Single cell kinase signaling assay using pinched flow coupled droplet microfluidics     

Ramesh Ramji  Ming Wang  Ali Asgar S. Bhagat  Daniel Tan Shao Weng  Nitish V. Thakor  Chwee Teck Lim  Chia-Hung Chen 《Biomicrofluidics》2014,8(3)

Droplet-based microfluidics has shown potential in high throughput single cell assays by encapsulating individual cells in water-in-oil emulsions. Ordering cells in a micro-channel is necessary to encapsulate individual cells into droplets further enhancing the assay efficiency. This is typically limited due to the difficulty of preparing high-density cell solutions and maintaining them without cell aggregation in long channels (>5 cm). In this study, we developed a short pinched flow channel (5 mm) to separate cell aggregates and to form a uniform cell distribution in a droplet-generating platform that encapsulated single cells with >55% encapsulation efficiency beating Poisson encapsulation statistics. Using this platform and commercially available Sox substrates (8-hydroxy-5-(N,N-dimethylsulfonamido)-2-methylquinoline), we have demonstrated a high throughput dynamic single cell signaling assay to measure the activity of receptor tyrosine kinases (RTKs) in lung cancer cells triggered by cell surface ligand binding. The phosphorylation of the substrates resulted in fluorescent emission, showing a sigmoidal increase over a 12 h period. The result exhibited a heterogeneous signaling rate in individual cells and showed various levels of drug resistance when treated with the tyrosine kinase inhibitor, gefitinib.  相似文献   

Quantifying spatio-temporal dynamics of biomarker pre-concentration and depletion in microfluidic systems by intensity threshold analysis     

Ali Rohani  Walter Varhue  Yi-Hsuan Su  Nathan S. Swami 《Biomicrofluidics》2014,8(5)

  相似文献   

Additive Effect of Lipid Lowering Drug (Simvastatin) in Combination with Antidiabetic Drug (Glibenclamide) on Alloxan Induced Diabetic Rats with Long Term Dyslipidemia     

Mst. Marium Begum  Zakia Sultana  Md. Ershad Ali  Md. Safkath Ibne Jami  Proma Khondkar  Md. Masuduzzaman Khan  Md. Mominul Haque 《Indian journal of clinical biochemistry : IJCB》2014,29(4):452-461

High blood glucose level, elevated level of liver enzyme, necrosis and shrinkage of islets of Langerhans has been implicated in the pathogenesis of type 2 diabetes. High blood glucose cause oxidative stress, production of free radical as well as elevated SGPT and SGOT level. Both glibenclamide and simvastatin in fixed dose used as antihyperglycemic antidyslipidemic and antioxidative agents for type 2 diabetes treatment. This study therefore aimed to evaluate the antihyperglycemic, antidyslipidemic and antioxidative effect of fixed dose combination of glibenclamide (0.6 mg/70 kg body weight) and simvastatin (5 mg/70 kg body weight) on long term alloxan induced diabetic rats with cardiovascular disease using various diagnostic kits as a parameter of phamacotherapeutic and pharmacological effect. The study was carried out using 96 Swiss Albino male rats weighing about 200–220 g. Combination therapy induced a significant decrease in blood glucose level in alloxan induced diabetic rats, from 33.75 ± 1.65 to 5.80 ± 0.07 mmol/l 2 h after last dose administration, after 4 weeks treatment. In case of dyslipidemic effect, combination therapy reduced total cholesterol (45 %), triglyceride (36 %) and low density lipoprotein-cholesterol (32 %) levels significantly and increased high density lipoprotein-cholesterol level (57 %) in comparison with their respective diabetic control groups. Results of this study showed that combination therapy effectively decreased SGPT (ALAT) (55 %) and SGOT (ASAT) (51 %) in comparison with diabetic control group. It was also observed that catalase and superoxide dismutase enzyme activity was increased by 58 and 91 % respectively in comparison with diabetic control group after 4 weeks treatment with combination of both drugs. In conclusion, these findings of combination therapy (glibenclamide and simvastatin) on alloxan induced diabetes in rats are significantly better than monotherapy using single drug. The results of the present study suggest that, combination of the fixed dose of glibenclamide and simvastatin might be efficacious in patients with diabetic dyslipidemia and increased oxidative stress. Furthermore, this combination therapy offer dosage convenience to the patients and by virtue of its dual mode of action might be a useful addition to the therapeutic armamentarium for patients with diabetic dyslipidemia and oxidative stress.  相似文献   

Determinants of students’ outcome: a full-fledged structural equation modelling approach     

Mohammed Borhandden Musah  Hairuddin Bin Mohd Ali  Shafeeq Hussain Vazhathodi Al-Hudawi  Lokman Mohd Tahir  Khadijah Binti Daud  Abdul Rahim Hamdan 《Asia Pacific Education Review》2015,16(4):579-589

  相似文献   

Corrigendum to “Robust FOPID stabilization of retarded type fractional order plants with interval uncertainties and interval time delay” [Journal of the Franklin Institute Volume 356, Issue 16, 2019, Pages 9302–9329]     

Majid Ghorbani  Mahsan Tavakoli-Kakhki  Ali Akbar Estarami 《Journal of The Franklin Institute》2021,358(2):1692

  相似文献   

Scale-free collaborative protocol design for state and regulated state synchronization of multi-agent systems with arbitrary fast convergence     

Zhenwei Liu  Ali Saberi  Anton A. Stoorvogel  Donya Nojavanzadeh 《Journal of The Franklin Institute》2021,358(9):4864-4882

In this paper, we design scale-free collaborative protocols for state and regulated state synchronization of homogeneous multi-agent systems (MAS) with arbitrary fast convergence. The protocol design solely depends on the knowledge of the agents’ model and does not require any information about the communication network and the number of agents. Moreover, our protocols can achieve synchronization with any desired convergence rate by simply tuning a design parameter.  相似文献   

From information seeking to information avoidance: Understanding the health information behavior during a global health crisis     

Saira Hanif Soroya  Ali Farooq  Khalid Mahmood  Jouni Isoaho  Shan-e Zara 《Information processing & management》2021,58(2):102440

Individuals seek information for informed decision-making, and they consult a variety of information sources nowadays. However, studies show that information from multiple sources can lead to information overload, which then creates negative psychological and behavioral responses. Drawing on the Stimulus-Organism-Response (S-O-R) framework, we propose a model to understand the effect of information seeking, information sources, and information overload (Stimuli) on information anxiety (psychological organism), and consequent behavioral response, information avoidance during the global health crisis (COVID-19). The proposed model was tested using partial least square structural equation modeling (PLS-SEM) for which data were collected from 321 Finnish adults using an online survey. People found to seek information from traditional sources such as mass media, print media, and online sources such as official websites and websites of newspapers and forums. Social media and personal networks were not the preferred sources. On the other hand, among different information sources, social media exposure has a significant relationship with information overload as well as information anxiety. Besides, information overload also predicted information anxiety, which further resulted in information avoidance.  相似文献   

Acceptance of mobile technologies and M-learning by university students: An empirical investigation in higher education     

Al-Rahmi  Ali Mugahed  Al-Rahmi  Waleed Mugahed  Alturki  Uthman  Aldraiweesh  Ahmed  Almutairy  Sultan  Al-Adwan  Ahmad Samed 《Education and Information Technologies》2022,27(6):7805-7826

Education and Information Technologies - Mobile-learning (M-learning) apps have grown in popularity and demand in recent years and have become a typical occurrence in modern educational systems,...  相似文献   

Minimization of students’ cognitive load in a virtual chemistry laboratory via contents optimization and arrow-textual aids     

Ali  Numan  Ullah  Sehat  Khan  Dawar 《Education and Information Technologies》2022,27(6):7629-7652

Education and Information Technologies - Virtual Chemistry Laboratories (VCLs) are used as an alternative to the physical laboratories, where users can enhance their performance for hands-on...  相似文献   

Training Transfer and Transfer Motivation: The Influence of Individual,Environmental, Situational,Training Design,and Affective Reaction Factors     

Muhammad Awais Bhatti  Sharrifah Ali  Mohd Faizal Mohd Isa  Mohamed Mohamed Battour 《Performance Improvement Quarterly》2014,27(1):51-82

The purpose of this study is to examine the effects of individual, environmental, training design, and affective reaction factors on training transfer and transfer motivation. To determine the relationship between these factors and their influence on training transfer and to test the model, the researchers collected data from employees in the Malaysian banking sector. Structural equation modeling with Amos 16 was used to test the model and determine the relationship. The study suggested that training stakeholders should manage the training program effectively. Transfer is maximized when trainees have social support, high performance self‐efficacy, and transfer motivation. Stakeholders (e.g., trainers, trainees, supervisors, and peers) are important to the training transfer process, as are learner readiness, trainee reaction, instrumentality, and training retention. This study revealed that perceived content validity and transfer design work together and influence the trainee's performance self‐efficacy. In other words, if trainers want to improve the performance self‐efficacy level of trainees, they need to explain how the trainee can transfer the learned skills at the workplace and make sure the content of the training is similar to the actual job. The main objective of training programs is to align the employee's expertise with organizational goals. Organizations can achieve their desired objectives only when employees transfer the learned skills on the job. Unfortunately, employees often transfer only a small percentage of skills they have learned in training. To effectively manage their training programs, organizations need to identify and focus on the factors that resist effective training transfer.  相似文献