共查询到20条相似文献，搜索用时 15 毫秒
1.
Po Po THAN Haryudian PRIHASTUTI Sitthisack PHOULIVONG Paul W.J. TAYLOR Kevin D. HYDE 《Journal of Zhejiang University. Science. B》2008,9(10)
Anthracnose disease is one of the major economic constraints to chilli production worldwide, especially in tropical and subtropical regions. Accurate taxonomic information is necessary for effective disease control management. In the Colletotrichum patho-system, different Colletotrichum species can be associated with anthracnose of the same host. Little information is known concerning the interactions of the species associated with the chilli anthracnose although several Colletotrichum species have been reported as causal agents of chilli anthracnose disease worldwide. The ambiguous taxonomic status of Colletotrichum species has resulted in inaccurate identification which may cause practical problems in plant breeding and disease management. Although the management and control of anthracnose disease are still being extensively researched, commercial eultivars of Capsicum annuum that are resistant to the pathogens that cause chilli anthracnose have not yet been developed. This paper reviews the causal agents of chilli anthracnose, the disease cycle, conventional methods in identification of the pathogen and molecular approaches that have been used for the identification of Colletotrichum species. Pathogenetic variation and population structure of the causal agents of chilli anthracnose along with the current taxonomic status of Colletotrichum species are discussed. Future developments leading to the disease management strategies are suggested. 相似文献
2.
Young Soo Kim Kotnala Balaraju Yongho Jeon 《Journal of Zhejiang University. Science. B》2016,17(12):931-940
In this study, plant growth-promoting rhizobacteria (PGPR) were evaluated as potential biocontrol agents against postharvest pathogens of apple fruits. In vitro bioassays revealed that, out of 30 isolates screened, isolates APEC136 and APEC170 had the most significant inhibitory effects against the mycelial growth of several fungal pathogens. Analysis of 16S ribosomal RNA (rRNA) sequences identified the two effective isolates as Paenibacillus polymyxa and Bacillus subtilis, respectively. The two strains showed greater growth in brain-heart infusion broth than in other growth media. Treatment of harvested apples with suspensions of either strain reduced the symptoms of anthracnose disease caused by two fungal pathogens, Colletotrichum gloeosporioides and Colletotrichum acutatum, and white rot disease caused by Botryosphaeria dothidea. Increased productions of amylase and protease by APEC136, and increased productions of chitinase, amylase, and protease by APEC170 might have been responsible for inhibiting mycelial growth. The isolates caused a greater reduction in the growth of white rot than of anthracnose. These results indicate that the isolates APEC136 and APEC170 are promising agents for the biocontrol of anthracnose and white rot diseases in apples after harvest, and suggest that these isolates may be useful in controlling these diseases under field conditions. 相似文献
3.
炭疽菌的遗传转化及应用研究进展 总被引：1，自引：0，他引：1
由炭疽菌属（Colletotrichum cda．）真菌引起的植物炭疽病在生产上危害严重,近年来,炭疽菌转化技术的发展为全面研究该菌的致病机理及炭疽病防治奠定了良好的技术基础。本文重点介绍了炭疽菌的转化方法及在致病机理、致病相关基因克隆和病害防治等研究领域的应用。 相似文献
4.
从83个菌株中筛选出瓜类炭疽病菌弱致病株系C3、C5分别用其活体接种和其灭活物浇根,成功诱导了两瓜对疽病菌强致病株系C2的抗病性,并证明了弱致病株系的灭活物与其活体本身具有相近的诱导能力,同时试蛉得出了C2灭活物诱导抗性的持续期和诱导处理的最佳间隔期． 相似文献
5.
从药用植物大黄藤中分离出内生放线菌26株.以金黄色葡萄球菌、炭疽病菌和链格孢菌为受试菌株,对其进行抗菌活性筛选.结果表明,共有14株内生放线菌对1株或1株以上的受试菌株有抗菌活性,其中平板抑菌圈直径大于20mm的菌株有1株(ADG11).研究结果表明,大黄藤内生放线菌具有良好的潜在应用价值,值得做进一步研究. 相似文献
6.
高效液相色谱法测定草莓中维生素C含量 总被引：26，自引：0，他引：26
目的:建立一种高效液相色谱测定草莓中维生素C含量的新方法.方法:采用HYPERSIL-C18色谱柱,0.1%低浓度的草酸作流动相,流速为1.0 mL/m in,检测波长为254 nm.结果:维生素C在浓度为20~100 mg/L范围内有良好的线性关系,线性回归方程为y=9 361.6x-11 192,相关系数为0.999 3,方法回收率为97.4~102.1%,相对标准偏差小于3%.结论:本测定方法简便,快速,准确,稳定;适合于测定其它水果和蔬菜中维生素C的含量. 相似文献
7.
南阳市玉米穗腐病致病镰刀菌种群结构分析 总被引：1，自引：0，他引：1
镰刀菌是玉米穗腐病的主要病原菌.本研究对南阳市5县2009年夏玉米穗腐病致病镰刀菌的种群结构进行调查分析.从病样中按常规组织分离法进行病原菌分离,对分离物经单孢分离纯化后根据柯赫法则进行致病性检测,并根据形态特征和核糖体基因内转录间隔区（rDNA-ITS）序列对病原菌进行鉴定.从采集的80份穗腐病样品中分离获得105株镰刀菌,接种结果表明,所有菌株对玉米均有致病性.形态和分子鉴定结果表明,引起南阳市玉米穗腐病的镰刀菌至少有4种：层出镰刀菌Fusarium proliferatum、串珠赤霉菌Gibberella monoliformis、木贼镰刀菌F.equiseti及玉蜀黍赤霉菌G.zeae.南阳市玉米穗腐病致病镰刀菌以层出镰刀菌为优势种,串珠赤霉菌为次优势种. 相似文献
8.
杨会清 《赣南师范学院学报》2011,32(4):26-31
中国共产主义青年团作为中国共产党的得力助手和后备军,不仅是共产党领导和联系青年群众的桥梁和纽带,也是动员群众参加革命与建设的重要力量。不过,党团之间的这种关系并非一开始就是如此,而是随着革命形势的变化而逐渐形成的。土地革命时期党团关系的变迁,不仅让我们看到了党团关系调整的内在逻辑,而且为我们当代社会主义现代化建设背景下的党团关系建设提供了借鉴和启示。 相似文献
9.
原型重释——对当下原型定义的一点补充 总被引：1，自引：0，他引：1
张冰 《辽宁师范大学学报(社会科学版)》2003,26(4):88-90
国内近20年关于原型探讨主要是围绕着原型批评进行，这在一定程度上就忽略了荣格理论本身，并且荣格的原型理论与原型批评因分属于不同的学科领域，因而也不能将二简单的等同，基于此，重新梳理荣格的原型思想就成为一种必要。荣格对原型的阐释，不是确定的，而是不断发展和变化的，因而我们应该充分利用他对原型的每一次思考，将原型看作一个系统，来审视它的意义和存在价值。最后指出这种理解对中国艺理论的理论层面的启示有着十分积极的意义。 相似文献
10.
Than PP Prihastuti H Phoulivong S Taylor PW Hyde KD 《Journal of Zhejiang University. Science. B》2008,9(10):764-778
Anthracnose disease is one of the major economic constraints to chilli production worldwide, especially in tropical and subtropical
regions. Accurate taxonomic information is necessary for effective disease control management. In the Colletotrichum patho-system, different Colletotrichum species can be associated with anthracnose of the same host. Little information is known concerning the interactions of the
species associated with the chilli anthracnose although several Colletotrichum species have been reported as causal agents of chilli anthracnose disease worldwide. The ambiguous taxonomic status of Colletotrichum species has resulted in inaccurate identification which may cause practical problems in plant breeding and disease management.
Although the management and control of anthracnose disease are still being extensively researched, commercial cultivars of
Capsicum annuum that are resistant to the pathogens that cause chilli anthracnose have not yet been developed. This paper reviews the causal
agents of chilli anthracnose, the disease cycle, conventional methods in identification of the pathogen and molecular approaches
that have been used for the identification of Colletotrichum species. Pathogenetic variation and population structure of the causal agents of chilli anthracnose along with the current
taxonomic status of Colletotrichum species are discussed. Future developments leading to the disease management strategies are suggested.
相似文献
11.
研究了微波提取法从草莓中提取草莓红色素的工艺路线,通过单因素分析和正交实验设计确定了最佳工艺条件,并对草莓红色素的理化性质进行了进一步研究。结果表明,用无水乙醇作为提取剂,最佳提取工艺条件为:微波功率640 W,微波处理时间40 s,料液比1∶10。pH对色素的影响明显,在酸性条件下该色素较稳定,在碱性条件下出现浑浊显现。光照稳定性差,在温度60℃～80℃时吸光度最大。Fe2+,Cu2+对色素有明显的影响,而Na+,Mg2+,Zn2+,Ca2+对色素无不良影响。 相似文献
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14.
大学生党员保持党的先进性教育的探讨 总被引：1，自引：0，他引：1
杨丹 《黑龙江教育学院学报》2005,24(5):38-39
高等学校党组织应把握新形势,结合大学生自身特点与实际提高认识;研究新问题,有的放矢地开展教育活动;探索新方法,科学有效地促进教育活动的深入开展,为党的事业培养可靠的接班人。 相似文献
15.
建立自采草莓园要做好园地选择、品种选择和定植等工作.自采草莓园的田间管理工作包括施肥、摘除茎蔓、疏花疏果和病虫杂草防治等常规工作及采收期产品宣传和为顾客提供优质服务的特殊工作等. 相似文献
16.
周书勤 《安阳师范学院学报》2003,(1):7-8
解放思想、实事求是、与时俱进是党的十六大的精髓。高校要把党的十六大精神落到实处，在工作中首先要做到解放思想、实事求是、与时俱进。只有紧紧抓住十六大带来的新一轮思想解放的契机，全力推进教育创新，才能迎来素质教育的大发展、快发展，实现科教兴国的伟大战略。 相似文献
17.
柑橘炭疽病研究现状与展望 总被引：1，自引：0，他引：1
胡殿明 《赣南师范学院学报》2010,31(3):87-90
柑橘炭疽病是一种常见的,为害较为严重的真菌性病害,其病原菌是Colletotrichum gloeosporioides Penz.柑橘炭疽病一般在夏秋季节,高温多雨的条件下较容易发病.目前对柑橘炭疽病的防治方法主要是进行合理的田间管理和使用合适的化学农药.本文基于近期关于柑橘炭疽病的研究报道,对柑橘炭疽病的病症及为害、病原体、发病规律、防治等几个方面进行了系统的综述.对近期柑橘炭疽病的研究成果进行了简要的小结,提出了今后柑橘炭疽病研究中需要解决的问题及其发展趋势. 相似文献
18.
茄子皮天然红色素的提取及稳定性的研究 总被引：4，自引：0，他引：4
以茄子皮为原料,用酸性无水乙醇溶液浸泡提取天然红色素,并通过实验对色素溶液在不同pH值下在温度,光照,氧化还原剂,金属离子的稳定性进行了研究.实验证明:茄子皮天然红色素可以适用大多数食品,是一种值得开发的天然食用色素资源. 相似文献
19.
Chen-jian Liu Fu-ming Gong Xiao-ran Li Hai-yan Li Zhong-hua Zhang Yue Feng Hiroko Nagano 《Journal of Zhejiang University. Science. B》2012,13(4):298-306
This research was aimed at isolating and identifying the predominant lactic acid bacteria (LAB) in the traditional Chinese
salt-fermented soybean food, douchi, from Yunnan, China. The predominant LAB present were isolated and identified by conventional
culture-dependent methods combined with molecular biological methods. Two hundred and sixty isolates were obtained from thirty
kinds of traditional fermented douchi from six cities and counties in Yunnan, and those strains were divided into twelve groups
by their morphological and biochemical characteristics. Based on 16S ribosomal DNA (rDNA) sequencing and analysis, 56 representative
strains were identified as belonging to 6 genera and 14 species: Lactobacillus (4 spp.), Weissella (3 spp.), Pediococcus (2 spp.), Staphylococcus (2 spp.), Enterococcus (1 sp.), and Bacillus (2 spp.). The results show that douchi contains a large natural population of LAB of diverse composition from which some
strains could be selected as starters for functional fermented foods. This is the first study on the original douchi from
Yunnan, and the results suggest that it may be a useful source for the isolation of LAB. This study has also laid a foundation
for further research on developing functional douchi products. 相似文献
20.
20世纪20年代,李大钊的统战思想经历了从最初的“民众大联合”,到建立与资产阶级民主派等其他各阶级的联合,到明确阐述国共合作的必要性及联合策略,再到坚持和维护共产党在统一战线中的领导权这四个阶段的演变,从而奠定了中共统战思想的基础。李大钊的统战思想,代表了当时的中国共产党对这个问题认识的最高水平,集中体现了中共把马列主义统一战线理论与中国实际相结合的早期成果,在中共的统战思想史上占有重要地位。 相似文献