人类肥胖基因克隆和表达     

吴武  徐明 《成都体育学院学报》2003,29(5):84-87

以编码人全长的OBmRNA为模板,用RT-PCR法获得了编码146个氨基酸残基的OBcDNA,并经EcoRI和BamHI双酶切克隆至原核质表达质粒pGEX-1λT中,通过IPTG诱导,以谷胱苷肽巯基转移酶融合蛋白(GSP)方式进行了表达。结论:在原核细胞中获得高效表达。  相似文献   

Advances in the phylogenesis of Agaricales and its higher ranks and strategies for establishing phylogenetic hypotheses     

Zhao RL  Desjardin DE  Soytong K  Hyde KD 《Journal of Zhejiang University. Science. B》2008,9(10):779-786

We present an overview of previous research results on the molecular phylogenetic analyses in Agaricales and its higher ranks (Agaricomycetes/Agaricomycotina/Basidiomycota) along with the most recent treatments of taxonomic systems in these taxa. Establishing phylogenetic hypotheses using DNA sequences, from which an understanding of the natural evolutionary relationships amongst clades may be derived, requires a robust dataset. It has been recognized that single-gene phylogenies may not truly represent organismal phylogenies, but the concordant phylogenetic genealogies from multiple-gene datasets can resolve this problem. The genes commonly used in mushroom phylogenetic research are summarized.  相似文献   

生物分子系统学研究中的RAPD技术   总被引：1，自引：0，他引：1  

邱小忠  刘莹 《赣南师范学院学报》1996,(6):64-67

RAPD技术在Williams等人于1990年首先提出后,在检测DNA多态性方面受到了广泛的应用.RAPD技术以其简单、快速、信息量大等特点渗透在基因诊断,法医检测,生物药品的检验,生物种群关系的研究各个领域.以核酸为指标构建的生物系统进化树,不受主观因素的影响,避免了经典植物系统学的人为影响因素,因而受到广泛应用,在分子系统研究中出现了RFLP,RAPDDNA和RNA测序等分子生物学技术,其中RAPD技术不需要经过DNA分子克隆、分子杂交等繁锁步骤,也不需用放射性同位素显示,并且多态性检出率高,因而受到普遍关注.  相似文献   

丁酸异戊酯合成中的催化剂比较研究     

林松竹 《中国教育科研与探索》2006,(5):17-19

本文进行了硫酸、对甲苯磺酸、氨基磺酸、强酸性阳离子交换树脂、六水三氯化铁、二水二氯化锡、十八水硫酸铝、十二水合硫酸铁铵、一水硫酸氢钠、固体超强酸、杂多酸和沸石分子筛等催化剂催化合成丁酸异戊酯的比较研究。  相似文献   

Microwave-assisted synthesis, anticonvulsant activity and quantum mechanical modelling of N-（4-bromo-3-methylphenyl） semicarbazones     

Shalini M  Yogeeswari P  Sriram D  Induja S 《Journal of Zhejiang University. Science. B》2007,8(1):45-55

Objective： To study the effect of halo substitution on disubstituted aryl semicarbazones on the anticonvulsant potential and model the activity based on quantum mechanics. Methods： A series of twenty-six compounds of N^4-（4-bromo-3-methylphenyl） semicarbazones were synthesized and evaluated for the anticonvulsant activity in the maximal electroshock seizure （MES） and subcutaneous pentylenetetrazole （scPTZ） seizure threshold tests. Some potential compounds were also tested in the subcutaneous strychnine （scSTY） and subcutaneous picrotoxin （scPIC） seizure threshold tests. The synthesized compounds were tested for behavioral impairment and CNS （central nervous system） depression in mice. Quantum mechanical modelling was carried out on these compounds to gain understanding on the structural features essential for activity. Results： Some compounds possessed broad spectrum anticonvulsant activity as indicated by their effect in pentylenetetrazole, strychnine, picrotoxin and maximal electroshock seizures models in resemblance to other aryl semicarbazone derivatives reported earlier. The higher the difference in HOMO （highest occupied molecular orbital） and LUMO （lowest unoccupied molecular orbital） energy levels was, the greater was the activity profile. Conclusion： The pharmacophoric requirements for compounds to exhibit anticonvulsant activity that includes one aryl unit in proximity to a hydrogen donor-acceptor domain and an electron donor have been justified with the molecular orbital surface analysis of the synthesized compounds.  相似文献   

VDR,RXR, Coronin-1 and Interferonγ Levels in PBMCs of Type-2 Diabetes Patients: Molecular Link between Diabetes and Tuberculosis     

Kirtimaan Syal  Anand Srinivasan  Dibyajyoti Banerjee 《Indian journal of clinical biochemistry : IJCB》2015,30(3):323-328

Diabetes and tuberculosis are world’s most deadly epidemics. People suffering from diabetes are susceptible to tuberculosis. Molecular link between the two is largely unknown. It is known that Vitamin A receptor (RXR) heterodimerizes with Vitamin D receptor (VDR) and Peroxisome proliferator-activator receptor-γ (PPARγ) to regulate Tryptophan-aspartate containing coat protein (TACO) expression and fatty acid metabolism respectively, so it would be interesting to check the expression of these genes in diabetes mellitus (DM) patients which might explain the susceptibility of diabetics to tuberculosis. In this study, we checked the expression of RXR, VDR, TACO and Interferon-γ (IFNγ) genes in type-2 DM patients for understanding the link between the two diseases. We observed down regulation of RXR gene and corresponding up regulation of TACO gene expression. We have not observed significant change in expression of VDR and IFNγ genes in type-2 DM patients. Repression of RXR gene could hamper VDR-RXR heterodimer formation and thus would up regulate TACO gene expression which may predispose the type-2 DM patients to tuberculosis. Also, decrease in RXR-PPARγ heterodimer could be involved in DM.  相似文献   

《分子生物学》教学改革与实践新探     

姜立春  游章强  李俊刚  陈希文  闫秋洁 《绵阳师范学院学报》2015,(5)

《分子生物学》是一门理论性和实践性较强的学科.通过实施优化教师队伍、教学内容、教学模式、理论与实践相结合和课程考核方式等一系列创新性教学改革手段,以达到强化大学生的理论知识和实验动手技能、提高其分析和解决问题的能力、培养创新性应用型人才的目的 .  相似文献   

金丝桃素和β-环糊精结合常数的测定     

张晖  薛洪宝  李柏青  梁丽丽  陶兆林 《河西学院学报》2015,(2):25-30

采用微量热泳动法和分子荧光光谱法分别测定金丝桃素与β-环糊精的包合物和结合常数,并对实验数据进行Hill拟合.实验表明:β-环糊精对金丝桃素有一定程度的包合.两种方法测得的金丝桃素和β-环糊精包合物中金丝桃素、β-环糊精的分子个数比分别为1:5和1:7,微观解离常数KA分别为1.82ng·L-1和3.72ng·L-1,平衡解离常数Kd分别为76.92和1120.23.该方法快速、简便、试验结果相对可靠.  相似文献   

A recessive gene controlling male sterility sensitive to short daylength/low temperature in wheat (Triticum aestivum L.)     

Chen XD  Sun DF  Rong DF  Peng JH  Li CD 《Journal of Zhejiang University. Science. B》2011,12(11):943-950

Utilization of a two-line breeding system via photoperiod-thermo sensitive male sterility has a great potential for hybrid production in wheat (Triticum aestivum L.). 337S is a novel wheat male sterile line sensitive to both short daylength/low temperature and long daylength/high temperature. Five F₂ populations derived from the crosses between 337S and five common wheat varieties were developed for genetic analysis. All F₁’s were highly fertile while segregation occurred in the F₂ populations with a ratio of 3 fertile:1 sterile under short daylength/low temperature. It is shown that male sterility in 337S was controlled by a single recessive gene, temporarily designated as wptms3. Bulked segregant analysis (BSA) coupled with simple sequence repeat (SSR) markers was applied to map the sterile gene using one mapping population. The wptms3 gene was mapped to chromosome arm 1BS and flanked by Xgwm413 and Xgwm182 at a genetic distance of 3.2 and 23.5 cM, respectively. The accuracy and efficiency of marker-assisted selection were evaluated and proved essential for identifying homozygous recessive male sterile genotypes of the wptms3 gene in F₂ generation.  相似文献   

基于创新能力培养的实验教学改革的探索——以“生物化学与分子生物学”为例     

王怀颖  靳祎 《教育教学论坛》2022,(2)

实验教学是培养大学生创新能力的重要途径和手段,但是传统实验教学模式存在较多问题,不能满足培养高素质医学人才的要求。"生物化学与分子生物学"是临床医学专业的基础课,而实验教学是该课程教学内容的一部分,是培养医学生创新能力的重要途径和手段。以"生物化学与分子生物学"实验教学为例,从优化实验教学设计、丰富实验教学内容、改革考核方式等方面进行实践和总结,阐述如何在实验教学中培养学生创新思维,提升实验教学质量,为实验教学改革提供新的思路和参考。  相似文献