不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制研究     

李洁  杨静  赵丽丽 《武汉体育学院学报》2019,53(5):88-94

目的：探讨不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制。方法：将雄性Wistar大鼠50只随机均分为5组：安静对照组（C）、低负荷运动训练组（LT）、中等负荷运动训练组（MT）、高负荷运动训练组（HT）和极高负荷运动训练组（ST），每组10只。各运动组分别进行6周的跑台运动训练。训练方案结束后，取腓肠肌样本，提取线粒体，测定线粒体柠檬酸合成酶（CS）、异柠檬酸脱氢酶（ICD）和α-酮戊二酸脱氢酶（α-KGDHC）活性；线粒体Ga²⁺含量、胞浆NADH、NAD⁺、ATP和ADP含量，以及ICD mRNA转录水平。结果：（1）不同负荷运动训练组线粒体CS、ICD和α-KGDHC的活性均显著高于安静对照组（P < 0.01），且CS和ICD活性由高到低顺序均为：MT组 > HT组 > ST组 > LT组 > C组，α-KGDHC活性由高到低顺序为：HT组 > MT组 > ST组 > LT组 > C组。（2）不同负荷运动训练组线粒体Ca²⁺ 含量均显著高于安静对照组（P < 0.01），其含量由高到低顺序为：MT组 > HT组 > ST组 > LT组 > C组；胞浆NADH/NAD⁺和ATP/ADP的比值均显著低于安静对照组（P < 0.01），其比值由低到高顺序为：MT组 < ST组 < HT组 < LT组 < C组。（3）不同负荷运动训练组ICD mRNA转录水平均高于安静对照组（P < 0.01），其水平由高到低顺序为： MT组 > HT组 > ST组 > LT组 > C组。结论：低负荷、中等负荷、高负荷及极高负荷运动训练均可提高大鼠骨骼肌线粒体三羧酸循环功能，且中等负荷运动训练效果最佳。其机制与胞浆NADH/NAD⁺和ATP/ADP比值、线粒体摄钙能力及限速酶基因的表达有关。  相似文献   

短跑专项力量训练手段训练效应的实证研究——以拖重物跑为例     

王蓓  谢慧松  刘少飞 《武汉体育学院学报》2019,53(8):82-88

研究目的：探究拖重物跑训练手段对短跑运动员的身体素质、途中跑阶段的技术特征及下肢环节肌肉力量的影响，深入认识其对短跑途中跑技术和专项力量的作用机制。研究方法：对14名男子二级左右水平短跑运动员进行为期8周每周3次6%~10% BM负荷的拖重物跑训练，采用高速摄像分析法、等动肌力测试法分析运动员身体素质；支撑阶段髋、膝、踝关节运动学参数和下肢各环节肌肉力量实验前后的变化情况。结果：（1）实验后运动员30m、60m、立定跳远、立定三级跳远等身体素质及专项成绩显著提高；（2）步长、重心水平速度显著性提高，两大腿剪绞平均速度提高、单步时间减少；支撑阶段最小膝、踝角显著降低，角速度提高；（3）髋、膝关节伸/屈肌群PT/BW、AP除膝关节60°/sPT/BW值未见统计学意义，其余各角速度下PT/BW、AP均呈显著性差异，踝关节跖屈肌群各角速度下PT/BW值显著提高，跖屈肌群60°/s速度下AP提高，背屈肌群AP略降低。结论：适宜负荷的拖重物跑训练可显著改善短跑运动员运动素质、提高运动成绩；有利于提高髋关节剪绞-制动力量，使膝、踝关节处于低位超等长"屈蹬"状态；提高了髋、膝关节屈伸肌群快速主动收缩能力及踝关节跖屈肌群退让性快速收缩能力。  相似文献   

Reference power values for the jump squat exercise in elite athletes: A multicenter study     

Pedro L. Valenzuela  Michael McGuigan  Guillermo Sánchez-Martínez  Elaia Torrontegi  Javier Vázquez-Carrión  Zigor Montalvo 《Journal of sports sciences》2020,38(19):2273-2278

ABSTRACT

The present study aimed to provide reference values for lower-limb muscle power assessed during the incremental jump squat (JS) test in elite athletes (i.e., professional athletes competing at international level). We pooled data from all JS tests performed by elite athletes of different sports in two high-performance centres between 2015 and 2019, and computed reference values (i.e., terciles) for mean power (MP), mean propulsive power (MPP), and peak power (PP). Reference values were obtained from 684 elite athletes (458 male and 226 female) of 16 different sports (boxing, judo, karate, fencing, taekwondo, wrestling, basketball, soccer, futsal, handball, rugby union, badminton, tennis, long distance running, triathlon, and sprinting). Significant differences (p < 0.001) were found between male and female athletes for MP (7.47 ± 1.93 and 6.15 ± 1.68 W·Kg^?1, respectively), MPP (10.50 ± 2.75 and 8.63 ± 2.43 W·Kg^?1), and PP (23.64 ± 6.12 and 19.35 ± 5.49 W·Kg^?1). However, the velocity at which these power measures was attained seemed to be independent of sex (~0.95, 1.00 and 2.00 m·s^?1 for mean, mean propulsive, and peak velocity, respectively) and homogeneous across different sport disciplines (coefficient of variation <10%). These data can be used to classify athletes’ power capabilities, and the optimum velocity ranges provided here could be useful for training purposes.  相似文献   

Combined effects of exposure to hypoxia and cool on walking economy and muscle oxygenation profiles at tibialis anterior     

Masahiro Horiuchi  Yoko Handa-Kirihara  Daijiro Abe  Yoshiyuki Fukuoka 《Journal of sports sciences》2019,37(14):1638-1647

We investigated combined effects of ambient temperature (23°C or 13°C) and fraction of inspired oxygen (21%O₂ or 13%O₂) on energy cost of walking (C_w: J·kg^?1·km^?1) and economical speed (ES). Eighteen healthy young adults (11 males, seven females) walked at seven speeds from 0.67 to 1.67 m s^?1 (four min per stage). Environmental conditions were set; thermoneutral (N: 23°C) with normoxia (N: 21%O₂) = NN; 23°C (N) with hypoxia (H: 13%O₂) = NH; cool (C: 13°C) with 21%O₂ (N) = CN, and 13°C (C) with 13%O₂ (H) = CH. Muscle deoxygenation (HHb) and tissue O₂ saturation (StO₂) were measured at tibialis anterior. We found a significantly slower ES in NH (1.289 ± 0.091 m s^?1) and CH (1.275 ± 0.099 m s^?1) than in NN (1.334 ± 0.112 m s^?1) and CN (1.332 ± 0.104 m s^?1). Changes in HHb and StO₂ were related to the ES. These results suggested that the combined effects (exposure to hypoxia and cool) is nearly equal to exposure to hypoxia and cool individually. Specifically, acute moderate hypoxia slowed the ES by approx. 4%, but acute cool environment did not affect the ES. Further, HHb and StO₂ may partly account for an individual ES.  相似文献   

Effects of periodic carbohydrate ingestion on endurance and cognitive performances during a 40-km cycling time-trial under normobaric hypoxia in well-trained triathletes     

Yi-Hung Liao  Toby Mündel  Yan-Ting Yang  Chen-Chan Wei  Shiow-Chwen Tsai 《Journal of sports sciences》2019,37(16):1805-1815

The purpose of this study was to examine CHO ingestion on a cognitive task using a field-simulated time-trial (TT) under hypoxia in well-trained triathletes. Ten male triathletes (age: 22.1 ± 1.1 years; VO₂max: 59.4 ± 1.4 ml/kg/min) participated in this double-blind/crossover/counter-balanced design study. Participants completed 3 TT trials: 1) normoxic placebo (NPLA; FiO₂ = 20.9%), 2) hypoxic placebo (HPLA; FiO₂ = 16.3%), and 3) hypoxic CHO (HCHO; 6% CHO provided as 2 ml/kg/15 min; FiO₂ = 16.3%). During the TT, physiological responses (SpO₂, HR, RPE, and blood glucose/lactate), cognitive performance, and cerebral haemodynamics were measured. Hypoxia reduced TT performance by ~3.5–4% (p < 0.05), but CHO did not affect TT performance under hypoxia. For the cognitive task, CHO slightly preserved exercise-induced cognitive reaction speed but did not affect response accuracy during hypoxic exercise. However, CHO did not preserve the decreased Hb-Diff (cerebral blood flow, CBF) and increased HHb in the prefrontal lobe (p < 0.05) during hypoxic exercise, and CHO failed to preserve hypoxia-suppressed prefrontal CBF and tissue oxygen saturation. In conclusion, the present study demonstrates that CHO is effective in sustaining reaction speed for a cognitive task but not promoting TT performance during hypoxic exercise, which would be important for strategy-/decision-making when athletes compete at moderate high-altitude.  相似文献   

Physiological responses in different intensities of resistance exercise – Critical load and the effects of aging process     

Vivian Maria Arakelian  Cássia da Luz Goulart  Renata Gonçalves Mendes  Nuno Manoel de Sousa  Renata Trimer  Solange Guizilini 《Journal of sports sciences》2019,37(12):1420-1428

Aim was to identify critical load (CL) in young and elderly apparently healthy male cohorts. To contrast the metabolic, cardiovascular and perceptual responses on CL according to age. We evaluated 12 young (23 ± 3 years) and 10 elderly (70 ± 2 years) apparently healthy active males, who underwent: (1) 1 repetition maximum (1RM) test on a 45° Leg Press; (2) on different days, three high-intensity resistance exercise constant load tests (60%, 75% and 90% 1RM) until fatigue (Tlim). Absolute values of both the CL asymptote and curvature constant (kg) were significantly lower in elderly subjects (P < 0.05). In contrast, elderly subjects demonstrated a significantly higher number of repetitions at CL when compared with young subjects (P < 0.05). As expected, oxygen uptake (VO₂) and heart rate (HR) during maximal aerobic exercise testing were significantly reduced in older subjects. However, percent-predicted aerobic capacity were higher in older subjects (P < 0.05). In addition, blood lactate ([La^?]) corrected to Tlim and rating of perceived exertion values were greater in younger subjects at all intensities (P < 0.05). These findings, despite reduced force production in older subjects, endurance-related parameters are well preserved according to age-adjusted percent-predicted values in apparently healthy males.  相似文献   

上肢康复机器人主动康复训练控制策略     

刘鹏  葛小青  李宇  赵佳欣 《中国科学院大学学报》2019,36(4):570-576

近年来，脑卒中和交通事故造成上肢损伤的人数逐年上升，医疗领域对康复器械的需求越来越大。为满足实际需求，开发以气动人工肌肉为驱动器的4自由度（肩关节外收/内展（被动）、前驱/后伸、肘关节屈曲、腕关节屈曲）可穿戴上肢康复机器人。首先设计上肢康复机器人的结构，并采用气动人工肌肉作为肘关节与腕关节的驱动器。然后针对该上肢康复机器人，研究主动康复训练控制策略，设计两种控制策略。通过穿戴实验验证控制策略的有效性和实际应用价值。  相似文献   

NiCo_2O_4纳米结构阵列的水热合成与氧析出特性研究     

陈传胜  张作胜  陈伟杰  乔浏 《安徽职业技术学院学报》2020,(1):16-19,41

钴酸镍纳米材料具有良好的电催化氧析出反应(Oxygen Evolution Reaction,OER)活性,是一种很有发展潜力的非贵金属电催化材料。文章以碳纤维纸为基底,采用水热合成的方法对纳米结构钴酸镍的生长进行了系统的研究,分别考察了生长温度、前驱体浓度和配比、生长时间对产物的形貌、尺寸、物相结构、OER电化学性能的影响,通过对比分析后发现在生长温度100℃、生长时间5h、镍钴比为2:1时所获得的多孔片状钴酸镍纳米结构具有最佳的OER特性。  相似文献   

A multi-nation examination of the fatigue and recovery time course during the inaugural Under-18 Six Nations rugby union competition     

Carlos Ramírez-López  Kevin Till  Thomas Sawczuk  Pierosario Giuliano  Alexis Peeters  Grant Beasley 《Journal of sports sciences》2020,38(6):644-651

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Protective Role of <Emphasis Type="SmallCaps">l</Emphasis>-Arginine Against Free-Radical Mediated Oxidative Damage in Patients with Unstable Angina     

Tripathi P  Chandra M  Misra MK 《Indian journal of clinical biochemistry : IJCB》2010,25(3):302-306

Unstable angina is a critical condition of heart resulting from narrowing of vessels supplying blood to heart. Ischemia of the myocardium leads to oxidative stress and severe tissue damage. The objective of the present study was to determine the effect of l-arginine administration on the oxidant–antioxidant homeostasis which otherwise gets imbalanced in patients with cardiovascular diseases. The results obtained, show improvement in the oxidant–antioxidant levels of the subjects upon incorporation of l-arginine. Our findings suggest that supplementation of l-arginine along with regular anti-anginal therapy may be beneficial to the patients of unstable angina.  相似文献