Molecular mechanism of pathogenesis of dengue virus: Entry and fusion with target cell     

Seema  S. K. Jain 《Indian journal of clinical biochemistry : IJCB》2005,20(2):92-103

Dengue fever is one of the major health problems in India. Interaction with specific receptor(s) at the cell surface is one of the first events in the pathogenesis of Dengue virus. However, relatively little is known about these receptors. Cellular receptors in human monocytes and mouse neural cells are main target for the viral infection. The envelope protein of the virus (E-protein) plays important role in attachment of virus to target cells and their interaction with cellular receptors. The modulation of receptor gene(s) and/or protein(s) can be used as a method for interfering with virus entry and can thus become a new method for disease prevention. The receptors can be purified by affinity chromatography using E-protein as ligand. It has been reported that addition of highly sulfated heparan sulfate prevents E-protein binding to target cells suggesting that heparan sulfate is utilized by dengue envelope protein to bind to target cells.  相似文献   

基于知识生成的工作场所学习     

程薇  杨现民  余胜泉 《现代远程教育研究》2013,(4):80-87,100

工作场所学习作为一种有别于传统"学校本位教育"的学习方式,发生在工作场所之中,其内涵经历了知识技能观、交互观、过程观和混合观四个阶段的发展演化。伴随新媒体技术在工作场所中的应用与普及,工作场所学习的内涵发生了新的变化:工作学习一体化、集体知识群建共享、工作学习内容生成而非预设等。知识生成视角下的工作场所学习是工作场所学习的高级形式,它的提出是以知识生成隐喻为基础,强调集体知识库的生成、虚实结合的协作交流与分享、学习者之间的交流与互动。学习元平台作为一种能够支持协同创建、内容生成以及语义关联的知识社区,可以支持虚实结合的工作场所学习方式,实现知识与技能获得、参与工作任务、知识系统化生成三个阶段的学习。  相似文献   

拳卷地钱总黄酮提取物抗乙型肝炎病毒体外实验研究     

朱华  梁东艳  笪舫芳 《大众科技》2013,(4):110-111,99

目的:通过体外实验,探讨拳卷地钱总黄酮提取物抗乙肝病毒的作用。方法:将拳卷地钱总黄酮提取物作用于HBV-DNA转染细胞系2.2.15细胞,通过检测细胞培养液中HBsAg、HBeAg的变化来评价拳卷地钱总黄酮提取物抗乙肝病毒的效果及其可能的药理活性部位。结果:当拳卷地钱总黄酮提取物的最大无毒浓度为31.3μg·mL-1,对2.2.15细胞HBeAg、HBsAg分泌抑制率分别为51.56%、39.69%;治疗指数(TI)>2。结论:在2.2.15细胞无毒浓度下,拳卷地钱总黄酮对2.2.15细胞HBsAg、HBeAg分泌具有比较好的抑制作用;且可以有效阻止HBV-DNA表达和复制。提示:拳卷地钱总黄酮提取物在体外有一定的抗乙肝病毒作用。  相似文献   

Construction of a eukaryotic expression vector pEGFP-C1-BMP-2 and its effect on cell migration     

Xiao-ying Wang  Zhong-hua Chen  Ru-yi Zhang  Sen-quan Liu  Zhu Mei  Ying-ying Yu  Xiong Zhang  Qiang Xia  Yue-min Ding 《Journal of Zhejiang University. Science. B》2012,13(5):356-363

Objective  

Bone morphogenetic proteins (BMPs) are known to play an important role in bone and cartilage development. Recent research has shown that BMPs may induce tumorigenesis and promote tumor to spread, but the molecular mechanisms have not been elucidated. The aim of the present study was to investigate the regulatory function of BMP-2 in the migration of COS-7 cells and the underlying mechanisms.  相似文献   

ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines     

Rui Bai  Zhong Shi  Jia-wei Zhang  Dan Li  Yong-liang Zhu  Shu Zheng 《Journal of Zhejiang University. Science. B》2012,13(11):884-893

  相似文献   

运动对自然杀伤细胞免疫的影响   总被引：1，自引：0，他引：1  

王建密  邢良美  李洁 《辽宁体育科技》2007,29(2):11-12

自然杀伤细胞(NK)以其独特的细胞毒活性在机体对抗各种病毒的第一道防线中起着重要作用,其数量和活性是反映机体免疫系统功能的重要指标。中小强度有规律的运动可以提高NK细胞数量及NK细胞的毒活性,增强机体的免疫功能;而大强度力竭运动则降低NK细胞的数量和毒活性,减弱机体的免疫功能。  相似文献   

Correlation of matrix metalloproteinase-2, −9, tissue inhibitor-1 of matrix metalloproteinase and CD44 variant 6 in head and neck cancer metastasis     

徐娅苹  赵学群 《浙江大学学报(A卷英文版)》2003,4(4):491-501

This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase-2, −9 (MMP-2, MMP-9), tissue inhibitor-1 of matrix metalloproteinase (TIMP-1), cell adhesion molecule 44 variant 6 (CD44v6), HER2/neu and p53 was investigated in 154 patients with head and neck squamous cell carcinoma (SCC) by ABC and ImmunoMax immunohistochemical method. Their clinical relevance and correlation were analysed. The expression of MMP-2, MMP-9, TIMP-1, CD44v6, HER2/neu and p53 was found in cancer cells in 87.01%, 85.71%, 68.18%, 98.05%, 55.19% and 50.65% cases respectively. Linear regression and correlation analysis revealed that there was close positive relationship (P<0.05) between the expression of MMP-2 and MMP-9, TIMP-1 and CD44v6, HER2/neu and MMP-9, MMP-2 and p53. Up-regulation of MMP-2 was accompanied by advanced T stage (P<0.01). There was also a trend of MMP-2 expression being related with tumor metastasis. Increased expression of HER2/neu was found in patients with tumor recurrence (P<0.05). The expression of TIMP-1 was higher in laryngeal cancer than that in pharyngeal cancer, and higher in keratinizing and non-keratinizing SCC than that in basaloid SCC (P<0.05). These findings suggested that MMP-2 and MMP-9, HER2/neu and MMP-9, MMP-2 and p53 had a coordinate function in aggression of tumor; that MMP-2 had a more important function than MMP-9 in tumor invasion and metastasis; and that HER2/neu might serve as a biomarker for poor prognosis in HNSCC. Project supported by Lübeck Medical University, Germany  相似文献   

细胞生物学双语教学面临的问题及解决方法     

黄升谋  林淦 《襄樊学院学报》2006,27(5):118-120

文章详细论述了细胞生物学双语教学的意义、面临的问题及解决的对策,实施双语教学的具体方法、思路,重点讲述了课堂的教学组织以及课余的准备工作,为开展细胞生物学双语教学提供了理论基础.  相似文献   

CDMA网络定位方式及其应用   总被引：2，自引：0，他引：2  

丁力 《重庆职业技术学院学报》2004,13(4):99-101

本文从无线定位技术的原理出发详细介绍了CDMA网络中的定位三种方式以及定位技术的简单应用  相似文献   

Review:Acute lung injury/acute respiratory distress syndrome (ALI/ARDS): the mechanism,present strategies and future perspectives of therapies     

LUH Shi-ping  CHIANG Chi-huei 《Journal of Zhejiang University. Science. B》2007,8(1)

INTRODUCTION OF ACUTE LUNG IN- JURY/ACUTE RESPIRATORY DISTRESS SYN- DROME (ALI/ARDS) Acute lung injury (ALI) and its more severe form, the acute respiratory distress syndrome (ARDS) are syndromes of acute respiratory failure, which was defined by radiological (bilateral lung field infiltrates) and physiological (the ratio of arterial oxygen pres- sure and the inspiratory oxygen concentration, Pa O 2 /Fi O2≤300 mmHg for ALI and ≤200 mmHg for ARDS) criteria in…  相似文献