共查询到20条相似文献，搜索用时 46 毫秒
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文章研究旨在优化前期筛选到的米曲霉HML366产β-葡萄糖苷酶的培养基条件。通过单因素实验和响应面设计对米曲霉HML366产β-葡萄糖苷酶的培养基条件进行了优化组合,确定最佳的发酵培养基配方为:甘蔗渣3.49%,豆饼粉0.375%,KH2PO4 0.255%。在此培养条件下得到β-葡萄糖苷酶酶活力达92.64 U/g,是未优化前酶活71.12 U/g的1.30倍。 相似文献
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几丁质酶(Chitinase,EC3.2.1.14)作为一类糖基水解酶,可以水解几丁质的β-1,4-糖苷键,其降解产物广泛应用在抗虫、医药、食品等领域。随着几丁质酶理论基础和应用的研究日益受到重视,几丁质酶的研究已成为几丁质科学中的不可忽视的分支。本文综述了微生物几丁质酶的分类、结构、酶学性质及应用现状,以期为几丁质酶的进一步利用提供参考。 相似文献
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响应面法优化解酯假丝酵母Candida lipolytica产脂肪酶发酵条件 总被引：4，自引：0，他引：4
用响应面法对Candida lipolytica液体发酵产脂肪酶的发酵条件进行了快速优化。首先运用逐因子试验确定Candida lipolytica产脂肪酶的最佳碳源和氮源分别为葡萄糖和蛋白胨。在此基础上,通过Plackett-Burman设计对影响其产酶相关因素进行评估并筛选出具有显著效应的三个因素:蛋白胨,KH2PO4,初始pH。在用最陡爬坡试验逼近以上三个因子的最大响应区域后,采用响应面分析法,确定其最优发酵条件:葡萄糖0.250%,蛋白胨0.600%,KH2PO40.603%,KH2PO40.100%,MgSO4·7H2O0.050%,橄榄油0.500%,吐温-800.500%,初始pH值为7.85,转速为180r/min,28℃培养48h。最终优化后的酶活达到208.9U/ml,比初始酶活91.3U/ml提高了129%。 相似文献
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以羽毛为底物发酵产角蛋白酶培养基的优化 总被引：1，自引：0，他引：1
以角蛋白酶为考察指标,采用正交试验对以羽毛为底物,利用枯草芽孢杆菌菌株KD-N2生产角蛋白酶的培养基组成进行了优化试验.结果表明在NaCl含量为0.5 g/L,MgSO4含量为0.2 g/L,KH2PO4/K2HPO4含量为0.35/0.7 g/L时有利于角蛋白酶的产生,验证试验证明发酵24 h时角蛋白酶活性为(66.5±2.04)U/mL:各组发酵液pH值均呈上升趋势,发酵残留物重量也随发酵培养基组成不同而呈现一定的差异,表明以羽毛为底物发酵产角蛋白酶为一复杂过程.以天青角蛋白和酪蛋白为底物对酶活测定的结果表明了实验方法和结果的可靠性. 相似文献
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三株新型发酵产氢菌的分离和不同发酵条件下产氢性能的比较 总被引：1，自引：0，他引：1
氢能是理想的清洁能源,生物制氢技术的研究和开发已成为当前一个研究热点。许多细菌在厌氧条件下发酵复杂的有机化合物,同时产生H2。本文选择了来自同一活性污泥样品的3株有较高产氢能力的发酵细菌,采用生化和电镜技术对其形态学特征进行了观察比较,并进一步比较研究了在不同pH值条件下,它们产氢量、细菌生长量、液相发酵产物组成的变化和差异。获得了三株产氢菌的单位体积培养基最大累积产氢量,提出了纯培养生物制氢的最佳条件,为混合培养生物制氢强化工艺提供参考。 相似文献
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从受油烟污染严重的土壤中分离到一株脂肪酶产量较高的菌株，通过统计实验设计的方法对发酵过程进行了优化。首先，通过Plackett-Burman设计实验，对影响产脂肪酶发酵的碳源、氮源、无机盐、pH、温度和转速等因素进行考察。通过实验，发现黄豆粉、K2HPO4和摇床转速对脂肪酶的产生影响较大。随后，利用曲面响应法中的中心点设计进行实验，以上述3个因素作为自变量。以发酵液的酶活作为响应，通过拟合最终得到了脂肪酶发酵的响应曲面函数。对该函数在一定的约束条件下求极值．得到了脂肪酶产率最大时的发酵条件。 相似文献
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《中国科学院院刊(英文版)》2008,22(1):2-3
CAS should stick to the principle of rendering service to, and giving impetus for, the development of China＇s science enterprise by making S＆T innovations, said CAS President LU Yongxiang. The CAS president made the remarks in a recent talk to communicate the gist of the winter session of the Party＇s Leading-member Group at CAS, which was held from 7 to 11 January in Beijing. 相似文献
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《中国科学院院刊(英文版)》2008,22(1)
With great care, Dr. ZHOU Zhonghe takes out a package wrapped by cotton tissue from a drawer and says: "This is the gem of our collections: the fossil of a bird that lived 125 million years ago!" Then, pointing at a tiny mound, he explains: "Look, this is the claw and that is the head. It was in the egg shell and ready to hatch ... The species fell into a family of waterside inhabitants." 相似文献
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《中国科学院院刊(英文版)》2008,22(1):13-14
Aresearch project on the greenhouse cultivation of Swertia chirayita Buch Ham, a popular herbal drug used by the Tibetan people against liver and gallbladder diseases, recently passed the acceptance check by a panel of experts under the auspices of Qinghai Provincial S＆T Bureau. The feat was accomplished by researchers from the Lanzhoubased CAS Northwest Institute of Plateau Biology. 相似文献
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The research into the origins of domestic animals is of significance not only for understanding their development per se but also for making clear the human society evolution. Although there are evidences to show that pigs were independently domesticated in a variety of places throughout the world the detailed scenario of the origin dispersal of domestic pigs in East Asia remains unclear.…… 《中国科学院院刊(英文版)》2008,22(1):7-7
The research into the origins of domestic animals is of significance not only for understanding their development per se, but also for making clear the human society evolution. Although there are evidences to show that pigs were independently domesticated in a variety of places throughout the world, the detailed scenario of the origin and dispersal of domestic pigs in East Asia remains unclear. 相似文献
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Among the galaxy of research development organizations in northwest Beijing's Zhongguancun district known as China's Silicon Valley two are especially well-known to IT researchers: Microsoft Research Asia 《中国科学院院刊(英文版)》2008,22(1):47-49
Among the galaxy of research and development organizations in northwest Beijing＇s Zhongguancun district, known as China＇s Silicon Valley, two are especially well-known to IT researchers： Microsoft Research Asia （MSRA）, and, within a few blocks on the other side of the street, the CAS Institute of Automation （CASIA）. While most people in Chinese scientific organizations strive to move to a large foreign counterpart, Dr. Stan Z. Li （LI Ziqing） moves against the stream. 相似文献
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Prof. SUN Changpu from the CAS Institute of Theoretical Physics coworkers from University of Basel in Switzerland have worked out a way - at least in theory - to split a beam of molecules according to their chirality. The technique involves passing the molecules through three different laser beams is similar to the famous Stern-Gerlach effect whereby a beam of atoms passing through a magnetic field is split in two according to the atoms' spin states 《中国科学院院刊(英文版)》2008,22(1):23-23
Prof. SUN Changpu from the CAS Institute of Theoretical Physics and coworkers from University of Basel in Switzerland have worked out a way --at least in theory --to split a beam of molecules according to their chirality. The technique involves passing the molecules through three different laser beams and is similar to the famous Stern-Gerlach effect, whereby a beam of atoms passing through a magnetic field is split in two according to the atoms＇ spin states （Phys. Rev. Lett. 99 130403）. 相似文献
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《中国科学院院刊(英文版)》2014,(3):212-213
<正>In February 2014,the 30th Chinese Antarctic inland expedition team reported that they have successfully conducted the seeing measurement at the Antarctic Taishan Station using a Differential Image Motion Monitor(DIMM)developed by the Nanjing Institute of Astronomical Optic and Technology(NIAOT),Chinese Academy of Sciences.In astronomy,seeing is a physical parameter that describes the irregular movement and blur of the star 相似文献
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XIN Ling 《中国科学院院刊(英文版)》2014,(4):270-271
Amassive overhaul is coming to the Chinese Academy of Sciences （CAS）. To straighten its ＂fragmented, inefficient research＂, the academy has decided to restructure its 104 institutes for better teamwork. 相似文献
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SONG Jianlan 《中国科学院院刊(英文版)》2014,(4)
<正>DNA demethylation in mammalian zygotes,a biochemical process through which the parental genomes let go the epigenetic marks from their"past lives"to obtain totipotency,has long fascinated biologists:how does it happen?A joint team at the CAS Shanghai Institutes for Biological Sciences tells a surprising story. 相似文献
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China is one of the fastest growing economies worldwide. Are science and research also in the fast lane? We talked with LUO Yuan, scientist at the Institute of Coal Chemistry in Shanxi, and Axel Mosig, Professor at Ruhr University in Bochum, who has first-hand experience working and living in China. 相似文献