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本研究以金线莲茎尖作为外植体,研究了不同激素浓度对金线莲茎尖诱导、增殖及生根率的影响。结果表明：对于金线莲比较适合的诱导培养基是MS＋6-BA4-．0mg／L．＋ZT0．2mg／L＋NAA0．5mg／L;增殖较佳的组合是1／2Ms＋6-BA0-4mg·L-1＋NAA0·2mg·L-1。当NAA的浓度达到2·0mg·L-1时生根率最高,可以达到100％。 相似文献
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以碗莲的茎尖为实验试材,研究基本培养基加入不同种类、浓度的生长调节剂,对离体碗莲茎尖萌发的影响。结果表明：诱导茎尖萌发较理想的培养基为MS＋6-BA1.0mg/L＋NAA0.25mg/L＋GA31.0mg/L＋3%蔗糖;适宜的增殖培养基为：MS＋6-BA2.0mg/L＋NAA0.25mg/L＋3%蔗糖;生根培养基为：MS＋IBA1.0mg/L。光照14h/d.总结了碗莲茎尖组织培养上存在的问题,并对解决某些问题提出了一些建议。 相似文献
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本研究以红叶石楠茎尖作为外植体,研究了不同激素浓度对红叶石楠茎尖诱导、增殖及生根率的影响。结果表明：对于红叶石楠增殖最佳的组合是Ms＋6-BA0.4mg.L-1＋NAA0.2mg.L-1。当NAA的浓度达到1.0 mg.L-1时生根率最高,可以达到100%。 相似文献
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采用3种基本培养基、不同浓度的NAA和6-BA组合对蝴蝶兰瓶苗诱导生根的试验研究,结果表明:培养基商兰1号为优良的生根基本培养基,显著优于常规MS培养基;6-BA0.lmg/L NAA0.5mg/L为优良的激素浓度组合,植株生根良好。 相似文献
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BackgroundIn order to produce an effective callus in Echinacea purpurea L.; determination of the explant type and growth regulators that best respond to callus induction and the optimization of the culture conditions to increase the amount of caffeic acid derivatives (CADs) in the obtained callus. CADs contents of callus cultures of E. purpurea were evaluated by establishing an effective callus induction system in vitro.ResultsVarious medium containing different growth regulators were tested using leaf, petiole, cotyledon and root as the explants. The best callus development was achieved in MS medium with 1.0 mg l−1 2,4-D + 2.0 mg l−1 BAP in leaf, 1.0 mg l−1 NAA + 0.5 mg l−1 TDZ in petiole, 2.0 mg l−1 NAA + 1.0 mg l−1 TDZ in cotyledon and 0.5 mg l−1 NAA + 0.5 mg l−1 BAP in roots. Upon optimisation of callus growth, each type of explant was cultured for 4, 6, 8 and 10 weeks in medium for the analyses of caftaric acid, chlorogenic acid, caffeic acid and chicoric acid contents. The highest amounts of caftaric acid (4.11 mg/g) and chicoric acid (57.89 mg/g) were found from petiole explants and chlorogenic acid (8.83 mg/g) from root explants at the end of the 10-week culture time.ConclusionsAs a result of the present study, the production of caffeic acid derivatives was performed by providing the optimization of E. purpurea L. callus cultures. Effective and repeatable protocols established in this study may offer help for further studies investigating the production of caffeic acid derivatives in vitro.How to cite: Tanur Erkoyuncu M, Yorgancilar M. Optimization of callus cultures at Echinacea purpurea L. for the amount of caffeic acid derivatives. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.02.003. 相似文献
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采用水培试验的方法,研究不同浓度铜（0、20、40、60、80和100mg/L）胁迫条件下小叶黄杨的生长和生理特征。结果表明,随铜浓度的增加,小叶黄杨的生物量（鲜重）逐渐下降;蛋白质含量呈下降趋势;叶绿素含量先上升后下降;丙二醛的含量逐渐增加;超氧化物歧化酶和过氧化物酶的活性均呈先增加后下降的趋势。 相似文献
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BackgroundThis research is intended to determine suitable types and concentrations of plant growth regulators (PGRs) to induce callus on stem and leaf sections of 4 species of the genus Garcinia, namely, Garcinia mangostana, Garcinia schomburgkiana, Garcinia cowa, and Garcinia celebica. The base medium was MS medium containing 30 g l-1 sucrose, 0.5 g l-1 polyvinylpyrrolidone (PVP), and 7 g l-1 agar, and for the different treatments, PGRs were added to the medium as follows: thidiazuron (TDZ) at concentrations of 0, 0.1, 0.5, 1, and 2 mg l-1; 6-(3-hydroxybenzylamino) purine (meta-topolin) at concentrations of 0, 0.5, 2.5, and 5 mg l-1; 4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid (picloram) at concentrations of 0, 0.5, 2.5, and 5 mg l-1; and 2,4-dichlorophenoxyacetic acid (2,4-D) at concentrations of 0, 0.5, 1, 2, and 4 mg l-1. The occurrence of callus was observed after 4 weeks.ResultsA maximum of 100% and 93% of G. mangostana leaf explants formed callus in the 0.5 mg l-1 and 1 mg l-1 TDZ treatments, respectively, while 100% of G. schomburgkiana stem explants formed callus in the 1 mg l-1 TDZ treatment and 89% of G. schomburgkiana leaf explants formed callus in the 0.5 mg l-1 picloram treatment. The highest callus induction rate for G. cowa was 62% in the 1 mg l-1 TDZ treatment and for G. celebica was 56% in the 0.5 mg l-1·mT-1 treatment.ConclusionsFor all 4 species, the greatest amount of large nodular callus was observed in the TDZ treatments. White, friable callus was observed on most of the 2,4-D and picloram treatment groups. Most meta-topolin treatments resulted in minimal callus formation.How to cite: Suwanseree V, Phansiri S, Yapwattanaphun C. A comparison of callus induction in 4 Garcinia species. Electron J Biotechnol 2019;40. https://doi.org/10.1016/j.ejbt.2019.04.006 相似文献
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采用盆栽法考察不同质量浓度的Cd、Zn、Cu、Pb、As 5种重金属复合胁迫对酢酱草抗氧化酶活性的影响。结果显示随着重金属处理浓度梯度的增加,酢酱草过氧化物酶(POD)活性、抗坏血酸过氧化物酶(APX)活性、脯氨酸(PRO)含量及谷胱甘肽(GSH)含量先升高后降低;而超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)酶活性逐渐下降。结果表明酢酱草在重金属复合胁迫下能防御性的增加POD酶活性、SOD酶活性、PRO含量及GSH含量以减少逆环境的伤害,而重金属复合胁迫浓度过高则会导致酢酱草抗氧化酶系统受到明显损害,生长受到很大程度的抑制。 相似文献
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在0.01mg/L NAA诱导下，离体黄瓜下胚轴切段显示了较强的发根能力，发根率达88％以上，切片观察显示，在NAA诱导24h时，下胚轴切段维管柱中靠近外韧皮部外则的薄壁细胞发生变化，细胞质变浓，细胞核变大，部分细胞分裂并形成分生组织结节；48h时，分裂的细胞团增大，逐渐形成根原基，96h时，不定根中分化出许多网纹导管分子并与下胚轴切段的导管分子相连，而且大量不定根穿破表皮达到肉眼可见的程度，下胚轴切段正插时，不定根在形态学下端发生；反插时，不定根在形态学上，下两端发生，切片观察显示，正插和反插时不定根发生部位相同，均位于外韧皮部外侧的薄壁细胞处，但插时不定根发生在时间上比正插要快1天左右。 相似文献
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目的:在大田常规管理条件下,宁粳1号和两优培九两种水稻根系在整个生育期的铅垂向.水平向二维时空分布.方法:在盆栽条件下,应用环刀定点连续取样法,对这两种水稻整个生长期的根系分布进行了研究.结果:两优培九的根系干重和体积在各个生育期都均高于宁粳1号,但在灌浆乳熟后期衰亡速度也大于宁粳1号;两种水稻根系干重和体积总量呈现先增加后降低的趋势,从分蘖期到抽穗开花期不断增大,在抽穗开花后期达到最大,随后逐渐死亡衰减.结论:两种水稻根系干重密度和体积密度在水平向上呈距离植株越远越小的趋势,采用幂函数拟合效果最好,并且80%以上的根系集中在距离植株10cm范围内;铅垂向随土层深度的增加而减小的趋势,同样也是幂函数拟合效果最好,85%以上的根系集中在土层深度25cm范围内;根系干重和体积与地上总干物质重,叶面积具有显著相关性. 相似文献
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Kalpana Luthra Suman Vasisht Shivani Chhabra K. R. Raju D. P. Agarwal S. C. Manchanda L. M. Srivastava 《Indian journal of clinical biochemistry : IJCB》1998,13(1):12-19
Lipoprotein Lp(a) excess has been identified as a powerful predictor of premature atherosclerotic vascular diseases. To evaluate
this in a North-Indian population, 130 CAD patients and 130 controls were analyzed. The size of the apo(a) phenotypic isoforms
was inversely proportional to Lp(a) concentrations. The mean concentration of Lp(a) in the CAD patients was 42±34 mg/dl whereas
in the normal subjects it was much lower, 27±27 mg/dl. 157 subjects out of the total 260 subjects showed plasma levels of
>20mg/dl. The frequency of high Lp(a) levels was much higher in patients(73%) than controls (43%). These data suggest (1)
that there is heterogeneity of the Lp(a) polymorphism, (2) Higher Lp(a) levels were found in patients than in the controls,
(3) Patients showed 1.5 fold increase in Lp(a) levels as compared to the controls. We conclude that low molecular weight apo(a)
isoforms are significantly associated with increased risk of CAD in the North-Indian population. 相似文献
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4mg and 8mg monosodium glutamate per gram body weight was administered subcutaneously for 6 consecutive days to normal adult
male mice and its effect was seen on 31st day after the last injection on some antioxidant enzymes in heart. A significant dose dependent increase in lipid peroxidation
and xanthine oxidase level was observed, whereas the activity of free radical scavenging enzymes such as superoxide dismutase
and catalase was decreased in both monosodium glutamate treated groups (Group-2 and Group-3). So, the present work suggested
that monosodium glutamate at dose level of 4mg/g body weight and above induced oxidative stress in the cardiac tissue by changing
the activity of free radical initiating enzyme such as xanthine oxidase and scavenging enzymes like superoxide dismutase and
catalase. 相似文献