共查询到20条相似文献，搜索用时 125 毫秒
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仙人掌科植物仙人山，其茎段切面通过在MS+6-BA0.5mg/l+2.4-D2mg/l+NAA0．1mg/l琼脂培养基中的组织培养，诱导出愈伤组织．本文将对其予以介绍． 相似文献
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这篇章着重比较分析了榛子的芽和幼茎愈伤组织中紫杉醇的合成能力。用高效液相色谱仪测定榛子，结果显示芽比幼茎含量更高。用2．0mg/L BA和1．4mg/C LNAA诱导幼芽，8天后的愈伤组织以100％的速度感应。较好的培养愈伤组织的方法是用0．5mg/L2，4-D、1．0mg/L NAA和0．5mg/L KT补充B5培养基。测得愈伤组织的生长速度和紫杉醇的含量分别是7．74g／L和0．13％。 相似文献
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用WPM为基本培养基 ,分别附加不同的植物生长调节剂。四种处理为 0 2 2mg/l 2 ,4—D 5mg/l 2iP ;0 .2 2mg/l 2 ,4—D 0 .5mg/l TDZ ;0 .1 8mg/l NAA 0 .5mg/l TDZ ;0 .5mg/l TDZ。使用外植体为杜鹃 (Rhododendronsimsii)一栽品种′HellmutVogel′的组培苗叶愈伤组织。试验结果是在处理NAA TDZ和TDZ上诱导出不定芽。所用外植体为来自杜鹃 (Rhododendronsimsii) 5个栽培品种 ,即′Paloma′ ,′Mme .A .Haerens′,′Lara′,′PetrickAlba′和′FrauUrsulaHerrman′和组培苗叶愈伤组织。结果是除栽培品种′Lara′外 ,其余四个栽培品种都诱导出不定芽 ,其中两个栽培品种仅在处理NAA TDZ上形成不定芽。总体上看 ,无论是在维持愈伤组织生长方面 ,还是在诱导不定芽形成方面 ,处理NAA TDZ都优于处理TDZ。 相似文献
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樱桃番茄的组织培养与快速繁殖研究 总被引：1，自引：0，他引：1
以樱桃番茄新品种“圣女”和“金珠”的叶片、茎段和幼芽为外植体进行试管培养 ,结果表明 :叶片、茎段和幼芽均可诱导形成愈伤组织 ,愈伤组织可分化出芽丛 ,腋芽可直接萌生 ,不定芽可诱导生根成完整植株 .经试验筛选出各培养阶段的最适培养基为 :(1)愈伤组织诱导 :叶片以MS Kt4mgL IAA8mgL为最佳 ;茎段和幼芽以MS NAA0 1mgL 6 -BA1 5mgL为最佳 ;(2 )愈伤组织芽的分化 :MS IAA0 2mgL 6 -BA2mgL ;(3)腋芽萌生 :MS 6 -BA2 5mgL IBA0 1mgL ;(4)生根 :无激素的Ms或Mso . 相似文献
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范玉清 《晋东南师范专科学校学报》2000,17(3):8-10
用WPM为基本培养基，分别附加不同的植物生长调节剂。四种处理为0．22mg/l 2,4-D 5mg/l 2ip;0.22mg/l 2,4-D 0.5mg/l TDZ;0.18mg/l NAA 0.5mg/l TDZ;0.5mg/l TDZ.使用外植体为杜鹃（Rhododendron simsii)一栽品种‘Hellmut Vogel’的组培苗叶愈伤组织。试验结果是在处理NAA＋TDZ和TDZ上诱导出不定芽。所用外植体为来自杜鹃（Rhododendron simsii)5个栽培品种，即‘Paloma’,‘Mme.A.Haerens’,‘Lara’,‘Petrick Alba’和‘Frau Ursula Herrman’和组培苗叶愈伤组织。结果是除栽培品种‘Lara’外，其余四个栽培品种都诱导出不定芽，其中两个栽培品种仅在处理NAA＋TDZ上形成不定芽。总体上看，无论是在维持愈伤组织生长方面，还是在诱导不定芽形成方面，处理NAA＋TDZ都优于处理TDZ。 相似文献
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蔡汉权 《韩山师范学院学报》2005,26(6):72-75
以菊花花瓣为外植体，在无菌条件下诱导愈伤组织，再由愈伤组织进行芽诱导．利用花瓣组织培养易变异的特性，以期培养出新品种．本实验中探讨了6-BA、NAA在诱导愈伤组织和芽诱导中的影响，并获得MS＋6-BA2mg／L＋NAA0．5mg／L为芽诱导的最佳培养方秦． 相似文献
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以密叶罗勒的茎尖为外植体,以期筛选出诱导愈伤组织分化和丛生芽生根的最佳培养基。同时研究了密叶罗勒组织培养中材料的消毒时间对消毒效果的影响。试验结果表明,培养基MS＋6-BA5．0mg/L＋NAA0．1mg/L是愈伤组织诱导分化相对较好的培养基;培养基MS＋6-BA0．3mg/L＋NAA0．6mg／L是罗勒生根培养较好的培养基。在以75％的酒精消毒30秒后,用0．1％的升汞消毒2min,对试验材料的消毒效果相对较好。 相似文献
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王廷晋 《思茅师范高等专科学校学报》2008,24(6)
以滇润楠（Machilus yunnanensis）的侧芽、顶芽为材料。在MS培养基附加细胞分裂素BA、KT和生长素NAA、2,4-D中进行培养,诱导愈伤组织以及侧芽、顶芽,结果表明,MS＋2,4-D0．5mg／l＋BA2mg／1＋AC0．3％诱导滇润楠根部萌蘖条愈伤组织的产生效果较好,MS＋2,4-D0．1mg／I＋KT2mg／1＋AC0．3％诱导滇润楠的侧芽萌动效果较好,MS＋NAA1mg／l＋BA1．5mg／l＋AC0．3％诱导滇润楠的硕芽萌动效果较好。 相似文献
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芦荟叶片愈伤组织诱导的研究 总被引：1，自引：0，他引：1
以中华芦荟、库拉索芦荟和木立芦荟的叶片为外植体进行组织培养。结果表明：三种芦荟均可以在特定的培养基中形成愈伤组织。中华芦荟愈伤组织的诱导最佳培养基为：MS 6-BA0.2mg／L 2，4-D0．5mg／L；库拉索芦荟愈伤组织的诱导最佳培养基为：MS 6-BA1.0mg／L 2，4-D1．0mg／L；木立芦荟愈伤组织的诱导最佳培养基为：MS 6-BA2．0mg／L 2，4-D1．5mg／L。 相似文献
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朱缨花愈伤组织的诱导 总被引：1，自引：0，他引：1
研究了不同外植体和不同生长调节剂对朱缨花愈伤组织诱导的影响．结果表明：不同外植体的愈伤组织诱导能力为茎段〉带节茎段〉叶柄〉叶片，茎段和带节茎段是愈伤组织诱导的最佳外植体；诱导朱缨花茎段愈伤组织的最佳生长调节剂组合为MS＋NAA1mg／L和MS＋BA0．5mg／L＋NAA1mg／L;诱导朱缨花带节茎段愈伤组织的最佳生长调节剂组合为MS＋BA2mg／L＋NAA1．5mg／L． 相似文献
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Embryogenic calli were induced from the seeds of creeping bentgrass (Agrostis palustris Huds.) cv. Regent and colonial bentgrass (Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4-7 days and then co-cultivated with Agrobacterium tumefaciens, LBA4404, which contains plasmid vector-pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding region and matrix attachment region (MAR). After 3 days of co-cultivation, the calli were washed thoroughly and transferred to MS medium containing 2 mg/L of 2, 4-D, 12-15 mg/L phosphinothricin (PPT) and 250 mg/L of cefotaxime. After 2-3 months of selection, the actively growing calli of 'Regent' and 'Tiger' were transferred to MS medium with 12-15 mg/L PPT and 250 mg/L cefotaxime for regeneration. The putative transformants were maintained on MS medium with 3 mg/L PPT for long period but control died within 1 month. After establishing in greenhouse, the transformants also showed strong resistance to 0.4% of herbicide Basta but control plants died within 2 weeks. Under confocal microscope, both young leaves and roots showed significant GFP expression. PCR analysis revealed the presence of a DNA fragment of GFP gene at the expected size (380 bp) in the transformants and its absence in a randomly selected control plant. 相似文献
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柴明良 汪炳良 KIM Jae-yeoul LEE Jong-min KIM Doo-hwan 《Journal of Zhejiang University. Science. B》2003,(3)
INTRODUCTIONCreepingbentgrass (AgrostispalustrisHuds.)isanoutstandingcool seasonspeciesavailableforuseongolfcourseputtinggreens,tees,andcloselymowedfairways.Althoughco lonialbentgrass (AgrostistenuisSibth .Fl.Ox en .)isnotwelladaptedtotheverylowmowingheig… 相似文献
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Embryogenic calli were induced from the seeds of creeping bentgrass (Agrostis palustris Huds.) cv. Regent and colonial bentgrass (Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4–7 days and then co-cultivated withAgrobacterium tumefaciens, LBA4404. which contains plasmid vector-pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding
region and matrix attachment region (MAR). After 3 days of co-cultivation, the calli were washed thoroughly and transferred
to MS medium containing 2 mg/L of 2, 4-D, 12–15 mg/L phosphinothricin (PPT) and 250 mg/L of cefotaxime. After 2–3 months of
selection, the actively growing calli of ‘Regent’ and ‘Tiger’ were transferred to MS medium with 12–15 mg/L PPT and 250 mg/L
cefotazime for regeneration. The putative transformants were maintained on MS medium with 3 mg/L PPT for long period but control
died within 1 month. After establishing in greenhouse, the transformants also showed strong resistance to 0.4% of herbicide
Basta but control plants died within 2 weeks. Under confocal microscope, both young leaves and roots showed significant GFP
expression. PCR analysis revealed the presence of a DNA fragment of GFP gene at the expected size (380 bp) in the transformants
and its absence in a randomly selected control plant. 相似文献
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用MS+2,4-D1.0 mg/L+6%蔗糖培养基诱导三种柚叶片愈伤组织,悬浮培养法扩大培养.进行有效成分分析.结果表明沙田柚含有较高黄酮物质、柚皮苷和野漆树苷,结溪蜜柚次之,江津柚含量最低,约为沙田柚的50-60%.柚苷元在三个品种愈伤组织中含量相似.该结果为柚类进一步遗传转化和改良提供了基础数据. 相似文献
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以蚕豆叶片为材料,借助药理学实验和共聚焦技术结合一氧化氮（N0）探针DAF-2DA证明光下乙烯合成前体1-氨基环丙烷-1-羧酸（ACc）和乙烯气体均能显著诱导气孔关闭,且均表现明显浓度依赖效应。另外,一氧化氮合酶（NOS）抑制荆NG-氮-L-精氨酸-甲酯能够逆转ACC和乙烯气体诱导气孔关闭的效应,并能阻断ACC和乙烯气体诱导的气孔保卫细胞内NO水平的提高。结果表明乙烯可能通过NO$途径诱导的蚕豆保卫细胞内NO水平的提高导致气孔关闭。 相似文献
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It has been shown that the anterior cingulate cortex (ACC) and its dopamine system are crucial for decision making that requires physical/emotional effort, but not for all forms of cost–benefit decision making. Previous studies had mostly employed behavioral tasks with two competing cost–reward options that were preset by the experimenters. However, few studies have been conducted using scenarios in which the subjects have full control over the energy/time expenditure required to obtain a proportional reward. Here, we assessed the roles of the ACC and its dopamine system in cost–benefit decision making by utilizing a “do more get more” (DMGM) task and a time–reward trade-off (TRTO) task, wherein the animals were able to self-determine how much effort or time to expend at a nosepoke operandum for a proportional reward. Our results showed that (1) ACC inactivation severely impaired DMGM task performance, with a reduction in the rate of correct responses and a decrease in the effort expended, but did not affect the TRTO task; and (2) blocking ACC D2 receptors had no impact on DMGM task performance in the baseline cost–benefit scenario, but it significantly reduced the attempts to invest increased effort for a large reward when the benefit–cost ratio was reduced by half. In contrast, blocking ACC D1 receptors had no effect on DMGM task performance. These findings suggest that the ACC is required for self-paced effort-based but not for time–reward trade-off decision making. Furthermore, ACC dopamine D2 but not D1 receptors are involved in DMGM decision making. 相似文献
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利用成龄蓝莓的茎段为外植体,培养在添加各种激素配比的改良WPM培养基上,研究蓝莓离体繁殖影响因素,筛选蓝莓离体最佳增殖和生根培养基及培养条件。结果表明:在WPM+ZT0.8mg/L的增殖培养基上,蓝莓的增殖数最大达45,在1/2WPM+IBA0.5mg/L的生根培养基上生根率在品种间存在差异,可达70-90%。通过炼苗,试管苗的成活率达85%以上,建立了蓝莓工厂化育苗技术体系,为蓝莓商品基地的建立提供了充足的优良苗木。 相似文献
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中药王不留行的组织培养技术初探 总被引：1，自引：0，他引：1
选取王不留行饱满种子培养无菌苗,用无菌苗的茎和叶作为外植体,分别接种MS基本培养基外加激素NAA和6-BA诱导愈伤组织和不定芽,使用1/2MS培养基,附加IBA或NAA进行不定芽的生根实验,最后炼苗和移栽.实验结果表明王不留行的最优组织培养方案:种子用0.1%升汞消毒8m in,无菌湿脱脂棉催芽,茎和叶作为外植体,使用MS 0.1mg/L NAA 0.1mg/L 6-BA诱导愈伤组织和不定芽,1/2MS 0.15mg/L NAA诱导植株生根. 相似文献