共查询到20条相似文献，搜索用时 31 毫秒
1.
Objective: To study the changes of oxidative metabolism and lipoperoxidative metabolism in patients with acute viral myocarditis
(AVM), and to research pathological chain reactions of a series of free radicals, and oxidative damage and lipoperoxidative
damage in AVM patients' bodies. Methods: A random paired control design was used to investigate 70 cases of AVM patients and
70 cases of healthy adult volunteers (HAV) on the basis of contents of nitric oxide (NO), lipoperoxide (LPO) in plasma, and
LPO in red blood cell (RBC) as well as activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase
(GSH-Px) in RBC as revealed by spectrophotometric assays. Results: Compared with the average values (AV) of the above biochemical
parameters (BP) in the HAV group, the AV of NO in plasma, and LPO in plasma and in RBC in the AVM group were significantly
increased (P=0.0001), while the AV of SOD, CAT and GSH-Px in the AVM group were significantly decreased (P=0.0001). Conclusion: The findings in this study suggested that in the AVM patients bodies the metabolism of NO was disturbed,
and the pathological chain reactions of a series of free radicals were so severely aggravated, as to cause the oxidative damage
and lipoperoxidative damage in the AVM patients' bodies. 相似文献
2.
CHANGES OF OXIDATIVE AND LIPOPEROXIDATIVE METABOLISM IN PATIENTS WITH ACUTE VIRAL MYOCARDITIS 总被引：6，自引：0，他引：6
ＩＮＴＲＯＤＵＣＴＩＯＮＭｙｏｃａｒｄｉｔｉｓｉｓａｃｏｍｍｏｎｃａｒｄｉａｃｄｉｓｅａｓｅ.Ｔｈｅｒｅａｒｅｒｅｐｏｒｔｓｔｈａｔａｃｔｉｖｉｔｉｅｓｏｆｓｕｐｅｒｏｘｉｄｅｄｉｓ ｍｕｔａｓｅａｎｄｃａｔａｌａｓｅｉｎｂｌｏｏｄｏｆｖｉｒａｌｍｙｏｃａｒｄｉｔｉｓｐａｔｉｅｎｔｓａｎｄａｎｉｍａｌｓｐｒｏｄｕｃｅｄｓｏｍｅｃｈａｎｇｅｓ,ａｎｄａｇｇｒａｖａｔｅｄｒｅａｃｔｉｏｎｓｏｆｆｒｅｅｒａｄｉｃａｌｓｉｎｔｈｅｉｒｂｏｄｉｅｓ (Ｈｉｒａｏｋａｅｔａｌ.,1 992 ,1 993 ,1 995;Ｓｕｚｕｋｉｅ… 相似文献
3.
Salvianolic acid B(Sal B) is an active component of traditional Chinese medicine Salvia miltiorrhiza and is used to treat vascular diseases. To better understand its mechanism, the antioxidant capacities of Sal B was evaluated with human endothelial cells under oxidative stress. Human endothelial cells were pretreated with Sal B for 12 h followed by hydrogen peroxide for another 12 h. Production of reactive oxygen species (ROS), activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), and concentration of glu-tathione were measured: Protective effect of Sal B on the endothelial cells from hydrogen peroxide-induced damage ' was observed, and ROS production in the cells was found significantly inhibited. Sal B remarkably enhanced the activities of antioxidant enzymes SOD, CAT and GPX. Furthermore, Sal B up-regulated the intracellular glutathione concentration. The results indicate that Sal B protected endothelial cells from oxidative stress by improving the redox status of the cells through enhancing the antioxidant enzyme activities and increasing the reductive glutathione concentration after the oxidative challenge. 相似文献
4.
采用小麦幼苗为材料，研究了冷激诱导抗冷性过程中超氧化物歧化酶（SOD），过氧化氢酶（CAT），抗坏血酸专一性过氧化物酶（APX）、愈创木酚过氧化物酶（GPX）四种抗氧化酶活性的变化，结果发现冷激可以提高SOD，CAT，APX，GPX四种抗氧化酶的活性，表明冷激诱导的抗冷性与SOD，CAT，APX，GPX四种抗氧化酶活性增加有关，暗示SOD，CAT，APX，GPX四种抗氧化酶在冷激诱导小麦幼苗抗冷性的形成过程中起重要作用。 相似文献
5.
目的以衰老的自由基学说为依据,研究蚁皇浆口服液的抗衰老作用.方法52周龄小白鼠30只随机分为老龄实验组和老龄对照组,4周龄15只作为成年对照组,灌胃四周后分别取血测定SOD、CAT、Tch、TG和MDA等各项生化指标.结果老龄实验组SOD、CAT活性均明显升高(P＜0.01),MDA、Tch和TG含量明显降低(P＜0.01或P＜0.05).结论蚁皇浆口服液具有较强的抗氧化和抗衰老作用,是一种很有经济价值药食两用的保健品. 相似文献
6.
Leaf senescence is often caused by water deficit and the chimeric gene PSA612-1PT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance （TBARS） and antioxidant enzymes activities were investigated during leaf senescence of PSA612-1PT modified gerbera induced by osmotic stress compared with the control plant （wild type）. Leaf discs were incubated in 20%, 40% （w/v） polyethylene glycol （PEG） 6 000 nutrient solution for 20 h under continuous light [ 130 μmol/（m^2·s）]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase （SOD）, catalases （CAT）, ascorbate peroxidase （APX）, guaiacol peroxidase （GPX） and dehydroascorbate reductase （DHAR） were stimulated by PEG treatment. However, the increases were higher in PSA612-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation （TBARS content） was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities ofantioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress. 相似文献
7.
姜黄素对小鼠抗氧化酶活性及NO含量的影响 总被引：4，自引：0，他引：4
选取60只小鼠,随机分成四组(A、B、C、D),A组为对照组,饲喂基础饲料;B、C、D组分别在基础饲料中添加0.02%、0.04%、0.06%的姜黄素.正常饲养15d后,屠宰测定小鼠血清、肝脏、心脏、脑组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性及丙二醛(MDA)、NO的含量.结果表明,小鼠采食添加姜黄素的饲料后,体内抗氧化酶SOD、CAT活性升高,MDA、NO含量下降. 相似文献
8.
目的:探讨沙参麦冬汤对慢性支气管炎大鼠肺、脑组织抗氧化能力的影响.方法:70只SD大鼠,随机分为对照组、慢支模型组、沙参麦冬汤高、低剂量组和氨茶碱组,采用混合烟熏方法复制慢性支气管炎大鼠动物模型,对照组与慢支模型组灌服生理盐水10 mL/kg·d,高剂量组灌服沙参麦冬汤10 mL/kg·d,低剂量组灌服沙参麦冬汤5 mL/kg·d,氨茶碱组灌服氨茶碱0.25 g/kg·d,灌胃期30 d.实验结束后,测定体重和肺指数,测定肺、脑组织中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢(CAT)的活力和丙二醛(MDA)的含量.结果:各组的肺指数差异均不显著(P>0.05),沙参麦冬汤对慢支大鼠肺、脑的CAT活性无明显影响(P>0.05),但能显著提高肺、脑组织中SOD,GSH-Px的活性(P<0.05),显著降低肺、脑MDA的含量(P<0.05).结论:沙参麦冬汤可以提高慢支大鼠肺、脑组织的抗氧化能力,降低烟雾造成的氧化损伤. 相似文献
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10.
灯盏花素治疗冠心病的疗效观察 总被引：5，自引：0，他引：5
目的：探讨灯盏花素治疗冠心病的疗效机理．方法：用灯盏花素治疗冠心病伴高粘滞血症患者４０例，与用复方丹参注射液治疗的同样患者４０例进行对比，两组疗程均１５天．对血清脂质过氧化物（ＬＰＤ）含量、超氧化物歧化酶活性（ＳＯＤ）、血脂、血液流变学指标进行检测．结果：患者症状、心电图、血脂、血流变、ＬＰＯ、ＳＯＤ，治疗组较对照组改善明显．（ｐ＜０．０５）．结论：灯盏花素降低冠心病者血液的高粘滞状态，提高抗氧化能力，有效改善其症状． 相似文献
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12.
Lipid peroxide (LPO) plays pivotal roles in the process and development of many diseases. In this work, we studied the inhibitory effect of probimane (Pro), a Chinese anticancer agent, on erythrocyte LPO and the interaction of Pro with sialic acids (sia). Malondialdehyde (MDA) of erythrocytes activated by hydrogen peroxide was measured. Pro was found to inhibit the product of LPO induced by hydrogen peroxide in a non-enzyme system of both rabbit and human erythrocytes in the absence of doxorubicin. Sia were 相似文献
13.
Qian-nan Diao Yong-jun Song Dong-mei Shi Hong-yan Qi 《Journal of Zhejiang University. Science. B》2016,17(12):916-930
Polyamines (PAs) and nitric oxide (NO) are vital signals in modulating plant response to abiotic stress. However, to our knowledge, studies on the relationship between NO and PAs in response to cold stress in tomato are limited. Accordingly, in this study, we investigated the effects of putrescine (Put) and spermidine (Spd) on NO generation and the function of Spd-induced NO in the tolerance of tomato seedling under chilling stress. Spd increased NO release via the nitric oxide synthase (NOS)-like and nitrate reductase (NR) enzymatic pathways in the seedlings, whereas Put had no such effect. Moreover, H2O2 might act as an upstream signal to stimulate NO production. Both exogenous NO donor (sodium nitroprusside (SNP)) and Spd enhanced chilling tolerance in tomato, thereby protecting the photosynthetic system from damage. Compared to chilling treatment alone, Spd enhanced the gene expressions of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and their enzyme activities in tomato leaves. However, a scavenger or inhibitor of NO abolished Spd-induced chilling tolerance and blocked the increased expression and activity due to Spd of these antioxidant enzymes in tomato leaves under chilling stress. The results showed that NO induced by Spd plays a crucial role in tomato’s response to chilling stress. 相似文献
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15.
人参抗衰老作用的实验研究 总被引：4，自引：0，他引：4
徐承水 《赣南师范学院学报》2000,(3):54-56
采用人参水提取液 ,对小鼠的试验研究表明 :人参提取物能有效提高心、脑组织超氧化物歧化酶 (SOD)活性 ,降低过氧化脂质 (LP0 )和脂褐素含量 ,拮抗过氧化损伤 ,具有明显的抗衰老作用 相似文献
16.
以福寿螺为研究对象,测定其在Zn^2＋浓度分别为0、1、2、5、10mg／L时,于不同暴露时间（12、24、48、96h）内,肝脏超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和丙二醛（MDA）活性的变化．结果表明：低浓度（1、2mg／L）的Zn^2＋处理组在24h内对福寿螺肝脏SOD活性起到诱导作用;随着时间的延长活性逐渐减弱,在96h时下降至最低值,极显著低于对照组水平;而中、高浓度（5、10mg／L）处理组酶活性均低于对照组．在各浓度组的胁迫下,CAT活性在12h时升高到最大值,之后随着时间的延长均表现为下降趋势．MDA含量在整个试验期间均处于诱导状态,并且与重金属浓度成正比,在10mg／L96h时达到最大值．Zn^2＋会对福寿螺产生明显的氧化胁迫,SOD、CAT和MDA指标能有效评价Zn^2＋对福寿螺的氧化损伤． 相似文献
17.
探讨了全氟辛烷磺酸盐(PFOS)对斑马鱼胚胎的发育毒性与氧化应激的影响。将斑马鱼胚胎暴露于系列浓度(0.1mg/L,0.5mg/L,1mg/L,5mg/L,10mg/L)的PFOS中,分别在胚胎受精后数小时(24hpf,48hpf,72hpf,96hpf,120hpf)内统计急性发育毒性指标,包括存活率、畸形率、心率以及孵化率。同时在暴露结束的120hpf,检测相关的抗氧化酶活性变化,主要包括超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(Gpx)以及脂质过氧化物(MDA)。结果显示,PFOS能够导致胚胎/幼鱼存活率降低,畸形率增加,心率加快以及孵化延迟;同时,胚胎SOD活性以及MDA水平增加,CAT与Gpx活性降低,表明PFOS能够诱发斑马鱼胚胎急性发育中毒,影响其抗氧化系统,导致胚胎产生氧化应激。 相似文献
18.
Hong-zhou Meng Xiao-feng Ni Hai-ning Yu Shan-shan Wang Sheng-rong Shen 《Journal of Zhejiang University. Science. B》2017,18(2):161-171
Astaxanthin (AST), a carotenoid molecule extensively found in marine organisms and increasingly used as a dietary supplement, has been reported to have beneficial effects against oxidative stress. In the current paper, the effects of AST on viability of prostate cells were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay; cell apoptosis and intracellular reactive oxygen species (ROS) levels were determined by flow cytometry; the mitochondrial membrane potential (MMP) was measured by fluorospectrophotometer; and activities of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were evaluated by a detection kit. The results show that copper ion (Cu2+) induced apoptosis, along with the accumulation of intracellular ROS and MDA, in both prostate cell lines (RWPE-1 and PC-3). AST treatments could decrease the MDA levels, increase MMP, and keep ROS stable in RWPE-1 cell line. An addition of AST decreased the SOD, GSH-Px, and CAT activities in PC-3 cell line treated with Cu2+, but had a contrary reaction in RWPE-1 cell lines. In conclusion, AST could contribute to protecting RWPE-1 cells against Cu2+-induced injuries but could cause damage to the antioxidant enzyme system in PC-3 cells. 相似文献
19.
贾文娟 《思茅师范高等专科学校学报》2013,(6):13-18
目的：通过实验观察甲醛对运动大鼠心肌组织抗氧化系统的影响及服用刺五加后的作用,探讨甲醛对运动大鼠的心肌组织毒性及刺五加的保护作用。方法：采用人为模拟不同程度甲醛染毒环境进行动式染毒,甲醛剂量分别为0.8 mg/m3,2.4 mg/m3。每天染毒30 min,每周6次,连续4周,刺五加的灌胃剂量为104.17 mg/kg,测定运动大鼠心肌组织的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）的活力和丙二醛（MDA）的含量。结果：染毒组与对照组相比,2.4 mg/m3剂量染毒运动组大鼠心肌组织 CAT、GSH-PX、SOD活性明显低于对照组（P〈0.01）,MDA含量明显高于对照组（P〈0.01）。服药染毒运动组与染毒运动组相比,0.8mg/m3剂量染毒服药组大鼠心肌组织CAT、GSH-PX、SOD活性明显高于染毒运动组（P〈0.01）,MDA含量明显低于染毒运动组（P〈0.01）。结论：甲醛可以降低染毒大鼠心肌组织抗氧化酶的活性,造成心肌组织氧化性损伤,而灌服中药刺五加可以有效缓解其毒性。 相似文献
20.
Zhang DL Mi YL Wang KM Zeng WD Zhang CQ 《Journal of Zhejiang University. Science. B》2008,9(7):567-571
The attenuating effect of daidzein (DAD on oxidative toxicity induced by Aroclor 1254 (A 1254) was investigated in mouse testicular cells. Cells were exposed to A1254 alone or with DAI. The oxidative damage was estimated by measuring malondialdehyde (MDA) formation, superoxide dismutase (SOD) activity and glutathione (GSH) content. Results show that A 1254 induced a decrease of germ cell number, an elevation in thiobarbituric acid reactive substances (TBARS) but a decrease in SOD activity and GSH content. However, simultaneous supplementation with DAI decreased TBARS level and increased SOD activity and GSH content. Consequently, dietary DAI may restore the intracellular antioxidant system to attenuate the oxidative toxicity of A1254 in testicular cells. 相似文献