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1.
Lu Yuan-qiang Cai Xiu-jun Gu Lin-hui Fan Yu-jing Wang Qi Bao De-guo 《浙江大学学报(A卷英文版)》2005,6(9):907-912
Objective: To observe the effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic
shock. Methods: A model of rat with severe hemorrhagic shock and active bleeding was established in 32 SD (Sprague-Dawley)
rats. The rats were randomly divided into control group, no fluid resuscitation group (NF group), controlled fluid resuscitation
group (NS40 group) and rapid large scale fluid resuscitation group (NS80 group). Each group contained 8 rats. The curative
effects were compared. At the same time, the apoptosis in liver, kidney, lung and small intestinal mucosa of survivors after
hemorrhage and resuscitation was detected by light microscopy in HE (hematoxylin and eosin) stained tissue sections, flow
cytometry and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Results: The survival rate of early fluid
resuscitation (14/16) was markedly higher than that of NF group (3/8). There was some apoptosis in liver, kidney, lung and
small intestinal mucosa of all survivors. Compared with NF and NS40 groups, the apoptosis of liver, kidney and small intestinal
mucosa of NS80 group was obviously increased. Conclusions: Among three fluid resuscitation methods, controlled fluid resuscitation
can obviously improve the early survival rate and the apoptosis of liver, kidney and small intestinal mucosa in rats with
severe and uncontrolled hemorrhagic shock, and may benefit improvement of prognosis. 相似文献
2.
Hypertonic saline resuscitation reduces apoptosis of intestinal mucosa in a rat model of hemorrhagic shock 总被引：1，自引：0，他引：1
Objective: To investigate the early effects of hypertonic and isotonic saline solutions on apoptosis of intestinal mueosa in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 21 Spragne-Dawley (SD) rats. The rats were randomly divided into the sham group, normal saline resuscitation (NS) group, and hypertonic saline resuscitation (HTS) group, with 7 in each group. We detected and compared the apoptosis in small intestinal mucosa of rats after hemorrhagic shock and resuscitation by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), FITC (fluo-rescein-iso-tbiocyanate)-Annexin V/PI (propidium iodide) double staining method, and flow cytometry. Results: In the early stage of hemorrhagic shock and resuscitation, marked apoptosis of small intestinal mucosa in the rats of both NS and HTS groups was observed. The numbers of apoptotic cells in these two groups were significantly greater than that in the sham group (P＜0.01). In the HTS group, the apoptic cells significantly decreased, compared with the NS group (P＜0.01). Conclusion: In this rat model of severe hemorrhagic shock, the HTS resuscitation of small volume is more effective than the NS resuscitation in reducing apoptosis of intestinal mucosa in rats, which may improve the prognosis of trauma. 相似文献
3.
Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock 总被引：5，自引：0，他引：5
Objective： To investigate the potential and early effect of hypertonic saline resuscitation on T-lymphocyte subpopulations in rats with hemorrhagic shock. Methods： A model of rat with severe hemorrhagic shock was established in 18 Sprague-Dawley （SD） rats. The rats were randomly divided into Sham group, HTS group （hypertonic saline resuscitation group） and NS group （normal saline resuscitation group）. Each group contained 6 rats. The CD4~ and CD8~ subpopulations of T-lymphocytes in peripheral blood were detected respectively before shock and after resuscitation by double antibody labelling and flow cytometry. Results： In the early stage after hemorrhagic shock, fluid resuscitation and emergency treatment, the CD4^＋ lymphocytes of peripheral blood in HTS and NS groups markedly increased. Small volume resuscitation with HTS also induced peripheral CD8^＋ lymphocytes to a certain extent, whereas NS resuscitation showed no effect in this respect. Consequently, compared with Sham and HTS groups, CD4^＋/CD8^＋ ratio of peripheral blood in NS group was obviously increased, and showed statistically differences. Conclusion： In this model of rat with severe hemorrhagic shock, small volume resuscitation with HTS is more effective than NS in reducing immunologic disorders and promoting a more balanced profile of T-lymphocyte subpopulations regulating network. 相似文献
4.
目的通过肺组织NF-κB的表达,研究羟乙基淀粉130/0.4复苏对失血性休克大鼠肺损伤保护作用的可能机制.方法健康雄性SD大鼠54只,体重230~280 g,随机分为3组：对照组（C组）、乳酸林格氏液复苏组（RL组）、6%羟乙基淀粉130/0.4复苏组（HES组）,按照Wiggers改良法复制失血性休克模型.于复苏后1 h、2 h4、h采用免疫组织化学的方法检测肺组织NF-κB的表达;光镜下观察肺组织形态学改变.结果 RL组与HES组肺组织NF-κB免疫组化染色细胞核阳性百分率较C组明显升高,RL组升高的程度较HES组明显（P〈0.01）.免疫组化染色光镜观察显示：HES组比RL组的组织损伤明显减轻.结论 6%羟乙基淀粉130/0.4复苏失血性休克可以抑制肺组织NF-κB活性,减轻休克及复苏过程造成的肺损伤. 相似文献
5.
目的:探讨川芎嗪、当归注射液对失血性休克大鼠血液动力学的影响。方法:颈动脉放血复制大鼠失血性休克模型,通过微循环观察、器官血流和血液流变学测定方法,观察川芎嗪、当归注射液对失血性休克大鼠血液动力学的影响。结果:失血性休克时,微循环明显障碍,主要表现为微血流变慢和血细胞聚集;经川芎嗪、当归注射液治疗后,微循环改善,血粘度、血小板粘附率和聚集率降低,器官微区血流量增加,与NS组比较有显著性差异(P<0.05)。结论:川芎嗪、当归注射液能明显改善失血性休克大鼠微循环障碍,降低血小板聚集率,增加器官血液灌流量。 相似文献
6.
Zhang Y Liang ZY Zhang SY Huang FF Wu W Gao Y Chen ZB 《Journal of Zhejiang University. Science. B》2008,9(11):871-878
Objective: To determine the effects of albumin administration on lung injury and apoptosis in traumatic/hemorrhagic shock (T/HS) rats. Methods: Studies were performed on an in vivo model of spontaneously breathing rats with induced T/HS; the rats were subjected to femur fracture, ischemia for 30 min, and reperfusion for 20 min with Ringer’s lactate solution (RS) or 5% (w/v) albumin (ALB), and the left lower lobes of the lungs were resected. Results: Albumin administered during reperfusion markedly attenuate... 相似文献
7.
Xing HC Li LJ Xu KJ Shen T Chen YB Sheng JF Yu YS Chen YG 《Journal of Zhejiang University. Science. B》2005,6(1):14-21
Objectives:To investigate the intestinal microflora status related to ischemia/reperfusion(I/R)liver injury and explorethe possible mechanism.Methods:Specific pathogen free grade Sprague-Dawley rats were randomized into three groups:Controlgroup(n=8),sham group(n=6)and I/R group(n=10).Rats in the control group did not receive any treatment,rats in the I/R groupwere subjected to 20 min of liver ischemia,and rats in the sham group were only subjected to sham operation.Twenty-two hourslater,the rats were sacrificed and liver enzymes and malondialdehyde(MDA),superoxide dismutase(SOD),serum endotoxin,intestinal bacterial counts,intestinal mucosal histology,bacterial translocation to mesenteric lymph nodes,liver,spleen,andkidney were studied.Results:Ischemia/reperfusion increased liver enzymes,MDA,decreased SOD,and was associated withplasma endotoxin elevation in the I/R group campared to those in the sham group.Intestinal Bifidobacteria and Lactobacillidecreased and intestinal Enterobacterium and Enterococcus 相似文献
8.
为了探讨失血性休克不同时期淋巴循环的变化,本文采用肠淋巴管插管技术对失血性休克大鼠不同时期的肠淋巴流量及肠淋巴管压力进行了测定。结果表明,失血性休克不同时期肠淋巴循环发生了明显变化。休克早期、休克晚期的肠淋巴流量和肠淋巴管压力明显降低,且显著低于对照组(P<0.05-0.01);(输血输液治疗后,肠淋巴流量和肠淋巴管压力迅速回升并显著高于对照组(P<0.05-0.01)。结果提示,失血性休克的发生、发展及转归与淋巴循环的变化密切相关。 相似文献
9.
Feng Yao Yuan-qiang Lu Jiu-kun Jiang Lin-hui Gu Han-zhou Mou 《Journal of Zhejiang University. Science. B》2017,18(5):402-409
Objective
To investigate the effects of resuscitation with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES) on regulatory T cells (Tregs), helper T 1 (Th1)/Th2 and cytotoxic T 1 (Tc1)/Tc2 profiles in the treatment of hemorrhagic shock.Methods
Rats subjected to severe hemorrhagic shock were resuscitated for 30 min with NS (n=8), HTS (n=8), or HES (n=8); sham (n=8) and naive control (n=8) groups were used for comparison. Following fluid resuscitation, the whole shed blood was reinfused for 30 min, and the rats were observed with continuous hemodynamic monitoring for 120 min. CD4+CD25+Foxp3+ Treg proportions, Th1/Th2 and Tc1/Tc2 profiles in spleen were analyzed by three-color flow cytometry.Results
The proportion of CD4+CD25+Foxp3+ Tregs and ratios of Th1/Th2 and Tc1/Tc2 did not differ among control, sham, and HTS groups, but were significantly lower in NS and HES groups (both P<0.05 vs. sham); NS and HES levels were similar. The level of Tc1 was significantly increased in HTS (P<0.05 vs. sham), and levels of Tc2 were increased in NS, HES, and HTS groups compared to sham (all P<0.05), but did not differ from each other.Conclusions
HTS resuscitation has a greater impact on immune system recovery than NS or HES by preserving the proportion of Tregs and maintaining the balance between Th1/Th2 and Tc1/Tc2 cells in the spleen. Thus, HTS resuscitation provides potential immunomodulatory activity in the early stage after hemorrhagic shock.10.
中草药提取物四黄素对鸡小肠黏膜内淋巴细胞分布的影响 总被引：1，自引：0，他引：1
研究中草药提取物四黄素对鸡小肠黏膜内淋巴细胞分布的影响.试验选用160羽1日龄健康雏鸡,随机分为两组,试验组和对照组,每组80羽.中草药提取物四黄素按0.6%添加到试验组鸡的基础日粮中.试验期40d.试验结束时,每组随机选取24羽鸡进行屠宰,采集鸡小肠组织样本.用组织学方法观察中草药提取物四黄素对鸡小肠黏膜内淋巴细胞分布的影响.试验组鸡十二指肠黏膜内淋巴细胞分布较对照组明显增多,且淋巴细胞有向黏膜腔排放的现象;试验组鸡空肠黏膜内的淋巴细胞分布较对照组更加密集,试验组鸡回肠黏膜和黏膜下层中淋巴细胞有明显聚集现象,而对照组鸡回肠黏膜和黏膜下层中淋巴细胞数量较试验组少.中草药提取物四黄素作为饲料添加剂对鸡小肠黏膜免疫具有明显的促进作用. 相似文献
11.
本实验采用肠淋巴管引流术,在失血性休克大鼠模型的基础上,观察高渗盐水治疗失血性休克过程中肠淋巴流量及其蛋白含量的变化,旨在探讨高渗盐水对失血性休克大鼠治疗的淋巴机制。结果表明,休克大鼠输入高渗盐水或生理盐水后,两组大鼠的血压、肠淋巴流量及其蛋白输出量均比休克期明显升高,高渗盐水治疗组显著高于生理盐水对照组(P<0.01),而且治疗组大鼠肠淋巴流量及其蛋白输出量远远高于休克前水平(P<0.01)。结果提示,高渗盐水的抗休克机制可能与其恢复休克大鼠的淋巴流量及其蛋白输出量、改善休克时肠淋巴循环障碍有关。 相似文献
12.
以少量胸导管淋巴液和肠淋巴液给予补液干予治疗的重症失血性休克大鼠,用生理盐水做对照,观察其对血压及存活时间的影响,以探讨不同部位淋巴液维持血压的共同特性。结果表明:胸导管淋巴液及肠淋巴液回升血压及延长存活时间作用均显著优于对照组(P<0.05～0.01),而两个治疗组间未见统计学差异(P>0.05)。结果提示:胸导管淋巴液和肠淋巴液对失血性休克大鼠均有较好的抗休克作用。 相似文献
13.
Jiu-kun Jiang Wen Fang Liang-jie Hong Yuan-qiang Lu 《Journal of Zhejiang University. Science. B》2017,18(1):48-58
Objective
To investigate the distribution and differentiation of myeloid-derived suppressor cells (MDSCs) in hemorrhagic shock mice, which are resuscitated with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES).Methods
BALB/c mice were randomly divided into control, NS, HTS, and HES resuscitation groups. Three subgroups (n=8) in each resuscitation group were marked as 2, 24, and 72 h. Flow cytometry was used to detect the MDSCs, monocytic MDSCs (M-MDSCs), and granulocytic/neutrophilic MDSCs (G-MDSCs) in peripheral blood nucleated cells (PBNCs), spleen single-cell suspension, and bone marrow nucleated cells (BMNCs).Results
The MDSCs in BMNCs among three resuscitation groups were lower 2 h after shock, in PBNCs of the HTS group were higher, and in spleen of the NS group were lower (all P<0.05 vs. control). The M-MDSC/G-MDSC ratios in PBNCs of the HTS and HES groups were lower (both P<0.05 vs. control). At 24 h, the MDSCs in PBNCs of the NS and HTS groups were higher, while the spleen MDSCs in the HTS group were higher (all P<0.05 vs. control). The M-MDSC/G-MDSC ratios were all less in PBNCs, spleen, and BMNCs of the NS and HTS groups, and were lower in BMNCs of the HES group (all P<0.05 vs. control). At 72 h, the elevated MDSCs in PBNCs were presented in the HTS and HES groups, and in spleen the augment turned up in three resuscitation groups (all P<0.05 vs. control). The inclined ratios to M-MDSC were exhibited in spleen of the NS and HTS groups, and in PBNCs of the NS group; the inclination to G-MDSC in BMNCs was shown in the HES group (all P<0.05 vs. control).Conclusions
HTS induces the earlier elevation of MDSCs in peripheral blood and spleen, and influences its distribution and differentiation, while HES has a less effect on the distribution but a stronger impact on the differentiation of MDSCs, especially in bone marrow.14.
为探讨肠淋巴液对肠系膜上动脉闭塞性休克(SMAO休克)的影响,对SMAO休克大鼠给予肠淋巴液或血浆治疗,并以生理盐水作对照,观察其对血压及存活率的影响。结果表明,开夹后90min起,肠淋巴液治疗组的血压显著高于对照组(P<0.05),其4h存活率也显著优于对照组(P<0.05),而血浆组与对照组血压和存活率未见统计学差异(P>0.05),提示肠淋巴液对SMAO休克具有提升血压及延长存活时间的作用。 相似文献
15.
Betul Apaydin Yildirim Saban Kordali Kubra Asena Terim Kapakin Fatih Yildirim Esra Aktas Senocak Serdar Altun 《Journal of Zhejiang University. Science. B》2017,18(6):501-511
The aim of this study was to evaluate the possible therapeutic or protective effects of Helichrysum plicatum DC. subsp. plicatum ethanol extract (HPE) against gentamicin-induced nephrotoxicity. Thirty-six Sprague Dawley male rats weighing between 200 and 250 g were used as live material. They were formed into six groups containing 6 rats each and were allowed to adapt to laboratory conditions for 7 d. Group I: control, 5% DMSO intraperitoneal (i.p.); Group II: HPE 100 mg/(kg·d) i.p.; Group III: HPE 200 mg/(kg·d) i.p.; Group IV: gentamicin as 80 mg/(kg·d) i.p.; Group V: gentamicin as 80 mg/(kg·d) i.p.+HPE 100 mg/(kg·d) i.p.; and Group VI: gentamicin as 80 mg/(kg·d) i.p.+HPE 200 mg/(kg·d) i.p. for 8 d. Following treatment, serum, liver, and kidney tissues were used to assess blood urea nitrogen (BUN), creatinine, enzymatic and non-enzymatic antioxidants, and lipid peroxidation. Gentamicin significantly increased serum BUN, creatinin, and liver and kidney levels of malondialdehyde (MDA). It also decreased the activity of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD). Treatment with the HPE 100 mg/kg reversed gentamicin-induced alterations as evidenced by decreased serum BUN and creatinin, liver and kidney oxidant marker, and tubular necrosis as well as by an increase in antioxidant enzymes. It was found that HPE 200 mg/kg significantly increased liver and kidney tissue MDA levels in nephrotoxicity in rats. As a result, these findings support the proposition that HPE in 100 mg/kg dose demonstrates in the kidney and liver as free radicals and scavenger to prevent the toxic effects of gentamicin in both the biochemical and histopathology parameters. 相似文献
16.
[目的]探讨大鼠脑出血双侧纹状体区细胞凋亡的时程变化规律和早期立体定向血肿抽吸术干预治疗对细胞凋亡的影响.[方法]雄性Sprague-Dawley(SD)大鼠80只,随机分成正常对照组,假手术组,脑出血自然恢复组,6 h血肿抽吸组,12 h血肿抽吸组以及24 h血肿抽吸组.采用立体定向注入Ⅶ型胶原酶建立脑出血模型.采用原位末端标记法检测出血周边组织细胞的凋亡.[结果]①大鼠脑出血周边组织12 h出现凋亡细胞,3d凋亡细胞达峰值,11 d仍有凋亡细胞表达.②血肿抽吸术干预后出血周边组织凋亡细胞数与脑出血组对应时间点比较显著下降(P<0.05).③6 h血肿抽吸组与24 h血肿抽吸组比较细胞凋亡数明显低于24 h血肿抽吸组,差异有显著性(P<0.05).[结论]立体定向血肿抽吸术干预治疗大鼠脑出血可明显影响细胞凋亡的表达,脑出血各种临床治疗的“时间窗”应为出血后12 h之内.早期(6 h)立体定向血肿抽吸术治疗可以显著减少脑出血后细胞凋亡. 相似文献
17.
淋巴液对正常血压大鼠的平均动脉压[MAP]、左室收缩压[LVSP]及左室舒张末压[LVEDP]与生理盐水对照组各项指标相比无显著差异[P>>0.05];而对失血性休克大鼠的MAP、LVSP具有显著回升作用[P<0.01],而对休克时LVEP的恢复无明显作用[P>0.05]。提示:淋巴液只能提高休克心脏的收缩功能,在低血容量情况下对休克心脏的舒张功能无明显影响,对正常心脏的收缩和舒张功能均无明显影响。 相似文献
18.
背景:氧化应激形成诱导多细胞通路的基础,其能导致糖尿病并发症,最能导致身体虚弱的疾病——是神经系统疾病. 研究的目的是评价跑台运动是否能减轻链脲霉素诱导的糖尿病大鼠海马氧化应激和细胞凋亡率.方法:40只雄性Wistar大鼠随机分为4组,每组10只,即对照组、运动组、糖尿病组和糖尿病运动组,通过给大鼠注射链脲霉素诱导糖尿病模型. 所有运动组大鼠在动物电动跑台上进行8周运动,在8周末,大鼠海马在冰冻中立即分离并冷冻保存. 收集上清液于-80°C保存,用于测定抗氧化酶和TBARs,用ELISA试剂盒检测细胞死亡检测细胞凋亡指数. 结果:糖尿病不运动组和糖尿病运动组的TBARs水平显著高于对照组;运动组SOD和GPx显著增高,糖尿病不运动组显著降低;与对照组相比,糖尿病不运动组CAT活性显著降低;与对照组相比,糖尿病不运动组细胞凋亡率显著增加,运动组显著降低. 结论:运动对糖尿病大鼠产生有益影响,部分原因可能是因为运动诱导氧化应激适应能力的改变. 相似文献
19.
INTRODUCTION In 1998 gastrointestinal pathologists reached aconsensus on the definition of chronic atrophic gas- tritis (CAG), which was described as programmed loss of gastric gland and/or replacement by intestinal glands in gastric mucosa. CAG was recognized to be closely related with development of gastric cancer and listed as precancerous lesions in this meeting (Genta, 1998). According to Correa (1992)’s cascade of gas-tric carcinogenesis, gastric cancer was believed to develop fr… 相似文献
20.
20只大鼠分为2组做瓯柑橘皮体内抗氧化作用试验。对照组10只饲喂基础饲料,试验组10只在基础饲料中添加10%的瓯柑橘皮,试验期2周。结果：试验组大鼠血清、心肌、肝脏、肾脏的SOD比对照组升高176.5%、39.0%、43.1%、49.5%;血清、心肌、骨骼肌、肝脏、肾脏的GSH-Px比对照组升高27.9%、122%、99.4%、62.3%、90.7%（均P〈0.05或0.01）;而血清、心肌、肾脏的MDA比对照组降低29.9%、48.7%和20.5%（P〈0.05和0.01）。结果证明瓯柑橘皮有显著的体内抗氧化作用。 相似文献