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碱性纤维素酶产生菌的筛选及其产酶条件 总被引：4，自引：0，他引：4
从土壤样品中筛选到一株产碱性纤维素酶的菌株ＳＹＨ－１,产酶的最适碳源为麸皮,最适氮源为豆饼粉,摇瓶发酵最高酶活２５ｕ／ｍＬ,酶反应的最适温度为５０℃,ｐＨ为９．６,主要作用底物为ＣＭＣ,对滤纸,纤维素粉和结晶纤维素几乎作用。 相似文献
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介绍了１５０株三元能量处理妥布拉霉素（ＴＯＢ）正突变株的筛选研究，通过初筛获得２５株ＴＢＯ突变株，从中分离出１５株高产菌，进一步复筛双从中挑选出５株产量更高的产高菌株。通过最佳培养基配方的发酵试验，确定联抗性能最佳的菌株为ＱＢ－１３０－Ｓ１。实验表明：三元能量用于ＴＯＢ菌诱变，不仅提高了产抗水平，而且菌株生长时间缩短，经４次传代，高产菌株的遗传性能稳定。 相似文献
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对黑曲霉HYA4菌株所产果胶酶的酶学性质进行了研究。结果表明：该酶反应最适温度为48℃,果胶酶最适pH值为4.8,果胶酶活力稳定的pH范围为3.0-6.0;Km值为4.33mg/mL;金属离子Ca2＋、Mg2＋对果胶酶有较强的激活作用,Ba2＋对果胶酶也有一定的激活作用,Ag＋、Mn2＋、Zn2＋、Fe3＋、K＋对果胶酶有抑制作用。由SDS-PAGE测定果胶酶的相对分子量为46000＆#177;3000Da;IEF电泳测定其等电点为4.7＆#177;0.2。 相似文献
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《科技风》2017,(17)
对一株产纤维素酶的黄曲霉进行发酵条件优化和酶学性质初步探究,利用DNS法测定CMC酶活、滤纸酶活,结果显示此菌株纤维素酶最佳反应温度为50℃,最佳反应pH为7.0。在金属离子中,发现Zn~(2+)具有抑制作用,最大抑制浓度为0.4 mg/m L,Mg~(2+)、Mn~(2+)、Ca~(2+)、Fe~(3+)、Fe~(2+)具有促进作用,最适浓度分别为0.4、1、1、0.8、1.4 mg/mL。蛋白胨和酵母粉具有促进作用,最适浓度分别为1.0%、0.6%,最适pH为6.0。综合优化后,测得滤纸酶活和CMC酶活分别为7.73 U/mL、11.28 U/mL,相比优化前提高了38.8%和43.7%。 相似文献
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浙江养殖鱼类暴发性流行病病原的研究——Ⅰ.嗜水气单胞菌(Aeromonas hydrophila)的分离、致病性及生理生化特性 总被引：2，自引：0，他引：2
从患病鲫、鲢、鳙、鳊等鱼体上分离出一菌株,经腹腔注射及浸泡感染,能引起鱼患典型的出血病症和死亡,其半数致死量(LD_(50))为1.48×10~5个菌/鱼.该菌发酵分解糖类,氧化酶和过氧化氢酶实验为阳性,具极生单鞭毛,革兰氏阴性,对弧菌抑制剂不敏感,经生理生化特性测定及形态、培养特征观察,确认为嗜水气单胞菌(Aermonas hydrophila).并测定了该菌对抗生素的敏感性及生长特性。 相似文献
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本文报道使用三株霍乱弧菌型特异性单克隆抗体(VC-McAb),以干菌快速酶标法对1088株历年分离保存的霍乱弧菌EL-Tor生物型菌株进行了血清型和流行菌型的分型鉴定,并与原有的血清学、噬菌体和生物分型比较,证明VC-McAb对菌株的分型率分别为小川型流行株90.42％,小川型非流行株97.04％,稻叶型流行株89.34％和稻叶型非流行株93.94％。本法快速(仅需2h)、简便、敏感、特异,对霍乱弧菌血清型和流行菌型的快速分型鉴定,具有较大的实用价值。 相似文献
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《科技通报》2016,(3)
从岱山盐田采集土壤样品,利用含不同Na Cl浓度的RM培养基分离中度嗜盐菌,并采用抑菌圈法进行抗菌活性筛选。结果共分离得到106株中度嗜盐细菌菌株,其中有27株显示出对革兰氏阳性菌、革兰氏阴性菌和人类病原真菌的有效抗性。通过基于16S r RNA基因序列的系统发育分析,具有抗菌活性的27株菌株分别属于7个属,即嗜盐单胞菌属(Halomonas)、喜盐芽孢杆菌属(Halobacillus)、海杆菌属(Marinobacter)、产微球茎菌属(Microbulbifer)、Piscibacillus属、芽孢杆菌属(Bacillus)和枝芽孢杆菌属(Virgibacillus)。研究结果表明,岱山盐田土壤中存在较丰富的中度嗜盐菌种类,是筛选抗菌活性菌株的潜在资源库。 相似文献
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《中国科学院院刊(英文版)》2008,22(1):2-3
CAS should stick to the principle of rendering service to, and giving impetus for, the development of China＇s science enterprise by making S＆T innovations, said CAS President LU Yongxiang. The CAS president made the remarks in a recent talk to communicate the gist of the winter session of the Party＇s Leading-member Group at CAS, which was held from 7 to 11 January in Beijing. 相似文献
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《中国科学院院刊(英文版)》2008,22(1):13-14
Aresearch project on the greenhouse cultivation of Swertia chirayita Buch Ham, a popular herbal drug used by the Tibetan people against liver and gallbladder diseases, recently passed the acceptance check by a panel of experts under the auspices of Qinghai Provincial S＆T Bureau. The feat was accomplished by researchers from the Lanzhoubased CAS Northwest Institute of Plateau Biology. 相似文献
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The research into the origins of domestic animals is of significance not only for understanding their development per se but also for making clear the human society evolution. Although there are evidences to show that pigs were independently domesticated in a variety of places throughout the world the detailed scenario of the origin dispersal of domestic pigs in East Asia remains unclear.…… 《中国科学院院刊(英文版)》2008,22(1):7-7
The research into the origins of domestic animals is of significance not only for understanding their development per se, but also for making clear the human society evolution. Although there are evidences to show that pigs were independently domesticated in a variety of places throughout the world, the detailed scenario of the origin and dispersal of domestic pigs in East Asia remains unclear. 相似文献
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Among the galaxy of research development organizations in northwest Beijing's Zhongguancun district known as China's Silicon Valley two are especially well-known to IT researchers: Microsoft Research Asia 《中国科学院院刊(英文版)》2008,22(1):47-49
Among the galaxy of research and development organizations in northwest Beijing＇s Zhongguancun district, known as China＇s Silicon Valley, two are especially well-known to IT researchers： Microsoft Research Asia （MSRA）, and, within a few blocks on the other side of the street, the CAS Institute of Automation （CASIA）. While most people in Chinese scientific organizations strive to move to a large foreign counterpart, Dr. Stan Z. Li （LI Ziqing） moves against the stream. 相似文献
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《中国科学院院刊(英文版)》2008,22(1)
With great care, Dr. ZHOU Zhonghe takes out a package wrapped by cotton tissue from a drawer and says: "This is the gem of our collections: the fossil of a bird that lived 125 million years ago!" Then, pointing at a tiny mound, he explains: "Look, this is the claw and that is the head. It was in the egg shell and ready to hatch ... The species fell into a family of waterside inhabitants." 相似文献
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《中国科学院院刊(英文版)》2008,22(1):58-58
As a key component of the national initiative for basic S＆T data and infrastructure development, a nationwide survey on the lake water quality, volume and bio-resources has recently been kicked off. The move marks the systematic implementation of the secondround investigation of the country＇s lacustrine resources. 相似文献
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Bats the only mammals known to possess the real ability to fly have fascinated zoologists for a long time with their long-distance migration. How do they find their way home from an altitude of kilometers above sea level where their stunt of ultrasonic echolocation does not work?…… 《中国科学院院刊(英文版)》2008,22(1):3-4
Bats, the only mammals known to possess the real ability to fly, have fascinated zoologists for a long time with their long-distance migration. How do they find their way home from an altitude of kilometers above sea level, where their stunt of ultrasonic echolocation does not work？ Recent discoveries supported the theory that bats might be able to use magnetic clues to figure out their own position,just like what birds do. However, the latest results from a joint study by a group of researchers from the CAS, East China Normal University and University of Auckland reveal that bats apply an interesting strategy different from the one underlying birds＇ orientation technique, though they both are believed to derive information about their position from the subtle changes in the magnetic field of the Earth. 相似文献
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《中国科学院院刊(英文版)》2014,(3):252-252
<正>On February 12,2014,the international academic journal Cancer Research published an article online entitled"Latency Associated Nuclear Antigen of Kaposi's Sarcoma Associated Herpesvirus Promotes Angiogenesis through Targeting Notch Signaling Effector Hey1",as a result from the research conducted by Prof.LAN Ke’s lab at the Institut Pasteur of Shanghai,Chinese Academy of Sciences. 相似文献
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SONG Jianlan 《中国科学院院刊(英文版)》2014,(3):217-220
<正>Scientists from CAS and the Max Planck Society(MPG)met on May 13,2014 in Beijing to celebrate their four-decade-long ST cooperation and cherished friendship,anticipating further development of the bilateral relation.This partnership formally began four decades ago with a visit paid by then MPG President,Prof.Lüst to CAS,introduced CAS President BAI in his address at the celebration:"During the visit,CAS and MPG confi rmed the basic principles of cooperation,that is equality and mutual benefi ts for both sides,and reached agreement to develop a collaborative partnership."This occurred only two years after the establishment of the diplomatic relations between Germany and China,despite the shadow of the Cold War."Building up a partnership between Germany and 相似文献
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TANG Kun 《中国科学院院刊(英文版)》2014,(2):161-165
<正>The scientifi c goals of the Partner Group are to utilize large scale genomic polymorphism data to make fi ne inferences about human demographic history and various forms of natural selection in the human genome;and to understand the genetic basis and evolutionary mechanisms underlying the common variations in human facial morphology,both within and between populations. 相似文献