Determination of Biological Variance and Validation of a Fluorometric Assay for Measurement of α-l-Iduronidase Activity in Dried Blood Spots Samples: The First Experience in Iran     

Mohammad Abdi  Mohammad Said Hakhamaneshi  Mohammad Reza Alaei  Namam-Ali Azadi  Rahim Vakili  Daniel Zamanfar  Mohammad Taghikhani  Shohreh Khatami 《Indian journal of clinical biochemistry : IJCB》2015,30(3):318-322

Methods for assaying lysosomal diseases in dried blood samples are very useful today due to its several advantages related to the stability of samples, its transportation, handled and analysis, and its potential use for newborn screening compared to traditional methods in leucocytes samples. For this reason, it is important to validate these assays before being used in routine laboratory. Because of different in biological markers based on ethnicity, we aimed this study to validation a DBS-based fluorometric assay for measurement of α-l-Iduronidase activity for diagnosis of MPS I patients in Iran. DBS samples were collected from 15 MPS I patients and 60 healthy age matched subjects. Diagnostic value, biological variance and α-l-Iduronidase activity were determined. DBS α-l-Iduronidase activity was significantly higher in male subjects than in female group. Using a cut-off level of 1.08 µmol/spot 20 h, sensitivity and specificity were 100 and 98 %. The linearity of test was proved and we showed that within-run and between run precision were 5.6 and 14.66 %. Measurement of α-l-Iduronidase activity in DBS samples is an accurate test for diagnosis of MPS I and because of its rapid shipping and simplicity to keeping, DBS-based enzyme activity could be considered as a useful diagnostic tool in this disease.  相似文献   

The effect of l-citrulline and watermelon juice supplementation on anaerobic and aerobic exercise performance     

Paul T. Cutrufello  Stephen J. Gadomski  Gerald S. Zavorsky 《Journal of sports sciences》2015,33(14):1459-1466

Citrulline has been proposed as an ergogenic aid, leading to an interest in watermelon given its high citrulline concentration. The aim of this study was to determine the effects of a single, pre-exercise dose of l-citrulline, watermelon juice, or a placebo on the total maximum number of repetitions completed over 5 sets, time to exhaustion, maximal oxygen consumption (VO₂max), anaerobic threshold, and flow-mediated vasodilation. A randomised double-blind within-participants study design was used to examine these effects among 22 participants (n = 11 males). Supplementation included either a 7.5% sucrose drink containing 6 g of l-citrulline, 710 mL of watermelon juice (~1.0 g citrulline), or a 7.5% sucrose placebo drink. Supplementation was administered 1 or 2 h before exercise testing to investigate a timing effect. There was no significant effect between the three supplements for the total number of repetitions, time to exhaustion, VO₂max, anaerobic threshold, or flow-mediated vasodilation. There was also no interaction observed relative to gender or supplement timing (P > 0.05). A single dose of l-citrulline or watermelon juice as a pre-exercise supplement appears to be ineffective in improving exercise performance; however, greater doses of l-citrulline have been shown to be safe and are currently left unexamined.  相似文献   

女子赛艇八单专项训练强度测试分析与运用     

孙朋  张国培 《湖北体育科技》2015,(6):516-518

通过监控女子赛艇八单赛前40日训练,对安静状态和不同桨频训练下血乳酸分析,寻找变化规律,找出不同桨频的适宜训练强度,为不同训练负荷提供理论依据。  相似文献   

阿魏酸镧配合物的合成与表征及热化学研究     

黄熠  唐伟军  蔡罗蓉  张芳  张伟 《湘南学院学报》2015,(2)

用阿魏酸与镧的氯化物合成了一种新的稀土配合物,采用红外光谱等对配合物进行了表征,确定了化学式为La(C10H9O4)3·2H2O,在常压、298.15K下,分别测定了阿魏酸、氢氧化钠、七水氯化镧、阿魏酸镧、氯化钠和水在混合溶剂(丙酮：无水乙醇：4mol·L-1HCl ＝1：1：1)中的溶解焓,并根据热化学原理得出了298.15 K下配合物的标准摩尔生成焓为(-2856.59±2.52)kJ·mol-1.  相似文献   

顺铂联合热处理对术中回收血红细胞及其中混杂的肝肿瘤细胞株(HepG2)的影响（英文）     

Jin-ting YANG  Li-hui TANG  Yun-qing LIU  Yin WANG  Lie-ju WANG  Feng-jiang ZHANG  Min YAN 《Journal of Zhejiang University. Science. B》2015,16(5):395-403

目的:研究离体热处理和顺铂联合热处理对术中回收血红细胞功能的影响及其中混杂的肝肿瘤细胞株(HepG2)的杀灭作用。创新点:(1)采用多种评价指标研究了不同时间离体热处理对术中回收血中混杂的HepG2的杀灭作用及对红细胞的影响,并确定了对红细胞安全且能有效杀灭HepG2的离体热处理时间。(2)从多个角度评价了离体顺铂联合热处理对术中回收血红细胞的影响及对其中混杂的HepG2的杀灭作用,确定了该方案中对红细胞安全且能有效去除HepG2的顺铂浓度。方法:采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)、5-乙炔基-2’脱氧尿嘧啶核苷(EdU)和平板克隆形成评估HepG2的细胞存活率、DNA复制率和克隆形成能力(图1和3);从红细胞渗透脆性、携氧能力(2,3-二磷酸甘油酸(2,3-DPG)、半饱和氧分压(P50))、能量代谢(Na+-K+-ATPase、pH)、膜完整性(游离血红蛋白(Hb)、血清K+和Na+浓度、细胞膜磷脂酰丝氨酸外翻比例)等角度评估红细胞功能(图2和4;表3)。结论:肝肿瘤术中回收血经离体顺铂联合热处理(42°C,50μg/ml)60min后,能有效清除其中混杂的HepG2,但对红细胞无显著影响,值得体内进一步研究顺铂热处理有效应用于肿瘤手术自体血液回输的安全方案。  相似文献   

Chlorogenic acid prevents acetaminophen-induced liver injury: the involvement of CYP450 metabolic enzymes and some antioxidant signals     

Chun Pang  Yu-chen Sheng  Ping Jiang  Hai Wei  Li-li Ji 《Journal of Zhejiang University. Science. B》2015,16(7):602-610

Chlorogenic acid (CGA), a polyphenolic compound, is abundant in fruits, dietary vegetables, and some medicinal herbs. This study investigated the prevention of CGA against acetaminophen (AP)-induced hepatotoxicity and its engaged mechanisms. CGA reversed the decreased cell viability induced by AP in L-02 cells in vitro. In addition, CGA reduced the AP-induced increased serum levels of alanine/aspartate aminotransferase (ALT/AST) in vivo. The effect of CGA on cytochrome P450 (CYP) enzymatic (CYP2E1, CYP1A2, and CYP3A4) activities showed that CGA caused very little inhibition on CYP2E1 and CYP1A2 enzymatic activities, but not CYP3A4. The measurement of liver malondialdehyde (MDA), reactive oxygen species (ROS), and glutathione (GSH) levels showed that CGA prevented AP-induced liver oxidative stress injury. Further, CGA increased the AP-induced decreased mRNA expression of peroxiredoxin (Prx) 1, 2, 3, 5, 6, epoxide hydrolase (Ephx) 2, and polymerase (RNA) II (DNA directed) polypeptide K (Polr2k), and nuclear factor erythroid-2-related factor 2 (Nrf2). In summary, CGA ameliorates the AP-induced liver injury probably by slightly inhibiting CYP2E1 and CYP1A2 enzymatic properties. In addition, cellular important antioxidant signals such as Prx1, 2, 3, 5, 6, Ephx2, Polr2k, and Nrf2 also contributed to the protection of CGA against AP-induced oxidative stress injury.  相似文献   

HPLC法同时测定热淋清胶囊中没食子酸、原儿茶酸、槲皮苷和槲皮素的含量     

杨芳芳  余伯阳 《凯里学院学报》2011,29(3):84-86

建立热淋清胶囊中没食子酸、原儿茶酸、槲皮苷和槲皮素的HPLC定量方法.采用Wa-ters色谱柱,乙腈-0.4%磷酸为流动相梯度洗脱,流速1.0 mL/min,检测波长254 nm,柱温40℃.没食子酸、原儿茶酸、槲皮苷和槲皮素的线性范围分别为1.376~9.632μg,0.041 6~0.291 2μg,0.061 5~0.430 5μg,0.006 702~0.046 914μg;平均回收率分别为98.71%(RSD=1.17%)、98.77%(RSD=1.92%)、99.19%(RSD=1.92%)、100.39%(RSD=1.99%).所建立的方法准确、稳定,重现性好,可用于热淋清胶囊中有效成分的定量分析.  相似文献   

HPLC 法测定舒泌通胶囊中绿原酸和蒙花苷的含量     

白文莉  郭文婷  马跃新  杨汝琪  庞仕龙 《昆明师范高等专科学校学报》2015,(3)

建立了 HPLC 法同时测定舒泌通胶囊中绿原酸和蒙花苷的质量分数,采用 SunfireTM-C18柱（4．6 mm ×250 mm,5μm）,柱温为30℃,流动相 A 为甲醇、B 为0．5％冰醋酸,梯度洗脱,流速为1．0 mL／min,检测波长为328 nm．结果表明,二者的线性范围分别为1．0804～216．0804μg／mL 和1．0804～81．7200μg／mL．平均回收率分别为101．0％和9．3％,RSD 分别为3．4％和3．3％（n ＝9）．该方法准确、可靠,具有良好的重复性和稳定性,可用于舒泌通胶囊中绿原酸和蒙苷花的质量分数测定．  相似文献   

武昌鱼脂肪酸的气相色谱-质谱分析     

刁全平  侯冬岩  郭华  洪学莲  回瑞华 《鞍山师范学院学报》2015,(2):45-47

为了研究武昌鱼中脂肪酸的组成及含量,采用Bligh-Dyer提取法对样品中脂肪油进行提取,甲酯化后利用气相色谱-质谱联用仪进行分析测定．结果发现从武昌鱼中鉴定出14种脂肪酸,其中饱和脂肪酸5种,相对百分含量占总检出量的28．46％;不饱和脂肪酸9种,相对百分含量占总检出量的71．27％．  相似文献   

SYSMEX UF-1000i尿有形成分分析仪对尿路感染的诊断价值     

战伟 《大连大学学报》2015,(3):100-102

采用Sysmex UF-1000i尿有形成分分析仪和AX-4280尿干化学分析仪测定尿标本,同时进行尿细菌定量培养。把测定值与尿培养金标准比对,干化学法白细胞酯酶和UF-1000i细菌计数结果的一致性适中,与亚硝酸盐结果的一致性较低,与尿白细胞计数结果的一致性极高。细菌定量培养的特异性、灵敏度及一致性高于UF-1000i白细胞计数。UF-1000i在临床快速诊断尿路感染时应用价值较高。  相似文献