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1.
Hypertonic saline resuscitation reduces apoptosis of intestinal mucosa in a rat model of hemorrhagic shock 总被引：1，自引：0，他引：1
Objective: To investigate the early effects of hypertonic and isotonic saline solutions on apoptosis of intestinal mueosa in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 21 Spragne-Dawley (SD) rats. The rats were randomly divided into the sham group, normal saline resuscitation (NS) group, and hypertonic saline resuscitation (HTS) group, with 7 in each group. We detected and compared the apoptosis in small intestinal mucosa of rats after hemorrhagic shock and resuscitation by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), FITC (fluo-rescein-iso-tbiocyanate)-Annexin V/PI (propidium iodide) double staining method, and flow cytometry. Results: In the early stage of hemorrhagic shock and resuscitation, marked apoptosis of small intestinal mucosa in the rats of both NS and HTS groups was observed. The numbers of apoptotic cells in these two groups were significantly greater than that in the sham group (P＜0.01). In the HTS group, the apoptic cells significantly decreased, compared with the NS group (P＜0.01). Conclusion: In this rat model of severe hemorrhagic shock, the HTS resuscitation of small volume is more effective than the NS resuscitation in reducing apoptosis of intestinal mucosa in rats, which may improve the prognosis of trauma. 相似文献
2.
Feng Yao Yuan-qiang Lu Jiu-kun Jiang Lin-hui Gu Han-zhou Mou 《Journal of Zhejiang University. Science. B》2017,18(5):402-409
Objective
To investigate the effects of resuscitation with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES) on regulatory T cells (Tregs), helper T 1 (Th1)/Th2 and cytotoxic T 1 (Tc1)/Tc2 profiles in the treatment of hemorrhagic shock.Methods
Rats subjected to severe hemorrhagic shock were resuscitated for 30 min with NS (n=8), HTS (n=8), or HES (n=8); sham (n=8) and naive control (n=8) groups were used for comparison. Following fluid resuscitation, the whole shed blood was reinfused for 30 min, and the rats were observed with continuous hemodynamic monitoring for 120 min. CD4+CD25+Foxp3+ Treg proportions, Th1/Th2 and Tc1/Tc2 profiles in spleen were analyzed by three-color flow cytometry.Results
The proportion of CD4+CD25+Foxp3+ Tregs and ratios of Th1/Th2 and Tc1/Tc2 did not differ among control, sham, and HTS groups, but were significantly lower in NS and HES groups (both P<0.05 vs. sham); NS and HES levels were similar. The level of Tc1 was significantly increased in HTS (P<0.05 vs. sham), and levels of Tc2 were increased in NS, HES, and HTS groups compared to sham (all P<0.05), but did not differ from each other.Conclusions
HTS resuscitation has a greater impact on immune system recovery than NS or HES by preserving the proportion of Tregs and maintaining the balance between Th1/Th2 and Tc1/Tc2 cells in the spleen. Thus, HTS resuscitation provides potential immunomodulatory activity in the early stage after hemorrhagic shock.3.
Jiu-kun Jiang Wen Fang Liang-jie Hong Yuan-qiang Lu 《Journal of Zhejiang University. Science. B》2017,18(1):48-58
Objective
To investigate the distribution and differentiation of myeloid-derived suppressor cells (MDSCs) in hemorrhagic shock mice, which are resuscitated with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES).Methods
BALB/c mice were randomly divided into control, NS, HTS, and HES resuscitation groups. Three subgroups (n=8) in each resuscitation group were marked as 2, 24, and 72 h. Flow cytometry was used to detect the MDSCs, monocytic MDSCs (M-MDSCs), and granulocytic/neutrophilic MDSCs (G-MDSCs) in peripheral blood nucleated cells (PBNCs), spleen single-cell suspension, and bone marrow nucleated cells (BMNCs).Results
The MDSCs in BMNCs among three resuscitation groups were lower 2 h after shock, in PBNCs of the HTS group were higher, and in spleen of the NS group were lower (all P<0.05 vs. control). The M-MDSC/G-MDSC ratios in PBNCs of the HTS and HES groups were lower (both P<0.05 vs. control). At 24 h, the MDSCs in PBNCs of the NS and HTS groups were higher, while the spleen MDSCs in the HTS group were higher (all P<0.05 vs. control). The M-MDSC/G-MDSC ratios were all less in PBNCs, spleen, and BMNCs of the NS and HTS groups, and were lower in BMNCs of the HES group (all P<0.05 vs. control). At 72 h, the elevated MDSCs in PBNCs were presented in the HTS and HES groups, and in spleen the augment turned up in three resuscitation groups (all P<0.05 vs. control). The inclined ratios to M-MDSC were exhibited in spleen of the NS and HTS groups, and in PBNCs of the NS group; the inclination to G-MDSC in BMNCs was shown in the HES group (all P<0.05 vs. control).Conclusions
HTS induces the earlier elevation of MDSCs in peripheral blood and spleen, and influences its distribution and differentiation, while HES has a less effect on the distribution but a stronger impact on the differentiation of MDSCs, especially in bone marrow.4.
Lu Yuan-qiang Cai Xiu-jun Gu Lin-hui Fan Yu-jing Wang Qi Bao De-guo 《浙江大学学报(A卷英文版)》2005,6(9):907-912
Objective: To observe the effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic
shock. Methods: A model of rat with severe hemorrhagic shock and active bleeding was established in 32 SD (Sprague-Dawley)
rats. The rats were randomly divided into control group, no fluid resuscitation group (NF group), controlled fluid resuscitation
group (NS40 group) and rapid large scale fluid resuscitation group (NS80 group). Each group contained 8 rats. The curative
effects were compared. At the same time, the apoptosis in liver, kidney, lung and small intestinal mucosa of survivors after
hemorrhage and resuscitation was detected by light microscopy in HE (hematoxylin and eosin) stained tissue sections, flow
cytometry and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Results: The survival rate of early fluid
resuscitation (14/16) was markedly higher than that of NF group (3/8). There was some apoptosis in liver, kidney, lung and
small intestinal mucosa of all survivors. Compared with NF and NS40 groups, the apoptosis of liver, kidney and small intestinal
mucosa of NS80 group was obviously increased. Conclusions: Among three fluid resuscitation methods, controlled fluid resuscitation
can obviously improve the early survival rate and the apoptosis of liver, kidney and small intestinal mucosa in rats with
severe and uncontrolled hemorrhagic shock, and may benefit improvement of prognosis. 相似文献
5.
Lu YQ Cai XJ Gu LH Fan YJ Wang Q Bao DG 《Journal of Zhejiang University. Science. B》2005,6(9):907-912
INTRODUCTION Hemorrhagic shock is a common clinic emer- gency case. Successful treatment includes surgical control of hemorrhage and restoration of tissue per- fusion. Current guidelines for presurgical treatment of patients with hemorrhagic shock recommend rapid volume resuscitation to normal blood pressure as quickly as possible. The practice is controversial because aggressive restoration of intravascular vol- ume and rapid increasing of blood pressure before securing hemostasis may ex… 相似文献
6.
目的:探讨川芎嗪、当归注射液对失血性休克大鼠血液动力学的影响。方法:颈动脉放血复制大鼠失血性休克模型,通过微循环观察、器官血流和血液流变学测定方法,观察川芎嗪、当归注射液对失血性休克大鼠血液动力学的影响。结果:失血性休克时,微循环明显障碍,主要表现为微血流变慢和血细胞聚集;经川芎嗪、当归注射液治疗后,微循环改善,血粘度、血小板粘附率和聚集率降低,器官微区血流量增加,与NS组比较有显著性差异(P<0.05)。结论:川芎嗪、当归注射液能明显改善失血性休克大鼠微循环障碍,降低血小板聚集率,增加器官血液灌流量。 相似文献
7.
目的：为进一步探索ＬＡＮ细胞疫苗在ＡＡ大鼠治疗中的作用．方法：近交系ＳＤ 大鼠以完全弗氏佐剂致炎形成佐剂关节炎（adjuvant arthritis,AA）．分离ＡＡ大鼠淋巴细 胞,经培养扩增后,采用已建立的肿瘤疫苗专利技术制成ＬＡＫ细胞疫苗．实验动物分别 于腋下注射ＬＡＫ细胞疫苗,每５日一次共３次．治疗前及治疗后一周分别采外周血测定 Ｔ细胞亚群,同时行外周血涂片和骨髓细胞学检查．结果：经ＬＡＫ细胞疫苗免疫后,ＡＡ 实验动物组外周血淋巴细胞百分率较对照组降低（Ｐ＜０.０１）,Ｔ细胞亚群ＣＤ８＋较对照组明 显上升（Ｐ＜０．０５）,ＣＤ４／ＣＤ８比值下降,两组差别具有显著性意义（Ｐ＜０．０５）。结论： ＬＡＫ细胞疫苗可有效地降低佐剂关节炎大鼠外周血淋巴细胞的数目并提高ＣＤ８＋Ｔ细胞 亚群的数量,在治疗自身免疫性疾病中可能是一种有用的手段,值得进一步研究． 相似文献
8.
目的通过肺组织NF-κB的表达,研究羟乙基淀粉130/0.4复苏对失血性休克大鼠肺损伤保护作用的可能机制.方法健康雄性SD大鼠54只,体重230~280 g,随机分为3组：对照组（C组）、乳酸林格氏液复苏组（RL组）、6%羟乙基淀粉130/0.4复苏组（HES组）,按照Wiggers改良法复制失血性休克模型.于复苏后1 h、2 h4、h采用免疫组织化学的方法检测肺组织NF-κB的表达;光镜下观察肺组织形态学改变.结果 RL组与HES组肺组织NF-κB免疫组化染色细胞核阳性百分率较C组明显升高,RL组升高的程度较HES组明显（P〈0.01）.免疫组化染色光镜观察显示：HES组比RL组的组织损伤明显减轻.结论 6%羟乙基淀粉130/0.4复苏失血性休克可以抑制肺组织NF-κB活性,减轻休克及复苏过程造成的肺损伤. 相似文献
9.
目的:对失血性休克患者应用高渗氯化钠羟乙基淀粉40注射液治疗,观察临床疗效,并对该药物在应用中需注意的事项进行分析总结。方法:2005.06.01~2006.12.01间所收治的143例失血性休克患者,83例使用高渗氯化钠羟乙基淀粉40注射液治疗,60例使用传统药物治疗,并观察其神志、皮肤色泽及温度、呼吸、脉搏、血压、尿量变化,并进行分析对比,对药物应用中出现的情况或现象进行分析,来对比所用药物的作用及效果,随后辅以其他药物及方法治疗。结果:患者在用高渗氯化钠羟乙基淀粉40注射液后,神志较短时间内(5~20min)明显改善,皮肤色泽及温度趋于正常,呼吸次数减少并趋于正常,增高的脉搏次数明显下降,收缩压及舒张压有一定程度的的升高(收缩压较显著),尿量有所增加,神志明显改善;从临床症状指标上比传统药物起效快而且疗效明确(P<0.05)。结论:高渗氯化钠羟乙基淀粉40注射液在失血性休克患者早期应用效果明显,能够迅速提升血压,改善病人的休克状态,赢得抢救的时间,继而配合使用其他治疗,能提高体克病人的抢救成功率。 相似文献
10.
本实验采用肠淋巴管引流术,在失血性休克大鼠模型的基础上,观察高渗盐水治疗失血性休克过程中肠淋巴流量及其蛋白含量的变化,旨在探讨高渗盐水对失血性休克大鼠治疗的淋巴机制。结果表明,休克大鼠输入高渗盐水或生理盐水后,两组大鼠的血压、肠淋巴流量及其蛋白输出量均比休克期明显升高,高渗盐水治疗组显著高于生理盐水对照组(P<0.01),而且治疗组大鼠肠淋巴流量及其蛋白输出量远远高于休克前水平(P<0.01)。结果提示,高渗盐水的抗休克机制可能与其恢复休克大鼠的淋巴流量及其蛋白输出量、改善休克时肠淋巴循环障碍有关。 相似文献
11.
为探讨肠淋巴液对肠系膜上动脉闭塞性休克(SMAO休克)的影响,对SMAO休克大鼠给予肠淋巴液或血浆治疗,并以生理盐水作对照,观察其对血压及存活率的影响。结果表明,开夹后90min起,肠淋巴液治疗组的血压显著高于对照组(P<0.05),其4h存活率也显著优于对照组(P<0.05),而血浆组与对照组血压和存活率未见统计学差异(P>0.05),提示肠淋巴液对SMAO休克具有提升血压及延长存活时间的作用。 相似文献
12.
目的：探讨腹腔镜与开腹手术治疗卵巢良性畸胎瘤患者对其机体免疫功能造成的影响。方法：研究组卵巢良性畸胎瘤患者给予腹腔镜手术剥除治疗;对照组卵巢良性畸胎瘤患者给予传统开腹手术剥除治疗。观察并记录两组患者治疗前后机体中IL-6（白细胞介素）与T淋巴细胞亚群（CD4／CD8）的含量,并进行统计学分析。结果：研究组与对照组患者治疗前IL-6、CD4／CD8检测结果无明显差异,且P〉0．05,两组患者对比结果无统计学意义;研究组与对照组患者手术后1d与手术后4d IL-6、CD4／CD8检测结果有明显差异,且研究组患者术后4d基本恢复术前水平,P〈0．05,两组患者对比结果有统计学意义。结论：使用腹腔镜治疗卵巢良性畸胎瘤对患者造成的机体免疫功能影响较传统开腹手术小,有利于患者术后恢复机体健康以及预防各类并发症,值得临床推广应用。 相似文献
13.
[目的]通过对免疫球蛋白(IgA、IgG、IgM),补体(C3、C4),T淋巴细胞亚群(CD3、CD4、CD8),可溶性白细胞介素2受体(SIL-2R)的检测,研究这些免疫指标与NIDDM(非胰岛素依赖性糖尿病)合并肺部感染患者的关系.[方法]采用双抗夹心法测SIL-2R,速率散射比浊法测免疫球蛋白和补体,单克隆法测淋巴细胞亚群等免疫指标.[结果]①糖尿病及其合并感染组SIL-2R显著高于正常对照组(P<0.01).CD4/CD8显著低于正常对照组(P<0.05);②合并感染组较正常对照组IgG、IgA、C3、C4显著升高(P<0.05、P<0.01、P<0.05、P<0.05),IgM无显著变化(P<0.05).[结论]NIDDM合并肺部感染可加重糖尿病及机体免疫功能低下,NIDDM合并肺部感染T淋巴细胞亚群(CD3、CD4、CD8),补体(C3、C4)免疫球蛋白(IgA、IgG)可溶性白细胞介素2受体(SIL-2R)的检测,可作为糖尿病及其合并感染者的病情变化的客观指标. 相似文献
14.
Objective: To investigate the cytokine response pattern (IL-4/IFN-γ) in infants with RSV infections and bronchiolitis during the acute phase. Methods: Four-color flow cytometry was used to measure intracellular IL-4 and IFN-γ expressions in peripheral blood CD3+ and CD8+ lymphocytes from RSV-infected and bronchiolitis infants. Serum IL-4 and IFN-γ levels were also determined. Results: RSV-infected and bronchiolitis infants showed no statistical differences from not-RSV-infected or pneumonia infants and control in the frequency of IL-4 and IFN-γ expressions in CD3+CD8- lymphocytes, showed no obvious Th1/Th2 imbalance, while IFN-γ was expressed much more frequently in CD3+CD8+ lymphocytes. Systematically, RSV-infected and bronchiolitis infants showed much lower levels of serum IL-4 and IL-4/IFN-γ ratios and much higher serum IFN-γ levels than control. However, there were no statistical differences in the above three indices between RSV-infected and not-RSV infected infants or between bronchiolitis and pneumonia infants, except that bronchiolitis infants had a higher level of serum IFN-γ than pneumonia infants statistically. Conclusions: There is no type-2 cytokine response predominance in the acute phase of RSV infection and bronchiolitis. IL-4 production is suppressed and IFN-γ production upregulated, the latter being most prominent in bronchiolitis infants. 相似文献
15.
淋巴液对正常血压大鼠的平均动脉压[MAP]、左室收缩压[LVSP]及左室舒张末压[LVEDP]与生理盐水对照组各项指标相比无显著差异[P>>0.05];而对失血性休克大鼠的MAP、LVSP具有显著回升作用[P<0.01],而对休克时LVEP的恢复无明显作用[P>0.05]。提示:淋巴液只能提高休克心脏的收缩功能,在低血容量情况下对休克心脏的舒张功能无明显影响,对正常心脏的收缩和舒张功能均无明显影响。 相似文献
16.
目的:观察一氧化氮(NO)供体硝酸异山梨酯(Isosorbride Dinitrate,ID)及一氧化氮合酶(NOS)抑制剂氨基胍(Aminoguanidine,AG)对双侧卵巢切除(OVX)大鼠骨组织形态计量学的影响。方法:49只雌性大鼠随机分成7组,每组7只。①假手术(Sham)组、②卵巢切除(OVX)组、③卵巢切除 雌激素(OVX EB)组、④卵巢切除 NO供体(OVX ID)组、⑤卵巢切除 NO供体和雌激素(OVX ID EB)组、⑥卵巢切除 NOS抑制剂(OVX AG)组、⑦卵巢切除 NOS抑制剂和雌激素(OVX AG EB)组。实验10周后处死大鼠,对第4、5腰椎进行骨组织形态计量学分析。结果:与Sham组相比:OVX组、OVX AG组和OVX AG EB组组织形态计量学参数BV/TV和Tb.Th减少,Tb.Sp和MAR增加(P<0.05);而OVX EB组、OVX ID组和OVX ID EB组骨组织形态计量学参数BV/TV、Tb.Th、Tb.Sp和MAR均无明显差异(P>0.05)。与OVX组相比:OVX EB组、OVX ID组和OVX ID EB组骨组织形态计量学参数BV/TV和Tb.Th明显增加,Tb.Sp和MAR明显减少(P<0.05);而OVX AG组和OVX AG EB组骨组织形态计量学参数BV/TV、Tb.Th、Tb.Sp和MAR均无明显差异(P>0.05)。结论:NO供体预防OVX鼠骨丢失,NOS抑制剂则阻止雌激素预防OVX鼠骨丢失的作用。 相似文献
17.
18.
以少量胸导管淋巴液和肠淋巴液给予补液干予治疗的重症失血性休克大鼠,用生理盐水做对照,观察其对血压及存活时间的影响,以探讨不同部位淋巴液维持血压的共同特性。结果表明:胸导管淋巴液及肠淋巴液回升血压及延长存活时间作用均显著优于对照组(P<0.05～0.01),而两个治疗组间未见统计学差异(P>0.05)。结果提示:胸导管淋巴液和肠淋巴液对失血性休克大鼠均有较好的抗休克作用。 相似文献
19.
目的为探索RA生物治疗的新途径,以及进一步认识RA的发病机制方法：近交系SD大鼠以完全弗氏佐剂致炎形成佐剂关节炎（adjuvantarthritis,AA）．分离AA大鼠淋巴细胞,经培养扩增后,采用已建立的肿瘤疫苗专利技术制成LAK疫苗．实验动物分别于腋下注射LAK细胞疫苗,每周一次共2次．治疗前后分别采血测定WBC和RBC并作比较分析结果经LAK细胞疫苗免疫后,实验动物组外周血淋巴细胞数目较治疗前显著减少（23.75±9．36对10.82±6,38,p＜0.01）．同时实验动物组的贫血状况明显好转,RBC数量明显增加（6.40±1.67对9.24±2．21,P＜0．05）结论LAK细胞疫苗可降低佐剂关节炎外周血淋巴细胞的数目并改善其贫血状态,在治疗自身免疫性疾病中可能是一种有用的手段,值得进一步研究． 相似文献
20.
目的:检测子宫内膜异位症患者手术前后外周血T淋巴细胞亚群变化,了解病人细胞免疫功能状况。方法:采用间接免疫荧光法检测子宫内膜异位症患者体内CD3~ 、CD4~ 、CD8~ 细胞水平,并取同龄正常妇女作为对照。结果:(1)手术前与正常妇女相比,子宫内膜异位症患者体内存在CD8~ 细胞比例升高,CD4~ 细胞比例和CD4~ /CD8~ 比值下降;(2)手术后病人CD8~ 细胞逐渐回降,而CD4~ 细胞和CD4~ /CD8~ 比值呈现回升趋势。结论:子宫内膜异位症患者存在T细胞免疫异常,这种异常可在手术治疗后得以恢复。 相似文献