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茶皂素对福寿螺纤维素酶的影响研究 总被引:2,自引:0,他引:2
从苦茶粕中提取茶皂素,用离体的方法测定它对福寿螺中消化酶-纤维素酶活性的影响.结果表明,在所测定的10-150μg/ml的浓度内,茶皂素对内切β-1,4葡聚糖酶(EG)的作用表现为先激活,后抑制;对外切β-1,4葡聚糖纤维二糖水解酶(CBH)的作用表现为激活作用,基本上不表现抑制作用. 相似文献
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纤维素酶研究进展 总被引:3,自引:0,他引:3
赵荣乐 《喀什师范学院学报》2005,26(6):51-54
对纤维素利用中的核心热点问题——纤维素酶的生态分布、酶的组成、纤维素酶活性功能区、纤维素酶的作用机理、纤维素酶的表达调控的基础研究和纤维素酶的实际应用研究的进展作了综合评述. 相似文献
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采用正交实验法分析出绿色木霉 3.3711纤素酶系各组分最适酶解条件组合分别为(pH5 .0、4 5℃ ) (C1酶 ) ,(pH3.5、5 5℃ ) (Cx 酶 )和 (pH4 .5、6 5℃ ) (βG酶 )。分析比较了分别使用纤维素粉和麦麸为单一或混合碳源时在产酶过程中各组分酶的最高酶活 ,比酶活及其形成时间和分泌特性。结果表明 ,同种碳源不同浓度所产酶系中C1比酶活相对稳定 ,C1和Cx 酶活分别形成一个和两个高峰 ,βG酶均为一个高峰。混合碳源使各组分酶活高峰期相对一致 ,高峰期稳定时间延长和酶量增加。 相似文献
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以枇杷花为原料、黄酮类物质含量为评价指标,考察纤维素酶用量、料液比、提取时间、提取温度和超声波功率等对枇杷花黄酮类物质提取效果的影响。结果表明,酶法结合超声波技术提取枇杷花黄酮类物质最佳工艺条件为:配制料液比1:50,先经纤维素酶水解再经超声波处理,纤维素酶用量100mg,酶解温度60℃,酶解时间30min,超声波功率250W、提取时间20min,黄酮类物质得率为8.37%。 相似文献
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BackgroundAt present, cellulases are the most important enzymes worldwide, and their demand has been increasing in the industrial sector owing to their notable hydrolysis capability.ResultsIn the present study, contrary to conventional techniques, three physical parameters were statistically optimized for the production of cellulase by thermophilic fungi by using response surface methodology (RSM). Among all the tested thermophilic strains, the best cellulase producing fungus was identified as Talaromyces thermophilus – both morphologically and molecularly through 5.8S/ITS rDNA sequencing. The central composite design (CCD) was used to evaluate the interactive effect of the significant factors. The CCD was applied by considering incubation period, pH, and temperature as the model factors for the present investigation. A second-order quadratic model and response surface method revealed that the independent variables including pH 6, temperature 50 °C, and incubation period 72 h significantly influenced the production of cellulases. The analysis of variance (ANOVA) indicated that the established model was significant (P ≤ 0.05) and showed the high adequacy of the model. The actual and predicted values of CMCase and FPase activity showed good agreement with each other and also confirmed the validity of the designed model.ConclusionsWe believe the present findings to be the first report on cellulase production by exploiting Kans grass (Saccharum spontaneum) as a substrate through response surface methodology by using thermophilic fungus, Talaromyces thermophilus.How to cite: Abdullah R, Tahseen M, Nisar K et al. Statistical optimization of cellulases by Talaromyces thermophilus utilizing Saccharum spontaneum, a novel substrate. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.03.007. 相似文献
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纤维素降解过程中金属离子作用的研究 总被引:6,自引:0,他引:6
用微量量热法研究了7种金属离子及几种混合离子对纤维素酶降解纤维素反应的影响.实验结果表明,在pH=4.60,T=310K时,相同浓度的离子中Li+、Na+、K+、Ni2+、Zn2+、Co2+离子均表现出抑制作用,其中Li+抑制作用显著;Cu2+有明显的激活作用.当Li+、Na+、K+3种离子混合共同作用于反应时,表现出激活作用;当Ni2+、Cu2+、Zn2+3种离子,Co2+、Ni2+2种离子混合共同作用于反应时,表现出抑制作用. 相似文献
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从废弃菌包中分离得到的11株菌株通过固体培养初筛和摇瓶发酵复筛,筛选出纤维素酶活性较高的2株菌株,分别记为f-6和f-10菌株。f-6菌株的Cx酶活力最高,作用于可溶性纤维素的效率最高;f-10菌株的复合酶活力最高,作用于不溶性纤维素的效率最高。在实际应用中,作用于可溶性的纤维素应该考虑选用菌株f-6,作用于不溶性的纤维素则考虑选用菌株f-10。 相似文献