尿病大鼠心肌氧化应激及细胞凋亡的影响（英文） 总被引：2，自引：0，他引：2
Hai-tao YU Juan ZHEN Bo PANG Jin-ning GU Sui-sheng WU 《Journal of Zhejiang University. Science. B》2015,16(5):344-354
目的:探讨人参皂苷Rg1对高血糖所致心肌损害的保护作用及其机制。创新点:使用糖尿病大鼠为实验对象,探讨三种浓度的人参皂苷Rg1对糖尿病心肌损伤的保护作用及其机制,检测其是否具有浓度依赖性。方法:将60只Wistar大鼠随机分组,其中空白对照组10只,另50只给予高脂高糖饲养,4周后腹腔注射40 mg/kg链脲佐菌素(STZ)。成功制备糖尿病大鼠模型40只,再随机分为糖尿病模型组,糖尿病大鼠+低剂量人参皂苷Rg1(10 mg/(kg·d)),糖尿病大鼠+中剂量人参皂苷Rg1(15 mg/(kg·d)),糖尿病大鼠+高剂量人参皂苷Rg1(20 mg/(kg·d))。12周后处死大鼠,取血测定空腹血糖、总胆固醇(TC)、甘油三酯(TG)、心肌酶及氧化应激水平,留取心肌组织使用透射电镜观察心肌细胞超微结构改变,应用TUNEL法检测心肌细胞凋亡,免疫组化检测细胞凋亡相关蛋白半胱氨酸天冬氨酸蛋白酶3(CASP3)和Bcl-x L的表达。结论:人参皂苷Rg1对糖尿病大鼠糖脂代谢无明显影响,人参皂苷Rg1可降低糖尿病大鼠血清肌钙蛋白(c Tn I)和肌酸激酶同工酶(CK-MB)水平,改善心肌细胞超微结构,减少心肌细胞凋亡,降低大鼠血清和心肌组织中丙二醛(MDA)含量,提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH)水平,降低凋亡蛋白CASP3的表达,同时提高Bcl-x L蛋白表达。总之,人参皂苷Rg1能显著保护糖尿病大鼠心肌损伤,其机制可能与其抗氧化及抗细胞凋亡作用有关。 相似文献
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12.
本文主要探讨唾液与血清中BUN、Cr、UA的相关性和临床应用价值。住院肾脏病患者73例和健康人42例,检测血清和唾液中的尿素氮、肌酐及尿酸浓度。肾脏病患者唾液和血清BUN、Cr、UA有较好的相关性。肾脏病患者唾液BUN、Cr、UA含量均比健康人高。健康人唾液和血清BUN有较好的相关性,Cr无相关性。唾液UA浓度随血清UA浓度的增加而升高。唾液中BUN、Cr、UA可用于判定肾脏功能障碍病人的疾病损害程度。 相似文献
13.
Li-jiang Tang Jian-jun Jiang Xiao-feng Chen Jian-an Wang Xian-fang Lin Yu-xi Du Cong-feng Fang Zhao-xia Pu 《Journal of Zhejiang University. Science. B》2010,11(8):592-598
Objective:Uric acid(UA) is considered to be a powerful predictor of cardiovascular risk and hyperuricemia might be involved in the metabolic syndrome(MS).This study aims to investigate the relation between UA levels and aortic root dilatation.Methods:A total of 348 hypertensive patients [age(67.5±9.8) years] with or without MS were included in the study.The aortic root diameters at the aortic annulus,the sinuses of Valsalva,the sinotubular junction,and the proximal part of the ascending aorta were measured using a two-dimensional(2D) echocardiography.Serum UA levels were also measured for all patients.Results:A high UA level is independently associated with aortic root diameters at the sinuses of Valsalva(P=0.001) and the proximal ascending aorta(P0.0001) in the hypertensive patients without MS.In contrast,aortic root diameters were not significantly related to UA levels in the hypertensive patients with MS.Furthermore,increased UA levels were associated with an increased risk for aortic root dilatation in the patients without MS(sex-adjusted hazard ratio 1.75,95% confidence intervals(CI) 1.27-2.41),but not in those with MS.Conclusions:This study demonstrated an independent relationship between the aortic root dimensions and increased levels of serum UA in the hypertensive patients without MS.Further understanding of the mechanisms underlying these associations may allow a clearer interpretation of the potential value of specific urate-lowering treatment on cardiovascular disease. 相似文献
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Sheng-chun DANG Yan-hua ZENG Ping-jiang WANG Bao-ding CHEN Rong-fang CHEN Arun KUMAR SINGH Pankaj KUMAR Shu FENG Lei CUI Hao WANG Jian-xin ZHANG 《Journal of Zhejiang University. Science. B》2014,15(6):556-565
Background and objective
It has been shown that macrophages play an important role in the development of severe acute pancreatitis (SAP), and eventually lead to multiple organ failure (MOF). Clodronate-liposome selectively depleted macrophages. This study was to investigate the role of renal macrophage infiltration in acute renal injury in rats with SAP and to evaluate the potential of superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance imaging (MRI) for diagnosis.Methods
Superparamagnetic Fe3O4 nanoparticles were prepared by chemical coprecipitation. SPIO-liposomes and SPIO-clodronate-liposomes were prepared by the thin film method. SAP models were prepared by injection of sodium taurocholate into the subcapsular space of rat pancreas. Sprague-Dawley rats were randomly divided into a control group, SAP plus SPIO-liposome (P) group, and SAP plus SPIO-clodronate-containing liposome (T) group. Kidney injury was evaluated by T2-weighted MRI scan. The levels of serum amylase (SAM), blood urea nitrogen (BUN), and serum creatinine (SCr) were measured by an automated enzymatic method. Serum tumor necrosis factor-α (TNF-α) was measured by enzyme-linked immunosorbent assay (ELISA). Pathological changes in the pancreas and kidney were observed using hematoxylin and eosin (H&E) staining, while cell apoptosis was detected with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. In addition, the macrophage markers (CD68) of the renal tissue were detected with immunohistochemistry.Results
The pathological changes in the pancreas and kidneys of rats in the T group were milder than those in the P group. The MRI signal intensity of the kidneys in the P and T groups was significantly lower than that in the control group. There were significant changes in the two experimental groups (P<0.01). The levels of SAM, Bun, SCr, and TNF-α in rats in the P group were higher than those in the control group (P<0.01) and in the T group (P<0.01). The apoptosis of the kidney in the T group was higher than that in the P group at 2 and 6 h (P<0.01).Conclusions
Clodronate-containing liposomes protected against renal injury in SAP rats, and SPIO can be used as a tracer for MRI examination to detect renal injury in SAP rats. SPIO-aided MRI provided an efficient non-invasive way to monitor the migration of macrophages after renal injury in rats with SAP. 相似文献15.
目的：观察奥扎格雷钠与黄芪注射液联合治疗糖尿病肾病的疗效。方法：将84例早期糖尿病肾病患者随机分为3组，每组28例，在常规治疗的基础上，A组加用黄芪注射液，B组加用奥扎格雷钠注射液，C组加用黄芪注射液与奥扎格雷钠注射液，疗程均为20d。观察治疗前后各组尿白蛋白排泄率（UAER），血尿素氮（BUN），血清肌酐（SCr），糖化血红蛋白（HbAlc）的变化。结果：糖化血红蛋白，血清肌酐，血尿素氮，尿白蛋白排泄取：3组治疗后均比治疗前显著下降（P〈0．01），C组下降幅度优于A组和B组（P〈0．05）。结论：奥扎格雷钠联合黄芪注射液治疗糖尿病肾病能降低糖化血红蛋白，显著降低尿白蛋白排泄率，同时改善肾功能。 相似文献
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目的:观察川芎嗪治疗实验性免疫性肾损伤作用和机理,为中药治疗肾小球肾炎开辟新途径。方法:猪血清腹腔注射8周,复制大鼠实验性肾损伤模型,川芎嗪治疗4周后处死动物,检测尿蛋白、血清BUN和Scr含量,取肾组织制作光、电镜标本检查。结果:川芎嗪大、小剂量组尿蛋白、血清BUN和Scr含量较模型组明显降低(P<0.01),肾小球的病理形态学改变较模型组轻。结论:川芎嗪具有治疗大鼠实验性免疫性肾损伤的作用。 相似文献
17.
Sheng-hong ZHU ;Li-jia ZHOU ;Hong JIANG ;Rong-jun CHEN ;Chuan LIN ;Shi FENG ;Juan JIN ;Jiang-hua CHEN ;Jian-yong WU 《Journal of Zhejiang University. Science. B》2014,15(8):735-742
研究目的:在小鼠模型中利用吲哚美辛阻断COX-1/2通路,探讨非甾体类抗炎药对肾缺血再灌注损伤的保护作用。创新要点:非甾体类抗炎药被认为具有肾毒性,本研究首次在小鼠模型中探讨非甾体类抗炎药对肾缺血再灌注损伤的保护作用。研究方法:小鼠左侧肾蒂夹闭后,通过腹腔注射不同剂量的吲哚美辛,在肾缺血再灌注损伤24小时后,获取血液和肾脏标本。利用酶联免疫(ELISA)试剂盒测定血清肌酐和细胞因子浓度来评估肾功能,肾组织样本进行苏木精-伊红染色和免疫组化分析。重要结论:腹腔注射吲哚美辛5 mg/kg组的小鼠血清肌酐值与对照组相比显著降低,肾小管损伤也显著减轻(见图1和2);腹腔注射5和7 mg/kg吲哚美辛组的小鼠血清肾肿瘤坏死因子-α和白介素-6的浓度显著降低(见图3a和3b);腹腔注射3和5 mg/kg吲哚美辛组的小鼠血清血栓素B2与6-酮前列腺素F1α的比值明显降低(见图3c);腹腔注射5 mg/kg吲哚美辛组小鼠肾组织COX-1和COX-2染色较弱(见图4)。因此,吲哚美辛对小鼠肾缺血再灌注损伤的作用与其剂量相关,在某个特定的剂量范围内具有肾保护作用。吲哚美辛对小鼠肾缺血再灌注损伤的保护作用与阻断COX-1/2有关。 相似文献
18.
目的探讨血清胱抑素C（Cystainc）水平在原发性高血压早期肾损伤临床诊断中的价值。方法高血压病1。2级患者共41例作为观察组,同时选择41例健康体检者作为对照组,分别检测两组治疗前后Cystain C、尿微量白蛋白（mAlb）、血清肌酐（Scr）、尿素氮（BUN）、内生肌酐清除率（Ccr）等。结果观察组与对照组间Cystain C、24hmAlb、Ccr有显著性差异（P〈0．01）、Scr差异无显著性（P〉0．05）;结论血清Cystain C的浓度比BUN、Scr浓度更能够反映肾小球滤过功能的损害,是早期诊断肾小球滤过功能受损的敏感指标之一。 相似文献
19.
Liao F Zhao YS Zhao LN Tao J Zhu XY Liu L 《Journal of Zhejiang University. Science. B》2006,7(6):497-502
A patented kinetic uricase method was evaluated for serum uric acid assay. Initial absorbance of the reaction mixture before uricase action （A0） was obtained by correcting the absorbance at 293 nm measured before the addition of uricase solution, and background absorbance （Ab） was predicted by an integrated method. Uric acid concentration in reaction solution was calculated from AA, the difference between A0 and Ab, using the absorptivity preset for uric acid. This kinetic uricase method exhibited CV〈4.3% and recovery of 100%. Lipids, bilirubin, hemoglobin, ascorbic acid, reduced glutathione and xanthine 〈0.32 mmol/L in serum had no significant effects. △A linearly responded to 1.2 to 37.5 μmol/L uric acid in reaction solution containing 15 μl serum. The slope of linear response was consistent with the absorptivity preset for uric acid while the intercept was consistent with that for serum alone. Uric acid concentrations in clinic sera by different uricase methods positively correlated to each other. By Bland-Altman analysis, this kinetic uricase method accorded with that by quantifying the total change of UV absorbance on the completion of uricase reaction. These results demonstrated that this kinetic uricase method is reliable for serum uric acid assay with enhanced resistance to both xanthine and other common errors, wider range of linear response and much lower cost. 相似文献